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http://dx.doi.org/10.7745/KJSSF.2011.44.6.1150

Biological Control of Root-knot Nematode by Streptomyces sampsonii KK1024  

Kim, Sang-Su (Gwangju Science High School)
Kang, Seon-I (Chosun University Girl's High School)
Kim, Jin-Si (Chosun University Girl's High School)
Lee, Yong-Sung (Division of Applied Bioscience and Biotechnology, Institute of Agriculture Science and Technology, Chonnam National University)
Hong, Sung-Hyun (Division of Applied Bioscience and Biotechnology, Institute of Agriculture Science and Technology, Chonnam National University)
Naing, Kyaw Wai (Division of Applied Bioscience and Biotechnology, Institute of Agriculture Science and Technology, Chonnam National University)
Kim, Kil-Yong (Division of Applied Bioscience and Biotechnology, Institute of Agriculture Science and Technology, Chonnam National University)
Publication Information
Korean Journal of Soil Science and Fertilizer / v.44, no.6, 2011 , pp. 1150-1157 More about this Journal
Abstract
Streptomyces sampsonii KK1024 having strong chitinolytic activity was isolated from crab-shell rich soil at Muan, Jeolanamdo. The KK1024 produced chitinase, protease, gelatinase and lipase. When 50% of KK1024 culture broth was treated to juveniles and eggs of root-knot nematode, juvenile mortality at 3 days was 81.67% and egg hatch rate at 5 days was 2.00%. When $183.7{\mu}g\;mL^{-1}$ of crude enzyme produced by KK1024 was treated, juvenile mortality at 3 days was 96.00% and egg hatch rate at 5 days was 5.33%. At 1% of butanol extract from KK1024, juvenile mortality was highest with 90.00% and egg hatch rate was lowest with 0%. The comparison of the effect of KK1024 culture broth with only medium, synthetic fertilizer, and commercial nematicide on tomato growth and nematode infection was examined in pot trials. KK1024 culture broth showed lower number of egg mass and gall in plant, and population of juveniles in soil compared with only medium and synthetic fertilizer treatment, but not in commercial nematicide. However, the highest shoot weight and length was discovered in KK1024 culture broth. These results suggest that Streptomyces sampsonii KK1024 producing lytic enzymes and nematicidal compounds can be one of candidates for biocontrol agents against root-knot nematodes.
Keywords
Biological control; Streptomyces sampsonii KK1024; Root-knot nematodes; Butanol extract; Crude enzyme;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
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