• Korean Journal of Environment and Ecology
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• v.33 no.1
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• pp.86-106
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• 2019

We investigated the environmental conditions of natural habitats of T. nuda. The species was found on rocky northern hills ($60{\sim}90^{\circ}$) near the stream where the sea level ranges 95~145m. The average annual temperature of the habitats was lower than other places of South Korea. The differences of the lowest and the highest of the year was significantly huge than any other places. Plants were growing at the edge of stream that water reached but not submerged. Most of plants were found in North, Northeast or Northwest. It is suggested that these species require moist and low sunlight for growth. The common vegetation along with the T. nuda includes Mukdenia rossii, Selaginella rossii, Calamagrostis epigeios, and Rhododendron yedoense f. poukhanense. The dominance values and sociability of T. nuda were below 3 in all studied habitats and the variance of the number of individuals among the habitats was very high. As the optimum habitats for the T. nuda are decreasing due to the extreme precipitation patterns. It is also expected that the number of T. nuda will be decreased in the future. Therefore restoration activity in situ or ex situ must be conducted to conserve this valuable plant species.

• Clean Technology
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• v.27 no.3
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• pp.240-246
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• 2021

The waste etching solution for chip on film (COF) contained about 3.5% copper, and it was recovered through cementation using iron samples. The effect of cementation with plate, chip, and powder iron samples was investigated. The molar ratio (m/r) of iron to copper was used as a variable in order to increase the recovery rate of copper. As the molar ratio increased, the copper content in the solution rapidly decreased at the beginning of the cementation reaction. Before and after the reaction, the copper content of the solution was determined by Inductively Coupled Plasma (ICP) using copper concentration according to time. After cementation at room temperature for 1 hour, the recovery rate of copper had increased the most in the iron powder sample, having the largest specific surface area of the samples, followed by the chip and plate samples. The recovered copper powder was characterized for its crystalline phase, morphology, and elemental composition by X-ray diffraction (XRD), scanning electron microscopy (SEM), and Energy-dispersive X-ray spectroscopy (EDS), respectively. Copper and unreacted iron were present together in the iron powder samples. The optimum condition for recovering copper was obtained using iron chips with a molar ratio of iron to copper of 4 giving a recovery rate of about 98.4%.

• The Korean Journal of Mycology
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• v.48 no.4
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• pp.397-405
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• 2020

Beech mushroom (Hypsizygus marmoreus) is one of the most popular mushrooms in Japan, China, and Korea because of its delicious taste, nutritious value, and unique crunchy texture. In 2018-2019, unusual signs on the mushroom were observed in mushroom farms in Gyeongnam Province. The main signs were the inhibition of mycelial growth and primordial formation on the surface of mushroom media. When green mold caused contamination at early stage, the rate of pinhead formation by the mushroom greatly decreased to 46.5-71.5%. Conidia of the causal green mold were ellipsoidal, dark green, and measured 2.0-3.1㎛ long. The optimum temperature for mycelial growth of the green mold was 25℃. The phylogenetic tree obtained from the internal transcribed spacer (ITS) rDNA sequences showed that the isolated green mold corresponded to Penicillium brevicompactum (100.0%). This is the first report of green mold contamination caused by P. brevicompactum on H. marmoreus.

• Journal of Mushroom
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• v.19 no.2
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• pp.96-102
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• 2021

A new cultivar of oak mushroom 'Dadam' was bred from monokaryotic strains of 'GMLE36062-4' and 'GMLE36288-34'. The optimum temperature for mycelial growth of the new cultivar 'Dadam' on potato dextrose agar was 19-22℃. Total cultivation period of the new cultivar, from innoculation to its first harvest, was 135-139 days, similar to that of the control cultivar 'Hwadam'. The pileus color and stipe thickness of the new cultivar were darker and thinner than those of 'Hwadam'. Total yield of 'Dadam' was 621 g per 3 kg substrate, and is higher than that of 'Hwadam' (371 g). In farmhouse field test, it showed that the period of mycelial growth, browning, and fruiting body formation were the same as those of the control cultivar L808. The number of available fruiting bodies of 'Dadam' was 15, and is lower than that of the control (47), therefore, it was possible to save workforce in thinning. The total yield for 2 flush was 480 g for 'Dadam', similar to the 473 g of the control cultivar.

• Microbiology and Biotechnology Letters
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• v.48 no.4
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• pp.463-470
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• 2020

The JS18 strain was isolated from an old tree forest and produced extracellular enzymes that decolorize synthetic melanin. Phylogenetic analysis, based on the internal transcribed spacer (ITS) sequence, indicate that JS18 belongs to the Trametes velutina species. JS18 demonstrated laccase activity but no manganese peroxidase or lignin peroxidase activity. Batch culture indicated that the melanin decolorization activity of JS18 strain originated from the laccase. Syringic acid and CuSO4 induced maximum laccase production, yielding 98 U/ml laccase activity after cultivation for 7 days at 25℃. T. velutina secretes an extracellular laccase in GYP medium, and this enzyme was purified using (NH4)2SO4 precipitation, Hi-trap Q Sepharose columns and gel filtration. The molecular weight of the purified enzyme was estimated to be 67 kDa using sodium dodecyl sulfate polyacrylamide gel electrophoresis. This enzyme produced 80% of its melanin decolorization activity within the first 24 h of evaluation in the presence of 1-hydroxybenzotriazole (HBT), while only about 4% of the melanin was decolorized in the absence of the mediator. The greatest decolorization was observed at 1.5 mM/l HBT, which decolorized 81% of the melanin within the first 24 h. The optimum pH and temperature for this decolorization were found to be 5.0 and 37℃, respectively. Our results suggest the possibility of applying HBT induced T. velutina JS18 laccase-catalyzed melanin decolorization.

• Clean Technology
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• v.26 no.4
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• pp.270-278
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• 2020

Palladium (Pd) has been widely used in various industrial applications such as jewelry, catalyst, and dental materials despite its limited resources. It has been gaining attention to recover Pd with high purity from the spent materials. This study investigated the optimum conditions for the leaching and recovery of metallic Pd. The leaching parameters are HCl concentration, temperature, time, concentration of oxidants, and pulp density. 97.2% of Pd leaching efficiency was obtained in 3 M HCl with 3 vol% oxidants at 80℃ for 60 min. The ratio of hydrogen peroxide to sodium hypochlorite played a critical role in the leaching efficiency due to the supply of Cl- ions in the leachate. Moreover, the complete recovery of Pd in the leachate was achieved at 80℃ with 0.3 formic acid/leachate after adjusting the pH value of 7. This situation was ascribed to the decomposition of formic acid into hydrogen gas and carbon dioxide at 80℃. ICP-AES and XRD characterized the recovered Pd powder, and the purity of the recovered powder was found to be 99.6%. Consequently, the recovered Pd powder with high purity could be used in circuits, catalyst precursors, and surgical instruments.

• Journal of The Korean Society of Grassland and Forage Science
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• v.40 no.4
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• pp.251-258
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• 2020

To investigate the suitable growing area for Italian ryegrass (cv. Kowinearly and cv. Green-Call), we survey the different effects of climatic conditions on plant growth in the east (hereafter termed Yeongdong) and west (hereafter termed Yeongseo) of the passes in Gangwon Province. The Italian ryegrass was grown in Gangneung of Yeongdong and Wonju and Chuncheon of Yeongseo. The plants showed growth differences depending on the cultivated regions between before and after wintering. The Italian ryegrass for pre-wintering showed the relatively long length of plant height in Wonju. While, we observed the relatively tall plants for growing- and harvesting-period after wintering in Gangneung. The increased plant height in Gangneung was closely related to all climatic conditions for the growing period and mean- and lowest-temperature for the harvesting period, respectively. The amount of dry matter was 7,490 kg/ha for Kowinearly and 6,490 kg/ha for Green-Call in Gangneung, which has a higher yield than Chuncheon and Wonju. The relative yield index of dry matter was 77% (Kowinearly) and 78% (Green-Call) in Chuncheon and 84% (Kowinearly) and 71% (Green-Call) in Wonju compared to Gangneung as the standard region. Thus, we suggest that Chuncheon and Wonju are the possible areas for cultivation of Italian ryegrass, considering that Gangneung was the optimum growing area in Gangwon Province.

• Journal of Bio-Environment Control
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• v.31 no.1
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• pp.1-7
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• 2022

Strawberry is a stand-out cultivating fruit in Korea. The optimum production of strawberry is highly dependent on growing environment. Smart farm technology, and automatic monitoring and control system maintain a favorable environment for strawberry growth in greenhouses, as well as play an important role to improve production. Moreover, physiological parameters of strawberry plant and it is surrounding environment may allow to give an idea on production of strawberry. Therefore, this study intends to build a machine learning model to predict strawberry's yield, cultivated in greenhouse. The environmental parameter like as temperature, humidity and CO₂ and physiological parameters such as length of leaves, number of flowers and fruits and chlorophyll content of 'Seolhyang' (widely growing strawberry cultivar in Korea) were collected from three strawberry greenhouses located in Sacheon of Gyeongsangnam-do during the period of 2019-2020. A predictive model, Lasso regression was designed and validated through 5-fold cross-validation. The current study found that performance of the Lasso regression model is good to predict the number of flowers and fruits, when the MAPE value are 0.511 and 0.488, respectively during the model validation. Overall, the present study demonstrates that using AI based regression model may be convenient for farms and agricultural companies to predict yield of crops with fewer input attributes.

• Journal of the Korean Wood Science and Technology
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• v.32 no.4
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• pp.58-65
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• 2004

This study was performed to investigate the protease activity from fruit body of Sarcodon aspratus and its features. The specific protease activity was increased with the increasing purification steps, 2.62 times by desalting, 17 times by CMC column chromatography, 113.8 times by DEAE-Sephadex A-50 column chromatography, and 728.3 times by Sephadex G-75 column chromatography. Proteases were identified as two different enzymes having different isoelectric points at pH 4.35 (its recovery rate 8%) and pH 4.7 (its recovery rate 3.5%). Those proteases were purified by 3,025 folds and 3,257 folds in terms of specific activity. Two proteases having different isoelectric points had similar enzymatic properties. This protease was estimated to be 43,000 daltons of molecular weights by SDS-PAGE. This protease with optimum pH 4 was almost stable in the pH range of 4~7. Optimal temperature of protease activity was 40 to 50℃, and the protease activity was completely inhibited at 70℃ for 30 min.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.5
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• pp.608-618
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• 1996

Wild perennial types of genus Glycine, as sources of genetic diversity, have been recently employed in the soybean breeding programs. This study was carried out to introduce some useful characters of wild perennial Glycine tomentella into common cultivars, G. max. Parent materials used for interspecific hybridization were three common cultivars, Ulsankong, Baemkong and Danyeobkong, and four G. tomentella strains having different diploid(2n) of 38, 40, 78, and 80. Their cross-fertility, effect of reciprocal cross, development comparison of crossing pod and optimum timing to efficient crossing were investigated. Plant were grown in 1/2,000 Wagner pots in greenhouse and field. Crossing efficiency and pod survival rate were the highest in 2n=38 of the four G. tomentella strains when the common cultivars were fixed as female parents. In the reciprocal cross of three common cultivars and four G. tomentella strains, when the former was used as maternal sources, they showed better crossing rate and longer pod survival than the latter. In the interspecific hybridization between G. max cv. Baemkong andG. tomentella, 2n=38, the pod formed from the female G. tomentella had relatively greater size and weight than that from the female Baemkong in comparison with parents. However, the reverse result was true in a number of ovules per pod. More efficient interspecific hybridization in the field was made on August 20, when temperature and relative humidity were higher and day length was shorter, than one or two months earlier.