• BMB Reports
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• v.50 no.5
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• pp.275-280
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• 2017

Herpes simplex virus type 1 ICP27 is a multifunctional protein responsible for viral replication, late gene expression, and reactivation from latency. ICP27 interacts with various cellular proteins, including Daxx. However, the role of interaction between ICP27 and Daxx is largely unknown. Since Daxx is known to repress $NF-{\kappa}B$ activity, there is a possibility that ICP27 may influence the inhibitory effect of Daxx on $NF-{\kappa}B$ activity. In this study, we tested whether ICP27 affects the $NF-{\kappa}B$ activity through its interaction with Daxx. Interestingly, ICP27 enhanced the Daxx-mediated repression of $NF-{\kappa}B$ activity. In addition, we found that sumoylation of Daxx regulates its interaction with p65. ICP27 binds to Daxx, inhibits Daxx sumoylation, and enhances p65 deacetylation induced by Daxx. Consequently, ICP27 represses the $NF-{\kappa}B$ activity, by elevating the inhibitory effect of Daxx on $NF-{\kappa}B$ activity through desumoylation of Daxx.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.4
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• pp.821-828
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• 2008

Onchungeum, a herbal formula, which has been used for treatment of anemia due to bleeding, discharging blood and skin disease. In the present study, it was examined the effects of extract of Onchungeum (OCE) on the growth of human hepatocarcinoma cell lines Hep3B (p53 null type) and HepG2 (p53 wild type) in order to investigate the anti-proliferative mechanism by OCE. Treatment of Hep3B and HepG2 cells to OCE resulted in the growth inhibition in a dose-dependent manner, however Hep3B cell line exhibited a relatively strong anti-proliferative activity to OEC. Flow cytometric analysis revealed that OCE treatment in Hep3B cells caused G1 phase arrest of the cell cycle, which was associated with various morphological changes in a dose-dependent fashion. RT-PCR and immunoblotting data revealed that treatment of OCE caused the down-regulation of cyclin D1 expression, however the levels of cyclin E expression were not changed by OCE. The G1 arrest of the cell cycle was also associated with the induction of Cdk inhibitor p27 by OCE. Because the p53 gene is null in Hep3B cells, it is most likely that the induction of p21 is mediated through a p53-independent pathway. Moreover, p27 detected in anti-Cdk4 and anti-Cdk2 immunoprecipitates from the OCE-treated cells, suggesting that OCE-induced p27 protein blocks Cdk kinase activities by directing binding to the cyclin/Cdk complexes. Furthermore, OCE treatment potently suppresses the phosphorylation of retinoblastoma proteins and the levels of the transcription factor E2F-1 expression. Taken together, these results indicated that the growth inhibitory effect of OCE in Hep3B hepatoma cells was associated with the induction of G1 arrest of the cell cycle through regulation of several major growth regulatory gene products.

• Journal of Physiology & Pathology in Korean Medicine
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• v.16 no.4
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• pp.745-755
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• 2002

To examine the effects of Yunpyesan on the cell proliferation of A549 human lung carcinoma cell line, we performed various experiments such as dose-dependent effect of Yunpyesan on cell proliferation and viability, morphological changes, quantification of apoptotic cell death and alterations of apoptosis/cell cycle-regulatory gene products. Yunpyesan declined cell viability and proliferation in both a dose- and a time-dependent manner. The anti-proliferative effect by Yunpyesan treatment in A459 cells was associated with morphological changes such as membrane shrinking and cell rounding up. Yunpyesan Induced apoptotic cell death in a time-dependent manner, which was associated with degradation of poly-(ADP-ribose) polymerase (PARP), an apoptotic target protein, without alterations of the balance between Bcl-2 and Bax expressions. DNA flow cytometric histograms showed that population of G1 phase of the cell cycle was increased by Yunpyesan treatment in a dose-dependent manner. Western blot analysis revealed that cyclin D1 and A were reduced by Yunpyesan treatment, whereas cyclin dependent kinase (Cdk) inhibitor p27 was markedly increased in a time-dependent fashion. The level of tumor suppressor p53 proteins was also increased by Yunpyesan treatment and its increase might be linked to increase of Cdk inhibitor p27. In addition, Mdm2, negative regulator of p53, was down-regulated by Yunpyesan treatment. Since the expression of retinoblastome protein (pRB), a key regulator of G1/S progression, was reduced by Yunpyesan treatment, we supposed that phosphorylation of pRB might be also blocked. The present results indicated that Yunpyesan-induced inhibition of lung cancer cell proliferation is associated with the induction of apoptosis and the blockage of G1/S progression.

• Journal of the Korean Society of School Health
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• v.27 no.2
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• pp.90-99
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• 2014

Purpose: This study examined the levels of and the associations between middle school students' attachments to parents, peers, and teachers, truancy, and school bullying. Methods: Data on middle school student were obtained from the Korea National Children and Youth Survey 2010 (N=2,968). The independent t-test and Kruskal-Wallis test were conducted to examine the differences in truancy and school bullying according to the characteristics of students. Multiple stepwise regression analysis was conducted to identify the factors influencing school bullying. Results: The magnitude of the correlation between truancy and bullying was r=.34 (p<.001). Two types of attachment(to parents and to teachers) were associated with school bullying, with the magnitude of r=-.06 (p=.003) and r=-.09 (p<.001), respectively. Truancy and all three types of attachment (to parents, peers, and teachers) predicted school bullying significantly (F=27.84, p<.001). Conclusion: In this study, truancy had more influence on bullying behavior than attachments to parents, teachers and peers among middle school students. These results suggest there is need to develop and provide programs to prevent or early intervene in truancy, an important predictor of school bullying, to reduce school bullying among middle school students.

• Journal of East-West Nursing Research
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• v.27 no.1
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• pp.32-42
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• 2021

Purpose: The purpose of this study was to identify factors influencing nursing performance of clinical nurses. Methods: This was a descriptive study involving 172 nurses who worked for 6 months or longer at one general hospital in B City. The data analysis was conducted with t-test, ANOVA, Scheffé test, Pearson's correlation coefficients, and stepwise multiple regression. Results: Nursing performance of participants positively correlated with job stress (r=.16, p=.033). The factors affecting nursing performance were total clinical experience (β=.37 p<.001), salary (β=.19, p=.028), and job stress (β=.13, p=.045). These variables had a 27.2% variance to explain nursing performance. Conclusion: Based on these results, administrative and financial support are required for the development of a clinical career program, including the differentiation of salary systems of clinical nurses. There is also a need to develop and implement job stress management and coping programs.

• Journal of IKEEE
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• v.22 no.1
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• pp.209-212
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• 2018

In this work, we developed P(VDF-TrFE) organic/ferroelectric material based metal-ferroelectric-metal (MFM) capacitors in order to improve the switching characteristics of gallium nitride (GaN) heterojunction field-effect transistors (HFET). The 27 nm-thick P(VDF-TrFE) MFM capacitors exhibited about 60 ~ 96 pF capacitance with a polarization density of $6{\mu}C/cm^2$ at 4 MV/cm. When the MFM capacitor was connected in series with the gate electrode of GaN HFET, the subthreshold slope decreased from 104 to 82 mV/dec.

• Journal of East-West Nursing Research
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• v.27 no.1
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• pp.61-69
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• 2021

Purpose: This study aims to determine the association between fear of falling, falls efficacy, and fall prevention behaviors in older adults in outpatient and identify the factors affecting fall prevention behaviors. Methods: This was a descriptive study. A total of 137 older adults aged 60 or older with more than one disease in an outpatients setting were recruited from a university hospital in Seoul. Data were collected from March 11 through April 8, 2016. Results: The experience of a fall was significantly associated with age (t=-2.71, p=.008), gender (𝛘²=14.32, p<.001), education (𝛘²=6.05, p=.049), income (𝛘²=9.93, p=.019), physical activity status (𝛘²=16.84, p<.001), and the number of diseases (t=-2.89, p=.004). Fall experience was not significantly associated with marital status (𝛘²=3.79, p=.285). Multiple regression analysis revealed that the most influential factor of fall prevention behaviors was the fear of falls (β=-.37, p<.001), followed by the number of diseases (β=-.20, p=.013). Conclusion: It is necessary to provide preventive fall education with older adults in outpatients settings considering the fear level of falling and the numbers of accompanying diseases.

• Journal of the Korean Society of Industry Convergence
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• v.27 no.2_2
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• pp.307-318
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• 2024

This study conducted to examine the effects of a GMO education(n=108 university students). Although the experience of encountering GMO was 84.3%, awareness was moderate or lower for 76.9%, 62% were unaware of GMO labeling, and 44.4% had no previous education on GMO. After the education, GMO positive perceptions, specifically in terms of improving work efficiency(p<0.05), negative perception(p<0.001), and GMO knowledge increased significantly(p<0.05). The need for expanding GMO labeling (p<0.05) and adjust to 0.9%(exclusion criteria) for labeling(p<0.001) increased significantly. Moreover, there was a significant increase in the need and willingness to accept a price increase for the full labeling system(p<0.01), with a significant increase in the willingness to pay up to 20%(p<0.05). The pre-contemplation stage showed a significant decrease(p<0.001). The preparation and behavior and maintenance stage showed a significant increase(p<0.001). Based on these findings, it is suggested that GMO education be implemented to provide accurate information and emphasize the necessity of consumers' right to know and choose through the implementation of the full labeling system of GM foods.

• Communications of the Korean Mathematical Society
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• v.13 no.1
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• pp.21-27
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• 1998

In this paper we prove a structure theorem for p-hyponomal contractions and also give an example of a p-hyponormal operator which is not *-paranormal.

• Food Science of Animal Resources
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• v.26 no.1
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• pp.127-135
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• 2006

This experiment was carried out to examine the fermentation properties of yogurt with bovine milk and soybean milk at the mixed ratio of 2:1 and added 0.1, 0.2, 0.4 and 1.0% red ginseng extract. The effect on promoting the fermentation by additives 0.1, 0.2, 0.4 and 1.0% red ginseng extracts were higher and pH was $3.90{\sim}3.94$ when Lactobacillus acidophilus KCTC3150 and Lactobacillus salivarius ssp. salivarius CNU27 were used. Titratable acidity showed a little inhibiting due to increasing red ginseng extract content. The average viable counts of lactic acid bacteria after 15 hour culture was the highest level of $6.26{\times}10^8cfu/mL$ when Lactobacillus acidophilus KCTC3150 was used, and the additives content of red ginseng extract was 1.0% The production of lactic acid was the highest and the concentration was 332.22 mM when Lactobacillus acidophilus KCTC3150 was used, and the additives content of red ginseng extracts was 1.0% Lactose hydrolysis was completely hydrolyzed when Lactobacillus acidophilus KCTC3150 and Lactobacillus salivarius ssp. salivarius CNU27 were used. The highest viscosity of yogurt was 780 cP when Lactobacillus acidophilus KCTC3150 and Lactobacillus salivarius subsp. salivarius CNU27 were used and red ginseng extract was added 1.0% The overall acceptability, $4.17{\pm}0.64$, was the highest when Lactobacillus salivarius subsp. salivarius CNU27 was used and the additives content of red ginseng extract was 0.2%.