• 한국소음진동공학회논문집
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• 제23권12호
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• pp.1035-1044
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• 2013

면내 하중을 지지하는 면재와 면외 하중을 지지하는 심재로 구성되는 샌드위치 패널 구조물은 높은 비강도와 비강성을 가지므로 경량화가 요구되는 대형 구조물에 자주 이용된다. 그러나, 이러한 구조물은 필연적으로 높은 하중에 대하여 유연성의 증가를 일으키게 되므로, 이에 대한 구조 안전성 분석이 이루어져야 한다. 이에 대해 실제 풍하중은 거스트 영향 등을 비롯한 비선형성을 가지는 요소들이 고려되어야 하며, 구조물의 안전성 분석을 위하여 입력 하중에 대해 보다 실제 물리현상에 근접하게 모사되어야 한다. 이에 이 연구에서는 유체-구조 연성해석 기법을 이용하여 대형 등격자-보강 패널 구조물에 대한 구조 안전성 분석이 수행되었다. 입력하중인 풍하중에 대하여 보다 실제적 모사를 위해 불규칙 변동 속도성분인 거스트 영향이 고려된 랜덤분포 풍하중에 대한 유동장을 생성하여 압력-변위 사상을 통하여 연성해석이 수행되었다.

• Molecules and Cells
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• 제43권3호
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• pp.286-297
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• 2020

Mammalian patatin-like phospholipase domain containing proteins (PNPLAs) play critical roles in triglyceride hydrolysis, phospholipids metabolism, and lipid droplet (LD) homeostasis. PNPLA7 is a lysophosphatidylcholine hydrolase anchored on the endoplasmic reticulum which associates with LDs through its catalytic region (PNPLA7-C) in response to increased cyclic nucleotide levels. However, the interaction of PNPLA7 with LDs through its catalytic region is unknown. Herein, we demonstrate that PNPLA7-C localizes to the mature LDs ex vivo and also colocalizes with pre-existing LDs. Localization of PNPLA7-C with LDs induces LDs clustering via non-enzymatic intermolecular associations, while PNPLA7 alone does not induce LD clustering. Residues 742-1016 contains four putative transmembrane domains which act as a LD targeting motif and are required for the localization of PNPLA7-C to LDs. Furthermore, the N-terminal flanking region of the LD targeting motif, residues 681-741, contributes to the LD targeting, whereas the C-terminal flanking region (1169-1326) has an anti-LD targeting effect. Interestingly, the LD targeting motif does not exhibit lysophosphatidylcholine hydrolase activity even though it associates with LDs phospholipid membranes. These findings characterize the specific functional domains of PNPLA7 mediating subcellular positioning and interactions with LDs, as wells as providing critical insights into the structure of this evolutionarily conserved phospholipid-metabolizing enzyme family.

• Nuclear Engineering and Technology
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• 제51권5호
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• pp.1417-1427
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• 2019

In this study, we evaluated the performance of a commercial pixelated cadmium zinc telluride (CZT) detector for spectroscopy and identified its feasibility as a Compton camera for radiation monitoring in a nuclear power plant. The detection system consisted of a $20mm{\times}20mm{\times}5mm$ CZT crystal with $8{\times}8$ pixelated anodes and a common cathode, in addition to an application specific integrated circuit. The performance of the various radioisotopes $^{57}Co$, $^{133}Ba$, $^{22}Na$, and $^{137}Cs$ was evaluated. In general, the amplitude of the induced signal in a CZT crystal depends on the interaction position and material non-uniformity. To minimize this dependency, a drift time correction was applied. The depth of each interaction was calculated by the drift time and the positional dependency of the signal amplitude was corrected based on the depth information. After the correction, the Compton regions of each spectrum were reduced, and energy resolutions of 122 keV, 356 keV, 511 keV, and 662 keV peaks were improved from 13.59%, 9.56%, 6.08%, and 5%-4.61%, 2.94%, 2.08%, and 2.2%, respectively. For the Compton imaging, simulations and experiments using one $^{137}Cs$ source with various angular positions and two $^{137}Cs$ sources were performed. Individual and multiple sources of $^{133}Ba$, $^{22}Na$, and $^{137}Cs$ were also measured. The images were successfully reconstructed by weighted list-mode maximum likelihood expectation maximization method. The angular resolutions and intrinsic efficiency of the $^{137}Cs$ experiments were approximately $7^{\circ}-9^{\circ}$ and $5{\times}10^{-4}-7{\times}10^{-4}$, respectively. The distortions of the source distribution were proportional to the offset angle.

• BMB Reports
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• 제36권5호
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• pp.505-507
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• 2003

One of the small proteins from Helicobacter pylori, acyl carrier protein (ACP), was investigated by NMR. ACP is related to various cellular processes, especially with the biosynthesis of fatty acid. The basic NMR resonance assignment is a prerequisite for the validation of a heterologuous protein interaction with ACP in H.pylori. Here, the results of the backbone $^1H$, $^{15}N$, and $^{l3}C$ resonance assignments of the H. pylori ACP are reported using double- and triple-resonance techniques. About 97% of all of the $^1HN$, $^{15}N$, $^{13}CO$, $^{13}C{\alpha}$, and $^{13}C{\beta}$ resonances that cover 76 of the 78 non-proline residues are clarified through sequential- and specific-assignments. In addition, four helical regions were clearly identified on the basis of the resonance assignments.

• 한국가시화정보학회지
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• 제3권2호
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• pp.51-56
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• 2005

Acoustic excitations were imposed to coaxial air jet of non-premixed jet flame with hydrogen gaseous injected axially in the center of the flow. The frequencies of excitation were three dominant resonant frequencies at 1L, 2L, 3L. modes including specially 514 Hz (2L-mode) which was estimated theoretically as longitudinal mode of combustor characteristics. The mixing enhancement by acoustic forcing has been investigated quantitatively using PLIF and PIV. The effect of acoustic excitation on combustion process was significant to enhance mixing rate that coincides with specific resonant frequencies. And the behavior of vortex-interaction on flame structure was a good evidence to investigate the phenomenon of shear/mixing layer of fuel-air jet structure. The results obtained in this study concludes that generated streamwise vortex by acoustic excitation has a potential to enhance the mixing rate and abating NOx emissions.

• Journal of Radiation Protection and Research
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• 제45권1호
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• pp.35-44
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• 2020

Background: Cadmium zinc telluride (CZT) is a promising material because of a high detection efficiency, good energy resolution, and operability at room temperature. However, the cost of CZT dramatically increases as its size increases. In this study, to achieve a large effective volume with relatively low cost, an array structure comprised of individual virtual Frisch-grid CZT detectors was proposed. Materials and Methods: The prototype consisted of 2 × 2 CZTs, a holder, anode and cathode printed circuit boards (PCBs), and an application-specific integrated circuit (ASIC). CZTs were used and the non-contacting shielding electrode method was applied for virtual Frisch-grid effect. An ASIC was used, and the holder and the PCBs were fabricated. In the current system, because the CZTs formed a common cathode, a total of 5 channels were assigned for data processing. Results and Discussion: An experiment using ¹³⁷Cs at room temperature was conducted for 10 minutes. Energy and timing information was acquired and the depth of interaction was calculated by the timing difference between the signals of both electrodes. Based on obtained three-dimensional position information, the energy correction was carried out, and as a result the energy spectra showed the improvements. In addition, a Compton image was reconstructed using the iterative method. Conclusion: The virtual Frisch-grid CZT detector based on the array structure was developed and the energy spectra and the Compton image were successfully acquired.

• Steel and Composite Structures
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• 제21권3호
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• pp.605-628
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• 2016

The energy absorption characteristics of diamond core sandwich cylindrical columns under axial crushing process depend greatly on the amount of material which participates in the plastic deformation. Both the single-objective and multi-objective optimizations are performed for columns under axial crushing load with core thickness and helix pitch of the honeycomb core as design variables. Models are optimized by multi-objective particle swarm optimization (MOPSO) algorithm to achieve maximum specific energy absorption (SEA) capacity and minimum peak crushing force (PCF). Results show that optimization improves the energy absorption characteristics with constrained and unconstrained peak crashing load. Also, it is concluded that the aluminum tube has a better energy absorption capability rather than steel tube at a certain peak crushing force. The results justify that the interaction effects between the honeycomb and column walls greatly improve the energy absorption efficiency. A ranking technique for order preference (TOPSIS) is then used to sort the non-dominated solutions by the preference of decision makers. That is, a multi-criteria decision which consists of MOPSO and TOPSIS is presented to find out a compromise solution for decision makers. Furthermore, local and global sensitivity analyses are performed to assess the effect of design variable values on the SEA and PCF functions in design domain. Based on the sensitivity analysis results, it is concluded that for both models, the helix pitch of the honeycomb core has greater effect on the sensitivity of SEA, while, the core thickness has greater effect on the sensitivity of PCF.

• 한국가시화정보학회지
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• 제13권1호
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• pp.35-42
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• 2015

CD4+ T-cell count determines the effectiveness for antiretroviral therapy (ART) in patients with human immunodeficiency virus (HIV). Although ART slows the progression of HIV to AIDS, rapid counting of CD4+ T lymphocytes with a drop of patient's blood sample is urgently needed to ensure timely ART treatment in rural areas. Recently point-of-care CD4 testing devices have been developed by using non-flow based imaging cytometer incorporated with a sample cartridge where CD4+ T cells are reacted with fluorescently tagged specific antibodies. Here we conducted an experimental study using a conventional fluorescence microscope-based imaging system to quantitate the interaction of CD4 antibodies with CD4+ T cells at different reaction conditions. We demonstrated that a fast and affordable point-of-care CD4 test is feasible with a far less amount of antibodies and a shorter incubation time compared with a conventional sample preparation protocol for flow cytometry. We also proposed a general method to evaluate and compare the detection limit across different CD4 counting platforms by using fluorescently labelled microbeads for intensity calibration.

• The Plant Pathology Journal
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• 제31권1호
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• pp.1-11
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• 2015

Antimicrobial cyclic peptides derived from microbes bind stably with target sites, have a tolerance to hydrolysis by proteases, and a favorable degradability under field conditions, which make them an attractive proposition for use as agricultural fungicides. Antimicrobial cyclic peptides are classified according to the types of bonds within the ring structure; homodetic, heterodetic, and complex cyclic peptides, which in turn reflect diverse physicochemical features. Most antimicrobial cyclic peptides affect the integrity of the cell envelope. This is achieved through direct interaction with the cell membrane or disturbance of the cell wall and membrane component biosynthesis such as chitin, glucan, and sphingolipid. These are specific and selective targets providing reliable activity and safety for non-target organisms. Synthetic cyclic peptides produced through combinatorial chemistry offer an alternative approach to develop antimicrobials for agricultural uses. Those synthesized so far have been studied for antibacterial activity, however, the recent advancements in powerful technologies now promise to provide novel antimicrobial cyclic peptides that are yet to be discovered from natural resources.

• Genomics & Informatics
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• 제10권3호
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• pp.153-166
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• 2012

A variety of ligands differ in their capacity to bind the receptor, elicit gene expression, and modulate physiological responses. Such receptors include Toll-like receptors (TLRs), which recognize various patterns of pathogens and lead to primary innate immune activation against invaders, and G-protein coupled receptors (GPCRs), whose interaction with their cognate ligands activates heterotrimeric G proteins and regulates specific downstream effectors, including immuno-stimulating molecules. Once TLRs are activated, they lead to the expression of hundreds of genes together and bridge the arm of innate and adaptive immune responses. We characterized the gene expression profile of Toll-like receptor 4 (TLR4) in RAW 264.7 cells when it bound with its ligand, 2-keto-3-deoxyoctonate (KDO), the active part of lipopolysaccharide. In addition, to determine the network communications among the TLR, Janus kinase (JAK)/signal transducer and activator of transcription (STAT), and GPCR, we tested RAW 264.7 cells with KDO, interferon-${\beta}$, or cAMP analog 8-Br. The ligands were also administered as a pair of double and triple combinations.