Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
권호 표지

Anti-fish pathogenic efficacy of hot water extracts obtained from 5 herbs in-vitro, and efficacy and toxicity in flounder of the one selected herb, skullcap

생약재 5종 열수추출물의 어류 병원체에 대한 in-vitro 약효와 선택된 한 종(황금)의 넙치에서의 항균효능과 독성시험

  • Lee, Nam-Sil (Division of Pathology, National Fisheries Research and Development Institute) ;
  • Jeong, Sung-Hee (Division of Pathology, National Fisheries Research and Development Institute) ;
  • Jee, Bo-Young (Aquatic Life Disease Control Division, NFRDI)
  • 이남실 (국립수산과학원 병리연구과) ;
  • 정승희 (국립수산과학원 병리연구과) ;
  • 지보영 (국립수산과학원 수산생물방역과)
  • Received : 2010.02.28
  • Accepted : 2010.04.07
  • Published : 2010.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

Hot water extracts of 5 herbs were tested in-vitro for anti-bacterial, anti-fungal, anti-parasitical effects for possible use against fish diseases. Skullcap, Scutellaria baicalensis, was the most effective herb in these 5. The effects of skullcap in cultured flounder were examined for various physiological responses and bacterial disease-prevention follow as feeding skullcap absorbed diet for 4, 8, 12weeks. There were not any significant effects in physiological responses, except beneficial action of growth promotion. No definitive preventive activity was observed with experimental feeding of the extract against infected flounder. As we could not confirm in-vivo antibacterial effects of skullcap in flounder despite its in-vitro efficacy, further studies are needed to define the in-vivo efficacy.

5가지 생약재로부터 얻어낸 열수추출물을 대상으로 시험관(in-vitro) 실험을 통하여 어류병원체에 대한 항세균, 항곰팡이, 항기생충 효과를 검토하였다. 황금의 효과가 가장 두드러졌으며, 황금의 열수추출물을 사료에 흡수시켜 넙치에 급여하고 4, 8, 12주 후에 다양한 생리학적 반응과 세균성질병(Edwardsiellosis, Streptococosis)에 대한 예방효과를 조사한 결과, 성장효과에 대한 변화 이외의 뚜렷한 생리학적변화는 나타나지 않았으며, 두 가지 세균성 질병에 대한 예방효과도 확인되지 않았다. 따라서 이 후 추가적인 조사 연구가 이루어져야 할 것으로 생각된다.

Keywords

References

  1. Choi H.S., Kim J.W., Jang D.S., Yu Y.B., Kim Y.C. and Lee J.S.: Antibacterial activities of Galla rhois extracts against fish pathogenic bacteria. J. Fish Pathol., 18:239-246, 2005a.
  2. Choi H.S., Kim Y.C., Lee J.S., Jo M.R., Seo C.H. and Park S.L.: Antibacterial activities of hotwater and ethyl alcohol extracts of medicinal herbs on fish pathogenic bacteria. J. Fish Pathol., 17:39-55, 2004.
  3. Choi M.S., Park S.W. and Park K.H.: Effect of Red ginseng extract on immune function of Israeli carp. Cyprinus carpio. J. Fish Pathol., 18:277-286, 2005b.
  4. Chou T.C., Chang L.P. Li C.Y., Wong C.S. and Yang S.P.: The antiinflammatory and analogesic effects of baicalin in carrageenanevoked thermal hyperalgesia. Anesthesia and Analgesia, 97:1724-1729, 2003. https://doi.org/10.1213/01.ANE.0000087066.71572.3F
  5. Jang S.I., Kim H.J., Hwang K.M., Jekal S.J., Pae H.O., Choi B.M., Yun Y.G., Kwon T.O., Chung H.T. and Kim Y.C.: Hepatoprotective effect of baicalin, a major flavone from Scutellaria radix, on acetaminophen-induced liver injury in mice. Immunopharmacol Immunotoxicol, 25:585-594, 2003. https://doi.org/10.1081/IPH-120026443
  6. Jee B.Y., Lee N-.S., Kim J.W. Park M.S. and Jeong S.H.: The preventive effects of hydrolysis compound of cactus opuntia genus, Opuntia ficus-indica var. saboten Makino against bacterial disease on cultued flounder, Paralichthys olivaceus. J. Fish Pathol., 20:179-187, 2007.
  7. Jo, M.R., Kim J.W. and Kim D.S.: Antimicrobial effects of natural plant and mushroom, Dicyphora indusiata extracts on fish pathogenic bacteria. J. Kor. Fish Soc., 35:578-582, 2002.
  8. Jung S.H., Lee N-.S., Jee B.Y. and Park M.S.: Antimicrobial activity of hydrolysis compound of cactus stem(Opuntia ficus-indica var. saboten Makino) against fish pathogenic bacteria. J. Fish Pathol., 21:29-34, 2008.
  9. Jung, S.H., Shon Y.C. and Kim Y.C.: In vitro effect of water extract of medicinal herbs on antimicrobial activity against fish pathogenic bacteria and superoxide production of kidney phagocytes in olive flounder, Paralichthys olivaceus. J. Fish Pathol., 14:3-10, 2001.
  10. Kang S.Y.: The antimicrobial compound of Thus verniciflua barks against fish pathogenic gram-negative bacteria Edwardsiella tarda and Vibrio anguillarum. J. Fish Pathol., 18:227-238, 2005.
  11. Kim Y.G., Rho B.J. and Lee K.K.: Antimicrobial activity of Artemisia princeps var. orientalis essential oil against fish pathogenic bacteria. J. Fish Pathol., 7:105-112, 1994.
  12. Lang S., Gudmundsdottir B.K. and Magnadottir, B.: Humoral immune parameters of cultured Atlantic halibut (Hippoglossus hippoglossus L.). Fish Shellfish Immunol., 11:523-535, 2001. https://doi.org/10.1006/fsim.2000.0333
  13. Shimizu L, Ma Y.R., Mizobchi Y., Liu F., Miura T., Makai Y., Yasuda M., Shiba M., Horie T., Amagaya S., Kawada N., Hori H. and Ito S.:Effects of Sho-saiko-to, a Japanese herbal medicine, on hepatic fibrosis in rats. Hepatology, 29:149-160, 1999. https://doi.org/10.1002/hep.510290108
  14. Taira Z., Yabe K., Hamaguchi Y., Hirayama K., Kishimoto M., Ishida S. and Ueda Y.: Effects of Sho-saiko-to extraxt and its components, Baicalin, baicalein, glycyrrhizin and glycyrrhetic acid, on pharmacokinetic behavior of salicylamide in carbon tetrachloride intoxicated rats. Food Chem. Toxicol., 42:803-807, 2004. https://doi.org/10.1016/j.fct.2003.12.017