• ALGAE
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• v.25 no.4
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• pp.197-204
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• 2010

Cytoskeletal changes were observed during cell division of the green alga Zygnema cruciatum using flourescein isothiocynate (FITC)-conjugated phallacidin for F-actin staining and FITC-anti-$\alpha$-tubulin for microtubule staining. Z. cruciatum was uninucleate with two star-shaped chloroplasts. Nuclear division and cell plate formation occurred prior to chloroplast division. Actin filaments appeared on the chromosome and nuclear surface during prophase, and the F-actin ring appeared as the cleavage furrow developed. FITC-phallacidin revealed that actin filaments were attached to the chromosomes during metaphase. The F-actin ring disappeared at late metaphase. At telophase, FITC-phallacidin staining of actin filaments disappeared. FITC-anti-$\alpha$-tubulin staining revealed that microtubules were arranged beneath the protoplasm during interphase and then localized on the nuclear region at prophase, and that the mitotic spindle was formed during metaphase. The microtubules appeared between dividing chloroplasts. The results indicate that a coordination of actin filaments and microtubules might be necessary for nuclear division and chromosome movement in Z. cruciatum.

• Journal of fish pathology
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• v.14 no.1
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• pp.3-10
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• 2001

The present study was to obtain a basic research data about medicinal herbs by screening in vitro antimicrobial activity and the production of superoxide anion($O_2^-$) from the head kidney phagocytes of olive flounder, Paralichthys olivaceus. The following fourteen kinds of medicinal herbs extracted by boiling water were used : Gosam, Gwijeonu, Gujeolcho, Bagha, Bangpung, Yeongyo, Yagssug, Jiyu, Sambaegcho, Samjiguyeobcho, Sangbaegpi, Sohwehyang, Pyeonchug, Palgag. Antimicrobial activity against fish pathogenic bacteria, Listonella anguillarum, Vibrio sp., Vibrio alginolyticus, Edwardsiella tarda, Streptococcus sp. and Lactococcus garvieae, and the production of superoxide in kidney macrophage of olive flounder were examined by disk method and nitroblue tetrazolium(NBT) reaction, respectively. Among the tested herbs, Yagssug showed the highest antimicrobial activity against those fish pathogenic bacteria and stimulation of $O_2^-$ production.

• Proceedings of KOSOMES biannual meeting
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• 2003.11a
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• pp.175-180
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• 2003

Water temperature data of National. Fisheries Research and Development Institute (NFRDI) during periods of 19m to 2002 were analyzed to investigate the effect of the water temperature on catches of anchovy and laver in the eastern part of the Southern Waters of Korea The annual catches of anchovy and laver increased gradually although they showed fluctuations each year. Our findings indicated tint the catches cf anchovy and laver were controlled by various oceanographic conditions, mainly water temperature. The appearance of cooler sea surface water, warmer water at 0 to 20 m depth, and bottom water of about $15^{\circ}C$ caused relatively lower catches.

• Journal of Environmental Science International
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• v.19 no.3
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• pp.281-293
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• 2010

The present study explores time and spatial thermal environment for Daegu, which is a city built on a basin area, according to varying land cover conditions of the earth's surface by analyzing data derived from meteorological observation and satellite images. The study has classified land use by utilizing MODIS satellite images and analyzed land surface temperature. Also, by using data acquired from automatic weather system, the study has evaluated the effects of atmospheric heating caused by city pavements by analyzing the sensible heat flux between the city's land surface and the atmosphere. The results are as follows. 1) Classification of land use in the Daegu area shows 46.64% of urban and built-up area, 1.39% of watersides, 35.19% of forest, 11.43% of crops, and 5.37% grasslands. 2) During the weekdays throughout the year, the land surface temperature was high for Dalseogu, Bukgu, and Seogu regions where industrial complexes could be found. Comparatively, lower temperature could be observed in the woodlands. 3) While the land surface temperature displayed the effects of pushing air upwards during the weekdays in urban areas, the reverse was true for forest regions. During the night, the temperature did not exert any significant influence on air movement.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.51 no.2
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• pp.212-220
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• 2015

We examined the appearance of cold water in the southwest region of the East Sea, based on the sea surface temperature (SST) at the east coast of Korea and buoy data in Donghae ($37^{\circ}31$'N, $130^{\circ}00$'E, 80 km east away from Donghae port) and Pohang ($36^{\circ}21$'N, $129^{\circ}46$'E, 35 km east away from Ganggu port) from June to August in 2013. Also, the serial oceanographic data of National Fisheries Research and Development Institute (NFRDI) were used to see the oceanographic conditions for June and August in 2013. The SST anomaly at the east coast showed negative values in $3{\sim}6^{\circ}C$ from 2 July. At Janggigab, the SST anomaly showed negative value amount to $10^{\circ}C$ in 8 July. The negative values of SST anomaly continued to the middle of August at Janggigab. The wind speed was 6~11 m/s and the direction was south-southwestly in 1 July. The wind speed amounts to 6~16 m/s in 2 July. It means that the strong wind induced the upwelling effect by a day. The temperature was lower than normal at the depth in 20 m of the East Sea in June and August. The air pressure was 996~998 hPa in the beginning of July. It was the lowest air pressure during the studied period. The correlation was 0.3 between the SST anomaly and air pressure. It was suggested that the appearance of cold water in the East Sea was influenced by a stirring due to wind and low air pressure as well as coastal upwelling.

• Journal of fish pathology
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• v.23 no.1
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• pp.137-143
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• 2010

Hot water extracts of 5 herbs were tested in-vitro for anti-bacterial, anti-fungal, anti-parasitical effects for possible use against fish diseases. Skullcap, Scutellaria baicalensis, was the most effective herb in these 5. The effects of skullcap in cultured flounder were examined for various physiological responses and bacterial disease-prevention follow as feeding skullcap absorbed diet for 4, 8, 12weeks. There were not any significant effects in physiological responses, except beneficial action of growth promotion. No definitive preventive activity was observed with experimental feeding of the extract against infected flounder. As we could not confirm in-vivo antibacterial effects of skullcap in flounder despite its in-vitro efficacy, further studies are needed to define the in-vivo efficacy.

• The Korean Journal of Malacology
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• v.23 no.1
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• pp.1-8
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• 2007

The influence of individual size, sediment, gain size, water temperature, salinity and air exposure on burrowing rate was investigated in order to obtain the basic biological data on applying shellfish farm for a sustainable production of ark shell, Scapharca broughtonii (Schrenk). The burrowing rate on individual size 300 minutes after starting the experiment was the highest in the shell length $16.3\;{\pm}\;1.2\;mm$, 97.7%. The highest burrowing rates were 97.0% in $12.8\;{\pm}\;0.8\;mm$, 96.7% in $9.2\;{\pm}\;1.0\;mm$, and 96.3% in $5.9\;{\pm}\;0.7\;mm$. The clams over 6 mm of shell length had burrowing ability and the burrowing rate was not related to the shell size. The burrowing rate depending on the kind of grain at the bottom after 300 minutes was the highest, 98.3%, in the mixture of sand and silt with a ratio of 75:25. The rates were 98% in silt (100%), 97.3% in mixture sand and silt with a ratio of 50:50, 97.3% in sand and silt ratio of 25:75, and 86.3% in sand (100%) in this specific order. On grain size of the soil in the seafloor, the burrowing rates after 300 minutes was at its highest in the group of sand in pore size 1 mm with 85.0%, and the $12\;{\mu}m$ to 1 mm in the grain size was fitted to burrowing of artificial seed. In the case of water temperature, the burrowing rates were at its highest after 300 minutes. In $30^{\circ}C$ group, the rate was 96.7% and in $25^{\circ}C$ and $20^{\circ}C$, 90.0%. The rates decreased as the water temperature decreased below $15^{\circ}C$. The burrowing rates on salinity were the highest in 30 psu with 93.3% and at 15 psu and below, there was no noticeable change in the burrowing rate. On air exposure, the burrowing rates after 300 minutes were the highest in 1 hour with 93.3%, and remarkably decreased as air exposure time is longer after 12 hours of air exposure.

• Journal of fish pathology
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• v.25 no.2
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• pp.67-76
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• 2012

Two bacteria strains were isolated from emaciated olive flounder (Paralichthys olivaceus) in aquafarm and were identificated as Vibrio harveyi (JV1) and Edwardsiella tarda (JE1), respectively. In the challenge experiments, we found 100% cumulative mortalities in all of olive flounder injected with JV1, JE1 or JV1+JE1 within eleven days after the injection. Two bacteria strains were reisolated from dead fish and were analyzed using the PCR method. In the physilogical analysis, the hematocrit, AST, ALT and cholesterol levels in experimental groups were increased significantly compared to those in control group, but the glucose, total protein and triglyceride levels were significantly decreased. Additionally, the lysozyme activity in the blood serum was decreased. The histopathological observations of the intestine showed that all groups had detachment and destruction of epithelial tissues except for the control group.

• Proceedings of KOSOMES biannual meeting
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• 2007.05a
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• pp.43-50
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• 2007

In order to calculate the strength and to. see the variation af the stratification in the Southern Waters af Korea, the stratification parameter defined as potential energy anomaly (PEA, $V(J/m^3)$) introduced by Simpson and Hunter (1974) was used The data used in this paper were observed in August 1999 and February 2000 by National Fisheries Research and Development Institute (NFRDI). Also to know the effects af the temperature and the salinity an the stratification respectively, averaged temperature and salinity were used in the process af calculation the parameter. V is generally high in the offshore. However, in February, V in the onshore is higher than that of the offshore due to the vertical temperature gradient caused by the expansion of South Korean Coastal Waters (SKCW). In the summer, the increase af the atmospheric heating, the temperature inversion phenomenon act an the stratification as the buoyancy forcing. In most cases, the effects of the temperature on the stratification is stronger than that of the salinity. The temperature effect is predominantly due to the extent af the intrusion of Tsushima Warm Current into the study area. However, at stations where V is high the effect af the salinity is also significant. In the winter, V is very low due to the decrease of the buoyancy forcing, but same stations show the relatively high V due to the expansion of SKCW and Tsushima Warm Current.

• Journal of fish pathology
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• v.23 no.1
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• pp.27-35
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• 2010

Pathogens on the cultured 579 rockfish, Sebastes schlegeli in the marine cage farms from Geoje and Tongyeong of the Southern sea were investigated from 2006 to 2008. The pathogens were detected throughout the year at 46.0~90.0% for 3 years and the detection rate was low with an average 58.1% in May and high with an average 81.5% in October. Bacteria only, bacteria-parasite mix and virus only were found in October and November as well as parasite only, whereas infection of parasite only was dominant in May when the temperature increased and in August when the temperature peaked. Of rockfish, Microcotyle sp. and Caligus sp. were dominant for parasitic disease, and Vibrio sp. and Streptococcus sp. were dominant bacteria. For virus, RSIV and VNNV were detected as dominant organisms. While no virus was detected in 2006, VNNV, VHSV and RSIV were detected in 2007 due to $1.5\sim2.0^{\circ}C$ higher temperature than 2006 in the summer season. For total prevalence by rockfish sizes, the highest was found at 50.0~87.1% in 11~15cm sizes and 50% was found in 30 cm size. Parasite showed a similar trend of 50.0~79.6% as the total prevalence. Prevalence for bacteria varied from 1.6% (for 10 cm group) to 23.8% (for 26 cm group) and higher virus prevalence of 21.5% was found from below 25 cm group.