• Korean Journal of Medicinal Crop Science
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• v.16 no.6
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• pp.421-426
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• 2008

Three year-old peony (Paeonia. lactiflora Pall. cv. Taebaek) was cultivated in green-house at Jan. 15, Feb. 15, or Mar. 15, respectively. The mean of temperature during the forcing cultivation was higher (air; $1.0{\sim}11.1^{\circ}C$, soil; $1.1{\sim}7.4^{\circ}C$) than that of open-field condition. From sprouting to flowering in peony cultivated at Jan. 15 was about 54 days, which is shorted the cut flower periods (ca. 26 days) compared with the open-field cultivation. However, earlier forcing cultivars were very susceptible to pathogens such as powdery mildew or gray mold. The yield in green house was also lower than in the open-field cultivation. The content of bioactive compounds such as paeoniflorin and albiflorin in green-house cultivars was similar that of open-field cultivars. These results showed the forcing cultivation time of peony at Feb. 15 in green-house was most desirable for commercialization.

• Korean Journal of Medicinal Crop Science
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• v.6 no.3
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• pp.198-203
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• 1998

This experiment was conducted to determine the effect of different size of divided crowns used as planting material on the growth characteristics, yield and quality of Paeania lactiflora Pallas (P. lactiflora Pallas). The ratio of sprouting, rooting, and growth of above-ground parts increased with the increase of crown size. The root yields of under-ground parts from different size of divided crown were 2,674kg/10a for large size and 2,304kg/10a for medium size and the yield index was improved by 39% and 30%, respectively, compared to 1,921kg/10a in small size. Commodity ratio of roots over 10mm in diameter were higher for large and medium size crown: index increased by 26 % and 11 %, respectively, compared to 1,406kg/10a in small size. Income analyzed by crown size was \ 1,133,000 per 10a at the use of small crown size but income were increased 25 % and 48 %, respectively. by using medium and large size crown as compared with small crown.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.3
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• pp.199-204
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• 2001

The study was carried out to find effects on growth and yield by drought in Paeonia lactiflora Pallas. The water stress treatment was imposed artificially on April 1 to May 30(top part growth high stage), June 1 to July 30(root growth high stage), August 1 to September 29(late growth stage) and control(below 50kPa) in rain shelter. In drought periods of April 1 to May 30, stem length and number of stem were 67.5cm and 10.4 ea/plant. It showed poor growth compared with control, and root length and diameter also decreased to 27.2cm and 24.5mm, respectively. In root yield, drought treatment of June 1 to July 30 showed lower root yield as $1,809kg\;10a^{-1}$, $1,902kg\;10a^{-1}$ for drought period of August 1 to September 29, compared with $2,039kg\;10a^{-1}$ of control, resulting in 11% and 7% reduction, respectively. Optimum irrigation times (50kPa) was 17 days after continuos rain-off in Spring(April 1 to May 30), 9 days in summer(June 1 to July 30). It could be estimated that effective irrigation point was at least 13 days in after August(Aug. 1 to Sep. 29).

• Journal of the East Asian Society of Dietary Life
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• v.12 no.1
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• pp.23-31
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• 2002

The antioxidative activity was measured on the substances of water and ethanol soluble extract from Astragalus membranaceus Bunge, Chrysanthemum morifolium Ramat, Lycium chinensis Miller, Glycyrrhiza uralensis Fischer, Angelica gigas Nakai, Zizyphus jojoba Miller, Paeonia lactiflora Pallas, Cnidium officinale Makino by four different in vitro experimental models of DPPH (a,a'-diphenyl-$\beta$-picrylhydrazyl) method, superoxide dismutase like activity, thiocyanate method, and TBARS (thiobarbituric acid reactive substances) method. The Lycium chinensis Miller contained the highest amount of polyphenolic compounds. The electron donating ability of water extract from Glycyrrhiza uralensis Fischer and ethanol extract from Chrysanthemum were higher than those of the others. The superoxide dismutase-like activity of water extract from Astragalus membranaceus Bunge was the highest among those of all the others. The water extract from Zizyphus jujuba Miller showed the highest antioxidative activity determined by TBARS method. Compared to the control, the inducing period associated with the oxidation degree was delayed up to 8 days in both the water extract from Chrysanthemum, Lycium chinensis Miller, Glycyrrhiza uralensis Fischer, and Paeonia lactiflora Pallas and the in ethanol extract from Chrysanthemum and Glycyrrhiz uralensis Fischer. These results support that water and ethanol extracts from 8 kinds of medicinal herbs contain antioxidative compounds.

• Korean Journal of Medicinal Crop Science
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• v.6 no.3
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• pp.193-197
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• 1998

This experiment was carried out to determine the proper time for flower bud removing to improve growth, yield and paeoniflorin content of root in peony(Paeonia lactiflora Pallas). The flower buds removement caused short stem length by $7{\sim}9cm$ compared with control, but number of stem and stem diameter were similar to that of control. The number of root, fresh root weight and paeoniflorin content in peony increased when their flower buds were removed at earlier stages. The root dry weight was highest of 1.647kg/10a, and increased by 27% compared with control when their flower buds were removed at early stage (bud diameter was less than 10mm). The root dry weight increased by 13% and 10%, respectively. when their flower buds were removed prior to flowering and at flowering stage. Paeoniflorin content in leaf was higher than that in stem, and was higher at early stage. Leaf and stem showed higher paeonif1orin content when their flower buds were removed.

• Korean Journal of Acupuncture
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• v.39 no.2
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• pp.43-53
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• 2022

Objectives : Paeonia lactiflora Pallas (PLP) have been reported to have pharmacological effects such as anti-inflammatory and analgesic. However, it is not yet known whether PLP extract has anti-inflammatory effect on HaCaT cells, human keratinocyte. Methods : To confirm the anti-inflammatory effect of PLP on keratinocyte, TNF-𝛼/IFN-𝛾-stimulated HaCaT cells were used. HaCaT cells were pre-treated with PLP for 1h before stimulation with TNF-𝛼/IFN-𝛾. Then HaCaT cells were stimulated with TNF-𝛼/IFN-𝛾 for 24 h, the cells and media were harvested to measure the inflammatory cytokines levels. Granulocyte-macrophage colony stimulating factor (GM-CSF), monocyte chemoattractant protein-1 (MCP-1), interleukin 1 beta (IL-1𝛽), and TNF-𝛼 were analyzed by enzyme-linked immunosorbent assay (ELISA), and the mRNA expression of thymus and activation-regulated chemokines (TARC), IL-6, and IL-8 were measured by reverse transcription-polymerase chain reaction (RT-PCR). We also investigated the inhibitory mechanism of the mitogen-activated protein kinase (MAPKs) including ERK, JNK, and p38 and nuclear factor-kappaB (NF-𝜅B) by PLP using western blot. Results : PLP did not show cytotoxicity in HaCaT cells. In TNF-𝛼/IFN-𝛾-stimulated HaCaT cells, PLP significantly inhibited the expression of GM-CSF, MCP-1 IL-1𝛽, TNF-𝛼, TARC and IL-6. PLP inhibited the phosphorylation of ERK and translocation of NF-𝜅B into the nucleus. Conclusions : These results indicate that PLP could ameliorate the TNF-𝛼/IFN-𝛾-stimulated inflammatory response through inhibition of MAPK and NF-kB signal pathway. This suggests that PLP could be used beneficial agent to improve skin inflammation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.3
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• pp.215-219
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• 2006

This study was conducted to find to change of component by different species, plant parts and growth stage of Paeonia lactiflora Pallas Among the species of peony, the contents of compounds was higher in cultivated peony (P. lactiflora P.) as compared with wild peony (P. japonica M., P. obobata M., P. anomala L.). Amount of methyl gallate was highest in 0.45% at Euisung jakyak. Amount of compounds in peony was the highest in 1.0% at paeoniflorin, followed by methyl gallate, astragalin and kaempferol in order. Contents of compounds with different growth stage were observed highest in April, and showed decreased trend in the later growth stage. Methyl gallate was present in 1.79% at petal, 0.56% at leaf and 0.01% at root, astragalin present at 0.27% at petal, 0.20% at leaf and 0.03% at root, and paeoniflorin present at 0.43% at petal, 1.09% at leaf and 2.52% at root.

• Korean Journal of Medicinal Crop Science
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• v.7 no.1
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• pp.27-30
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• 1999

This experiment was carried out to determine the adequate machine peeling and retting time of peony roots, which are used as medicinal resources in Korea. When the roots were peeled for $10{\sim}30$ minitues in the peeling machine, the epidermis of root was removed, and higher yield was obtained and paeoniflorin content as compared with roots which were peeled for longer time. The paeoniflorin content of root varied with the different parts of root. The highest content was recorded in the upper part and the lowest in the middle part. The loss of paeoniflorin increased in the roots processed for expanded peeling and retting time. Lightness of peeled root was higher and ${\Delta}\;E$ was lower in the roots which were peeled by machine for longer time.

• Korean Journal of Medicinal Crop Science
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• v.18 no.1
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• pp.40-45
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• 2010

The application of PCR analysis on the herbal medicine Moutan Cortex (Paeonia suffruticosa Andrews) was evaluated by the comparison of the genetic relationship based on the DNA sequence with Paeoniae Radix (Paeonia lactiflora Pallas) following development of specific primers. Moutan Cortex and Paeoniae Radix were distinguished through the PCR analysis based on the internal transcribed spacer (ITS-PCR) from nuclear ribosomal DNA region. The 294 bp PCR products both of Moutan Cortex and Paeoniae Radix was amplified by MIF1 and MIR1. And a Moutan Cortex specific 225 bp PCR amplification product was amplified by MIF2 and MIR1 primers. The 225 bp sequence could be successfully amplified from Mortan Cortex of dried herbal preparations. PCR analysis based on ITS (ITS-PCR) may be an efficient tool for the discrimination of Moutan Cortex.

• 한국약용작물학회:학술대회논문집
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• 2011.09a
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• pp.140-141
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• 2011