Browse > Article

ITS-PCR Analysis for the Discrimination of Moutan Cortex  

Lee, Jae-Woong (Department of Agriculture, Chungnam National University)
Kim, Young-Hwa (Korea Institute of Oriental Medicine)
Ko, Byoung-Seob (Korea Institute of Oriental Medicine)
Ryuk, Jin-Ah (Korea Institute of Oriental Medicine)
Oh, Seung-Eun (Department of Biological Sciences, Konkuk University)
Park, Sang-Un (Department of Agriculture, Chungnam National University)
Lee, Mi-Young (Korea Institute of Oriental Medicine)
Publication Information
Korean Journal of Medicinal Crop Science / v.18, no.1, 2010 , pp. 40-45 More about this Journal
Abstract
The application of PCR analysis on the herbal medicine Moutan Cortex (Paeonia suffruticosa Andrews) was evaluated by the comparison of the genetic relationship based on the DNA sequence with Paeoniae Radix (Paeonia lactiflora Pallas) following development of specific primers. Moutan Cortex and Paeoniae Radix were distinguished through the PCR analysis based on the internal transcribed spacer (ITS-PCR) from nuclear ribosomal DNA region. The 294 bp PCR products both of Moutan Cortex and Paeoniae Radix was amplified by MIF1 and MIR1. And a Moutan Cortex specific 225 bp PCR amplification product was amplified by MIF2 and MIR1 primers. The 225 bp sequence could be successfully amplified from Mortan Cortex of dried herbal preparations. PCR analysis based on ITS (ITS-PCR) may be an efficient tool for the discrimination of Moutan Cortex.
Keywords
ITS-PCR Analysis; Nuclear DNA; Moutan Cortex; Paeoniae Radix;
Citations & Related Records
Times Cited By KSCI : 10  (Citation Analysis)
연도 인용수 순위
1 Lee MY, Doh EJ, Kim ES, Kim YW, Ko BS and Oh SE. (2008). Application of the multiplex PCR method for discrimination of Artemisia iwayomogi from other Artemisia herbs. Biological and Pharmaceutical Bulletin. 31:685-690.   DOI   ScienceOn
2 An SM, Ryuk JA, Kim YH, Chae BC, Kim HJ, Kim KH, Kang KK, Ko BS and Lee MY. (2006). Genetic analysis of Polygonati Rhizoma and Polygonati Odorati Rhizoma using random amplified microsatellite polymorphism. Korean Journal of Medicinal Crop Science. 14:125-129.   과학기술학회마을
3 Baigalmaa J, Kim MK, Noh JH, Sun H and Yang DC. (2009). Phylogenetic analysis of Schizonepeta Spike on the basis of DNA sequences. Korean Journal of Medicinal Crop Science. 17:46-53.   과학기술학회마을
4 Aimi N, Inaba M, Watanabe M and Shibata S. (1969). Chemical studies on the oriental plant drugs-XXIII : Paeoniflorin, a glucoside of Chinese paeony root. Tetrahedron. 25:1825-1838.   DOI   ScienceOn
5 Wang B, Pang Z and Zhang Q. (1994). Chemiluminescence in the study of paeonol. Chinese Pharmaceutical Journal. 29:35-38.
6 Park SM, Jun DW, Park CH, Jang JS, Park SK, Ko BS, Kim BJ and Choi SB. (2004). Hypoglycemic effects of crude extracts of Moutan Radicis Cortex. Korean Journal of Food Science and Technology. 36:472-477.   과학기술학회마을
7 Sang T, Crawford DJ and Stuessy TF. (1995). Documentation of reticulate evolution in peonies (Paeonia) using internal transcribed spacer sequences of nuclear ribosomal DNA: implications for biogeography and concerted evolution. Proceedings of the National Academy of Sciences. 92:6813-6817.   DOI   ScienceOn
8 Tank DC and Sang T. (2001). Phylogenetic utility of the glycerol- 3-phosphate acyltransferase gene : Evolution and implications in Paeonia (Paeoniaceae). Molecular Phylogenetics and Evolution. 19:421-429.   DOI   ScienceOn
9 White TJ, Bruns T, Lee S and Taylor J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocol: A guide to method and applications. 315-322. Academic Press. San Diego, USA.
10 You JK, Chung MJ, Kim DJ, Seo DJ, Park JH, Kim TW and Choe M. (2009). Antioxidant and tyrosinase inhibitory effects of Paeonia suffruticosa water extract. Journal of the Korean Society of Food Science and Nutrition. 38:292-296.   과학기술학회마을   DOI
11 Kim SJ, Park JH, Choi SY, Son KH and Kim KU. (2007). Isolated and identification of biological activity compounds from leaves and stem of Paeonia lactiflora Pallas. Korean Journal of Medicinal Crop Science. 15:6-11.   과학기술학회마을
12 Li G, Seo CS, Lee KS, Kim HJ, Chang HW, Jung JS, Song DK and Son JK. (2004). Protective constituents against sepsis in mice from the root cortex of Paeonia suffruticosa. Archives of Pharmacial Research. 27:1123-1126.   과학기술학회마을   DOI
13 Li Q. (1988). Advances in the pharmacology of the root bark of Paeonia suffruticosa. Chinese Traditional and Herbal Drugs. 19:276-278.
14 Lin HC, Ding HY, Ko FN, Teng CM and Wu YC. (1999). Aggregation inhibitory activity of minor acetophenones from Paeonia species. Planta Medica. 65:595-599.   DOI   ScienceOn
15 Lu KT, Lee HC, Liu FS, Lo CF and Lin JH. (2009). Discriminating Astragali Radix from Hedysarum Radix in Chinese medicine preparations using nested PCR and DNA sequencing methods. Journal of Food and Drug Analysis. 17:380-385.
16 Kim SH, Kim SA, Park MK, Kim SH, Park YD, Na HJ, Kim HM, Shin MK and Ahn KS. (2004). Paeonol inhibits anaphylactic reaction by regulating histamine and TNF-a. International Immunopharmacology. 4:279-287.   DOI   ScienceOn
17 Kim JS, Kim YJ, Lee SY and Kang SS. (2008). Phytochemical studies on Paeoniae Radix (3) : Triterpenoids. Korean Journal of Pharmacognosy. 39:37-42.   과학기술학회마을
18 Lee MS. (1976). Studies on the breeding of new variety of cultivated Paeonia. WonKwang University. 10:481-502.
19 Kim HK, Chang KW, Choi KJ and Ko SR. (2002). Comparison of quality and ecological properties Radix and Moutan Cortex, which were Paeonia Radix grafted with Moutan stem. Journal of the Korean Society Agricultural Chemistry and Biotechnology. 45:173-178.   과학기술학회마을
20 Kim JH, Sohn UI, Lee HC, Kim JC and Park SD. (1997). Phylogenetic analysis of herbaceous peony using ribosomal DNA partial sequencing and RAPDs. Korean Journal of Breeding. 29:349-358.
21 Chung JW, Lee GA, Lee SS, Bang KH, Park CB and Park YJ. (2009). Cultivar discrimination of Korean and Chinese Boxthorn (Lycium chinense Mill. and Lycium barbarum L.) using SSR markers. Korean Journal of Medicinal Crop Science. 17:445-451.   과학기술학회마을
22 Dai M, Zhi X, Peng D and Liu Q. (1999). Inhibitory effect of paeonol on experimental atherosclerosis in quails. Zhongguo Zhong Yao Za Zhi. 24:488-490.
23 Choi MY and Park HJ. (2005). In vitro antiinflammatory activity of paeonol from the essential oil and its derivative methylpaeonol. Korean Journal of Pharmacognosy. 36:116-120.   과학기술학회마을
24 Ghada B, Olfa S, Khaled C, Messaoud M, Mohamed M, Mokhtar T and Amel SH. (2009). Sequence analysis of the internal transcribed spacers (ITSs) region of the nuclear ribosomal DNA (nrDNA) in fig cultivars (Ficus carica L.). Scientia Horticulturae. 120:34-40.   DOI   ScienceOn
25 Gulbitti-Onarici S, Sancak C, Sumer S and Ozcan S. (2009). Phylogenetic relationships of some wild wheat species based on the internal transcribed spacer sequences of nrDNA. Current Science. 96:794-800.
26 Baldwin BG and Markos S. (1998). Phylogenetic utility of the external transcribed spacer (ETS) of 18S-26S rDNA: congruence of ETS and ITS trees of Calycadenia (Compositae). Molecular Phylogenetics and Evolution. 10:449-463.   DOI   ScienceOn
27 Akata Y, Kawano S and Tanase Y. (1980). High-speed liquid chromatographic analysis of drugs XII. Determination of paeoniflorin in pharmaceutical preparations including paeony root. Yakugaku Zasshi. 100:958-961.   DOI
28 Chou TC. (2003). Anti-inflammatory and analgesic effects of paeonol in carrageenan-evoked thermal hyperalgesia. British Journal of Pharmacology. 139:1146-1152.   DOI   ScienceOn