Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Effects of Rutaecarpine on Hydrogen Peroxide-Induced Apoptosis in Murine Hepa-1c1c7 Cells

  • Lee, Sung-Jin (Department of Animal Biotechnology, Kangwon National University) ;
  • Ahn, Hyun-Jin (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Nam, Kung-Woo (SCH-BioPharm Human Resources Development Center, Soonchunhyang University) ;
  • Kim, Kyeong-Ho (College of Pahrmacy, Kangwon National University) ;
  • Mar, Woong-Chon (Natural Products Research Institute, College of Pharmacy, Seoul National University)
  • Received : 2011.10.28
  • Accepted : 2012.07.30
  • Published : 2012.09.30
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Abstract

The aim of this study was to investigate the inhibitory effects of rutaecarpine on DNA strand breaks and apoptosis induced by hydrogen peroxide ($H_2O_2$) in murine Hepa-1c1c7 cells. Oxidative DNA damage was estimated by nuclear condensation assessment, fluorescence-activated cell sorting analysis, and Comet assay. Rutaecarpine inhibited cell death induced by $500{\mu}M$ $H_2O_2$, as assessed by 4',6-diamidino-2-phenylindole (DAPI) staining. Treatment with rutaecarpine reduced the number of DNA strand breaks induced by $H_2O_2$, as assessed by DAPI staining and Comet assay, and increased quinone reductase, phosphatidylinositol 3-kinase, and pAkt protein levels, as assessed by western blotting.

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References

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