• BMB Reports
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• 제30권3호
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• pp.188-193
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• 1997

Membrane lipid peroxidation processes yield products that may react with DNA to cause mutations. Lipid hydroperoxides from linoleic acid in the presence of transition metal ions caused strand breaks in plasmid DNA. DNA damage induced by reactive aldehydes known to be produced by decomposition of lipid hydroperoxides, such as 4-hydroxynonenal or rnalondialdehyde, was repaired by endonucleases and exonuclease III which resulted in the increase of single strand breaks in DNA. Lipid hydroperoxides as well as malondialdehyde and 4-hydroxynonenal also caused mutations in the pUC18 lacZ' gene when measured as a loss of ${\alpha}-cornplementation$. In conclusion. the lipid peroxidation could be an important intermediary event in DNA damage and mutation by oxidative stress.

• 상하수도학회지
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• 제10권1호
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• pp.96-111
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• 1996

The failure of water pipelines is progressed by several compound factors and the collection and analysis of data about pipeline failure are inevitable for effective pipeline rehabilitation. Data analysis of pipeline failure was already performed in USA and Europe. Based on such phenomena, failure characteristics about metropolitan pipelines in Korea were analyzed: The conclusions of this study are as followings. 1. The failure cause of pipelines can be classified into natural cause and artificial cause. Artificial cause is 32% of total causes, so artificial failure as several constructions happens frequently in Korea. Although the failure by old pipe is greatest of any other causes m classtfied cause, failure cause is not classified in detail now. 2. The damaged part of pipelines is affected by cities, distribution system inventory, bedding conditions, and so on. In this study, the failure of pipeline body(67%) is greater than the failure of pipeline joint(33%) in natural failure. 3. In regard to pipe materials, failure rate of DCIP(0.8456), PEP(0.7288), and GSP(0.6643) is greater than that of CIP(0.3985) and CWSP(0.2348). 4. Usually, faIlure rate is increased in proportion to diameter of pipeline. In this study, CIP, DCIP, and CWSP have clear trends. But the trends of PEP is reverse, the case of GSP, HP is obscure due to data shortage. 5. There are no direct relationships between burial age and failure rate of pipelines. 6. Annual breaks and winter(Nov.~Feb.) breaks of pipelines are investigated. As a result, WInter breaks to annual breaks of CIP is 51.3%(Seoul), 51.1%(Taegu),38.7%(Pusan). This phenomena have direct correlation with average winter temp. of cities.

• 한국화재소방학회논문지
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• 제33권6호
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• pp.63-71
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• 2019

본 연구에서는 화재발생 현황 및 용도별 건축물 통계데이터를 활용하여 전국의 용도별 건축물의 화재위험도를 정량적으로 분석하였다. 화재 및 건축물 통계는 최근 10년간(2008~2017) 데이터를 이용하였으며, 「건축법」과 「소방시설 설치유지 및 안전관리에 관한 법률 시행령」을 통해 용도를 분류하여 자연적 구분법(Natural breaks)으로 상대평가를 실시하여 리스크 매트릭스로 나타내었다. 또한, 본 연구에서 도출한 리스크 매트릭스의 적합성을 검증하기 위하여 2018년도 위험도 등급을 지난 10년 데이터와 비교·분석을 진행한 결과, 위락시설에서 위험도가 IV등급에서 II 등급으로 감소한 결과를 제외하고는 대부분의 시설이 유사한 위험도를 나타내고 있어 과거와 현재의 데이터 상관성이 유효한 것을 확인하여 분류방법이 적절한 것으로 판단하였다. 본 연구에서 제안된 자연적 구분법을 이용한 화재위험도 평가방법은 화재예방에 활용할 수 있을 것으로 확인되었다.

• Biomolecules & Therapeutics
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• 제20권5호
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• pp.487-491
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• 2012

The aim of this study was to investigate the inhibitory effects of rutaecarpine on DNA strand breaks and apoptosis induced by hydrogen peroxide ($H_2O_2$) in murine Hepa-1c1c7 cells. Oxidative DNA damage was estimated by nuclear condensation assessment, fluorescence-activated cell sorting analysis, and Comet assay. Rutaecarpine inhibited cell death induced by $500{\mu}M$ $H_2O_2$, as assessed by 4',6-diamidino-2-phenylindole (DAPI) staining. Treatment with rutaecarpine reduced the number of DNA strand breaks induced by $H_2O_2$, as assessed by DAPI staining and Comet assay, and increased quinone reductase, phosphatidylinositol 3-kinase, and pAkt protein levels, as assessed by western blotting.

• 인지과학
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• 제19권1호
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• pp.89-105
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• 2008

본 논문에서는 자연스러운 한국어 운율구 경계를 예측하기 위해 (1) 문장 성분을 하위범주화하고, (2) 세분화된 문장 성분 간 의존관계를 이용하여 통사구를 추출하며 (3) 추출한 통사구의 유형에 따른 운율구 경계 예측 규칙을 설정하였다. 또한, (4) 통사적 정보 외에도 통사구와 문장의 길이, 통사구의 문장 내 위치, 문맥의 의미 정보 등에 따라 가변적인 운율구 경계를 판단하여 보다 자연스러운 한국어 운율구 경계 예측 시스템을 개발하였다. 그 결과 통사구 경계와 상관관계가 높은 강한 운율구 경계 예측과 운율구 내부 비경계 예측에 있어 90% 이상의 높은 재현율과 정확도를 보였으며, 전체 운율구 경계 예측에 있어서도 87% 이상의 성능을 보였다.

• 한국잠사곤충학회지
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• 제17권1호
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• pp.1-26
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• 1975

These studies were attempted to find out the optimum silk reeling system by use of automatic silk reeling machine to increase raw silk yield and reeling efficiency with various silk reelable cocoons. The obtained results are as follows; 1. The mean silk reelability ratio(X)of the Korean cocoons during the last ten years was 61 per cent, beside 64.7 per cent in autumn cocoon and 57.3 per cent in spring cocoon. However, the ratio variation of autumn cocoons was larger than that of spring cocoons. 2. A positive correlation between cocoon filament breaks during its process and silk reelability levels was shown to be significant. The cocoons of both poor and good reelability evidenced "J" shape distribution on the filament break graph by the order of reeling cocoon end. Many bave breaks were found at the inner shell of the cocoons, or in case of poor reelability cocoons. 3. The morphology of broken cocoon ends during the process was classified into A, B, C, D, E and F types, The occurrence of B type was majority, but that of F type was minority among them. 4. In case of the cocoon cooking, H-type ion-exchanged soft water was better for good reelability cocoons, Na and H-type ion exchanged neutral soft water for those of fair reelability, and alkaline (Na-type) soft water for those of poor reelability, respectively. 5. The modification of cooking water by mixing the above different types of water (50％ Na-type and 50％ H-type passed by standard natural water; 75％ Na-type and 25％ H-type passed by hard natural water; 25％ Na-type and 75％ H-type passed by soluble natural water) made higher yield of raw silk with tess breaks of thread. 6, In case cocoon ends groping water included sodium hexametaphosphate as much as 800 ppm. the groping efficiency and raw silk yield of cocoon was improved. The effect was pronounced in case of poor reelability cordons. 7. The most reasonable cocoon cooking and silk reeling condition for automatic silk reeling process were observed to be rather incomplete cook with good reelability cocoons and optimum cook with poor reelability cocoons succeeded by the reeling bath temperature of 45$^{\circ}C$, 8. The reasonable silk reeling velocities were observed to be about 150m per min. for good reelability cocoons, 120m per min. for fair reelability ones and 90 to 120m per min. for poor reelability ones. 9. In order to improve the raw silk yield of cocoons and reeling efficiency, the cocoon stand-by-ratio for reeling should be kept at the level of 40 per cent for good reelability cocoons or at 60 per cent for poor reelability ones beside necessary end found cocoon condition.

• BMB Reports
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• 제55권11호
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• pp.541-546
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• 2022

The repair of DNA double-strand breaks (DSBs) by homologous recombination (HR) is crucial for maintaining genomic integrity and is involved in numerous fundamental biological processes. Post-translational modifications by proteins play an important role in regulating DNA repair. Here, we report that the methyltransferase SET7 regulates HR-mediated DSB repair by methylating TIP60, a histone acetyltransferase and tumor suppressor involved in gene expression and protein stability. We show that SET7 targets TIP60 for methylation at K137, which facilitates DSB repair by promoting HR and determines cell viability against DNA damage. Interestingly, TIP60 demethylation is catalyzed by LSD1, which affects HR efficiency. Taken together, our findings reveal the importance of TIP60 methylation status by SET7 and LSD1 in the DSB repair pathway.

• Journal of Microbiology and Biotechnology
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• 제32권5호
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• pp.663-671
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• 2022

The saccharification of cellulose and hemicellulose is essential for utilizing lignocellulosic biomass as a biofuel. While cellulose is composed of glucose only, hemicelluloses are composed of diverse sugars such as xylose, arabinose, glucose, and galactose. Sulfolobus acidocaldarius is a good potential candidate for biofuel production using hemicellulose as this archaeon simultaneously utilizes various sugars. However, S. acidocaldarius has to be manipulated because the enzyme that breaks down hemicellulose is not present in this species. Here, we engineered S. acidocaldarius to utilize xylan as a carbon source by introducing xylanase and β-xylosidase. Heterologous expression of β-xylosidase enhanced the organism's degradability and utilization of xylooligosaccharides (XOS), but the mutant still failed to grow when xylan was provided as a carbon source. S. acidocaldarius exhibited the ability to degrade xylan into XOS when xylanase was introduced, but no further degradation proceeded after this sole reaction. Following cell growth and enzyme reaction, S. acidocaldarius successfully utilized xylan in the synergy between xylanase and β-xylosidase.

• 한국정밀공학회지
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• 제31권8호
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• pp.697-703
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• 2014

This paper is study of solving vibration problem occurred in moving hand of wafer transfer robot in semiconductor manufacturing line. Long settling time for decreasing vibration makes low production rate, and moreover the excessive vibration of hand sometimes breaks the wafer in a cassette. The ways of reducing the moving speed and changing the type of motion profile did not help for lessening vibration. Therefore, we analyzed the mechanical property of the hand such as natural frequency, and frequency component of the motion profile currently used in the manufacturing line. In several conditions of motion profile, we found the best condition of which the frequency component in near of natural frequency of the hand is minimal and this induced small vibration in moving hand. The results were verified theoretically and experimentally using frequency analysis.

• BMB Reports
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• 제28권3호
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• pp.249-253
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• 1995

The nonenzymatic glycation of copper, zinc-superoxide dismutase (Cu,Zn-SOD) led to inactivation and fragmentation of the enzyme. The glycated Cu,zn-SOD was isolated by boronate affinity chromatography. The formation of 8-hydroxy-2'-deoxyguanosine (8-OH-dG) in calf thymus DNA and the generation of strand breaks in pBhiescript plasmid DNA by a metal-catalyzed oxidation (MCO) system composed of $Fe^{3+}$, $O_2$, and glutathione (GSH) as an electron donor was enhanced more effectively by the glycated CU,Zn-SOD than by the nonglycated enzyme. The capacity of glycated Cu,Zn-SOD to enhance damage to DNA was inhibited by diethylenetriaminepentaacetic acid (DETAPAC), azide, mannitol, and catalase. These results indicated that incubation of glycated CU,Zn-SOD with GSH-MCO may result in a release of $Cu^{2+}$ from the enzyme. The released $Cu^{2+}$ then likely participated in a Fenton-type reaction to produce hydroxyl radicals, which may cause the enhancement of DNA damage.