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http://dx.doi.org/10.7847/jfp.2012.25.2.067

Clinical features of fish with pathogens isolated from emaciated olive flounder Paralichthys olivaceus  

Choi, Hye-Sung (Pathology Division, National Fisheries Research and Development Institute (NFRDI))
Jun, Lyu-Jin (School of Marine Biomedical Sciences, College of Ocean Science & Marine and Environmental Research Institute, Jeju National University)
Kim, Seoung-Min (School of Marine Biomedical Sciences, College of Ocean Science & Marine and Environmental Research Institute, Jeju National University)
Jeong, Hyun-Do (Department of Aquatic Life Medicine, Pukyong National University)
Kim, Yi-Kyung (Department of Aquatic Life Medicine, Pukyong National University)
Lim, Hee-Young (Institute for Clinical Pharmacology, Medical Center of Goethe University)
Yeo, In-Kyu (School of Marine Biomedical Sciences, College of Ocean Science & Marine and Environmental Research Institute, Jeju National University)
Jeong, Joon-Bum (School of Marine Biomedical Sciences, College of Ocean Science & Marine and Environmental Research Institute, Jeju National University)
Publication Information
Journal of fish pathology / v.25, no.2, 2012 , pp. 67-76 More about this Journal
Abstract
Two bacteria strains were isolated from emaciated olive flounder (Paralichthys olivaceus) in aquafarm and were identificated as Vibrio harveyi (JV1) and Edwardsiella tarda (JE1), respectively. In the challenge experiments, we found 100% cumulative mortalities in all of olive flounder injected with JV1, JE1 or JV1+JE1 within eleven days after the injection. Two bacteria strains were reisolated from dead fish and were analyzed using the PCR method. In the physilogical analysis, the hematocrit, AST, ALT and cholesterol levels in experimental groups were increased significantly compared to those in control group, but the glucose, total protein and triglyceride levels were significantly decreased. Additionally, the lysozyme activity in the blood serum was decreased. The histopathological observations of the intestine showed that all groups had detachment and destruction of epithelial tissues except for the control group.
Keywords
Olive flounder; Vibrio harveyi; Edwardsiella tarda;
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Times Cited By KSCI : 4  (Citation Analysis)
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