The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Protective Effects of Medicinal Herbal Mixture (HME) through Akt/FoxO3 Signal Regulation in Oxidative Damaged C2C12 Myotubes

C2C12 myotube의 산화적 손상에 대한 혼합 한약재 추출물(HME)의 Akt/FoxO3 신호 조절을 통한 보호 효과

  • Kim, So Young (Department of Herbal Pharmacology, College of Korean Medicine, Daegu Haany University) ;
  • Choi, Moon-Yeol (Department of Herbal Pharmacology, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Un Tak (Ebiche co., Ltd.) ;
  • Choo, Sung Tae (Ebiche co., Ltd.) ;
  • Kim, Mi Ryeo (Department of Herbal Pharmacology, College of Korean Medicine, Daegu Haany University)
  • 김소영 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 최문열 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 이은탁 (농업회사법인 (주)이비채) ;
  • 추성태 (농업회사법인 (주)이비채) ;
  • 김미려 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2022.06.14
  • Accepted : 2022.07.25
  • Published : 2022.07.30
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Abstract

Objectives : In this study, we investigated the synergistic protective effects of medicinal herbal mixture (HME) including Mori Ramulus (MR), Acanthopanacis Cortex (AC), Eucommiae Cortex (EC), and Black soybean (BS) in C2C12 cells, mouse myoblasts. Methods : Effects of HME on cell viability of C2C12 myoblasts were monitored by MTT assay. Anti-atrophic activity of HME was determined in myoblasts and myotubes under oxidative stress by H2O2. C2C12 myoblasts were differentiated into myotubes in a medium containing 2% horse serum for 6 days. After that, we measured that expression of MyoD and myogenine, the myogenic regulatory factors, to identify the mechanism of inhibiting muscle atophy after HME treatment. In addition, suppression of phosphorylation of Akt, FoxO3a and MARF-1, transcription factors of degradation proteins were analyzed via western blotting. Results : As a result of MTT, HME there was no show cytotoxicity up to a concentration of 1 mg/ml. The cytoprotective effects on oxidative stressed myoblast and myotube was better in HME extract than those of MR, AC, EU, and BS, respectively. HME treatment in Myotube induced by oxidative stress after H2O2 treatment increased Myo D, Myogenine activation, and Akt, FoxO3a phosphorylation and decreased expression of MuRF-1. As the results, HME has synergistic effects on protection against proteolysis of C2C12 myotubes through activation of the Akt signaling pathway under oxidative stress. Conclusions : These results suggest that HME may also be useful as a preventing and treating material for skeletal muscle atrophy caused by age-related diseases.

Keywords

Acknowledgement

이 논문은 중소벤처기업부 지역주력산업육성사업(R&D) (No.S2934141)과 과학기술정보통신부의 재원으로 한국연구재단의 지원을 받아 수행되었습니다(No. 2021R1A2C201471711).

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