Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Effect of Plant Growth Regulators on Callus Induction and Plant Regeneration from Seed Culture of Reed

갈대(Phragmites communis Trinius.)의 종자배양에 있어서 식물생장조절물질이 캘러스 유도와 식물체 재분화에 미치는 영향

  • Kim, Yong-Goo (Dept. of Animal Biosciences, IALS, PMBBRC, Division of Applied Life Sciences BK21, Gyeongsang National University) ;
  • Kim, Kyung-Hee (Dept. of Animal Biosciences, IALS, PMBBRC, Division of Applied Life Sciences BK21, Gyeongsang National University) ;
  • Lee, Byung-Hyun (Dept. of Animal Biosciences, IALS, PMBBRC, Division of Applied Life Sciences BK21, Gyeongsang National University)
  • 김용구 (경상대학교 응용생명과학부 동물생명과학과) ;
  • 김경희 (경상대학교 응용생명과학부 동물생명과학과) ;
  • 이병현 (경상대학교 응용생명과학부 동물생명과학과)
  • Received : 2011.07.06
  • Accepted : 2011.08.08
  • Published : 2011.09.30
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Abstract

In order to develop an efficient, reliable and reproducible tissue culture system for reed (Phragmites communis Trinius), an efficient plant regeneration system via callus induction was established using mature seeds as explants. MS medium containing 1 mg/L 2,4-D and 0.5 mg/L BA was optimal for callus induction from mature seeds. The highest frequency (88.7%) of callus formation was obtained in 1.0 mg/L 2,4-D. The highest plant regeneration frequency (59.6%) was found when the embryogenic calli were subcultured on MS medium supplemented with 100 mg/L myo-inositol, whereas, adding of plant growth regulators was not so promising in this case. Our result would be useful for development of transgenic reed plants.

갈대의 성숙종자로부터 최적 조직배양조건을 확립하기 위하여 종자로부터 캘러스 유도 및 식물체 재분화 체계를 확립하였다. 종자로부터 캘러스 유도시 첨가되는 auxin으로는 2,4-D가 가장 효율적이었으며, 1.0 mg/L 2,4-D와 0.5 mg/L BA가 첨가된 MS 배지에서 가장 높은 빈도로 캘러스가 유도되었다. 캘러스로부터 식물체 재분화는 cytokinin류인 BA와 kinetin이 첨가되지 않은 MS 배지에서 59.6%로 높은 재분화 효율을 나타내었다. 본 연구를 통하여 확립된 성숙종자로부터의 고효율 재분화 시스템은 유전자 형질전환기술을 이용한 신품종 갈대 개발에 유용하게 이용 될 수 있을 것이다.

Keywords

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  1. Trinius) using mature seed-derived calli vol.5, pp.1, 2012, https://doi.org/10.1111/j.1757-1707.2012.01200.x
  2. Plant Regeneration and Genetic Diversity of Regenerants from Seed-derived Callus of Reed (Phragmites communis Trinius) vol.26, pp.2, 2013, https://doi.org/10.7732/kjpr.2013.26.2.320