• Title/Summary/Keyword: Organ fabrication

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Fabrication of Calcium Phosphate Scaffolds Using Projection-based Microstereolithography and Their Effects on Osteogenesis (투영기반 마이크로 광조형 기술을 이용한 3 차원 인산칼슘 인공지지체 제작 및 골 분화 영향)

  • Seol, Young-Joon;Park, Ju-Young;Cho, Dong-Woo
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.35 no.11
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    • pp.1237-1242
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    • 2011
  • Calcium phosphates are very interesting materials for use as scaffolds for bone tissue engineering. These materials include hydroxyapatite (HA) and tricalcium phosphate (TCP), which are inorganic components of human bone tissue and are both biocompatible and osteoconductive. Although these materials have excellent properties for use as bone scaffolds, many researchers have used these materials as additives to synthetic polymer scaffolds for bone tissue regeneration, because they are difficult to manufacture three-dimensional (3D) scaffolds. In this study, we fabricated 3D calcium phosphate scaffolds with the desired inner and outer architectures using solid freeform fabrication technology. To fabricate the scaffold, the sintering behavior was evaluated for various sintering temperatures and slurry concentrations. After the fabrication of the calcium phosphate scaffolds, in-vitro cell proliferation and osteogenic differentiation tests were carried out.

Three-Dimensional Printed 3D Structure for Tissue Engineering (3 차원 프린팅 기술로 제작된 조직공학용 3 차원 구조체)

  • Park, Jeong Hun;Jang, Jinah;Cho, Dong-Woo
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.38 no.10
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    • pp.817-829
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    • 2014
  • One of the main issues in tissue engineering has been the development of a three-dimensional (3D) structure, which is a temporary template that provides the structural support and microenvironment necessary for cell growth and differentiation into the target tissue. In tissue engineering, various biomaterials and their processing techniques have been applied for the fabrication of 3D structures. In particular, 3D printing technology enables the fabrication of a complex inner/outer architecture using a computer-aided design and manufacturing (CAD/CAM) system, and it has been widely applied to the fabrication of 3D structures for tissue engineering. Novel cell/organ printing techniques based on 3D printing have also been developed for the fabrication of a biomimetic structure with various cells and biomaterials. This paper presents a comprehensive review of the functional scaffold and cell-printed structures based on 3D printing technology and the application of this technology to various kinds of tissues regeneration.

The Design and Fabrication of μCCA-μGI Device for Toxicity Evaluation of Acetaminophen (아세트아미노펜 독성평가를 위한 μCCA-μGI 디바이스의 개발)

  • Chang Jung-Yun;Shuler Michael L.
    • Journal of Pharmaceutical Investigation
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    • v.36 no.4
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    • pp.263-269
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    • 2006
  • Deficiencies in the early ADMET(absorption, distribution, metabolism, elimination and toxicity) information on drug candidate extract a significant economic penalty on pharmaceutical firms. Microscale cell culture analogue-microscale gastrointestinal(${\mu}CCA-{\mu}GI$) device using Caco 2, L2 and HEp G2/C3A cells, which mimic metabolic process after absorption occurring in humans was used to investigate the toxicity of the model chemical, acetaminophen(AAP). The toxicity of acetaminophen determined after induction of CYP 1A1/2 in Caco 2 cells was not significant. In a coculture system, although no significant reduction in viability of HEp G2/C3A and L2 cells was found, approximately 5 fold increase in the CYP 1A1/2 activity was observed. These results appear to be related to organ-organ interaction. The oral administration of a drug requires addition of the absorption process through small intestine to the current ${\mu}CCA$ device. Therefore, a perfusion coculture system was employed for the evaluation of the absolution across the small intestine and resulting toxicity in the liver and lung. This system give comprehensive and physiologic information on oral uptake and resulting toxicity as in the body. The current ${\mu}CCA$ device can be used to demonstrate the toxic effect due to organ to organ interaction after oral administration,

A Case Study on Precise NURBS Modeling of Human Organs (인체장기의 정밀한 NURBS 곡면 모델링 사례연구)

  • Kim H.C.;Bae Y.H.;Soe T.W.;Lee S.H.
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2005.06a
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    • pp.915-918
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    • 2005
  • Advances in Information Technology and in Biomedicine have created new uses for CAD technology with many novel and important biomedical applications. Such applications can be found, for example, in the design and modeling of orthopedics, medical implants, and tissue modeling in which CAD can be used to describe the morphology, heterogeneity, and organizational structure of tissue and anatomy. CAD has also played an important role in computer-aided tissue engineering for biomimetic design, analysis, simulation and freeform fabrication of tissue scaffolds and substitutes. And all the applications require precision geometry of the organs or bones of each patient. But the geometry information currently used is polygon model with none solid geometry and is so rough that it cannot be utilized for accurate analysis, simulation and fabrication. Therefore a case study is performed to deduce a transformation method to build free form surface from a rough polygon data or medical images currently used in the application. This paper describes the transformation procedure in detail and the considerations for accurate organ modeling are discussed.

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Development of Scaffold Fabrication System using Multi-axis RP Software Technique (다축 RP 소프트웨어 기술을 이용한 스캐폴드 제조 장비 개발)

  • Park, Jung-Whan;Lee, Jun-Hee;Cho, Hyeon-Uk;Lee, Su-Hee;Park, Su-A;Kim, Wan-Doo
    • Journal of the Korean Society for Precision Engineering
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    • v.29 no.1
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    • pp.33-40
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    • 2012
  • The scaffold serves as 3D substrate for the cells adhesion and mechanical support for the newly grown tissue by maintaining the 3D structure for the regeneration of tissue and organ. In this paper, we proposed integrated scaffold fabrication system using multi-axis rapid prototyping (RP) technology. It can fabricate various types of scaffolds: arbitrary sculptured shape, primitive shape, and tube shape scaffolds by layered dispensing biocompatible/ biodegradable polymer strands in designated patterns. In order to fabricate the 3D scaffold, we need to generate the plotting path way for the scaffold fabrication system. We design a data processing program - scaffold plotting software, which can convert the 3D STL file, primitive and tube model images into the NC code for the system. Finally, we fabricated the customized 3D scaffolds with high accuracy using the plotting software and the fabrication system.

A Review of the Fabrication of Soft Structures with Three-dimensional Printing Technology (3차원 프린팅 기술을 이용한 연성 구조물 제작)

  • Jang, Jinah;Cho, Dong-Woo
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.14 no.6
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    • pp.142-148
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    • 2015
  • 3D printing technology is a promising technique for fabricating complex 3D architectures based on the CAD/CAM system, and it has been extensively investigated to manufacture structures in the fields of mechanical engineering, space technology, automobiles, and biomedical and electrical applications. Recent advances in the 3D printing of soft structures have received attention for the application of the construction of flexible sensors of soft robotics or the recreation of tissue/organ-specific microenvironments. In this review paper, we would like to focus on delivering state-of-the-art fabrication of soft structures with 3D printing technology and its various applications.

Assessment of Hemodynamic Properties of Trileaflet Polymer Heart Valve Manufactured By Vacuum Forming Process (진공성형을 이용한 삼엽식 고분자 심장판막의 제작과 혈류역학적 성능평가)

  • Kim, K.H.;Hwang, C.M.;Jeong, G.S.;Ahn, C.B.;Kim, B.S.;Lee, J.J.;Nam, K.W.;Sun, K.
    • Journal of Biomedical Engineering Research
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    • v.27 no.6
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    • pp.418-426
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    • 2006
  • In the artificial heart application, productivity and hemodynamic properties of artificial heart valves are crucial in successiful application to long term in vivo trials. This paper is about manufacture and assessment of trileaflet polymer heart valves using vacuum forming process(VFP). The VFP has many advantages such as reduced fabrication time, reproducibility due to relatively easy and simple process for manufacturing. Prior to VFP of trileaflet polymer heart valves, polyurethane(Pellethane 2363 80AE, Dow Chemical) sheet was prepared by extrusion. The sheets were heated and formed to mold shape by vacuum pressure. The vacuum formed trileaflet polymer heart valves fabrication is composed of two step method, first, leaflet forming and second, conduit forming. This two-step forming process made the leaflet-conduit bonding stable with any organic solvents. Hydrodynamic properties and hemocompatibility of the vacuum formed trileaflet polymer heart valves was compared with sorin bicarbon bileaflet heart valve. The percent effective orifice area of vacuum formed trileaflet polymer heart valves was inferior to bileaflet heart valve, but the increase of plasma free hemoglobin level which reflect blood damage was superior in vacuum formed trileaflet polymer heart valves Vacuum formed trileaflet polymer heart valves has high productivity, and superior hemodynamic property than bileaflet heart valves. Low manufacturing cost and blood compatible trileaflet polymer heart valves shows the advantages of vacuum forming process, and these results give feasibility in in vivo animal trials in near future, and the clinical artificial heart development program.

3D Bioprinting Technology in Biochemical Engineering (바이오화학공학에서 3D 바이오프린팅 기술)

  • Eom, Tae Yoon
    • Korean Chemical Engineering Research
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    • v.54 no.3
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    • pp.285-292
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    • 2016
  • Three-dimensional (3D) printing is driving major innovation in various areas including engineering, manufacturing, art, education and biosciences such as biochemical engineering, tissue engineering and regenerative medicine. Recent advances have enabled 3D printing of biocompatible materials, cells and supporting components into complex 3D functional tissues. Compared with non-biological printing, 3D bioprinting involves additional complexities which require the integration of technologies from the fields of biochemical engineering, biomaterial sciences, cell biology, physics, pharmaceutics and medical science.

An Overview of Laser-assisted Bioprinting (LAB) in Tissue Engineering Applications

  • Ventura, Reiza Dolendo
    • Medical Lasers
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    • v.10 no.2
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    • pp.76-81
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    • 2021
  • Biological tissues and organs are composed of different arrays of cells, biochemical cues, and extracellular matrices arranged in a complex microarchitecture. Laser-Assisted Bioprinting (LAB) is an emerging and promising technology that is reproducible with high accuracy that can be used for fabricating complex bioengineered scaffolds that mimic tissues and organs. The LAB process allows researchers to print intricate structural scaffolds using cells and different biomaterials essential for facilitating cell-scaffold interaction and to induce tissue and organ regeneration which cannot be achieved in a traditional scaffold fabrication. This process can fabricate artificial cell niches or architecture without affecting cellular viability and material integrity. This review tackles the basic principles and key aspects of Laser-Assisted Bioprinting. Recent advances, limitations, and future perspectives are also discussed.

Functional Nanochannels to Control Ion Transportation with Monomolecule Selectivity (단일 이온 인식형 이송 제어 기능성 나노채널 기술)

  • Kim, Jeong Hwan;Lee, Eung-Sug;Whang, Kyung-Hyun;Yoo, Yeong-Eun;Yoon, Jae-Sung
    • Transactions of the KSME C: Technology and Education
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    • v.3 no.4
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    • pp.249-255
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    • 2015
  • Functional nanochannels were fabricated in order to control selective ion transportation with high permeability and low energy consumption. In this research, nanochannel platform fabrication process and surface functionalization process were developed. In addition, selective ion transportation and concentration measurement system was also set-up. By using fabricated multilayer metal membrane with electrical bias, 95% of ion ($Cl^-$) was blocked. This developed process is new-conceptional membrane fabrication technology and is expected to be applied to next-generation water purification/desalination, portable artifical kidney, and artificial sense organ.