• Title/Summary/Keyword: Muwang

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PCR-Based Detection of Densovirus Infection in Silkworm (Bombyx mori L.)

  • Hou Chengxiang;Li Muwang;Gui Zhongzheng;Xu Anying;Guo Xijie
    • International Journal of Industrial Entomology and Biomaterials
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    • v.11 no.2
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    • pp.135-138
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    • 2005
  • Two pairs of DNA primers were designed for the detection of the Zhenjiang (China) strain of Bombyx mori densonucleosis virus (BmDNV-Z). These primers were designed from the nucleotide sequence of major structural protein gene (putative VD1-ORF2). PCR amplification was attempted from different issues (including silk gland, blood, skin and midgut) and feces of the silkworm which infected wit BmDNV-Z were amplified by PCR. Both of the primers gave expected size of in the DNA bands from midgut and feces, but not in the DNA of silk gland, blood and skin. The two bands were sequenced, and their sequence were same as the sequence designed for. BmDNV-Z could be successfully detected in single silkworm after it was infected for 12 hrs, and could not be detected before 9 hrs after infected.

A Biovoltine Silkworm Variety, Huayuan${\times}$Dongshen, That is Resistant to Fluoride Contamination

  • Xu, Anying;Lin, Changqi;Hou, Chengxiang;Zhang, Yuehua;Li, Muwang;Sun, Pingjiang
    • International Journal of Industrial Entomology and Biomaterials
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    • v.13 no.1
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    • pp.1-5
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    • 2006
  • The major dominant fluoride-endurance (Dfe) gene was introduced into the commercial varieties by crossing and pedigree selection to breed silkworm races that could normally develop in the area that polluted by fluoride. After backcrossed for two generations, the Dfe gene was made homozygous, and individuals with good economic characters were selected to generate next generation. After 8 generations of selection, their characters became stable, and the silkworm variety which is resistant to fluoride, Huayuan${\times}$Dongsheng, for spring rearing were bred.

Breeding of Near Isogenic Lines of Silkworm (Bombyx mori L.)

  • Li, Muwang;Xu, Anying;Hou, Chengxiang;Zhang, Yuehua;Huang, Junting;Guo, Xijie
    • International Journal of Industrial Entomology and Biomaterials
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    • v.6 no.2
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    • pp.207-210
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    • 2003
  • Four different backcrossing methods were designed and 23 near isogenic lines (NILs) of 22 linkage groups were obtained using Hb as recurrent parent, the mutant gene lines which held markers as donor parents. Eleven of them had been mated with the recurrent parent for 10 times, and the others for 7∼8 times. The NILs of other 6 linkage groups are under way and had been backcrossed to the recurrent for 3∼4 times. These NILs will act important roles in the construction of molecular linkage map and gene location and positional cloning.

Screening of RAPD Markers for Fluoride Resistance in Bombyx mori L.

  • Chen, Keping;Yao, Qin;Li, Muwang;Wang, ong
    • International Journal of Industrial Entomology and Biomaterials
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    • v.7 no.1
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    • pp.11-14
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    • 2003
  • NF733xin, the near allele line was obtained by means of crossing and backcrossing the silkworm race T6, which contained fluoride resistance major gene, to race 733xin, which was highly susceptible to fluoride toxicity. Two hundred RAPD random primers were used in the RAPD analysis of these 3 strains. Two molecular markers, OPB-08850 and OPB-10917, were obtained. OPB-10917 was used to detect the backcross generations. It was found that all the fluoride resistant individuals in each backcross generation had the same special band. These results proved that this marker was reliable.

A Study on the Structure of the Foundations of Wooden Pagodas in Ancient Korea (한국 고대 목탑의 기단 및 심초부 축조기법에 관한 고찰 - 백제 사지를 중심으로 -)

  • Jung, Ja-young;Tahk, Kyung-baek
    • Korean Journal of Heritage: History & Science
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    • v.40
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    • pp.105-135
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    • 2007
  • Since now, the study of wooden pagodas in ancient Korea have been proceeded to the site plan, the devices and the goods of sarari containers and the reconstruction. They are based on the result of archaeological excavations and the historical material documents. But the study of the foundations of wooden pagodas have been unsatisfactory. In this paper, the investigations of the wooden pagodas in the Three Kingdoms are proceeded. Through this action, we have checked the method of the foundations, the type and installation of Simchoseok(central base stone) and the erection of Simju(central base pillar). In the result of this study, three types were in the method of the foundations-the construction of the upper on the surface of the earth, the construction of the upper and lower on the surface of the earth. And we know the types of the installation of Simchoseok were the underground-type central base stone and the ground-type central base stoned. The factors of the central base pillars were changed with the rising of central base stones from the lower on the surface of the earth to the surface of the foundation. This change was needed to construct the larger wooden pagodas than the ones of the past. In especially, the large wooden pagodas with the ground-type central base stoned were appeared in the king of Baekje, Muwang(A.D. 600~640). We assume that the method of the construction of larger wooden pagodas like that was forwarded to Silla and Japan.

Studies on the Hereditary Properties of SSR Marker in Silkworm (Bombyx mori L.)

  • Li Muwang;Li Minghui;Miao Xuexia;Lu Cheng;Huang Yongping
    • International Journal of Industrial Entomology and Biomaterials
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    • v.11 no.1
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    • pp.49-55
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    • 2005
  • Two BC1 group, c108 $\times$ (p50 $\times$ c108) and p50 $\times$ (p50 $\times$ c108), one group of F$_{2}$ progeny, (p50 $\times$ c108) F$_{2}$ ,and 3 SSR markers, F10539, FlO626 and FlO618 were used to test the hereditary properties of SSR markers in silkworm. FI0539, FlO626 were proved to be linkage, and FlO618 was proved to be independent to those two markers. According to Mendel's law, the recombinant value between F10539, FlO626 was calculated in all of these groups, and they were 8.55$\%$ (c108BC1), 8.02$\%$ (p50BC1) and 7.81 $\%$ (F$_{2}$) respectively. There was dominant difference among the crossing-over value using paired-samples tests by SPSS 10.0 software. This research proved that SSR markers were co-dominant in B. mori too, and F 2 progeny could be used to construct SSR linkage map although B. mori lacked of crossing over in females.

The Change of Management Methods on Iksan and the rearrangement of Sabi by Baekje (백제의 익산 경영 방식의 전환과 사비(泗沘) 재정비)

  • KIM, Nakjung
    • Korean Journal of Heritage: History & Science
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    • v.54 no.2
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    • pp.170-193
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    • 2021
  • In this article, I examine the transformation of the Iksan Royal Palace into a temple. And it was considered to mean a change in the way Iksan was managed by Baekje. The Iksan Royal Palace was converted into a temple between the establishment of Mireuksa Temple and Stone Pagoda. As a result, the transfer of the capital to Iksan was not achieved, and Iksan was transformed into a temple-centered city. In connection with this, the need to re-establishment the urban system in Sabi was increased, as it suffered a great flood in the Muwang era. As a result, the scope of the royal palace was expanded, the secondary palace was built, and the urban district was expanded throughout the capital city. It is presumed that King Mu gave a divine nature to Iksan and discriminated against Sabi. Accordingly, it is believed that the King Mu or King Uija stopped developing the city in Iksan and focused on the rearrangement of Sabi.

Formation of Efflorescence and Dissolving Experiments for Removing Control on the Stone Pagoda of the Mireuksaji Temple Site, Iksan, Korea (익산 미륵사지 석탑의 백화현상과 제어를 위한 용출실험)

  • Lee, Dong-Sik;Lee, Chan-Hee;Kim, Sa-Dug
    • Journal of Conservation Science
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    • v.19
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    • pp.5-18
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    • 2006
  • The Mireuksaji stone pagoda of the Iksan was built in Muwang King of Baekjae Kingdom and has maintained its present status through several rebuilding process. Recently, the structural unbalance of the pagoda has worsened, so for scientific maintenance recovery of its original pagoda form, the pagoda is under dismantling process. The original form of this pagoda is being presumed to be a plane square pagoda of nine stories, though only a portion of six stories remains until today. The destroyed part from collapse was liked in insecure pagoda form by Japanese in year of 1915. On the surface of the stone which has gone through the present time, efflorescence appeared as precipitation substance over crusted and due to this phenomenon, the structure and color of thee original stone haven't been preserved. Therefore, quantitative analysis on its kind, emitted status and contamination type of secondary inorganic contamination substance of the over crusted surface were analyzed. Also through reaction experiments, contamination type was defined and tests have been applied to find the requirements that will wash out the contaminants. The result of this study will provide the base of quantitative analysis on the kind of inorganic contamination of the surface of stone assets, and it shall also contribute to the application of scientific maintenance recovery of surface cleaner for efflorescence.

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A Study on the Awareness of Visitors to King Mu Trekking Course Program of Iksan (익산 무왕길 탐방프로그램 이용자의 인식에 대한 연구)

  • Park, Yool-Jin
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.36 no.4
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    • pp.80-93
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    • 2018
  • The following results could be obtained from the research on awareness and satisfaction of the tourists who joined the program 'A Journey in Search of King Mu's Road'. With regard to awareness of the site tour program, tourists replied that they participated in the program mainly because of their interest in education for their children. They expressed great needs for the municipal government office to maintain walkways, signboards and guide maps as well as to reinforce safety facilities as a measure to increase satisfaction with the site tour on the part of visitors and tourists. In terms of satisfaction with operation and management of the site tour program, the contents of the program were highly appreciated while it turned out necessary to rediscover garden culture resources and to develop and operate an expanded internal tour course for tourists to look around inside the relics by utilizing the recently excavated Baekje historical resources. As a consequence, it might be expected possible to experience and educate the local history and garden culture thanks to the historical culture and environmental values that have been preserved in the Baekje cultural circles. Moreover, the importance of walking trips will be recognized for its effects to allow those who get tired from daily life to come into contact with nature, practice self-reflection and care for their health as well.

The Abuse and Invention of Tradition from Maintenance Process of Historic Site No.135 Buyeo Gungnamji Pond (사적 제135호 부여 궁남지의 정비과정으로 살펴본 전통의 남용과 발명)

  • Jung, Woo-Jin
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.35 no.2
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    • pp.26-44
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    • 2017
  • Regarded as Korea's traditional pond, Gungnamj Pond was surmised to be "Gungnamji" due to its geological positioning in the south of Hwajisan (花枝山) and relics of the Gwanbuk-ri (官北里) suspected of being components to the historical records of Muwang (武王)'s pond of The Chronicles of the Three States [三國史記] and Sabi Palace, respectively, yet was subjected to a restoration following a designation to national historic site. This study is focused on the distortion of authenticity identified in the course of the "Gungnamji Pond" restoration and the invention of tradition, whose summarized conclusions are as follows. 1. Once called Maraebangjuk (마래방죽), or Macheonji (馬川池) Pond, Gungnamji Pond was existent in the form of a low-level swamp of vast area encompassing 30,000 pyeong during the Japanese colonial period. Hong, Sa-jun, who played a leading role in the restoration of "Gungnamji Pond," said that even during the 1940s, the remains of the island and stone facilities suspected of being the relics of Gungnamji Pond of the Baekje period were found, and that the traces of forming a royal palace and garden were discovered on top of them. Hong, Sa-jun also expressed an opinion of establishing a parallel between "Gungnamji Pond" and "Maraebangjuk" in connection with a 'tale of Seodong [薯童說話]' in the aftermath of the detached palace of Hwajisan, which ultimately operated as a theoretical ground for the restoration of Gungnamj Pond. Assessing through Hong, Sa-jun's sketch, the form and scale of Maraebangjuk were visible, of which the form was in close proximity to that photographed during the Japanese colonial period. 2. The minimized restoration of Gungnamji Pond faced deterrence for the land redevelopment project implemented in the 1960s, and the remainder of the land size is an attestment. The fundamental problem manifest in the restoration of Gungnamji Pond numerously attempted from 1964 through 1967 was the failure of basing the restorative work in the archaeological facts yet in the perspective of the latest generations, ultimately yielding a replication of Hyangwonji Pond of Gyeongbok Palace. More specifically, the methodologies employed in setting an island and a pavilion within a pond, or bridging an island with a land evidenced as to how Gungnamji Pond was modeled after Hyangwonji Pond of Gyeongbok Palace. Furthermore, Chihyanggyo (醉香橋) Bridge referenced in the designing of the bridge was hardly conceived as a form indigenous to the Joseon Dynasty, whose motivation and idea of the misguided restoration design at the time all the more devaluated Gungnamji Pond. Such an utterly pure replication of the design widely known as an ingredient for the traditional landscape was purposive towards the aesthetic symbolism and preference retained by Gyeongbok Palace, which was intended to entitle Gungnamji Pond to a physical status of the value in par with that of Gyeongbok Palace. 3. For its detachment to the authenticity as a historical site since its origin, Gungnamji Pond represented distortions of the landscape beauty and tradition even through the restorative process. The restorative process for such a historical monument, devoid of constructive use and certain of distortion, maintains extreme intimacy with the nationalistic cultural policy promoted by the Park, Jeong-hee regime through the 1960s and 1970s. In the context of the "manipulated discussions of tradition," the Park's cultural policy transformed the citizens' recollection into an idealized form of the past, further magnifying it at best. Consequently, many of the historical sites emerged as fancy and grand as they possibly could beyond their status quo across the nation, and "Gungnamji Pond" was a victim to this monopolistic government-led cultural policy incrementally sweeping away with new buildings and structures instituted regardless of their original space, and hence, their value.