• Korean Journal of Fisheries and Aquatic Sciences
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• v.49 no.5
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• pp.671-674
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• 2016

We cytogenetically analyzed a triploid King-Nupchi strain of the olive flounder Paralichthys olivaceus to define the simplest, most rapid, and most effective method of ploidy analysis in aquaculture farms. Female triploidy of the flounder King-Nupchi strain was induced by cold shock (3 min post-fertilization at 2-4℃ for 45 min). Triploid induction was confirmed by erythrocyte measurement (nuclear volume, 29.15±2.10 μm³); flow cytometry (2.14±0.03 pg/cell); chromosome count (3N=72); Ag-NOR banding; and silver staining. Silver staining of finned cells obtained using a solid tissue technique was the most effective method of ploidy verification.

• Microbiology and Biotechnology Letters
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• v.21 no.5
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• pp.428-434
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• 1993

Cloning and expression of two different crystal protein genes from transformed Bacillus thuringiensis were investigated. B. thuringiensis NT0423 is toxic to both Lepidopterous and Dipte-rous larvae. The pCG5 vector carrying crystal protein genes (mosquitocidal and hemolytic activity) of B. thurigiensis subsp. morrisoni PG-14 was transformed into B. thurigiensis NT0423. Transformant has expressed two type crystals of bipyramid from NT0423 and ovoid from pCG5 in one cell.

• Advances in pediatric surgery
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• v.6 no.1
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• pp.68-69
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• 2000

A case of primary hyperparathyroidism due to parathyroid adenoma is presented. A 14 year-old male was admitted to the hospital comptaining of voiding difficulty. The intravenous pyelogram demonstrated a stone in the proximal one third of the left ureter and marked hydronephrosis of the left kidney. The Tc-99m sestamibi nuclear scan demonstrated a hot spot below the lower pole of the left lobe of the thyroid. Laboratory study demonstrated hypercalcemia (12.4 mg/dL) and elevated parathyroid hormone (143.67 pg/mL). A parathyroid gland located below the lower pole of the left lobe of the thyroid was excised. A parathyroid adenoma, consisting of mainly chief cells was found on pathologic examination. Postoperatively the patient had transient hypocalcemic symptoms, which resolved with administration of calcium preparation and vitamin D.

• Journal of Species Research
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• v.13 no.2
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• pp.136-141
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• 2024

The purpose of this study is to isolate and identify wild yeasts from the soil samples collected in Daegu and Daejeon City, Republic of Korea. Among 15 strains isolated in this study, 13 strains were previously reported and two strains had not been reported in Republic of Korea. To identify wild yeast strains, pairwise sequence comparisons of D1/D2 region of the 26S rRNA gene sequence were done using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and assimilation tests were done using API 20C AUX kit. All strains were assigned to the phylum Basidiomycota. The two unrecorded yeast strains, PG2-2-10C and DJ2-14-10C, belong to the genus Holtermanniella (family Holtermanniaceae, order Holtermanniales, class Tremellomycetes) and Goffeauzyma (family Filobasidiaceae, order Filobasidiales, class Tremellomycetes), respectively. The two unrecorded yeast strains had oval shape and polar budding cells. This research describers the morphological and biochemical properties of the two unreported yeast species that had not officially reported in Korea.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.6
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• pp.862-867
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• 1999

The present study was undertaken to determine the effect of administration of exogenous progesterone early in gestation on uterine levels of IGF-I mRNA and on embryo survival at day 25 of gestation in the pig. Forty-one prepubertal gilts were induced into oestrus with PG600 and artificially inseminated at their subsequent naturally occurring oestrus. Gilts were then randomly assigned to one of three groups. Gilts in the two treatment groups were injected intramuscularly with 50 mg of progesterone either from day 2 to 14 (N=14) or from day 4 to 14 (N=15) after breeding while those in the control group (N=12) were given corn oil (0.5 ml) from day 2 to 14. Between days 25 and 28 of gestation, gilts were slaughtered and reproductive tracts were recovered. Endometrial tissue (1 g) was collected and analysed for IGF-I mRNA levels using a reverse transcription-polymerase chain reaction, Progesterone treatment, starting either on day 2 or 4 after breeding, neither significantly increased embryo survival rate by day 25 of gestation nor altered IGF-I mRNA levels in uterine tissue. However, across all samples, the IGF-I mRNA level in the uterus was highly correlated with embryo survival rate (r=0.8193, p<0.01), supporting the involvement of IGF-I in the regulation of porcine embryo development.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.127-127
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• 2003

본 연구의 목적은 젖소에서 다배란유기법의 확립을 위해서 호르몬을 처리법을 달리한 결과이다. 난포의 발생을 자극하는 FSH 호르몬을 하루에 2번의 호르몬 주사를 하는 데, 이는 혈액내에 난포자극호르몬의 농도를 연속적으로 유지하여 난소에서 다난포 발생의 목적을 달성하고자 하는 방법이다. 하지만 빈번하게 호르몬을 주사하는 방법은 처리하는 노력이 필요하고 공란우도 스트레스로 작용한다. 이러한 단점을 극복하기 위해 한번에 많은 난포의 성숙을 유도하는 다배란처리법에 대한 보고가 많이 있어, 본 연구에서 이 다배란처리 효과를 비교하고자 젖소를 이용하였다. 방법으로는 다회주사법(8회)과 일회주사법(1회)에서 황체수와 수정란의 생산수 등을 조사하였다. 다회주사법은 생리식염수에 융해한 FSH를 1일 50mg씩 2회(12시간 간격)주사하는 법으로 CIDR 질내 주입 9일째부터 4일간 주사하였으며, 질내에 CIDR를 주입하고 11일째되는 날에 PG(25mg, 루텔라이스, 한국)를 주사하고, 12일째는 CIDR를 제거하여 일회주사법과 주기를 맞추었다. 일회주사법은 CIDR를 질내에 주입하고 나서 9일째 되는 날에 400mg의 FSH을 생리식염수에 융해하여 일회주사를 하고 다회주사법과 같이 인공수정과 채란시간을 맞추었다. 인공수정은 황체퇴행제(PG)를 주사하고 나서 48, 72시간에 2회 인공수정을 실시하여 1주일 후 수정란을 채란하였다. 인공수정시는 반드시 GnRH(2.5mg)를 동시에 주사하였다. 채란은 인공수정 후 7일째 되는 날에 비외과적으로 채란을 실시하여 우수한 수정란은 동결을 실시하였다. 다회주사법에서 14두, 일회주사법에서 14두를 공시하여 황체수, 회수수정란수, 배반포수, 동결란수를 조사하였다. 다회주사법에서는 황체수는 8.07$\pm$6.62개, 회수정란수는 6.78$\pm$5.96개, 배반포율은 58.9%, 동결란 생산율은 52.6%의 성적을 얻었으며, 일회주사법에서 황체수는 12.07$\pm$8.07개, 회수수정란수는 10.0$\pm$9.24개, 배반포율은 27.1%, 동결란 생산율은 25.0% 수준의 결과를 얻었으나 결과에 대한 통계분석시 다회주사법과 일회주사리법 간에 유의적인 차는 인정되지 않았다(student T-test, P<0.05). 본 실험의 결과에서 나타나듯이 생리식염수를 용매로 이용한 FSH 1회 주사법이 다회주사법과 비교하였을 때에 수정란 생산효율에서 유의적인 차이가 없으므로 이 방법을 이용한 다배란유기는 노동력과 공란우에 대한 스트레스를 경감할 수 있을 것으로 사료된다.

• Journal of Korean Society of Environmental Engineers
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• v.31 no.5
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• pp.382-391
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• 2009

Atmospheric speciated mercury concentrations including total gaseous mercury (TGM) and reactive gaseous mercury (RGM) were measured in Chuncheon from March 2006 to November 2008. Average concentrations were 2.10 ${\pm}$ 1.50 ng/$m^3$ and 3.00 ${\pm}$ 3.14 pg/$m^3$ for TGM and RGM, respectively. RGM concentrations were higher during daytime than nighttime probably because of high photochemical activities. We found that RGM concentration considerably increased as ozone increased when fog occurred, indicating that ozone was the important oxidant for $Hg^0$ in aqueous phase. TGM concentration showed positive correlations with CO and $PM_{10}$ which can transport in long-range, but there was no correlation with $NO_2$. Considering that major source of mercury is combustion process, this result showed that local sources did not significantly impact on TGM concentration in Chuncheon. Five-day backward trajectories were calculated for the samples representing high and low concentrations of TGM, and determined that industrialized area of China including Shenyang and Beijing influenced TGM concentrations in Chuncheon.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.14
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• pp.5773-5777
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• 2015

Background: Oral squamous cell carcinoma (OSCC) is thought to develop from precancerous dysplastic lesions through multistep processes of carcinogenesis involving activation of oncogenes and loss of tumor suppressor genes. The human epidermal growth factor receptor 2 (Her-2/neu [erbB-2]), a cell membrane glycoprotein, is a growth factor receptor that has receptor tyrosine kinase activity. Her2/neu activation plays a central role in cell proliferation and survival. It has been shown that overexpression of Her2/neu increases the rate of cell division and growth, leading to precancerous changes. The aim of the present study was to compare the serum and salivary Her2/neu levels between cases with premalignant and malignant oral lesions. Materials and Methods: Fasting blood samples and unstimulated saliva by passive drooling were collected from three groups of healthy control (n=20), premalignant disorder (PMD) (n=20) and OSCC (n=25) subjects. The HER2 extracellular domain (HER2 ECD) levels were measured using ELISA. Results: The levels of serum Her2/neu showed no significant differences between any of the groups but on the other hand salivary Her2/neu levels were found to be significantly (p<0.05) higher when compared between control (median 68.7 pg/ml, range: 21.5 - 75.8) and OSCC (median 145.6 pg/ml, range: 45.1-191.1). A similar trend was observed when comparing between PMD (median 43.3, range: 22.1 -94.7) and OSCC with a statistical significance of p<0.05. Conclusions: Our study provided evidence of increased salivary Her2/neu in OSCC when compared to PMD and control which was not the case for serum levels. This suggests that probably Her2/neu is not highly amplified as in breast cancer so as to be reflected in serum. Since saliva is in local vicinity of the OSCC, even a mild increase might be mirrored. On the whole, this study proposes Her2/neu as marker for distinguishing premalignant and malignant conditions.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.9
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• pp.5225-5230
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• 2013

Background: There is a Th1/Th2 cytokine imbalance and expression of IL-17 in patients with brain tumours. We aimed to compare the levels of IL-17A and IL-6 in sera of glioma, meningioma and schwannoma patients as well as in healthy individuals. Materials and Methods: IL-17A and IL-6 levels were measured in sera of 38 glioma, 24 meningioma and 18 schwannoma patients for comparison with 26 healthy controls by commercial ELISA assays. Results: We observed an increase in the IL-17A in 30% of glioma patients while only 4% and 5.5% of meningioma and schwannoma patients and none of the healthy controls showed elevated IL-17A in their sera ($0.29{\pm}0.54$, $0.03{\pm}0.15$ and $0.16{\pm}0.68$ vs. $0.00{\pm}0.00pg/ml$; p=0.01, p=0.01 and p=0.001, respectively). There was also a significant decrease in the level of IL-6 in glioma patients compared to healthy controls ($2.34{\pm}4.35$ vs. $4.67{\pm}4.32pg/ml$; p=0.01). There was a direct correlation between the level of IL-17A and age in glioma patients (p=0.005). Glioma patients over 30 years of age had higher IL-17A and lower IL-6 in their sera compared to the young patients. In addition, a non-significant grade-specific inverse trend between IL-17A and IL-6 was observed in glioma patients, where high-grade gliomas had higher IL-17A and lower IL-6. Conclusions: Our data suggest a Th17 mediated inflammatory response in the pathogenesis of glioma. Moreover, tuning of IL-6 and IL-17A inflammatory cytokines occurs during progression of glioma. IL-17A may be a potential biomarker and/or immunotherapeutic target in glioma cases.

• Korean Journal of Agricultural Science
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• v.12 no.1
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• pp.55-61
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• 1985

This study was carried out to investigate reproductive traits and sex hormone levels by growth in Korean native goats. Serum levels of FSH, LH, prolactin, estradiol-$17{\beta}$ and progesterone were determined every 15 days from the 70 days of age to 190 days by radioimmunoassay. The age and weight at sexual maturity were 183.6 days and 14.3 kg; the length of estrous cycle and estrus period were 20.3 days and 36.7 hours, respectively. The gestation period, litter size and body weight at birth were 148.4 days, 1.4 head and 1.8 kg, respectively. The levels of serum LH were highest with 3.93 mIU/ml at 70 days of age, thereafter decreased gradually to 1.21 mIU/ml at 190 days of age. The concentrations of FSH in serum were below 1.25 mIU/ml throughout the experimental period. The levels of prolactin were lowest with 3.09ng/ml at 85 days of age and highest with 4.65 ng/ml at 175 days of age. The serum levels of estradiol-$17{\beta}$ were increased with age, thus highest with 7.95 pg/ml at 190 days of age. The serum progesterone concentrations were maintained low levels (below 1.0 ng/ml) throughout the experimental period.