• Biomolecules & Therapeutics
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• 제9권1호
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• pp.46-50
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• 2001

The current study was performed to determine the acute oral toxicity of a crude extract of sanghwang mushroom (Phellinus linteus), in SD rats. 5 rats of each sex were orally treated with a single dose of extract of sanghwang mushroom at doses of 0, 500, 1,000, 2,000 mg/kg, respectively. After the treatment, clinical signs and body weight change, the food and water consumption were observed for 14 days. All animals survived during the study and did not show any clinical signs. Body weight gain showed no significant difference between the control and treated rats. However, body weight gain delayed in high dose group (2,000 mg/kg) on day 1~3 after administration. Another 5 rats of each sex were orally treated with a single dose of extract of sanghwang mushroom at dosages 4,000, 5,000 mg/kg respectively, but all animals survived during the study and did not show any clinical signs. It is suggested that LD$_{50}$ of extract of sanghwang mushroom by oral administration was estimated to be over 5,000 mg/kg in both sexes of rats.s.

• Preventive Nutrition and Food Science
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• 제6권1호
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• pp.16-18
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• 2001

The inhibitory effect of onion extract on mushroon tyrosinase activity was investigated. The enzyme activity was inhibited by 96.3% with th addition of onion extract. The inhibitor action of onion extract toward mushroom tyrosinase activity slightly increased after heat treatment at 10$0^{\circ}C$ for 10 mn (97.5%) and decreased after incubation of the extract at pH 2.3 for 3hrs (79.9%). However, the inhibitor action of the extract after dialysis decreased to18.8%. The onion extract showed drastic inhibition of the browning in mushroom.

• 한국버섯학회지
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• 제12권1호
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• pp.24-28
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• 2014

표고버섯균의 균사생육에 적합한 배지로는 PDA 배지, MYA 배지로 조사되었으며 배양환경 조사로 균사생육에 적합한 온도는 $25^{\circ}C$를 나타났다. 영양원 선발 결과 탄소원은 2%의 농도에서 Malt extract를 사용하였을 때 균사생장 및 밀도가 높게 나타났다. 질소원은 0.2%의 농도에서 Yeast extract를 사용하였을 때 균사생장 및 밀도가 높게 나타났다. 표고버섯(산조701호)의 수종별 추출액 배지를 이용한 결과, 신갈나무, 굴참나무, 상수리나무, 밤나무에서는 PDA(대조구)와 같이 생장이 우수하였다. 배양기간에 따른 버섯 생산성을 조사 해본 결과, 배양 110일에서 120일에서 우수한 수확량을 보였다. 재배수종에 따른 생산성은 굴참나무에서 가장 좋은 결과를 보였다.

• 동의생리병리학회지
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• 제27권2호
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• pp.239-245
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• 2013

In in vitro study, the anti-platelet aggregation effect of Shiitake mushroom extract was examined by measuring the collagen induced platelet aggregation and the DPPH radical scavenging. In in vitro study, anti-thrombotic effect of Shiitake mushroom extract was examined using the carotid artery thrombosis rat model. Carotid artery thrombosis rat model was made by 35% $FeCl_3$ treatment. After that, we investigate thrombus weight and blood flow. In platelet aggregation test, the extract significantly inhibited platelet aggregation in a concentration dependent manner(p<.001). Also, extract increased DPPH radical scavenging activity in a concentration dependent manner. Extract significantly inhibited thrombus weight to compare with control group. And blood passage time were shorter in the Shiitake mushroom extract supplemented groups than in control group. These results provide experimental evidence that Shiitake mushroom can be used to prevent platelet aggregation and thrombosis, then could apply the clinical diseases such as cardiovascular disease, and so on.

• 동의생리병리학회지
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• 제27권4호
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• pp.409-415
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• 2013

We attempted to determine the action target of Shiitake mushroom extract with a known anti-hyperlipidemic effect in poloxamer(P) 407-induced hyperlipidemia model. We investigated the anti-hyperlipidemic effects of the water extract from Shiitake mushroom on the progress of high fat diet for 4 weeks. Experimental rats were divided into 5 different experimental groups including an normal group (normal diet; n=10), control group (hyperlipidemia; n=10), Experimental group I (hyperlipidemic rats treated with Shiitake mushroom extract (100 mg/kg, PO), n=10), Experimental group II (hyperlipidemic rats treated with Shiitake mushroom extract (300 mg/kg, PO), n=10), and Experimental group III (hyperlipidemic rats treated with Shiitake mushroom extract (500 mg/kg, PO), n=10). It is to analysis changes in body weight, visceral fat weight, blood lipid profiles, HMG-CoA reductase and histological findings. Body weight and epididymal fat weight was not significantly change in experimental groups (p>0.05). The level of total cholesterol, TG, arthrogenic index, and HMG-CoA reductase were significantly lower in experimental groups than control group (p<0.05). These results suggested that the Shiitake mushroom extract administration may act by inhibitory the release of cholesterol related factors and HMG-CoA from the hepatocyte without liver and kidney cell damage in hyperlipidemia rats.

• 생약학회지
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• 제35권1호통권136호
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• pp.92-97
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• 2004

Effect of AGI $({\alpha}-Glucosidase\;Inhibitor)-1120$, pine (Pinus densiflora) bark extract and Chaga mushroom (Inonotus obliquus) - and Chaga mushroom mycelium extracts on cellular $NF-{\kappa}B$ activation in malignant human keratinocytes (SCC-13) were evaluated to elucidate the possible correlation of $NF-{\kappa}B$ with antioxidant activity. The antioxidant activities of these natural products were examined in three different evaluation methods, i.e., lipid peroxidation value (POV) evaluation test, and 1,1diphenyl-2-picrylhydrazyl radical (DPPH) and nitric oxide (NO) scavenging test. In a cell-based $NF-{\kappa}B$ monitoring assay systern, all samples revealed the downregulatory profiles on the cellular $NF-{\kappa}B$ activity. AGI -1120 (1, 2 mg) and Chaga mushroom extract (0.05, 0.1 mg) downregulated the $NF-{\kappa}B$ activity in a dose-dependent manner. Chaga mushroom mycelium extract (5 mg) significantly inhibited the $NF-{\kappa}B$ activity (p<0.05). Although AGI-1120 and Chaga mushroom mycelium extract exhibited no antioxidant activities evaluated in pay, Chaga mushroom extract showed antioxidant in a dose-dependent manner at concentrations of $0.05{\sim}1$ mg. While AGI-1120 and Chaga mushroom extract possessed a relatively potential DPPH radical scavenging activity, the NO scavenging activity of Chaga mushroom extract $(SC_{50}:47\;{mu}g)$ was higher than the known antioxidant, vitamin C $(SC_{50}:77\;{mu}g)$. These results suggest that AGI-1120 and Chaga mushroom- and Chaga mushroom mycelium extracts may serve as an useful radical scavenging antioxidant agents with $NF-{\kappa}B$ inhibitory effect in human skin.

• Advances in Traditional Medicine
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• 제4권1호
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• pp.22-27
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• 2004

Cisplatin is a highly effective and extensively used anticancer drug. Higher doses of cisplatin manifest acute nephrotoxicity and this is one of the limiting factors of this drug in cancer chemotherapy. The effect of the oyster mushroom extract to ameliorate cisplatin ( cis platinum (II) diammine dichloride) induced nephrotoxicity and restoration of antioxidant defence system in mice was investigated. The investigations showed that prior administration of methanolic extract of Pleurotus florida at a dose of 500 and 1000mg/Kg body weight significantly reduced elevated serum creatinine and urea levels and increased superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities in the kidney, consequent to cisplatin treatment, in a dose dependent manner. The extract restored the decreased reduced glutathione (GSH) activity and increased malondialdehyde (MDA) level due to cisplatin administration. The results thus indicated that oyster mushroom extract rendered significant protection against cisplatin induced nephrotoxicity and depletion of antioxidant defence system in a dose dependent manner. Since oyster mushrooms are excellently edible and non-toxic, the finding reported here is of significant use in cancer chemotherapy.

• 동의생리병리학회지
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• 제21권1호
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• pp.204-208
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• 2007

Chaga mushroom extract is well known as immune modulator and anti-cancer agent. However, the molecular mechanism by which Chaga exerts cell cycle arrest and apoptosis of cancer cells is poorly understood. In this study, we demonstrated anti-proliferative effects of Chaga extract on murine melanoma B16 cells. Chaga extract dose-dependently inhibited cell growth along with the arrest of G0/G1 phase and the induction of apoptotic cell death. Treatment with Chaga extract resulted in a decrease of cyclin E, cyclin D1, cdk 2, cdk 4 expression levels. Furthermore, in vivo inoculation study of B16 melanoma cells into Balb/c mice Chaga extract markedly suppressed the metastatic growth of tumor cells (6 folds, p<0.05,). These results indicate that Chaga mushroom extract induces apoptosis of B16 melanoma cells through arrest of G0/G1 phase in cell cycle.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.106-106
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• 2019

Human needs energy to maintain metabolisms, and these energy sources were uptake foods or nutritions. The most effective source was known for glucose among the nutrients, furthermore the glucose is an important source of energy for blood cells and control brain maintenences cells. But as food is plentiful and eating habits become more westernized, fast food and irregular meal times by works. Nowadays, diabetes were rapidly increased by malnutriton and obesity. Diabetes was the sixth highest on the list of causes of death in Korea, released by the Statistics Korea in 2015, which is considered a serious social problem for adult diseases. Therefore, this study aims to establish the optimal hot water extraction conditions of mixed herbs extract mixture compounds that are effective in diabetes. The independent factors were extraction temperature (X1: $40-120^{\circ}C$), extraction time (X2: 2-10 hrs.), and the ratio of water to sample (X3: 40-200 mg/mL). Their effects were assessed on dependent variables of the extract properties, which included soluble solid contents, Brix of sample extract, total polyphenols content, total flavonoids content and DPPH Radical scavenging activity. As a result, the content of total polyphenol content was the highest in No.12(6 hrs, $120^{\circ}C$, 67 mg/mL) and the highest total flavonoid contents was found in No.16(6 hrs, $80^{\circ}C$, 40 mg/mL). DPPH Radical scavenging activity showed the highest activity in No.7(8 hrs, $100^{\circ}C$, 100 mg/mL).

• 생명과학회지
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• 제14권3호
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• pp.514-520
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• 2004

유산균 발효유, 버섯(표고, 영지, 느타리) 추출물 및 이를 함유한 유산균 발효유가 SD계 암컷 흰쥐의 각 조직 과산화지질 농도 및 DPPH 전자공여능에 미치는 항산화 효과를 검토하였다. 표고, 영지, 느타리버섯 추출물의 Folin-Denis 법에 의한 총 폴리페놀 화합물 농도는 각각 0.34, 0.20, 0.34%로 나타났다. 유산균 발효유, 버섯 추출물, 버섯 추출물 함유 유산균 발효유 및 이들 시료의 standard로서 사용된 BHT의 DPPH법에 의한 전자공여능은 각각 33.9, 34.9, 51.9 및 95.6%로 나타났다. 실험 식이군은 정상군, 콜레스테롤군, 콜레스테롤 + 유산균 발효유군, 콜레스테롤 + 버섯 추출물군 및 콜레스테롤+버섯 추출물 함유 유산균 발효 유군으로 구성하였다. 간 조직 및 microsome 획분과 과산화지질 농도는 콜레스테롤+유산균 발효유군 및 콜레스레롤+버섯 추출물 함유 유산균 발효유군이 다른 실험군 보다 유의적으로 감소하였다. 신장 조직의 과산화지질 농도는 정상군에 비해 콜레스테롤군에서 현저히 증가하였고, 이러한 콜레스테롤 식이에 의한 증가는 유산균 발효유 및 버섯 추출물 함유 유산균 발효유 첨가로 인해 유의적으로 감소하였다. 한편, 심장 및 비장 조직, 이들의 microsome 및 mitochondria 획분의 과산화지질 농도는 각 실험군간에 유의한 차이가 없었다. 본 실험의 결과, 버섯 추출물 함유 유산균 발효유 식이는 흰쥐 간장 및 신장 조직의 과산화지질 생성을 감소시키는 작용이 있는 것으로 밝혀졌다.