Journal of Species Research

The National Institute of Biological Resources (국립생물자원관)
권호 표지
DOI QR코드

DOI QR Code

Report of two unrecorded yeast species in the class Tremellomycetes

  • Seonjae Kim (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Sathiyaraj Srinivasan (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University)
  • Received : 2023.11.15
  • Accepted : 2023.12.14
  • Published : 2024.05.31
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study is to isolate and identify wild yeasts from the soil samples collected in Daegu and Daejeon City, Republic of Korea. Among 15 strains isolated in this study, 13 strains were previously reported and two strains had not been reported in Republic of Korea. To identify wild yeast strains, pairwise sequence comparisons of D1/D2 region of the 26S rRNA gene sequence were done using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and assimilation tests were done using API 20C AUX kit. All strains were assigned to the phylum Basidiomycota. The two unrecorded yeast strains, PG2-2-10C and DJ2-14-10C, belong to the genus Holtermanniella (family Holtermanniaceae, order Holtermanniales, class Tremellomycetes) and Goffeauzyma (family Filobasidiaceae, order Filobasidiales, class Tremellomycetes), respectively. The two unrecorded yeast strains had oval shape and polar budding cells. This research describers the morphological and biochemical properties of the two unreported yeast species that had not officially reported in Korea.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (No.2021R1F1A1061389), also supported by National Institute of Biological Resources (NIBR) (2022M3H9A1082984) and a grant from the National Institute of Biological Resources (NIBR) funded by the Ministry of Environment(MOE) of the Republic of Korea (NIBR202304104).

References

  1. Altschul, S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller and D.J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25(17):3389-3402. https://doi.org/10.1093/nar/25.17.3389
  2. Fell, J.W., T. Boekhout, A. Fonseca, G. Scorzetti and A. Statzell-Tallman. 2000. Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 50(3):1351-1371. https://doi.org/10.1099/00207713-50-3-1351
  3. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39(4):783-791. https://doi.org/10.2307/2408678
  4. Fonseca, A., T. Boekhout and J.W. Fell. 2011a. The yeasts: a taxonomic study. In: Kurtzman, C.P., J.W. Fell, T. Boekhout(eds.). Elsevier, Amsterdam, pp. 1661-1737.
  5. Fonseca, A., T. Boekhout, and J.W. Fell. 2011b. The yeasts: a taxonomic study. In: Kurtzman, C.P., J.W. Fell, T. Boekhout(eds.). Elsevier, Amsterdam, pp. 1591-1594.
  6. Golubev, W.I., J.P. Sampaio, L. Alves and N.W. Golubev. 2004. Cryptococcus festucosus sp. nov. a new hymenomycetous yeast in the Holtermannia clade. Canadian Journal of Microbiology 50(12):1001-1006. https://doi.org/10.1139/w04-099
  7. Guffogg, S.P., S. Thomas-Hall, P. Holloway and K. Watson. 2004. A novel psychrotolerant member of the hymenomycetous yeasts from Antarctica: Cryptococcus watticus sp. nov. Int J Syst Evol Microbiol 54:275-277. https://doi.org/10.1099/ijs.0.02877-0
  8. Kimura, M. 1983. The neutral theory of molecular evolution. Cambridge University Press.
  9. Kurtzman, C.P. and C.J. Robnett. 1998. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit(26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73(4):331-371. https://doi.org/10.1023/A:1001761008817
  10. Libkind, D., M.T. Arts and M. Van Broock. 2008. Fatty acid composition of cold-adapted carotenogenic basidiomycetous yeasts. Rev Argent Microbiol 40:193-197.
  11. Liu, X.Z., Q.M. Wang, M. Goker, M. Groenewald, A.V. Kachalkin, H.T. Lumbsch, A.M. Millanes, M. Wedin, A.M. Yurkov, T. Boekhout and F.Y. Bai. 2015. Towards an integrated phylogenetic classification of the Tremellomycetes. Stud Mycol 81:85-147. https://doi.org/10.1016/j.simyco.2015.12.001
  12. McMurrough, I. and A.H. Rose. 1973. Effects of temperature variation on the fatty acid composition of a psychrophilic Candida species. J Bacteriol 114:451-452. https://doi.org/10.1128/jb.114.1.451-452.1973
  13. Reiersol, S. and M. di Menna. 1958. A new Cryptococcus species. Antonie van Leeuwenhoek 24:27-30. https://doi.org/10.1007/BF02548428
  14. Rossi, M., P. Buzzini, L. Cordisco, A. Amaretti, M. Sala, S. Raimondi, C. Ponzoni, U.M. Pagnoni and D. Matteuzzi. 2009. Growth, lipid accumulation, and fatty acid composition in obligate psychrophilic, facultative psychrophilic, and mesophilic yeasts. FEMS Microbiol Ecol 69:363-372. https://doi.org/10.1111/j.1574-6941.2009.00727.x
  15. Russo, G., D. Libkind, R.J. Ulloa, V. de Garcia, J.P. Sampaio and M.R. van Broock. 2010. Cryptococcus agrionensis sp. nov., a basidiomycetous yeast of the acidic rock drainage ecoclade, isolated from an acidic aquatic environment of volcanic origin. Int J Syst Evol Microbiol 60(4):996-1000. https://doi.org/10.1099/ijs.0.012534-0
  16. Sampaio, J.P. 2011a. Leucosporidiella Sampaio. 2003. In: Kurtzman, C.P., J.W. Fell, T. Boekhout (eds.). The yeasts, a taxonomic study, vol 3, 5th edn. Elsevier, New York, pp. 1801-1806.
  17. Sampaio, J.P. 2011b. Leucosporidium Fell, Statzell, Hunter and Phaff (1969). In: Kurtzman, C.P., J.W. Fell, T. Boekhout(eds.). The yeasts, a taxonomic study, vol 3, Elsevier, New York, pp. 1485-1494.
  18. Tamura, K., G. Stecher and S. Kumar. 2021. MEGA11: molecular evolutionary genetics analysis version 11. Mol Biol Evol 38(7):3022-3027. https://doi.org/10.1093/molbev/msab120
  19. Trees, R.P. 1987. The Neighbor-joining Method: A New Method for. Mol. Biol. Evol 4(4):406-425.
  20. Wuczkowski, M., V. Passoth, B. Turchetti, A.C. Andersson, M. Olstorpe, A. Laitila, B. Theelen, M. van Broock, P. Buzzini, H. Prillinger, K. Sterflinger, J. Schnurer, T. Boekhout and D. Libkind. 2011. Description of Holtermanniella gen. nov., including Holtermanniella takashimae sp. nov. and four new combinations, and proposal of the order Holtermanniales to accommodate tremellomycetous yeasts of the Holtermannia clade. Int J Syst Evol Microbiol 61(3):680-689. https://doi.org/10.1099/ijs.0.019737-0