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Screening of RAPD Markers for Fluoride Resistance in Bombyx mori L.  

Chen, Keping (Institute of Life Sciences, Jiangsu University)
Yao, Qin (Institute of Life Sciences, Jiangsu University)
Li, Muwang (Sericultural Research Institute, Chinese Academy of Agricultural Sciences)
Wang, ong (Institute of Life Sciences, Jiangsu University)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.7, no.1, 2003 , pp. 11-14 More about this Journal
Abstract
NF733xin, the near allele line was obtained by means of crossing and backcrossing the silkworm race T6, which contained fluoride resistance major gene, to race 733xin, which was highly susceptible to fluoride toxicity. Two hundred RAPD random primers were used in the RAPD analysis of these 3 strains. Two molecular markers, OPB-08850 and OPB-10917, were obtained. OPB-10917 was used to detect the backcross generations. It was found that all the fluoride resistant individuals in each backcross generation had the same special band. These results proved that this marker was reliable.
Keywords
Bombyx mori; Near allele line; Fluoride resistance; RAPD marker;
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