Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of Shiitake Mushroom on Anti-platelet Aggregation and Anti-thrombotic

표고버섯 추출물의 항혈소판 응집 및 항혈전 효과

  • Received : 2013.02.01
  • Accepted : 2013.03.27
  • Published : 2013.04.25
Download PDF
⟨ Previous Next ⟩

Abstract

In in vitro study, the anti-platelet aggregation effect of Shiitake mushroom extract was examined by measuring the collagen induced platelet aggregation and the DPPH radical scavenging. In in vitro study, anti-thrombotic effect of Shiitake mushroom extract was examined using the carotid artery thrombosis rat model. Carotid artery thrombosis rat model was made by 35% $FeCl_3$ treatment. After that, we investigate thrombus weight and blood flow. In platelet aggregation test, the extract significantly inhibited platelet aggregation in a concentration dependent manner(p<.001). Also, extract increased DPPH radical scavenging activity in a concentration dependent manner. Extract significantly inhibited thrombus weight to compare with control group. And blood passage time were shorter in the Shiitake mushroom extract supplemented groups than in control group. These results provide experimental evidence that Shiitake mushroom can be used to prevent platelet aggregation and thrombosis, then could apply the clinical diseases such as cardiovascular disease, and so on.

Keywords

References

  1. Lefevre, M., Kris-Etherton, P.M., Zhao, G., Tracy, R.P. Dietary fatty acids, hemostasis, and cardiovascular disease risk. J Am Diet Assoc. 104(3):410-419, 2004. https://doi.org/10.1016/j.jada.2003.12.022
  2. Mustard, J.F., Packham, M.A. Factors influencing platelet function: adhesion, release, and aggregation. Pharmacol Rev. 22(2):97-187, 1970.
  3. Miwa, K., Kambara, H., Kawai, C. Effect of aspirin in large doses on attacks of variant angina. Am Heart J. 105(2):351-355, 1983. https://doi.org/10.1016/0002-8703(83)90548-3
  4. Korean national statistical office. Available at http://kosis.kr/gen_etl/start.jsp?orgId=101&tblId=DT_1B34 E13&conn_path=I2&path. Assessed September 13, 2012.
  5. 이현진. 양파즙의 섭취가 경계고지혈증 대상자의 혈중지질에 미치는 영향연구, 창원대학교 교육대학원, 2009.
  6. 정창주. 유근피 추출물이 흰쥐의 황산화계 및 지질대사에 미치는 영향, 조선대학교 대학원, 2006.
  7. Lim, S.S., Lee, J.H. Effect atremisa princeps var orientails and ccircium japonicum varussuiense on serum lipid of hyperlipidemic rat. Korean J Nutr. 30: 12-18, 1997.
  8. Longenecker, G.L., Swift, I.A., Bowen, R.J., Beyers, B.J., Shah, A.K. Kinetics of ibuprofen effect on platelet and endothelial prostanoid release. Clin Pharmacol Ther. 37: 343-348, 1985. https://doi.org/10.1038/clpt.1985.50
  9. Yen, G.C., Hsieh, C.L. Antioxidant activity of extracts from Du-Zhong (eucommia ulmoides) toward various lipid peroxidation models in vitro. J Agric Food Chem. 46: 3431-3436, 1998. https://doi.org/10.1021/jf9707041
  10. Bano, Z., Rajarathnam, S. Pleurotus mushrooms. Part II. Chemical composition, nutritional value, post-harvest physiology, preservation, and role as human food. Crit Rev Food Sci Nutr. 27(2):87-158, 1988. https://doi.org/10.1080/10408398809527480
  11. Sung, J.M., Yoo, Y.B., Cha, D.R. Mushroom science. Kyohaksa, Seoul. Korea, 1998.
  12. Choi, M.Y., Jung, T.Y., Hahm, K.J. Cytotoxic effects of hot water soluble polysaccharides from mushroom, lentinus edodes and vitamin A & E Supplementation against P388 cells. Korean J Nutrition. 28(11):1091-1099, 1995.
  13. Blois, M.S. Antioxidant determination by the use of stable free radical. Nature. 26: 1199-1200, 1958.
  14. Wang, X., Cheng, Q., Xu, L., Feuerstein, G.Z., Hsu, M.Y., Smith, P.L., Seiffert, D.A., Schumacher, W.A., Ogletree, M.L., Gailani, D. Effect of factor Ⅸ or factor Ⅺ deficiency on ferric chloride-induced carotid artery occlusion in mice. J Thromb Haemost. 3: 695-702, 2005. https://doi.org/10.1111/j.1538-7836.2005.01236.x
  15. Yang, S.A., Im, N.K., Lee, I.S. Effects of methanolic extract from salvia miltiorrhiza bunge on in vitro antithrombotic and antioxidative activities. Korean J Food Sci Technol. 39(1):83-87, 2007.
  16. 양원경, 성윤영, 김호경. 호장근 추출물의 항혈전 효능 및 혈소판 응집 억제작용. J Korean Soc Food Sci Nutr. 41(2):168-173, 2012. https://doi.org/10.3746/jkfn.2012.41.2.168
  17. Fan, J., Zhang, Y., Chang, X., Zhang, B., Jiang, D., Saito, M., Li, Z. Antithrombotic and fibrinolytic activities of methanolic extract of aged sorghum vinegar. J Agric Food Chem. 57(18):8683-8687, 2009. https://doi.org/10.1021/jf901680y
  18. 전국한의과대학 병리학교실, 한방병리학, 일중사, pp 81-85, 2002.
  19. 張機. 仲景全書. 도서출판 정담, 25, 1999.
  20. 박종형외 9명. 補陽還五湯이 血栓生成關與因子에 미치는 影響. 대한한방내과학회지, 21(5):829-837, 2000.
  21. Liu, S., Jiang, S., Wu, Z., Lv, L., Zhang, J., Zhu, Z., Wu, S. Identification of inhibitors of the HIV-1 gp41 six-helix bundle formation from extracts of chinese medicinal herbs prunella vulgaris and rhizoma cibotte. Life Sci. 71(15):1779-1791, 2002. https://doi.org/10.1016/S0024-3205(02)01939-2
  22. Burggraf, D., Vosko, M.R., Schubert, M., Stassen, J.M., Hamann, G.F. Different therapy options protecting microvasculature after experimental cerebral ischaemia and reperfusion. Thromb Haemost. 103(5):891-900, 2010. https://doi.org/10.1160/TH09-07-0500
  23. Swenson, S., Markland, F.S. Jr. Snake venom fibrin(ogen)olytic enzymes. Toxicon. 45(8):1021-1039, 2005. https://doi.org/10.1016/j.toxicon.2005.02.027
  24. Mine, Y., Wong, A.H.K., Jiang, B. Fibrinolytic enzymes in Asian traditional fermented foods. Food Res Int. 38(3):243-250, 2005. https://doi.org/10.1016/j.foodres.2004.04.008
  25. Park, M.H., Oh, K.Y., Lee, B.W. Anti-cancer activity of lentinus edodes and pleurotus ostreatus. Korean J Food Sci Technol. 30(3):702-708, 1998.
  26. Lee, B.W., Park, K.M. Anti-tumor activity of protein-bound polysaccharides extracted from mycelia of lentinus edodes. Korean J Food Sci Technol. 30(3):665-671, 1998.
  27. Park, K.M., Lee, B.W. Extraction and purification of antitumor protein-bound polysaccharides from mycelia of lentinus edodes. Korean J Food Sci Technol. 30(5):1236-1242, 1998.
  28. Ma, S.J. Effects of the substances extracted from dried mushroom (Lentinus edodes) by several organic solvents on the stability of fat. Korean J Food Sci Technol. 15(2):150-154, 1983.
  29. Cho, Y.J., Kim, H.A., Bang, M.A., Kim, E.H. Effect of dietary mushroom on blood glucose levels, lipid concentrations and glutathione enzymes in streptozotocininduced diabetic rats. Korean J Nutri. 35(2):183-191, 2002.
  30. Kim, Y.S., Lim, C.H. and Cho, N.S. Hemagglutinative activity of lectin Isolated from shiitake, lentinula edodes. Korean J Mycol. 30(1):31-36, 2002. https://doi.org/10.4489/KJM.2002.30.1.031
  31. Chihara, G., Maeda, Y., Hamuro, J., Sasaki, T., Fukuoka, F. Inhibition of mouse sarcoma 180 by polysaccharides from Lentinus edodes(Berk.) sing. Nature. 222(5194):687-688, 1969. https://doi.org/10.1038/222687a0
  32. Ito, Y., Toyoda, N., Suzuki, M., Iwaida. Gas-liquid chromatographic determination. J of Food Sci. 43(4):1287, 1978. https://doi.org/10.1111/j.1365-2621.1978.tb15289.x
  33. Morita, K., Kobayashi, S. Isolation, structure, and synthesis of lenthionine and its analogs. Chem. Pharm. Bull. 15(7): 988-993, 1967. https://doi.org/10.1248/cpb.15.988
  34. Michelson, A.D., Frelinger, A.L. 3rd., Furman, M.I. Current options in platelet function testing. Am J Cardiol. 98(10A):4N-10N, 2006.
  35. Breddin, H.K. Can platelet aggregometry be standardized?. Platelets. 16(3-4):151-158, 2005. https://doi.org/10.1080/09537100400020161
  36. Vranova, E., Inze, D., Van Breusegem, F. Signal transduction during oxidative stress. J Exp Bot. 53(372):1227-1236, 2002. https://doi.org/10.1093/jexbot/53.372.1227
  37. Sies, H. Oxidative stress: from basic research to clinical application. Am J Med. 91(3C):31S-38S, 1991.
  38. Trush, M.A., Mimnaugh, E.G., Gram, T.E. Activation of pharmacologic agents to radical intermediates. Implications for the role of free radicals in drug action and toxicity. Biochem Pharmacol. 31(21):3335-3346, 1982. https://doi.org/10.1016/0006-2952(82)90609-8
  39. Kurz, K.D., Main, B.W., Sandusky, G.E. Rat model of arterial thrombosis induced by ferric chloride. Thromb Res. 60: 269-280, 1990. https://doi.org/10.1016/0049-3848(90)90106-M
  40. 허은정, 이인선, 강형원, 전원경. 경동맥 손상 및 혈전을 유발한 동물 모델에서 續命湯의 효능 검증. Kor J Oriental Physiology & Pathology. 26(5):732-737, 2012.
  41. Kang, J.A., Kang, J.S. Effect of garlic an onion on plasma an liver cholesterol and triglyceride and plate aggregation in rat basal or cholesterol supplemented diets. Korean J Nutr. 32: 132-138, 1997.
  42. Shin, K.S., Lee, J.J., Jin, Y.R., Yu, J.Y., Park, E.S., Im, J.H., You, S.H., Oh, K.W., Lee, M.K., Wee, J.J., Kim, Y.S., Yun, Y.P. Effect of korean red ginseng extract on blood circulation in healthy volunteers: A randomized, double-blind, placebo-controlled trial. J Ginseng Res. 31(2):109-116, 2007. https://doi.org/10.5142/JGR.2007.31.2.109