Characterization of Two Type Crystal Proteins Produced by Transformed Bacillus thuringiensis NT0423

형질전환된 Bacillus thuringiensis NT0423 균주에 의해 생산된 두가지 형태의 내독소 단백질의 특성

  • 박현우 (서울대학교 농업생물 신소재연구센터) ;
  • 김호산 (서울대학교 농업생물 신소재연구센터) ;
  • 유용만 (서울대학교 농업생물 신소재연구센터) ;
  • 김상현 (농총진흥청 잠업시험장) ;
  • 서숙재 (경상대학교 자연과학대학생물학과) ;
  • 강석권 (서울대학교 농업생물신소재연구센터)
  • Published : 1993.10.01

Abstract

Cloning and expression of two different crystal protein genes from transformed Bacillus thuringiensis were investigated. B. thuringiensis NT0423 is toxic to both Lepidopterous and Dipte-rous larvae. The pCG5 vector carrying crystal protein genes (mosquitocidal and hemolytic activity) of B. thurigiensis subsp. morrisoni PG-14 was transformed into B. thurigiensis NT0423. Transformant has expressed two type crystals of bipyramid from NT0423 and ovoid from pCG5 in one cell.

Keywords

References

  1. Can. J. Microbiol. v.1 The protein crystals of Bacillus thuringiensis berliner Hannay,C.L.;D. Fitz-James
  2. Mosq. News v.37 A bacterial spore demonstrating rapid larvieidal activity against Anopheles serengetii. Uranotaenia unguiculata. Culex univittatus. Aedes aegypti and Culex pipiens Goldberg,L.J.;J.Margalit
  3. Z. Angrew. Entomol. v.96 Bacillus thuringiensis var. tenebrionis, a new pathotype effective against larvae of Coleoptera Krieg,V.A.;A.M.Huger;G.A.Langenbruch;W.Schnetter
  4. Arch. Biochem. Biophys. v.227 Two types of entomocidal toxins in the parasporal crystals of Bacillus thuringiensis kurstaki Yamamoto,T.;T.Iizuka
  5. FEMS Microbiol. Lett. v.60 Transformation and expression of cloned δ-endotoxin gene in Bacillus thuringiensis Lereclus,D.;O.Arantes;J.Chaufaux;M.M.Lecadet
  6. Microbiol. Rev. v.50 Bacillus thuringiensis and related insect pathogens Aronson,A.I.;W.Beckman;P.Dunn
  7. Annu. Rev. Microbiol. v.41 The molecular biology of parasporal crystal body formation in Bacillus thuringiensis Whiteley,H.R.;H,E.Schnepf
  8. Bacillus subtilis. Appl. Environ. Microbiol. v.55 Expression of a cloned Bacillus thuringiensis δ-endotoxin gene Calogero,S.;A.M.Albertini;C.Fogher;R.Marzari;A.Galizzi
  9. Appl. Environ. Microbiol. v.56 A translation fusion product of two different insecticidal crystal protein genes of Bacillus thuringiensis exhibits an enlarged insecticidal spectrum Honee,G.;W.Vriezen;B.Visser
  10. Plant Physiol. v.85 Bacillus thuringiensis δ-endotoxin expressed in transgenic Nicotiana tabacum provides resistance to lepidopteran insects Barton,K.A.;H.R.Whiteley;N.S.Yang
  11. Nature(London) v.328 Transgenic plants protected from insect attack Vaeck,M.;A.Reynaerts;H.H$\ "{o} $fte;S.Jansens;M.De Beuckeleer;C.Dean;M.Zabeau;M.Van Montagu;J.Leemans
  12. FEMS Microbiol. Lett. v.60 Transformation of Bacillus thuringiensis by electroporation Mahilon,J.;W.Chungjatupornchai;J.Decock;S.Dierickx;F.Michiels;M.Peferoen;H.Joos
  13. Bio/Technology v.10 Expansion of insecticidal host range of Bacillus thuringiensis by in vivo genetic recombination Lereclus,D.;M.Vallade;J.Chaufaux;O.Arantes;S.Rambaud
  14. Appl. Environ. Microbiol. v.58 Construction of novel Bacillus thuringiensis strains with different insecticidal activities by transduction and transformation Lecadet,M.M.;J.Chaufaux;J.Ribier;D.Leeclus
  15. K. J. Appl. Entomol. Characterization of activity crystal protein produced by new Bacillus thuringiensis NT0423 isolated from soil Kim,H.S.;H.W.Park;Y.M.Yu;S.K.Kang
  16. Appl. Environ. Microbiol. v.59 High level cryIVD and cytA gene expression in Bacillus thuringiensis does not require the 20-kilodalton protein, and the coexpressed gene products are synergistic in their toxicity to modquitoes Chang,C.;Y.M.Yu;S.M.Dai;S.K.Law;S.S.Gill
  17. Mol. Gen. Genet. v.218 Efficient transformation of Bacillus thuringiensis and Bacillus cerus via electroporation:transformation of a crystalliferous strains with a cloned delta-endotocon genes Schurter,W.;M.Geiser;D.Mathe
  18. Nucleic Acid Res. v.7 A rapid alkaline extraction procedure for screening recombinant plasmid DNA Birnboim,H.C.;J.Doly
  19. J. Cell Sci. v.60 Bacillus thuringiensis var, israelensis crystal δ-endotoxin:Effects on insect and mammalian cells in vitro and in vivo Thomas,W.E.;D.J.Ellar
  20. Nature(London) v.227 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Laemmli,U.K.
  21. Appl. Environ. Microbiol. v.35 Transduction in Bacillus thuringiensis Thorne,C.B.
  22. Proc. Natl. Acad. Sci. USA v.79 Transfer of Bacillus thuringiensis plasmids coding for δ-endotoxin among strains of Bacillus thuringiensis and Bacillus cereus Gonzalez,J.M.Jr.;B.J.Brown;B.C.Carlton
  23. Gene v.45 Integration of the delta-endotoxin gene of Bacillus thuringiensis into the chromosome of root-colonizing strains of Pseudomonds using Tn5 Obukowitez,M.G.;F.J.Perlak;F.Kusano-Kretzmer;E.J.Mayer;L.S.Wartrud
  24. Appl. Environ. Microbiol. v.58 Cloning and expression of the cryIVD gene of Bacillus thuringiensis subsp. israelensis in the cyanobacterium Agmenellum quadruplicatum PR-6 and its resulting larvicidal activity Murphy,R.C.;S.E.Stevens,Jr.
  25. Science v.229 Insect resistance to the biological insecticide Bacillus thuringiensis McGaughey,W.H.
  26. FEMS Microbiol. Lett. v.58 Transformation of Bacillus thuringiensis by electroporation Bone,E.J.;D.J.Ellar
  27. FEBS Lett. v.190 Synergism in mosquitocidal activity of 26 and 65 kDa proteins from Bacillus thuringiensis subsp. israelensis crystal Wu,D.;F.N.Chang
  28. J. Gen. Appl. Microbiol. v.33 Synergistic effects of the 65- and 25-kilodalton proteins of Bacillus thuringiensis strain PG-14(Serotype 8a:8b) in mosquito larvieidal activity Yu,Y.M.;M.Ohba;K.Aizawa
  29. Microbiol. Rev. v.53 Insecticidal crystal proteins of Bacillus thuringiensis Hofte,H.;H.R.Whiteley
  30. Mol. Gen. Genet. v.186 Molecular relationships among the plasmids of Bacillus thuringiensis: conserved sequences through 11 crystalliferous strains Lereclus,D.;M.M.Lecadet;J.Ribier;R.Dedoner
  31. J. Bacteriol. v.172 Identification of δ-exotoxin production, plasmids encoding δ-exotoxin, and a new exotoxin in Bacillus thuringiensis by using high-performance liquid chromatography Levinson,B.L.;K.J.Kasyan;S.S.Chiu;T.C.Currier;J.M.Jr.,Gonzalez