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Cha-ga Mushroom Water Extract induces G0/G1 Arrest in B16-F10 Melanoma cells  

Youn, Myung-Ja (Vestibulocochlear Research Center & Department of Microbiology, Wonkwang University School of Medicine)
Song, Jeong-Hoon (Plastic & Reconstructive Surgery, Wonkwang University School of Medicine)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.21, no.1, 2007 , pp. 204-208 More about this Journal
Abstract
Chaga mushroom extract is well known as immune modulator and anti-cancer agent. However, the molecular mechanism by which Chaga exerts cell cycle arrest and apoptosis of cancer cells is poorly understood. In this study, we demonstrated anti-proliferative effects of Chaga extract on murine melanoma B16 cells. Chaga extract dose-dependently inhibited cell growth along with the arrest of G0/G1 phase and the induction of apoptotic cell death. Treatment with Chaga extract resulted in a decrease of cyclin E, cyclin D1, cdk 2, cdk 4 expression levels. Furthermore, in vivo inoculation study of B16 melanoma cells into Balb/c mice Chaga extract markedly suppressed the metastatic growth of tumor cells (6 folds, p<0.05,). These results indicate that Chaga mushroom extract induces apoptosis of B16 melanoma cells through arrest of G0/G1 phase in cell cycle.
Keywords
Chaga mushroom; apoptosis; cell cycle arrest;
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