• Journal of Microbiology and Biotechnology
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• 제23권12호
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• pp.1791-1801
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• 2013

Diketopiperazine is produced by various organisms, including bacteria, fungi, and animals, and has been suggested as a novel signal molecule involved in the modulation of genes with various biological functions. Vibrio vulnificus, which causes septicemia in humans, produces cyclo($\small{L}$-phenylalanine-$\small{L}$-proline) (cFP). To understand the biological roles of cFP, the effect of the compound on the expression of the total mRNA in V. vulnificus was assessed by next-generation sequencing. Based on the transcriptomic analysis, we classified the cFP-regulated genes into functional categories and clustered them according to the expression patterns resulted from treatment with cFP. From a total of 4,673 genes, excepting the genes encoding tRNA in V. vulnificus, 356 genes were up-regulated and 602 genes were down-regulated with an RPKM (reads per kilobase per million) value above 3. The genes most highly induced by cFP comprised those associated with the transport and metabolism of inorganic molecules, particularly iron. The genes negatively regulated by cFP included those associated with energy production and conversion, as well as carbohydrate metabolism. Noticeably, numerous genes related with biofilm formation were modulated by cFP. We demonstrated that cFP interferes significantly with the biofilm formation of V. vulnificus.

• Journal of Plant Biotechnology
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• 제43권3호
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• pp.311-316
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• 2016

The efficacy of plant breeding has been enhanced by application of molecular markers in population screening and selection. Pepper (Capsicum annuum L.) is a major staple crop that is economically important with worldwide distribution. It is valued for its spicy taste and medicinal effect. The aim of this study was to discover single nucleotide polymorphisms (SNPs), microsatellite markers information, and percentage sharing through orthologous analysis of pepper-specific pungency-related genes. Here, we report the results of transcriptome analysis and microsatellite markers for four pepper varieties that possess a pungency-related gene. Orthologous analyses was performed to identify species-specific pungency-related genes in pepper, Arabidopsis thaliana L., potato (Solanum tuberosum L.), and tomato (Solanum lycopersicum L.). Advancements in next-generation sequencing technologies enabled us to quickly and cost-effectively assemble and characterize genes to select molecular markers in various organisms, including pepper. We identified a total of 9762, 7302, 8596, and 6886 SNPs for the four pepper cultivars Blackcluster, Mandarine, Saengryeg 211, and Saengryeg 213, respectively. We used 454 GS-FLX pyrosequencing to identify microsatellite markers and tri-nucleotide repeats (54.4%), the most common repeats, followed by di-, hexa-, tetra-, and penta-nucleotide repeats. A total of 5156 (15.9%) pepper-specific pungency-related genes were discovered as a result of orthologous analysis.

• Journal of applied mathematics & informatics
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• 제32권3_4호
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• pp.389-403
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• 2014

In this paper, we first provide comparison results of preconditioned AOR methods with direct preconditioners $I+{\beta}L$, $I+{\beta}U$ and $I+{\beta}(L+U)$ for solving a linear system whose coefficient matrix is a large sparse irreducible L-matrix, where ${\beta}$ > 0. Next we propose how to find a near optimal parameter ${\beta}$ for which Krylov subspace method with these direct preconditioners performs nearly best. Lastly numerical experiments are provided to compare the performance of preconditioned iterative methods and to illustrate the theoretical results.

• 설비공학논문집
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• 제15권5호
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• pp.389-396
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• 2003

Systematic methods to calculate thermodynamic properties of carbon dioxide, HFC-l34a and HCFC-22 are presented. First, application of a basic method to identify the saturation state with given temperature or pressure is attempted and the feasibility of auxiliary equations is tested. Next, detailed procedures are suggested to tell a phase when temperature/pressure and another property are specified. Finally Newton-Raphson method is applied to calculate unknown thermodynamic properties fixing the state with the two independent properties specified. The procedures described here are utilized to develop a computer program, which is used to find the relation between temperature and pressure with maximum isobaric heat capacity for super-critical carbon dioxide.

• 대한임베디드공학회논문지
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• 제13권3호
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• pp.125-131
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• 2018

STT-RAM is attracting as a next generation Non-volatile memory for replacing cache memory with low leakage energy, high integration and memory access performance similar to SRAM. However, there is problem of write operations as the other Non_volatile memory. Hybrid cache memory using SRAM and STT-RAM is attracting attention as a cache memory structure with lowe power consumption. Despite this, reducing the leakage energy consumption by the STT-RAM is still lacking access to the Dynamic energy. In this paper, we proposed as energy management method such as a way-selection approach for hybrid L2 cache fo SRAM and STT-RAM and memory selection method of write/read operation. According to the simulation results, the proposed hybrid cache memory reduced the average energy consumption by 40% on SPEC CPU 2006, compared with SRAM cache memory.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2008년 영문 학술대회
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• pp.235-242
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• 2008

An innovative Panel ExTension SATellite(PETSAT) and propulsion system for PETSAT, are presented in this paper. First, we outline what PETSAT is. Next, based on PETSAT ethos, design policy of the propulsion system is provided. According to the policy, we designed propulsion system and concretely estimated and assembled mono-propellant and bi-propellant systems, and it indicated that mono-propellant propulsion with 50-60 seconds of specific impulse and 1 N of thrust is probable. In the case of bi-propellant, 120-150 seconds of specific impulse is valid even based on the design policy. We conducted captive tests of mono-propellant and bi-propellant propulsions with a breadboard model of propulsion system for PETSAT, and obtained good operations and performances. Based on the test results, we designed and manufactured flight model propulsion system for PETSAT. We are planning to demonstrate it in SOHLA-2L project progressed by the Space Oriented Higashiosaka Leading Association(SOHLA). SOHLA-2L will be the first on-orbit demonstrator of PETSAT in 2008.

• 대한미생물학회지
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• 제5권1호
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• pp.1-8
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• 1970

This report is to observe the rate of multiple resistance to various antibiotics among staphylococcus aureus and E. coli which were isolated with high frequency at our department during 1969. 1. One hundred fifty two strains(20.6%) resistant to four antibiotics multiple resistant was the highest incidence among 739 strains of Staphylococcus aureus. 2. Regarding the multiple antibiotic resistant of Staphylococcus aureus, 75strains(10.1%) to SA. PC. TC the three antibiotics resistant, have been observed, with the highest incidence, and next to this, 69 strains(9.3%) to SA. PC. SM. TC the four autibiotics resistant, and 68 strains(9.2%) to SA. PC. SM. CM. TC. SM. the six antibiotics resistant, have been observed in descending order. 3. The case of one hundred twenty seven strains(28.0%) of the six antibiotic multiple resistant among 454 strains of Escherchia coli was the highest incidence. 4. Concerning the multiple antibiotic resistant of Escherichia coli, 70 strains(l5.4%) to SA. SM. CM. TC. EM. FD. the six antibiotics resistant, have been observed, with the highest incidence, and next to this, 59 strains(l3.0%) to SA. SM. CM. TC. EM. FD. AC the seven antibiotics resistant, and 42 strains(9.3%) to SA. SM. CM. TC. EM. FD. KM. AC the eight antibiotics resistant have been observed in descending order.

• International Journal of Industrial Entomology and Biomaterials
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• 제11권2호
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• pp.139-143
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• 2005

The implications of temperature (25, 30 and $35^{\circ}C$) and relative humidity (RH; 60, 70 and $80\%$) on the hatching rhythmicity and hatching parameters (percentage and duration) were studied in the silkworm, Bombyx mori L. under natural photoperiod (LD 12 : 12). Disease free layings (DFLs) of two pure silkworm breeds, Pure Mysore (PM, a multivoltine breed) and $NB_4D_2$ (a bivoltine breed), and their hybrid, $PM{\times}NB_4D_2$ were introduced into the experimental conditions on the $3^{rd}$ day of oviposition till completion of hatching. The hatching rhythm was predominantly diurnal under all temperature and humidity conditions, with peaks just after 'lights-on' phase (6 hrs). Extreme temperature and humidity conditions did not alter the hatching rhythmicity, but prolonged the hatching durations, extending it to the next day, coupled with reduced hatching percentage in PM and $PM{\times}NB_4D_2{\cdot}In\;NB_4D_2$, on the other hand, hatching did not extend to the next day. Hatching percentage in this breed, however, reduced below the economic level under high temperature and low humidity conditions. The high temperature and low humidity together, though did not alter the rhythmicity, seems to exert synergetic effect on the hatching percentage and its duration in the silkworm, B. mori.

• 대한산업공학회지
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• 제33권4호
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• pp.457-468
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• 2007

This paper proposes an adaptive moving average (A-MA) control chart with variable sampling intervals (VSI) for detecting shifts in the process mean. The basic idea of the VSI A-MA chart is to adjust sampling intervals as well as to accumulate previous samples selectively in order to increase the sensitivity. The VSI A-MA chart employs a threshold limit to determine whether or not to increase sampling rate as well as to accumulate previous samples. If a standardized control statistic falls outside the threshold limit, the next sample is taken with higher sampling rate and is accumulated to calculate the next control statistic. If the control statistic falls within the threshold limit, the next sample is taken with lower sampling rate and only the sample is used to get the control statistic. The VSI A-MA chart produces an 'out-of-control' signal either when any control statistic falls outside the control limit or when L-consecutive control statistics fall outside the threshold limit. The control length L is introduced to prevent small mean shifts from being undetected for a long period. A Markov chain model is employed to investigate the VSI A-MA sampling process. Formulae related to the steady state average time-to signal (ATS) for an in-control state and out-of-control state are derived in closed forms. A statistical design procedure for the VSI A-MA chart is proposed. Comparative studies show that the proposed VSI A-MA chart is uniformly superior to the adaptive Cumulative sum (CUSUM) chart and to the Exponentially Weighted Moving Average (EWMA) chart, and is comparable to the variable sampling size (VSS) VSI EWMA chart with respect to the ATS performance.

• 한국경영과학회:학술대회논문집
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• 한국경영과학회 2006년도 추계학술대회
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• pp.560-570
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• 2006

This paper proposes a selectively cumulative sum (S-CUSUM) control chart with variable sampling intervals (VSI) for detecting shifts in the process mean. The basic idea of the VSI S-CUSUM chart is to adjust sampling intervals and to accumulate previous samples selectively in order to increase the sensitivity. The VSI S-CUSUM chart employs a threshold limit to determine whether to increase sampling rate as well as to accumulate previous samples or not. If a standardized control statistic falls outside the threshold limit, the next sample is taken with higher sampling rate and is accumulated to calculate the next control statistic. If the control statistic falls within the threshold limit, the next sample is taken with lower sampling rate and only the sample is used to get the control statistic. The VSI S-CUSUM chart produces an 'out-of-control' signal either when any control statistic falls outside the control limit or when L-consecutive control statistics fall outside the threshold limit. The number L is a decision variable and is called a 'control length'. A Markov chain model is employed to describe the VSI S-CUSUM sampling process. Some useful formulae related to the steady state average time-to signal (ATS) for an in-control state and out-of-control state are derived in closed forms. A statistical design procedure for the VSI S-CUSUM chart is proposed. Comparative studies show that the proposed VSI S-CUSUM chart is uniformly superior to the VSI CUSUM chart or to the Exponentially Weighted Moving Average (EWMA) chart with respect to the ATS performance.