Browse > Article
http://dx.doi.org/10.14406/acu.2022.007

Effect of Paeonia Lactiflora Pallas on Atopic Dermatitis-Related Inflammation in HaCaT Cell  

Lee, Hye-In (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Kim, Eom Ji (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Son, Dongbin (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Joo, Byung Duk (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Sohn, Youngjoo (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Kim, Eun-Young (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Jung, Hyuk-Sang (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Publication Information
Korean Journal of Acupuncture / v.39, no.2, 2022 , pp. 43-53 More about this Journal
Abstract
Objectives : Paeonia lactiflora Pallas (PLP) have been reported to have pharmacological effects such as anti-inflammatory and analgesic. However, it is not yet known whether PLP extract has anti-inflammatory effect on HaCaT cells, human keratinocyte. Methods : To confirm the anti-inflammatory effect of PLP on keratinocyte, TNF-𝛼/IFN-𝛾-stimulated HaCaT cells were used. HaCaT cells were pre-treated with PLP for 1h before stimulation with TNF-𝛼/IFN-𝛾. Then HaCaT cells were stimulated with TNF-𝛼/IFN-𝛾 for 24 h, the cells and media were harvested to measure the inflammatory cytokines levels. Granulocyte-macrophage colony stimulating factor (GM-CSF), monocyte chemoattractant protein-1 (MCP-1), interleukin 1 beta (IL-1𝛽), and TNF-𝛼 were analyzed by enzyme-linked immunosorbent assay (ELISA), and the mRNA expression of thymus and activation-regulated chemokines (TARC), IL-6, and IL-8 were measured by reverse transcription-polymerase chain reaction (RT-PCR). We also investigated the inhibitory mechanism of the mitogen-activated protein kinase (MAPKs) including ERK, JNK, and p38 and nuclear factor-kappaB (NF-𝜅B) by PLP using western blot. Results : PLP did not show cytotoxicity in HaCaT cells. In TNF-𝛼/IFN-𝛾-stimulated HaCaT cells, PLP significantly inhibited the expression of GM-CSF, MCP-1 IL-1𝛽, TNF-𝛼, TARC and IL-6. PLP inhibited the phosphorylation of ERK and translocation of NF-𝜅B into the nucleus. Conclusions : These results indicate that PLP could ameliorate the TNF-𝛼/IFN-𝛾-stimulated inflammatory response through inhibition of MAPK and NF-kB signal pathway. This suggests that PLP could be used beneficial agent to improve skin inflammation.
Keywords
Paeonia Lactiflora Pallas; HaCaT; pro-inflammatory cytokine; atopic dermatitis; anti-inflammatory;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Avena-Woods C. Overview of atopic dermatitis. Am J Manag Care. 2017 ; 23(8 Suppl) : S115-S123.
2 Kim M, Kim J. Phenotypes and endotypes of atopic dermatitis: Clinical implications. Allergy Asthma Respir Dis. 2020 ; 8(1) : 9-14. https://doi.org/10.4168/aard.2020.8.1.9   DOI
3 Kim JY, Yang Y. Effects of Atopic Dermatitis on Suicidal Ideation, Plans and Attempts in Young Adult. J Korean Acad Community Health Nurs. 2017 ; 28(4) : 504-12. https://doi.org/10.12799/jkachn.2017.28.4.504   DOI
4 Zaidi Z, Lanigan SW. Skin: Structure and Function, Dermatology in Clinical Practice Springer. 2010: 1-15. https://doi.org/10.1007/978-1-84882-862-9_1   DOI
5 Homey B, Steinhoff M, Ruzicka T, Leung DYM. Cytokines and chemokines orchestrate atopic skin inflammation. J Allergy Clin Immunol. 2006 ; 118(1) : 178-89. https://doi.org/10.1016/j.jaci.2006.03.047   DOI
6 Avgerinou G, Goules AV, Stavropoulos PG, Katsambas AD. Atopic dermatitis: new immunologic aspects. Int J Dermatol. 2008 ; 47(3) : 219-24. https://doi.org/10.1111/j.1365-4632.2008.03471.x   DOI
7 Jung MR, Lee TH, Bang MH, Kim H, Son Y, Chung DK, et al. Suppression of thymus- and activation-regulated chemokine (TARC/CCL17) production by 3-O-β-D-glucopyanosylspinasterol via blocking NF-κB and STAT1 signaling pathways in TNF-α and IFN-γ-induced HaCaT keratinocytes. Biochem Biophys Res Commun. 2012 ; 427(2) : 236-41. https://doi.org/10.1016/j.bbrc.2012.08.087   DOI
8 Son KH, Lee JG, Jee SY. A philological study on demonstration of Atopic dermatitis. J East-West Med. 2009 ; 34(4) : 15-24.
9 Yun YH, Choi IH. A study on the Development of Traditional Korean Medicine Clinical Practice Guideline for Atopic Dermatitis. J Korean Med Ophthalmol Otolaryngol Dermatol. 2012 ; 25(2) : 38-48. https://doi.org/10.6114/JKOOD.2012.25.2.038   DOI
10 Lee SY, Yoon HD, Shin OC, Park CS. A Case Report of Skin Scale Treated with Hyulbuchukeotang. Korean J Oriental Int Med. 2005;26(3):720-4
11 He DY, Dai SM. Anti-inflammatory and immunomodulatory effects of paeonia lactiflora pall., a traditional chinese herbal medicine. Front Pharmacol. 2011 ; 2 : 10. https://doi.org/10.3389/fphar.2011.00010   DOI
12 Hu B, Xu G, Zhang X, Xu L, Zhou H, Ma Z, et al. Paeoniflorin Attenuates Inflammatory Pain by Inhibiting Microglial Activation and Akt-NF-κB Signaling in the Central Nervous System. Cell Physiol Biochem. 2018 ; 47(2) : 842-50. https://doi.org/10.1159/000490076   DOI
13 Becher B, Tugues S, Greter M. GM-CSF: From Growth Factor to Central Mediator of Tissue Inflammation. Immunity. 2016 ; 45(5) : 963-73. https://doi.org/10.1016/j.immuni.2016.10.026   DOI
14 Jin X, Wang J, Xia ZM, Shang CH, Chao QL, Liu YR, et al. Anti-inflammatory and Anti-oxidative Activities of Paeonol and Its Metabolites Through Blocking MAPK/ERK/p38 Signaling Pathway. Inflammation. 2016 ; 39(1) : 434-46. https://doi.org/10.1007/s10753-015-0265-3   DOI
15 Berke R, Singh A, Guralnick M. Atopic dermatitis: an overview. Am Fam Physician. 2012 ; 86(1) : 35-42
16 Eckert RL. Structure, function, and differentiation of the keratinocyte. Physiol Rev. 1989 ; 69(4) : 1316-46. https://doi.org/10.1152/physrev.1989.69.4.1316   DOI
17 Cha KJ, Im MA, Gu A, Kim DH, Lee D, Lee JS, et al. Inhibitory effect of Patrinia scabiosifolia Link on the development of atopic dermatitis-like lesions in human keratinocytes and NC/Nga mice. J Ethnopharmacol. 2017 ; 206 : 135-43. https://doi.org/10.1016/j.jep.2017.03.045   DOI
18 Lim JY, Lee JH, Lee DH, Lee JH, Kim DK. Umbelliferone reduces the expression of inflammatory chemokines in HaCaT cells and DNCB/DFE-induced atopic dermatitis symptoms in mice. Int Immunopharmacol. 2019 ; 75 : 105830. https://doi.org/10.1016/j.intimp.2019.105830   DOI
19 Shi Y, Liu CH, Roberts AI, Das J, Xu G, Ren G, et al. Granulocyte-macrophage colony-stimulating factor (GM-CSF) and T-cell responses: what we do and don't know. Cell Res. 2006 ; 16(2) : 126-33. https://doi.org/10.1038/sj.cr.7310017   DOI
20 Kaburagi Y, Shimada Y, Nagaoka T, Hasegawa M, Takehara K, Sato S. Enhanced production of CC-chemokines (RANTES, MCP-1, MIP-1α, MIP-1β, and eotaxin) in patients with atopic dermatitis. Arch Dermatol Res. 2001 ; 293(7) : 350-5. https://doi.org/10.1007/s004030100230   DOI
21 Thompson WL, Van Eldik LJ. Inflammatory cytokines stimulate the chemokines CCL2/MCP-1 and CCL7/MCP-7 through NFkB and MAPK dependent pathways in rat astrocytes. Brain Res. 2009 ; 1287 : 47-57. https://doi.org/10.1016/j.brainres.2009.06.081   DOI
22 di Giovine FS, Duff GW. Interleukin 1: the first interleukin. Immunol Today. 1990 ; 11(1) : 13-20. https://doi.org/10.1016/0167-5699(90)90005-t   DOI
23 Lopez-Castejon G, Brough D. Understanding the mechanism of IL-1β secretion. Cytokine Growth Factor Rev. 2011 ; 22(4) : 189-95. https://doi.org/10.1016/j.cytogfr.2011.10.001   DOI
24 Popa C, Netea MG, van Riel PLCM, van der Meer JWM, Stalenhoef AFH. The role of TNF-α in chronic inflammatory conditions, intermediary metabolism, and cardiovascular risk. J Lipid Res. 2007 ; 48(4) : 751-62. https://doi.org/10.1194/jlr.R600021-JLR200   DOI
25 Neurath MF, Finotto S. IL-6 signaling in autoimmunity, chronic inflammation and inflammation-associated cancer. Cytokine Growth Factor Rev. 2011 ; 22(2) : 83-9. https://doi.org/10.1016/j.cytogfr.2011.02.003   DOI
26 Lu N, Malemud CJ. Extracellular Signal-Regulated Kinase: A Regulator of Cell Growth, Inflammation, Chondrocyte and Bone Cell Receptor-Mediated Gene Expression. Int J Mol Sci. 2019 ; 20(15) : 3792. https://doi.org/10.3390/ijms20153792   DOI
27 Amarbayasgalan T, Takahashi H, Dekio I, Morita E. Interleukin-8 content in the stratum corneum as an indicator of the severity of inflammation in the lesions of atopic dermatitis. Int Arch Allergy Immunol. 2013 ; 160(1) : 63-74. https://doi.org/10.1159/000339666   DOI
28 Johnson GL, Lapadat R. Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science. 2002 ; 298(5600) : 1911-2. https://doi.org/10.1126/science.1072682   DOI
29 Colombo I, Sangiovanni E, Maggio R, Mattozzi C, Zava S, Corbett Y, et al. HaCaT Cells as a Reliable In Vitro Differentiation Model to Dissect the Inflammatory/Repair Response of Human Keratinocytes. Mediators Inflamm. 2017 ; 2017 : 7435621. https://doi.org/10.1155/2017/7435621   DOI
30 Masuda K, Shima H, Katagiri C, Kikuchi K. Activation of ERK induces phosphorylation of MAPK phosphatase-7, a JNK specific phosphatase, at Ser-446. J Biol Chem. 2003 ; 278(34) : 32448-56. https://doi.org/10.1074/jbc.M213254200   DOI
31 Venuprasad K, Elly C, Gao M, Salek-Ardakani S, Harada Y, Luo JL, Yang C, Croft M, Inoue K, Karin M, Liu YC. Convergence of Itch-induced ubiquitination with MEKK1-JNK signaling in Th2 tolerance and airway inflammation. J Clin Invest. 2006 ; 116 : 1117-26. https://doi.org/10.1172/JCI26858   DOI
32 Lawrence T. The nuclear factor NF-κβ pathway in inflammation. Cold Spring Harb Perspect Biol. 2009 ;1(6) : a001651. https://doi.org/10.1101/cshperspect.a001651   DOI
33 Celec P. Nuclear factor kappa B--molecular biomedicine: the next generation. Biomed Pharmacother. 2004 ; 58(6-7) : 365-71. https://doi.org/10.1016/j.biopha.2003.12.015   DOI
34 Durand JK, Baldwin AS. Targeting IKK and NF-κB for Therapy. Adv Protein Chem Struct Biol. 2017 ; 107 : 77-115. https://doi.org/10.1016/bs.apcsb.2016.11.006   DOI
35 Kim SJ, Lee AR, Kim SH, Kim KJ, Kwon OJ, Choi JY, et al. Skin Wrinkle Improvement Effect of Paeoniae radix and processed Paeoniae radix Through inhibition of Advanced glycation end products (AGEs). Kor J Herbology. 2017 ; 32(4) : 53-60. https://doi.org/10.6116/KJH.2017.32.4.53   DOI
36 Kim KH, Choi YG, Hong YM, Yeo SJ, Choi JH, Kim TH. Gene expression microarray analysis of Paeoniae radix on IL-1β-stimulated primary human gingival fibroblast. J Korean Med. 2010 ; 31(2) : 91-108
37 Herbology Editorial Committee of Korean Medicine : Herbology. 2nd ed. Seoul : Younglimsa. 2009 ; 637-8.
38 Gong NM, Jee SY. A Literature study about comparison of Eastern-Weatern medicine on the Atopic dermatitis. J Korean Med Ophtahlmol Otolaryngol Dermatol. 1999 ; 12 (1) : 241-53.
39 Shimada Y, Takehara K, Sato S. Both Th2 and Th1 chemokines (TARC/CCL17, MDC/CCL22, and Mig/CXCL9) are elevated in sera from patients with atopic dermatitis. J Dermatol Sci. 2004 ; 34(3) : 201-8. https://doi.org/10.1016/j.jdermsci.2004.01.001   DOI
40 Saeki H, Tamaki K. Thymus and activation regulated chemokine (TARC)/CCL17 and skin diseases. J Dermatol Sci. 2006 ; 43(2) : 75-84. https://doi.org/10.1016/j.jdermsci.2006.06.002   DOI