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Breeding of new variety Pleurotus pulmonarius using protoplast fusion technique (원형질체융합 기법을 이용한 산느타리 계통육성)

  • Gwon, Hee-Min;Lee, Yun-Hae;Kim, Jeong-Han;Baek, Il-Sun;Kang, Hee-Wan;Choi, Jong-In
    • Journal of Mushroom
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    • v.19 no.3
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    • pp.166-175
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    • 2021
  • In this study, monokaryons of "Heukari" (Pleurotus ostreatus) and "Hosan" (Pleurotus pulmonarius) were separated to remove the cell wall, and a cross-species protoplast fusion was developed through chemical treatment with polyethylene glycol. The protoplast-fused PF160306 and PF160313 strains have a culture period of 10 and 2 days shorter than that of the "Heuktari" and "Hosan" cultivars, respectively. Furthermore, the growth of the strains was faster than that of the existing cultivars. The yield was 135.9 g per bottle, which was approximately 8% higher than that of the commercially available "Hosan" cultivar; however, it was not statistically significant. A growth survey was conducted after treatment at five temperatures (15, 18, 21, 23, and 25℃). The growth of the strains accelerated with the increase in temperature. However, at 21℃, the yellow color of pileus was the brightest. Band pattern, assessed using URP Primer 7, was similar to that of the "Hosan" cultivar. The DPPH radical scavenging capacity and polyphenol content were 62.5% and 43.5 mg/mL, respectively, for "Sunjung" and 65.7% and 49.9 mg/mL, respectively, for PF160313. Furthermore, the antihypertensive activities of the "Sunjung" cultivar and PF160313 were similarly high at 74% and 75%, respectively. In conclusion, cross-species hybridization via the protoplast fusion technique can be used for obtaining primary data for mushroom breeding to develop new varieties. In addition, the protoplast fusion technique might aid in expanding the market for yellow mushrooms.

Physicohemical Properties of Extruded Rice Flours and a Wheat Flour Substitute for Cookie Application (압출쌀가루의 이화학적 특성 및 밀가루 대체 쿠키 특성)

  • We, Gyoung Jin;Lee, Inae;Kang, Tae-Young;Min, Joo-Hong;Kang, Wie-Soo;Ko, Sanghoon
    • Food Engineering Progress
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    • v.15 no.4
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    • pp.404-412
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    • 2011
  • The purpose of this study is to prepare extruded rice flours suitable for baking rice cookies. The extruded rice flours were prepared at 100 and 130$^{\circ}C$ temperature and 25 and 27% moisture content in a co-rotating twin screw extruder. The rice extrudates were dried at 100$^{\circ}C$ for 18 hr and subsequently ground into the fine flour. Characteristics of the extruded rice flours were examined by rapid visco analysis, hydration property analysis, differential scanning calorimetry (DSC), and in vitro digestion test. Water absorption, solubility, and swelling power of all extruded rice flours were higher than those of native rice flour. DSC analysis showed that native rice flour had a peak at about 65$^{\circ}C$ while all extruded rice flours did not show any peaks since they were already gelatinized during the extrusion proess. Viscosity of the extruded rice flours decreased with increasing temperature and lowering moisture content in the extrusion proess. The extruded rice flours prepared at 130$^{\circ}C$ exhibited lower viscosity than those prepared at 100$^{\circ}C$. The operating temperature of the extrusion proess was critical for the starch digestion in vitro. The extruded rice flours prepared at 130$^{\circ}C$ showed a rapid decrease in digestible starch content while an increased level of slowly digestible starch content was observed compared to those treated at 100$^{\circ}C$ in the extruder. Cookies were prepared with a mixture of wheat flour and extruded rice flours at the ratio of 7 to 3. The cookies made with the extruded rice flours had lower spread factor and darker yellow color than those prepared with wheat flour only. Hardness of the extruded rice flour-added cookies was similar to that of the wheat flour cookie whereas their overall acceptance was better. Therefore the rice cookies partially supplemented with extruded rice flours may have a potential as early childhood foods which require soft texture and allergy reduction.

Notes on the Status and Conservation of Callipogon Relictus Semenov in Korea (장수하늘소 현황 및 보전방안)

  • An, Seung Lak
    • Korean Journal of Heritage: History & Science
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    • v.43 no.1
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    • pp.260-279
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    • 2010
  • The analysis on bibliography and field investigation of Callipogon relictus Semenov, 1898 (Korean natural monument number 218) shows that the size varies from country to country, and Korean specimens, for which male is 85~120mm and female is 65~85mm, are found to be the largest. The average diameter and length of egg are 2.60mm and 6.72mm respectively. The larva has milky color and is about 100~150mm in length. The pupa is nearly 70~110mm. An adult generally appears from June to September in Korea in the broadleaf forest of lowland, whereas it appears from June to July in China. It is known that the pupa largely feed on the old tree trunk of Carpinus laxiflora (Siebold & Zucc.) blume in Korea, but no such data have been reported in China and Russia, showing differences in host plants. While the larva period is not exactly known in Korea, it is reported to be two years in China. It appears that the species inhabits in very limited regions of approximately between geographical latitude $37.5^{\circ}{\sim}47.8^{\circ}$ and longitude $126^{\circ}{\sim}140^{\circ}$ including Korea, China and Russia. To conserve the long-horned beetle in Korea, this research drew out following some conclusions through analyzing the references and field survey data. First, it need to perform precise survey on the natural environment of occurring and collected area or place including host plant kinds, temperate, humidity, latitude, longitude etc. Second, habitat region must be designated as a restricted development area, and it need to exclude or reduce the damage factors to prosper reproduction of the species. Third, it is necessary to keep loosing cautiously artificial breeding individuals in the reported sites, not disturbing scope of natural populations. Fourth, it needs to educate or publicize many people importance and value of this species through many methods.

Shelf Life Extension of Wasabi Paste Products by Addition of Citric Acid (구연산 첨가에 의한 와사비 페이스트 제품의 저장성 향상)

  • Jeong, Eun-Jeong;Lee, Hyo-Kyung;Kim, Yong-Suk
    • Journal of Food Hygiene and Safety
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    • v.34 no.4
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    • pp.354-360
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    • 2019
  • In order to extend the shelf life of Wasabi paste, the effects of citric acid were confirmed at $35^{\circ}C$ for 28 days. Citric acid-treated groups contained citric acid in amounts of 0.05, 0.10, 0.17, 0.30, and 0.40%, respectively. Quality characteristics of Wasabi pastes were determined in pH, titratable acidity, soluble solid content, color values, microbial analysis (aerobic bacteria, yeast), gas production, and content of allyl isothiocyanate. The pH and titratable acidities of Wasabi pastes added with citric acid were indicated as 4.03-5.19 and 4.23-4.82%, respectively. Soluble solid content was significantly different according to concentrations of citric acid. L values showed the highest at $50.05{\pm}0.46$. a and b values were increased during the storage period. Total aerobic bacteria and yeast counts of Wasabi pastes were decreased in a dose-dependent manner. Gas production from Wasabi pastes showed at 19.55-19.80 mL/tube after 28 days of storage. The addition of citric acid (0.3% or more) to the Wasabi paste resulted in increased storage stability.

Effects of Dietary Apparent Metabolizable Energy and Protein Concentrations on Growth Performance, Nutrient Digestibility, and Quality of Korean Native Ducks (사료내 에너지 및 단백질 함량이 토종오리 생산성, 영양소 이용률 및 도체 특성에 미치는 영향)

  • Kim, Chan Ho;Kang, Hwan Ku
    • Korean Journal of Poultry Science
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    • v.46 no.3
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    • pp.205-213
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    • 2019
  • The objective of this experiment was to investigate the effect of dietary apparent metabolizable energy and protein concentrations on growth performance, carcass characteristics, and nutrient digestibility of Korean native ducks. In $2{\times}2$ factorial arrangement, 1-d-old Korean native ducks were completely randomized to experimental diets with 2,800 and 2,900 kcal of AMEn/kgofdiet, respectively, from 0 to 21 d of age. From 22 to 56 d of age, experimental diets 3,000 and 3,100 kcal of, and contained 16% and 17% CP, respectively. Feed and water were provided ad libitum. And BW gain were higher during 0 to 21 d (P<0.01) in AME 2,800 kcal/kg and CP 21% diet treatment than other treatments. As AME increased from 2,800 to 2,900 kcal/kg, feed intake was decreased (P<0.05) during 0 to 21 d. From 22 to 56 d of age, the, BW gain, feed intake and were not different among the treatments. Carcass ratio, relative weight per 100 of BW (wing, back, neck, breast, and leg), nutrient digestibility, meat color and nutrient composition in breast meat the treatments. In conclusion, diets with 2,800 kcal of AME/kg and 21% CP or with 3,000 kcal of AME/kg and 17% CP at 0 to 21 d and 22 to 56 d, respectively, were more efficient.

Techniques and Traditional Knowledge of the Korean Onggi Potter (옹기장인의 옹기제작기술과 전통지식)

  • Kim, Jae-Ho
    • Korean Journal of Heritage: History & Science
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    • v.48 no.2
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    • pp.142-157
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    • 2015
  • This study examines how traditional knowledge functions in the specific techniques to make pottery in terms of the traditional knowledge on the pottery techniques of Onggi potters. It focuses on how traditional pottery manufacturing skills are categorized and what aspects are observed with regard to the techniques. The pottery manufacturing process is divided into the preparation step of raw material, the molding step of pottery, and the final plasticity step. Each step involves unique traditional knowledge. The preparation step mainly comprises the knowledge on different kinds of mud. The knowledge is about the colors and properties of mud, the information on the regional distribution of quality mud, and the techniques to optimize mud for pottery manufacturing. The molding step mainly involves the structure and shape of spinning wheels, the techniques to accumulate mud, ways to use different kinds of tools, the techniques to dry processed pottery. The plasticity step involves the knowledge on kilns and the scheme to build kilns, the skills to stack pottery inside of the kilns, the knowledge on firewood and efficient ways of wood burning, the discrimination of different kinds of fire and the techniques to stoke the kilns. These different kinds of knowledge may be roughly divided into three categories : the preparation of raw material, molding, and plasticity. They are closely connected with one another, which is because it becomes difficult to manufacture quality pottery even with only one incorrect factor. The contents of knowledge involved in the manufacturing process of pottery focused are mainly about raw material, color, shape, distribution aspect, fusion point, durability, physical property, etc, which are all about science. They are rather obtained through the experimental learning process of apprenticeship, not through the official education. It is not easy to categorize the knowledge involved. Most of the knowledge can be understood in the category of ethnoscience. In terms of the UNESCO world heritage of intangible cultural assets, the knowledge is mainly about 'the knowledge on nature and universe'. Unique knowledge and skills are, however, identified in the molding step. They can be referred to 'body techniques', which unify the physical stance of potters, tools they employ, and the conceived pottery. Potters themselves find it difficult to articulate the knowledge. In case stated, it cannot be easily understood without the experience and knowledge on the field. From the preparation of raw material to the complete products, the techniques and traditional knowledge involved in the process of manufacturing pottery are closely connected, employing numerous categories and levels. Such an aspect can be referred to as a 'techniques chain'. Here the techniques mean not only the scientific techniques but also, in addition to the skills, the knowledge of various techniques and levels including habitual, unconscious behaviors of potters.

A Comparative Study on the Change in Oriental Linked pearls Pattern (동전(東傳) 연주문의 변천과정 비교연구 -5세기~10세기 벽화복식 및 출토 직물을 중심으로-)

  • An, Bo-yeon
    • Korean Journal of Heritage: History & Science
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    • v.40
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    • pp.243-270
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    • 2007
  • Linked pearls pattern expressed on textiles have no limited scale or shape when manufacturing, so they are free in expression. And from the design, material, and color we can analogize the social culture of that age. Oriental linked pearls pattern was started from the Sasanian Persia and introduced through the Silk Road, so it is closely connected with the East and the West culture. This study will consider from the 5th century to the 10th century; the mural costume of the West Central Asia, the ancient textiles excavated from the Sinjiang and Qinghai area of China, and the linked pearls pattern which are collected at Shosoin, Japan. And from this study, will concentrate on clarifying the linked pearls pattern's condition of the cultural exchange between the East and the West and it's structural variation process. The design of linked pearls pattern delivered to the East through the Silk Road is differed by area. For example, in the Western Pamir Plateau, where the ancient Sogdians mainly lived, the excavated linked pearls pattern's subject were deer or cassowary variated from the West Asian motif. But the ones excavated from Kucha Xingang had Chinese motifs added so they showed Chinese characters or Buddhist Bodhisattva image instead of Helios. Like this, the appearance of new patterns, which were accompanied by structural variations, gradually deviated from the standardized pattern of the Sasanian Persia. And this structural variation process has relations with the construction and arrangement method of various patterns of the after ages. The foliated floral Spray, which is placed at the lozenge space of linked pearls' space, had developed into ogival - shaped pattern (Neunghwamun). And the prevalence of geometrical structure pattern after the 10th century and the unfolding method of Tapjamun which is arranging unit pattern in order, are similar to the linked pearl pattern. In brief, linked pearls pattern accompanied by technical improvement let us understand the polished artistic code from its expression, and has importance in showing universal pattern beyond region and culture.

Changes in Marketability of Strawberry 'Maehyang' for Export as Affected by Concentration of Gaseous Chlorine Dioxide Treatment (이산화염소 가스 처리 농도에 따른 수출 딸기 '매향'의 상품성 변화)

  • Kim, Hye Min;Hwang, Seung Jae
    • Journal of Bio-Environment Control
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    • v.28 no.2
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    • pp.166-171
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    • 2019
  • This study was conducted to investigate the effect of gaseous chlorine dioxide ($ClO_2$) concentration and treatment method for maintaining marketability in strawberries 'Maehyang' for export. After harvesting strawberry colored with $60{\pm}5%$ of the skin, and the gaseous $ClO_2$ was applied as four treatments in the cold store set with $10^{\circ}C$ which were as follows: i) non-treatment (Control), ii) $0.2mg{\cdot}L^{-1}$ for 30 minutes, iii) $0.4mg{\cdot}L^{-1}$ for 30 minutes, and iv) continuously exposed at $0.4mg{\cdot}L^{-1}$ during the storage period. Weight loss, firmness, soluble solids content, color, incidence of gray mold, and quality grade of strawberries were investigated every 3 days during 16 days storage in cold store. The weight loss was consistently high at $0.2mg{\cdot}L^{-1}\;ClO_2$ treatment, and the weight loss was lower than other treatments when gaseous $ClO_2$ was continuously treated. Firmness was significantly higher at 0.2 and $0.4mg{\cdot}L^{-1}$ $ClO_2$ treatment on the 13th day of storage. Soluble solids content tended to below in continuous gaseous $ClO_2$ treatment. The colors showed no tendency as affected by concentrations and treatment methods of gaseous $ClO_2$. No gray mold was observed in the continuous gaseous $ClO_2$ treatment until the 13th day of storage. The quality grade was lower in gaseous $ClO_2$ treatment group than in the control. As a result, it was possible to decrease the weight loss and the incidence of gray mold by continuously treating the gaseous $ClO_2$ to strawberry 'Maehyang' for export during the storage period after harvest. However, further research is needed on method to maintain quality grades of fruits.

Application of Terrestrial LiDAR for Reconstructing 3D Images of Fault Trench Sites and Web-based Visualization Platform for Large Point Clouds (지상 라이다를 활용한 트렌치 단층 단면 3차원 영상 생성과 웹 기반 대용량 점군 자료 가시화 플랫폼 활용 사례)

  • Lee, Byung Woo;Kim, Seung-Sep
    • Economic and Environmental Geology
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    • v.54 no.2
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    • pp.177-186
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    • 2021
  • For disaster management and mitigation of earthquakes in Korea Peninsula, active fault investigation has been conducted for the past 5 years. In particular, investigation of sediment-covered active faults integrates geomorphological analysis on airborne LiDAR data, surface geological survey, and geophysical exploration, and unearths subsurface active faults by trench survey. However, the fault traces revealed by trench surveys are only available for investigation during a limited time and restored to the previous condition. Thus, the geological data describing the fault trench sites remain as the qualitative data in terms of research articles and reports. To extend the limitations due to temporal nature of geological studies, we utilized a terrestrial LiDAR to produce 3D point clouds for the fault trench sites and restored them in a digital space. The terrestrial LiDAR scanning was conducted at two trench sites located near the Yangsan Fault and acquired amplitude and reflectance from the surveyed area as well as color information by combining photogrammetry with the LiDAR system. The scanned data were merged to form the 3D point clouds having the average geometric error of 0.003 m, which exhibited the sufficient accuracy to restore the details of the surveyed trench sites. However, we found more post-processing on the scanned data would be necessary because the amplitudes and reflectances of the point clouds varied depending on the scan positions and the colors of the trench surfaces were captured differently depending on the light exposures available at the time. Such point clouds are pretty large in size and visualized through a limited set of softwares, which limits data sharing among researchers. As an alternative, we suggested Potree, an open-source web-based platform, to visualize the point clouds of the trench sites. In this study, as a result, we identified that terrestrial LiDAR data can be practical to increase reproducibility of geological field studies and easily accessible by researchers and students in Earth Sciences.

Provenance of the Sediments of the Araon Mound in the Chukchi Sea, Arctic Ocean (북극 척치해 아라온 마운드 퇴적물의 기원지에 관한 연구)

  • Jang, JeongKyu;Koo, HyoJin;Cho, HyenGoo
    • Korean Journal of Mineralogy and Petrology
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    • v.34 no.1
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    • pp.15-29
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    • 2021
  • In the Arctic Ocean, the distribution of sea ice and ice sheets changes as climate changes. Because the distribution of ice cover influences the mineral composition of marine sediments, studying marine sediments transported by sea ice or iceberg is very important to understand the global climate change. This study analyzes marine sediment samples collected from the Arctic Ocean and infers the provenance of the sediments to reconstruct the paleoenvironment changes of the western Arctic. The analyzed samples include four gravity cores collected from the Araon mound in the Chukchi Plateau and one gravity core collected from the slope between the Araon mounds. The core sediments were brown, gray, and greenish gray, each of which corresponds to the characteristic color of sediments deposited during the interglacial/glacial cycle in the western Arctic Ocean. We divide the core sediments into three units based on the analysis of bulk mineral composition, clay mineral composition, and Ice Rafted Debris (IRD) as well as comparison with previous study results. Unit 3 sediments, deposited during the last glacial maximum, were transported by sea ice and currents after the sediments of the Kolyma and Indigirka Rivers were deposited on the continental shelf of the East Siberian Sea. Unit 2 sediments, deposited during the deglacial period, were from the Kolyma and Indigirka Rivers flowing into the East Siberian Sea as well as from the Mackenzie River and the Canadian Archipelago flowing into the Beaufort Sea. Unit 2 sediments also contained an extensive amount of IRD, which originated from the melted Laurentide Ice Sheet. During the interglacial stage, fine-grained sediments of Unit 1 were transported by sea ice and currents from Northern Canada and the East Siberian Sea, but coarse-grained sediments were derived by sea ice from the Canadian Archipelago.