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http://dx.doi.org/10.4062/biomolther.2012.20.5.487

Effects of Rutaecarpine on Hydrogen Peroxide-Induced Apoptosis in Murine Hepa-1c1c7 Cells  

Lee, Sung-Jin (Department of Animal Biotechnology, Kangwon National University)
Ahn, Hyun-Jin (Natural Products Research Institute, College of Pharmacy, Seoul National University)
Nam, Kung-Woo (SCH-BioPharm Human Resources Development Center, Soonchunhyang University)
Kim, Kyeong-Ho (College of Pahrmacy, Kangwon National University)
Mar, Woong-Chon (Natural Products Research Institute, College of Pharmacy, Seoul National University)
Publication Information
Biomolecules & Therapeutics / v.20, no.5, 2012 , pp. 487-491 More about this Journal
Abstract
The aim of this study was to investigate the inhibitory effects of rutaecarpine on DNA strand breaks and apoptosis induced by hydrogen peroxide ($H_2O_2$) in murine Hepa-1c1c7 cells. Oxidative DNA damage was estimated by nuclear condensation assessment, fluorescence-activated cell sorting analysis, and Comet assay. Rutaecarpine inhibited cell death induced by $500{\mu}M$ $H_2O_2$, as assessed by 4',6-diamidino-2-phenylindole (DAPI) staining. Treatment with rutaecarpine reduced the number of DNA strand breaks induced by $H_2O_2$, as assessed by DAPI staining and Comet assay, and increased quinone reductase, phosphatidylinositol 3-kinase, and pAkt protein levels, as assessed by western blotting.
Keywords
Rutaecarpine; Oxidative stress; DNA damage; Quinone reductase; Apoptosis; Hydrogen peroxide;
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