• Proceedings of the KIEE Conference
• /
• 2008.10b
• /
• pp.280-281
• /
• 2008

본 논문은 큰 구조물의 3파인 영상 재구성을 위해서 획득한 2차원 영상에서 특징점을 찾아 선으로 조합한 후 선 길이 정보를 추출하는 방법을 제안한다. 거대한 구조물의 외곽선 길이 정보 추출을 위해서는 광각 카메라에 의한 영상을 획득한다. 영상에서의 외곽선들은 모델의 기울어진 정보와 형태, 모델의 크기 등을 결정하게 되는데 광각카메라 사용에 의하여 배럴왜곡, 원근투영왜곡 등이 발생한다. 외곽선 정보 추출의 순서는 먼저모델의 2차원영상을 획득하고 이로부터 왜곡이 보정된 그레이영상을 획득한다. 이 그레이영상에서 잡음을 제거하고 특징점을 찾기 위하여 SUSAN 알고리즘을 사용한다. SUSAN알고리즘 기법은 적은 계산량과 잡음에 매우 강한 장점이 있어서 영상에서의 특징점을 얻기 위한 효과적인 기법이다. 특징점을 3차원 벡터공간에서 맵핑시킨 후 X, Y, Z 좌표축으로 점과 선으로 나타내고 시작점과 끝점의 좌표를 이용하여 벡터 길이를 얻는다. 이러한 벡터 데이터와 3차원 영상 재구성을 위한 라이브러리인 OpenGL을 사용하여 3차원 공간에 거대한 구조물들을 재구성하는 소프트웨어를 개발하였다.

• The Transactions of the Korean Institute of Electrical Engineers D
• /
• v.53 no.10
• /
• pp.703-713
• /
• 2004

This paper presents the new obstacle avoidance method that is composed of vision and sonar sensors, also a navigation algorithm is proposed. Sonar sensors provide poor information because the angular resolution of each sonar sensor is not exact. So they are not suitable to detect relative direction of obstacles. In addition, it is not easy to detect the obstacle by vision sensors because of an image disturbance. In This paper, the new obstacle direction measurement method that is composed of sonar sensors for exact distance information and vision sensors for abundance information. The modified splitting/merging algorithm is proposed, and it is robuster for an image disturbance than the edge detecting algorithm, and it is efficient for grouping of the obstacle. In order to verify our proposed algorithm, we compare the proposed algorithm with the edge detecting algorithm via experiments. The direction of obstacle and the relative distance are used for the inputs of the fuzzy controller. We design the angular velocity controllers for obstacle avoidance and for navigation to center in corridor, respectively. In order to verify stability and effectiveness of our proposed method, it is apply to a vision and sonar based mobile robot navigation system.

• Journal of the Korean Magnetic Resonance Society
• /
• v.9 no.2
• /
• pp.103-109
• /
• 2005

New PVC plasticizer, 2,2,4-trimethyl-1,3-pentanediol-1-butyrate-3-isobutyrate was synthesized via simple esterification with butyric acid and Texanol(a trademark of Eastman Chemicals), the mixture of 2,2,4-trimethyl-1,3-pentanediol-3-isobutyrate and 2,2,4-trimethyl-1,3-pentanediol-1-isobutyrate. The analysis of $^1H-1D,\;^{13}C-1D$ NMR and HMBC spectra identified internal-ester-transfer of 2,2,4-trimethyl-1,3-pentanediol-1-isobutyrate during the reaction. 2,2,4-trimethyl-1,3-pentanediol-1-butyrate-3-isobutyrate gave better properties in PVC than 2,2,4-trimethyl-1,3-pentanediol diisobutyrate(TXIB, a trademark of Eastman Chemicals) such as lower viscosity, higher tensile strength and better elongation. In particular, remarkably reduced migration compared with TXIB suggested a reduced emission of VOC(volatile organic compound) from PVC.

• The Korean Journal of Food And Nutrition
• /
• v.26 no.3
• /
• pp.485-491
• /
• 2013

Rhodiola spp. and red ginseng have been used for food and medicinal applications in disease chemoprevention in many Asian countries. Increased oxidative stress by reactive oxygen species (ROS) has been proposed to be a major cause of muscle fatigue. The present study was designed to investigate the protective effects of a fermented hot-water extract mixture from Rhodiola sachalinensis and red ginseng (MFR) on cell damage and the antioxidant enzyme system in $H_2O_2$-induced oxidative stress in skeletal muscle cells. C2C12 myoblasts were treated with various concentrations of NFR (non-fermented Rhodiola sachalinensis extract), FR (fermented hot-water extract from Rhodiola sachalinensis) and MFR for up to 5 days after the standard induction of differentiation, followed by semi-quantitative RT-PCR. MFR treatment dose-dependently protected oxidative damage of C2C12 cells. The treatment with MFR also enhanced mRNA expressions of MyoD, Cu/Zn SOD, Mn-SOD and GPX up to 16%. These results indicate that MFR exerts an anti-oxidative effect through a mechanism (s) that may involve the up-regulation of antioxidant enzymes, which may be important for the cellular redox environment in muscle cells.

• The Korea Journal of Herbology
• /
• v.37 no.4
• /
• pp.31-38
• /
• 2022

Objectives : In this study, we investigated the synergistic protective effects of medicinal herbal mixture (HME) including Mori Ramulus (MR), Acanthopanacis Cortex (AC), Eucommiae Cortex (EC), and Black soybean (BS) in C2C12 cells, mouse myoblasts. Methods : Effects of HME on cell viability of C2C12 myoblasts were monitored by MTT assay. Anti-atrophic activity of HME was determined in myoblasts and myotubes under oxidative stress by H₂O₂. C2C12 myoblasts were differentiated into myotubes in a medium containing 2% horse serum for 6 days. After that, we measured that expression of MyoD and myogenine, the myogenic regulatory factors, to identify the mechanism of inhibiting muscle atophy after HME treatment. In addition, suppression of phosphorylation of Akt, FoxO3a and MARF-1, transcription factors of degradation proteins were analyzed via western blotting. Results : As a result of MTT, HME there was no show cytotoxicity up to a concentration of 1 mg/ml. The cytoprotective effects on oxidative stressed myoblast and myotube was better in HME extract than those of MR, AC, EU, and BS, respectively. HME treatment in Myotube induced by oxidative stress after H₂O₂ treatment increased Myo D, Myogenine activation, and Akt, FoxO3a phosphorylation and decreased expression of MuRF-1. As the results, HME has synergistic effects on protection against proteolysis of C2C12 myotubes through activation of the Akt signaling pathway under oxidative stress. Conclusions : These results suggest that HME may also be useful as a preventing and treating material for skeletal muscle atrophy caused by age-related diseases.

• Journal of Ginseng Research
• /
• v.47 no.6
• /
• pp.726-734
• /
• 2023

Background: Skeletal muscles play a key role in physical activity and energy metabolism. The loss of skeletal muscle mass can cause problems related to metabolism and physical activity. Studies are being conducted to prevent such diseases by increasing the mass and regeneration capacity of muscles. Ginsenoside Rg5 has been reported to exhibit a broad range of pharmacological activities. However, studies on the effects of Rg5 on muscle differentiation and growth are scarce. Methods: To investigate the effects of Rg5 on myogenesis, C2C12 myoblasts were induced to differentiate with Rg5, followed by immunoblotting, immunostaining, and qRT-PCR for myogenic markers and promyogenic signaling (p38MAPK). Immunoprecipitation confirmed that Rg5 increased the interaction between MyoD and E2A via p38MAPK. To investigate the effects of Rg5 on prevention of muscle mass loss, C2C12 myotubes were treated with dexamethasone to induce muscle atrophy. Immunoblotting, immunostaining, and qRT-PCR were performed for myogenic markers, Akt/mTOR signaling for protein synthesis, and atrophy-related genes (Atrogin-1 and MuRF1). Results: Rg5 promoted C2C12 myoblast differentiation through phosphorylation of p38MAPK and MyoD/E2A heterodimerization. Furthermore, Rg5 stimulated C2C12 myotube hypertrophy via phosphorylation of Akt/mTOR. Phosphorylation of Akt induces FoxO3a phosphorylation, which reduces the expression of Atrogin-1 and MuRF1. Conclusion: This study provides an understanding of how Rg5 promotes myogenesis and hypertrophy and prevents dexamethasone-induced muscle atrophy. The study is the first, to the best of our knowledge, to show that Rg5 promotes muscle regeneration and to suggest that Rg5 can be used for therapeutic intervention of muscle weakness and atrophy, including cancer cachexia.

• Journal of Applied Biological Chemistry
• /
• v.66
• /
• pp.60-66
• /
• 2023

Lentinula edodes is one of the most produced mushrooms in the world. In this study, the effects of L. edodes water extracts and lentinan, a beta-glucan from this mushroom, on the proliferation of Bos taurus Hanwoo myosatellite cells were studied. The betaglucan content of the L. edodes water extract was approximately 15.20% at 85 ℃ for 4 h, 13.64% at 100 ℃ for 4 h, 9.48% at 40 ℃ for 8 h and 8.21% at room temperature for 24 h. L. edodes water extract was added to the culture of Hanwoo myosatellite cells. The expression of the MyoD gene increased in the addition of the extract at 40 ℃ for 8 h and 100 ℃ for 4 h, and the expression of the Myogenin gene increased in the addition of the extract at 40 ℃ for 8 h, but proliferation and activity did not increase compared to no addition. However, the addition of lentinan to the culture of Hanwoo myosatellite cells increased the expression of Myogenin gene related to muscle formation increased and the proliferation and viability of the cells. This study proved that the components of L. edodes can affect the proliferation of Hanwoo myosatellite cells, and further research will help develop the mushroom industry and cultured meat industry in the future.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
• /
• v.18 no.4 s.119
• /
• pp.358-363
• /
• 2007

The multi-band and wide-band antenna for a portable broadcasting terminal is proposed. The proposed antenna consists of two radiators with a parallel structure. The antenna has an enough wide impedance bandwidth for the DVB-H(Digital Video Broadcasting-Handheld) service band since two radiators have adjacent resonance frequencies and operates in the DAB(Digital Audio Broadcasting) service band using the third harmonic of the radiator 1. The fabricated antenna has VSWR characteristics of less than 2:1 in the frequency band $470{\sim}740\;MHz$ for DVB-H and $1,450{\sim}1,480\;MHz$ for DAB. The measured peak gain of the antenna is $1.97{\sim}4.10\;dBi$ in the DVB-H band and $1.98{\sim}2.04\;dBi$ in the DAB band.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
• /
• v.20 no.2
• /
• pp.161-166
• /
• 2009

In this paper, we propose the internal mobile antenna for TDMB and KPCS. The proposed antenna is made of different dielectric substrate and it has small size ($45{\times}8{\times}8\;mm$, about 2.8 cc) for mobile device. TDMB antenna is designed spiral structure that makes maximum current for each cell and KPCS antenna is PIFA that is usually used for internal antenna. In order to compensate length of resonance TDMB antenna has a large inductor above 100 nH. In this case, the inductor isolate KPCS signal at TDMB by cutting high frequency. Also the antenna has good isolation because TDMB radiator is parasitic element in KPCS band. We simulated the antenna by using CST microwave studio and measured performance of the antenna in anechoic chamber Proposed antenna has $-6{\sim}-14\;dBi$ gain for TDMB and $-3.5{\sim}-5\;dBi$ gain for KPCS.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.31 no.3
• /
• pp.229-234
• /
• 2011

In order to develop an efficient, reliable and reproducible tissue culture system for reed (Phragmites communis Trinius), an efficient plant regeneration system via callus induction was established using mature seeds as explants. MS medium containing 1 mg/L 2,4-D and 0.5 mg/L BA was optimal for callus induction from mature seeds. The highest frequency (88.7%) of callus formation was obtained in 1.0 mg/L 2,4-D. The highest plant regeneration frequency (59.6%) was found when the embryogenic calli were subcultured on MS medium supplemented with 100 mg/L myo-inositol, whereas, adding of plant growth regulators was not so promising in this case. Our result would be useful for development of transgenic reed plants.