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http://dx.doi.org/10.5333/KGFS.2011.31.3.229

Effect of Plant Growth Regulators on Callus Induction and Plant Regeneration from Seed Culture of Reed  

Kim, Yong-Goo (Dept. of Animal Biosciences, IALS, PMBBRC, Division of Applied Life Sciences BK21, Gyeongsang National University)
Kim, Kyung-Hee (Dept. of Animal Biosciences, IALS, PMBBRC, Division of Applied Life Sciences BK21, Gyeongsang National University)
Lee, Byung-Hyun (Dept. of Animal Biosciences, IALS, PMBBRC, Division of Applied Life Sciences BK21, Gyeongsang National University)
Publication Information
Journal of The Korean Society of Grassland and Forage Science / v.31, no.3, 2011 , pp. 229-234 More about this Journal
Abstract
In order to develop an efficient, reliable and reproducible tissue culture system for reed (Phragmites communis Trinius), an efficient plant regeneration system via callus induction was established using mature seeds as explants. MS medium containing 1 mg/L 2,4-D and 0.5 mg/L BA was optimal for callus induction from mature seeds. The highest frequency (88.7%) of callus formation was obtained in 1.0 mg/L 2,4-D. The highest plant regeneration frequency (59.6%) was found when the embryogenic calli were subcultured on MS medium supplemented with 100 mg/L myo-inositol, whereas, adding of plant growth regulators was not so promising in this case. Our result would be useful for development of transgenic reed plants.
Keywords
Callus; Plant regeneration; Reed; Mature seed;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
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