International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Screening of RAPD Markers for Fluoride Resistance in Bombyx mori L.

  • Chen, Keping (Institute of Life Sciences, Jiangsu University) ;
  • Yao, Qin (Institute of Life Sciences, Jiangsu University) ;
  • Li, Muwang (Sericultural Research Institute, Chinese Academy of Agricultural Sciences) ;
  • Wang, ong (Institute of Life Sciences, Jiangsu University)
  • Published : 2003.09.01
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Abstract

NF733xin, the near allele line was obtained by means of crossing and backcrossing the silkworm race T6, which contained fluoride resistance major gene, to race 733xin, which was highly susceptible to fluoride toxicity. Two hundred RAPD random primers were used in the RAPD analysis of these 3 strains. Two molecular markers, OPB-08850 and OPB-10917, were obtained. OPB-10917 was used to detect the backcross generations. It was found that all the fluoride resistant individuals in each backcross generation had the same special band. These results proved that this marker was reliable.

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References

  1. Demeke, T., A. Lorache and D. A. Gandet (1996) A DNA marker for the Bt-lO common bunt resistance gene in wheat Genome 39, 51-55
  2. Hartl, L., H. Weiss, U. Stephan, F. J. Zeller and A. Jahoor (1995) Molecular indentification of powdery mildew resistance genes in common wheat (Triticum aestivum L.). Theor. Appl. Genet. 90, 601-606
  3. Lin, C. Q., Q. Yao, D. X. Wu, K. P. Chen and Q. Q. Fang (1996) An investigation and correlation analysis on silkworm germplasms endurance to fluoride. Acta Sericologica Sinica 22, 253-255
  4. Lin, C. Q., Y. D. Mi, Q. Yao, D. X. Wu and Z. J. Wei (1997) Discovery of the major dominant gene of fluoride resistance in Bombyx mori L. Acta Sericotogica Sinica 23, 237-239
  5. Williams, J. G. K., A. R. Kubelik, K. J. Livak, J. A. Rafalski and S. V. Tingey (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18, 6531-6535 https://doi.org/10.1093/nar/18.22.6531
  6. Zhao, Y., H. Y. Qian, K. P. Chen and S. M. He (1996) Sudies on resistance of current silkworm races Bombyx mori to fluoride and nuclear polyhedrosis virus. Acta Sericologica Sinica 22, 219-223