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Biological Control of Root-knot Nematode by Streptomyces sampsonii KK1024 (Streptomyces sampsonii KK1024를 이용한 뿌리혹선충 (Root-knot nematode)의 생물학적 방제)

  • Kim, Sang-Su;Kang, Seon-I;Kim, Jin-Si;Lee, Yong-Sung;Hong, Sung-Hyun;Naing, Kyaw Wai;Kim, Kil-Yong
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.6
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    • pp.1150-1157
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    • 2011
  • Streptomyces sampsonii KK1024 having strong chitinolytic activity was isolated from crab-shell rich soil at Muan, Jeolanamdo. The KK1024 produced chitinase, protease, gelatinase and lipase. When 50% of KK1024 culture broth was treated to juveniles and eggs of root-knot nematode, juvenile mortality at 3 days was 81.67% and egg hatch rate at 5 days was 2.00%. When $183.7{\mu}g\;mL^{-1}$ of crude enzyme produced by KK1024 was treated, juvenile mortality at 3 days was 96.00% and egg hatch rate at 5 days was 5.33%. At 1% of butanol extract from KK1024, juvenile mortality was highest with 90.00% and egg hatch rate was lowest with 0%. The comparison of the effect of KK1024 culture broth with only medium, synthetic fertilizer, and commercial nematicide on tomato growth and nematode infection was examined in pot trials. KK1024 culture broth showed lower number of egg mass and gall in plant, and population of juveniles in soil compared with only medium and synthetic fertilizer treatment, but not in commercial nematicide. However, the highest shoot weight and length was discovered in KK1024 culture broth. These results suggest that Streptomyces sampsonii KK1024 producing lytic enzymes and nematicidal compounds can be one of candidates for biocontrol agents against root-knot nematodes.

Impact of Compost Application on Improvement of Rice Productivity and Quality in Reclaimed Soil (간척지 토양에서 퇴비처리가 벼의 생산 및 품질개선에 미치는 영향)

  • Moon, Young-Hun;Kwon, Young-Rip;Ahn, Byung-Koo;Kim, Dae-Hyang;Han, Seong-Soo
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.5
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    • pp.808-813
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    • 2011
  • This study was conducted to reduce the dependability of farmers on chemical fertilizers for rice cultivation. Soil chemical and biological properties were monitored before experiment and at the time of harvesting. The results showed that EC, available $SiO_2$, and exchangeable $Ca^{2+}$ were decreased at the time of harvesting while pH, OM, and exchangeable $K^+$ and $Mg^{2+}$ were remain unchanged, compared with soil before experiment. Population of aerobic bacteria, Bacillus sp., and fungi were also increased at the time of harvesting in the paddy field, compared with before fertilization, in the treatment of 50% soil-testing fertilizer+ 50% compost. Concentrations of N, P, and K in rice leaves increased with the fertilizers application, maximum increase was recorded in 50% soil-testing fertilizer+ 50% compost. Non-significant difference was observed in the morphological parameters of rice among the treatments. The chlorophyll contents of rice leaf increased in a similar fashion up to 60 days, thereafter, sharp decrease was observed in all the treatments. Maximum yield (per 10a) was recorded in the field treated with 50% soil-testing fertilizer+ 50% compost followed by standard applied fertilizer, 70% soil-testing fertilizer+ 30% compost, soil-testing fertilizer and unfertilized plot. Amylose content showed non-significant difference within the treatments. Protein content increased with the use of fertilizers and best protein content was recorded in the treatment of 50% soil-testing fertilizer+ 50% compost. It was concluded that the amount of the chemical fertilizer used was directly proportional to the protein content of rice grain. However, the palatability of rice grown in unfertilized field was better than the treatments but minimum yield was obtained. Hence, the treatment of 50% soil-testing fertilizer+ 50% compost, was the best among the fertilizer combinations for rice cultivation as supported by the yield, protein and palatability index.

Effects of Dietary Supplementation of Mulberry Leaves and Silkworm Excreta Extracts on Weight Performance, Blood Characteristics and Cecal Microflorae of Chicks (뽕잎과 잠분 추출물 급여가 육계의 생산성, 혈액 성상 및 맹장 내 미생물군에 미치는 영향)

  • Han, Sang-Hoon;Kim, Dong Wook;Ji, Sang Yoon;Hong, Seong Koo;Kim, Sang-Ho;Lee, Heui-Sam
    • Journal of Sericultural and Entomological Science
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    • v.50 no.2
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    • pp.150-160
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    • 2012
  • The object of this study was to evaluate the effects of dietary supplementation of mulberry leaves and silkworm excreta ethanol extracts on weight performances, blood characteristics, cecal microflorae of chickens. Two hundred forty male broiler chicks(Ross) were fed diets for five weeks containing 0.1%(MLA) and 1%(MLB) of mulberry leaves ethanol extracts, and 0.1%(SEA) and 1%(SEB) of silkworm excreta ethanol extracts. Weight performance did show no significant difference in all test groups which were fed with supplementation of mulberry leaves and silkworm excreta ethanol extracts. They showed better weight gain and feed conversion than the negative control group which was fed only with forage without antibiotics. ABTS(2'-azine-bis[3-ethylbenzothiazoline-6-sulfonic acid]) test was conducted to investigate free radical scavenging activity of blood in tested groups. ABTS scavenging activities of tested groups were higher than control groups in significant level, though there was no significant difference(P = 0.396). Specifically, MLB group showed the highest scavenging activity. Blood-level concentration of MDA, which is an indicator of lipid peroxidation, was also decreased in tested groups and the lowest level was observed in SEA(P = 0.001). As storage time increased at $4^{\circ}C$, muscle-level MDA concentrations of all tested groups were generally increased and significant difference was obsereved between tested groups and controls in total increase of MDA concentration($P=4.417{\times}10^{-3}$). In cecal microflorae, SEA and SEB showed decreased total microbe population compared to NC($P=6.462{\times}10^{-5}$) and even to PC. Supplementation of mulberry leave and silkworm excreta ethanol extract did show a similar inhibition effect against Salmonella sp., furthermore, MLB did enhanced the growth of Lactobacillus sp.($P=3.636{\times}10^{-7}$). In summary, ethanol extract of silkworm excreta may be a potential alternative of antibiotics for chicks. In addition, both of ethanol extracts supplementation to broiler chicks would be very useful not only to improve antioxidant effect of blood but also to suppress lipid peroxidation without any loss of weight performance in poultry farming.

The Relationships Among Highly Caffeinated Beverage Intake and Depressive Symptom, Suicide in Adolescents (청소년의 고카페인 음료 섭취와 우울증상 및 자살의 관계)

  • Ahn, In-Young;Seo, Ji-Yeong;Lee, Dongyun;Lee, So-Jin;Cha, Boseok;Kim, Bong-Jo;Park, Chul-Soo;Choi, Jae-Won;Lee, Cheol-Soon
    • Korean Journal of Psychosomatic Medicine
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    • v.24 no.2
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    • pp.191-199
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    • 2016
  • Objectives : Despite the increased popularity of highly caffeinated beverages, there is little research examining psychiatric adverse effects. The purpose of this study was to investigate the relationships among pattern of highly caffeinated beverage intake and depressive symptom, suicidal ideation, suicidal plan, suicidal attempt in Korean adolescents. Methods : The data was obtained from the 2014 Korean Youth's Risk Behavior Web-based Study by Korea Centers for Disease Control & Prevention. All participants conducted web-based questionnaire survey. Chisquare test and multiple logistic regression analysis were performed to determine the association among highly caffeinated beverage intake pattern, depressive symptom, suicidal ideation, suicidal plan and suicidal attempt adjusting for differences in age, gender, academic achievement, socioeconomic status. Results : A total of 71,638 participants were enrolled in this study. Depressive symptom, suicidal ideation, suicidal plan and suicidal attempt were significantly more frequent in the group with presence of highly caffeinated beverage intake within 1 week than in non-drinker group(p<0.01). Highly caffeinated beverage intake was significantly associated with suicidal attempt(OR=1.99 ; 95% CI, 1.77-2.22). In addition, depressive symptom, suicidal ideation, suicidal plan and suicidal attempt were significantly more common in the group with heavy-drinker who exceed recommended daily intake dose of caffeine than in the group with light-drinker(p<0.01). Heavy drinking of caffeinated beverage was significantly associated with suicidal attempt(OR=4.05 ; 95% CI, 3.02-5.43). Conclusions : We found that highly caffeinated beverage intake was related to more frequent depressive symptom, suicidal ideation, plan, attempt in adolescents. Also, caffeine intake which exceed recommended daily intake dose identified the predictor of suicidal attempt. Our result suggested that clinicians need to be aware of the possible psychiatric adverse effects of highly caffeinated beverage in vulnerable population including young adolescents.

Differential Association of History of Premenstrual Syndrome/Premenstrual Dysphoric Disorder with Vasomotor Symptoms According to Menopausal Stage (폐경 단계에 따른 월경전 증후군/월경전 불쾌장애의 과거력과 혈관운동증상의 차별적 연관성)

  • Hyun, Hong-Dae;Joe, Sook-Haeng;Jeong, Hyun-Ghang;Ko, Young-Hoon;Kwon, Eunjoo;Kim, Hyekyeong;Ko, Seung-Duk
    • Korean Journal of Psychosomatic Medicine
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    • v.23 no.1
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    • pp.57-65
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    • 2015
  • Objectives:Within the normal reproductive cycles of women, dramatic fluctuations of sexual hormones occur in the premenstrual and menopausal periods. In both periods, women are vulnerable to mood disturbances and show several somatic complaints. Based on these common clinical profiles and physiological changes, a relationship between vasomotor symptoms and the premenstrual syndrome has been suggested. However, attempts to establish such a link have yielded inconclusive results. The purpose of this study was to investigate the association between histories of premenstrual syndrome and menopausal vasomotor symptoms within different menopausal stages. Methods:This cross-sectional study recruited Korean women aged 45-64 years who were perimenopausal and postmenopausal from 16 branch offices of the Korean Association of Health Promotion. All subjects completed self-report questionnaires that asked about a history of premenstrual syndromes, vasomotor symptoms, and several other variables. Results:A total of 1054 participants(361 perimenopausal women and 693 postmenopausal women) completed the study. Severity of premenstrual symptoms significantly correlated with postmenopausal vasomotor symptoms, only in late perimenopausal(r=0.213, p=0.010) and early postmenopausal women(r=0.246, p<0.001). After adjusting for several factors related to vasomotor symptoms, a history of premenstrual syndrome was a significant predictor of moderate to very severe vasomotor symptoms in late perimenopausal(OR=5.197, p=0.005) and early postmenopausal women(OR=3.017, p=0.010). Conclusions:This study suggests that a history of premenstrual syndrome/premenstrual dysphoric disorder is differentially associated with vasomotor symptoms in the menopausal stage. Prospective studies with larger population are needed to confirm these findings

Studies on Distribution and Ecology of Clithon retropictus (Martens, 1879) in South Korea (한국산 기수갈고둥의 분포 및 생태에 관한 연구)

  • Han, Seung Pil;Hwang, In Chul;Kwon, Soon Jik
    • Journal of Wetlands Research
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    • v.23 no.4
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    • pp.317-326
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    • 2021
  • The present study was carried out with a view to understand the distribution and ecology of Clihton retropictus (Martens, 1879), a class II endangered wildlife designated and protected by the Ministry of Environment, Republic of Korea, and to suggest the effective conservation and management measures for this species and its habit. First, the distribution of Clihton retropictus was investigated nationwide, and environmental factors such as salinity and substratum were analyzed in consideration of the ecological characteristics of this species. In addition, the individual size, density, and movement of Clihton retropictus per each district surveyed were analyzed. Due to its nature of inhabiting in the brackish water zone, the analysis showed that the salinity of this species was 0.7 ~ 16.6‰ at low tide and 1.0 ~ 17.3% at high tide. It was learned from the analysis of substratum that its composition in each river under investigation was slightly different, but most of substratum had a higher composition ratio than gravel (pebble, 16 ~ 64 mm). On the other hand, the size of each individual was checked by measuring the length of its diameter, and there was almost no change in the individual when the inhabited range of Clihton retropictus was short. However, there was tendency that the longer the habitat range, the bigger the individual size. From surveying the population density by season, it was made clear that, during the summer (July to August) and fall (September to October), the density was relatively higher while the number of individuals decreased significantly during winter (November to December) when the water temperature was lowered. In an effort to effectively establish conservation and management measures, the moving distance was measured, and it became known that, during the surveys in summer and fall when the individuals were active, the individuals moved in more distances than during the winter survey. The results of this study are expected to be utilized as basic data for establishing effective management plans such as creation of habitats for Clihton retropictus, its migration projects and selection of migration destinations which get unavoidable due to development projects.

A Study on the Effect of Startup's Innovation Orientation on Growth Aspiration (창업기업의 혁신지향성이 성장열망에 미치는 영향에 관한 연구)

  • Oh, Hyemi;Lee, Chaewon;Kim, Jinsoo
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.16 no.5
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    • pp.1-14
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    • 2021
  • Innovation and Scale-up of Start-up companies are becoming important national tasks. In the past, it was spread the start-up policy paradigm such as 'Start-up America', 'Start-up Chile', 'Start-up Britain' to overcome the recession globally. However as the economic recovery has become more visible recently in advanced economies, it is shifting from a start-up support policy to a scale-up oriented policy paradigm such as 'Scale-up America', Scale-up UK', 'Scale-up Denmark'. It is necessary to enter the scale-up phase beyond the start-up phase to increase the number of high-quality jobs and to continue economic growth. Therefore, it is necessary to grow the start-up into a strong medium-sized company and to lay the foundation for survival. Therefore, the purpose of this study is to consider the antecedent factors that influence the scale-up aspiration for the start-up firm to grow into a scale-up company, and empirically identifies the differences between the stages of economic development and entrepreneurs in the country. In order to accomplish the purpose, this study predicted scale-up by aspiration which is a predictor of scale-up behavior because it is difficult to achieve visible growth in a short period of time due to the characteristics of start-up companies. In order to empirically explore these relationships, the data were collected from nascent entrepreneurs who have less than 3.5 years of the Adult Population Survey(APS) among the subjects surveyed by the Global Entrepreneurship Monitor(GEM) and the national economic development stage are divided into Innovation-driven, Efficiency-driven, Factor-driven type economies. For the test hypotheses, this study adopted the multi-level model analysis for comparison between national economic development stages and using the R 3.5.0 program. The results of this study are as follows. There is difference between the national economic development and the entrepreneur in the relationship between innovation orientation of entrepreneurs and scale-up aspirations. As the economy of the country develops, the innovation activity of the entrepreneur becomes more active. Since start-ups are heavily influenced by entrepreneurs, there is a difference in the degree of aspiration depending on how innovative an entrepreneur is in the same environment. In terms of the relationship between innovation orientation and scale-up aspiration, the fear of failure was found to differ between national economic development and entrepreneurs. The fear of failure differ from country to country, and this is one of the important factors affecting entrepreneurial activities. It is expected that the factors influencing the growth of the start-up companies which are identified through the results of these studies, will be used to create a suitable scale-up ecosystem according to the national economic development stage.

Distribution Prediction of Korean Clawed Salamander (Onychodactylus koreanus) according to the Climate Change (기후변화에 따른 한국꼬리치레도롱뇽(Onychodactylus koreanus)의 분포 예측에 대한 연구)

  • Lee, Su-Yeon;Choi, Seo-yun;Bae, Yang-Seop;Suh, Jae-Hwa;Jang, Hoan-Jin;Do, Min-Seock
    • Korean Journal of Environment and Ecology
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    • v.35 no.5
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    • pp.480-489
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    • 2021
  • Climate change poses great threats to wildlife populations by decreasing their number and destroying their habitats, jeopardizing biodiversity conservation. Asiatic salamander (Hynobiidae) species are particularly vulnerable to climate change due to their small home range and limited dispersal ability. Thus, this study used one salamander species, the Korean clawed salamander (Onychodactylus koreanus), as a model species and examined their habitat characteristics and current distribution in South Korea to predict its spatial distribution under climate change. As a result, we found that altitude was the most important environmental factor for their spatial distribution and that they showed a dense distribution in high-altitude forest regions such as Gangwon and Gyeongsanbuk provinces. The spatial distribution range and habitat characteristics predicted in the species distribution models were sufficiently in accordance with previous studies on the species. By modeling their distribution changes under two different climate change scenarios, we predicted that the distribution range of the Korean clawed salamander population would decrease by 62.96% under the RCP4.5 scenario and by 98.52% under the RCP8.5 scenario, indicating a sharp reduction due to climate change. The model's AUC value was the highest in the present (0.837), followed by RCP4.5 (0.832) and RCP8.5 (0.807). Our study provides a basic reference for implementing conservation plans for amphibians under climate change. Additional research using various analysis techniques reflecting habitat characteristics and minute habitat factors for the whole life cycle of Korean-tailed salamanders help identify major environmental factors that affect species reduction.

A Study of the Impact of Digital Capability and Personal Ability on the Intent to Continue Economic Activity : Focused on the Adjustment Effect of the Role Model (디지털 역량과 개인적 역량이 경제활동 지속 의도에 미치는 영향: 롤 모델의 조절 효과 중심으로)

  • Kim, Sang Jin;Ha, Kyu Soo
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.16 no.5
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    • pp.125-141
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    • 2021
  • The rapidly changing social structure of the digital environment is having a significant impact on economic activities. That is also an important issue for Individuals who want to sustain economic activities and countries that support policies. Non-face-to-face industries have been revitalized due to the problem of human capital utilization attributed to aging population, the real economic recession caused by Corona 19, contraction of face-to-face economic activities, reduction of employment, and job instability. Accordingly, digital media contents based economic activities have become commonplace, and the government's main policy issue is to use human capital effectively for media contents based economic activities. Adaptation to the digital environment has become a necessity, not a choice, for those who wish to continue to be in employment. Therefore, this study analyzed the effects of digital and individual abilities on intention to sustain the economic activity and verified the modulation effect of the role model. In order to achieve the purpose of this research, an online survey was conducted on men and women aged 20 to 80 nationwide, and 382 of the 385 collected questionaires were analyzed. The SPSS 23.0 program was used to analyze this study, and the questionaire questions were measured using the Likert 5-point scale. As a result of the analysis, first, the ability to utilize media contents in digital capacity has a positive impact on the intention to sustain economic activity, and that the higher the ability to utilize the latest digital media contents such as SNS, the more likely the intention to sustain economic activity. Secondly, it was found that the financial strength of individuals' abilities was affected by the negative impact, and that the experiences were affected by positive(+) impact on the intention to sustain economic activity. Thirdly, the social environment has no significant effect on the intention to sustain economic activity. Fourth, it was found that family support amongst social support has a positive impact on the intention to sustain economic activity, and that various emotional support for families has increased intention to sustain economic activity. Fifth, the role model was found to have a positive(+) impact on economic sustainability, while the ability to utilize media content and family support played a modulating role on economic sustainability. Therefore, as a result of this research, the government's policy support for employment and entrepreneurship is required in accordance with digital media content based digital education and human structure in order to sustain economic activities.

A Study on the Characteristics of Projects Following the Promotion of Private Park Special Projects (민간공원특례사업의 추진에 따른 사업특성에 관한 연구)

  • Gweon, Young-Dal;Park, Hyun-Bin;Kim, Dong-Pil
    • Journal of the Korean Institute of Landscape Architecture
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    • v.49 no.5
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    • pp.112-124
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    • 2021
  • This study was conducted to examine and analyze local governments, park status, project characteristics, and the implementation in detail for private park special projects across the country as a means of responding to the sunsetting of urban parks. As a result of the analysis, first, the private park special project, was found to be mainly implemented in cities with a population of more than 100,000, so there was a limit to the application on military installations or in local small cities. Therefore, rather than applying the special system collectively, it was judged that institutional flexibility, considering the characteristics and size of local government, was needed. Second, the current special projects by the park creation donation collection method shows monotonous development centered on apartment houses, so it is necessary to diversify the development by introducing a park preservation method that purchases and donates park sites. Third, it was found that the area standard needs to be eased to less than 50,000m2 to include parks with high utilization and good accessibility in urban areas of large cities, as the type and area of parks are limited. Fourth, most special projects are mountain parks, which are feared to damage the natural terrain and skyline, so separate ordinances should be established and applied, and development approaches should be made to allow nature and parks to coexist with the setting of detailed building guidelines for each type of facility. The guidelines should include, first, after the nationwide private park special projects are completed, standards for appropriate returns for similar projects should be established, institutional standards such as the recovery of excess profits should be established, and environmental reviews should be conducted. Second, it was found that local governments should institutionalize the composition of private consultations to promote the efficient management of projects through a cooperative system, and third, a roadmap for maintenance after the donation of special parks should be established.