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Downregulatory Effect of AGI-1120 $({\alpha}-Glucosidase Inhibitor)$ and Chaga Mushroom (Inonotus obliquus) on Cellular $NF-{\kappa}B$ Activation and Their Antioxidant Activity  

Song, Hee-Sun (Department of Clinical Pathology and Food & Nutrition, and Bioindustry & Technology Research Institute, Kwangju Health College)
Lee, Young-Jong (Department of Clinical Pathology and Food & Nutrition, and Bioindustry & Technology Research Institute, Kwangju Health College)
Kim, Seung-Kyoon (Department of Clinical Pathology and Food & Nutrition, and Bioindustry & Technology Research Institute, Kwangju Health College)
Moon, Won-Kuk (En-Biotechnology Co., Ltd)
Kim, Dong-Woo (En-Biotechnology Co., Ltd)
Kim, Yeong-Shik (Natural Products Research Institute, College of Pharmacy, Seoul National University)
Moon, Ki-Young (Department of Clinical Pathology and Food & Nutrition, and Bioindustry & Technology Research Institute, Kwangju Health College)
Publication Information
Korean Journal of Pharmacognosy / v.35, no.1, 2004 , pp. 92-97 More about this Journal
Abstract
Effect of AGI $({\alpha}-Glucosidase\;Inhibitor)-1120$, pine (Pinus densiflora) bark extract and Chaga mushroom (Inonotus obliquus) - and Chaga mushroom mycelium extracts on cellular $NF-{\kappa}B$ activation in malignant human keratinocytes (SCC-13) were evaluated to elucidate the possible correlation of $NF-{\kappa}B$ with antioxidant activity. The antioxidant activities of these natural products were examined in three different evaluation methods, i.e., lipid peroxidation value (POV) evaluation test, and 1,1diphenyl-2-picrylhydrazyl radical (DPPH) and nitric oxide (NO) scavenging test. In a cell-based $NF-{\kappa}B$ monitoring assay systern, all samples revealed the downregulatory profiles on the cellular $NF-{\kappa}B$ activity. AGI -1120 (1, 2 mg) and Chaga mushroom extract (0.05, 0.1 mg) downregulated the $NF-{\kappa}B$ activity in a dose-dependent manner. Chaga mushroom mycelium extract (5 mg) significantly inhibited the $NF-{\kappa}B$ activity (p<0.05). Although AGI-1120 and Chaga mushroom mycelium extract exhibited no antioxidant activities evaluated in pay, Chaga mushroom extract showed antioxidant in a dose-dependent manner at concentrations of $0.05{\sim}1$ mg. While AGI-1120 and Chaga mushroom extract possessed a relatively potential DPPH radical scavenging activity, the NO scavenging activity of Chaga mushroom extract $(SC_{50}:47\;{mu}g)$ was higher than the known antioxidant, vitamin C $(SC_{50}:77\;{mu}g)$. These results suggest that AGI-1120 and Chaga mushroom- and Chaga mushroom mycelium extracts may serve as an useful radical scavenging antioxidant agents with $NF-{\kappa}B$ inhibitory effect in human skin.
Keywords
AGI-1120 $({\alpha}-Glucosidase inhibitor)-1120$; Chaga Mushroom (Inonotus obliquus); $NF-{\kappa}B$ activity; Antioxidant; Radical scavenging activity;
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