Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Effect of Paeonia Lactiflora Pallas on Atopic Dermatitis-Related Inflammation in HaCaT Cell

작약이 HaCaT 세포에서 아토피 피부염 관련 염증 억제에 미치는 영향

  • Lee, Hye-In (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Kim, Eom Ji (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Son, Dongbin (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Joo, Byung Duk (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Sohn, Youngjoo (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Kim, Eun-Young (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Jung, Hyuk-Sang (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
  • 이혜인 (경희대학교 한의과대학 해부학교실) ;
  • 김엄지 (경희대학교 한의과대학 해부학교실) ;
  • 손동빈 (경희대학교 한의과대학 해부학교실) ;
  • 주병덕 (경희대학교 한의과대학 해부학교실) ;
  • 손영주 (경희대학교 한의과대학 해부학교실) ;
  • 김은영 (경희대학교 한의과대학 해부학교실) ;
  • 정혁상 (경희대학교 한의과대학 해부학교실)
  • Received : 2022.04.04
  • Accepted : 2022.05.05
  • Published : 2022.06.27
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Abstract

Objectives : Paeonia lactiflora Pallas (PLP) have been reported to have pharmacological effects such as anti-inflammatory and analgesic. However, it is not yet known whether PLP extract has anti-inflammatory effect on HaCaT cells, human keratinocyte. Methods : To confirm the anti-inflammatory effect of PLP on keratinocyte, TNF-𝛼/IFN-𝛾-stimulated HaCaT cells were used. HaCaT cells were pre-treated with PLP for 1h before stimulation with TNF-𝛼/IFN-𝛾. Then HaCaT cells were stimulated with TNF-𝛼/IFN-𝛾 for 24 h, the cells and media were harvested to measure the inflammatory cytokines levels. Granulocyte-macrophage colony stimulating factor (GM-CSF), monocyte chemoattractant protein-1 (MCP-1), interleukin 1 beta (IL-1𝛽), and TNF-𝛼 were analyzed by enzyme-linked immunosorbent assay (ELISA), and the mRNA expression of thymus and activation-regulated chemokines (TARC), IL-6, and IL-8 were measured by reverse transcription-polymerase chain reaction (RT-PCR). We also investigated the inhibitory mechanism of the mitogen-activated protein kinase (MAPKs) including ERK, JNK, and p38 and nuclear factor-kappaB (NF-𝜅B) by PLP using western blot. Results : PLP did not show cytotoxicity in HaCaT cells. In TNF-𝛼/IFN-𝛾-stimulated HaCaT cells, PLP significantly inhibited the expression of GM-CSF, MCP-1 IL-1𝛽, TNF-𝛼, TARC and IL-6. PLP inhibited the phosphorylation of ERK and translocation of NF-𝜅B into the nucleus. Conclusions : These results indicate that PLP could ameliorate the TNF-𝛼/IFN-𝛾-stimulated inflammatory response through inhibition of MAPK and NF-kB signal pathway. This suggests that PLP could be used beneficial agent to improve skin inflammation.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (No. 2020R1A2C2005836), and the Ministry of Education (No. 2021R1I1A1A01043143).

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