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Isolation, Regeneration and the Maturation Induction of Free-Living Gametophytes of Undaria pinnatifida (Phaeophyceae) (갈조류 미역 유리배우체의 분리, 재생 및 성숙 유도)

  • Yoo, Hyun Il;Kim, Su Hong;Lee, Gi hyun;Ha, Dong Soo;Hwang, Eun Kyoung
    • Korean Journal of Environmental Biology
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    • v.35 no.4
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    • pp.622-630
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    • 2017
  • The induction of regeneration and the maturation of the free-living gametophytes of Undaria pinnatifida were studied at temperatures(5, 10, 15 and $20^{\circ}C$), irradiance (5, 10, 20 and $40{\mu}mol\;photons\;m^{-2}\;s^{-1}$) and photoperiods (14 : 10, 12 : 12 and 10 : 14 h L: D). Female gametophyte fragments were maintained in active regeneration without them reaching sexual maturity under conditions of $15^{\circ}C$, $20{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 10 : 14 h (L : D); by contrast, the conditions for male gametophytes were slightly different at $15^{\circ}C$, $5{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 14 : 10 h (L: D). The sexual maturation of female and male gametophytes took place under $5^{\circ}C$, $20-40{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 10 : 14 h (L : D) and $15^{\circ}C$, $40{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 10 : 14 h (L : D), respectively. These results provide basic information for controlling the regeneration and maturation of free-living gametophytes for srain improvement as well as the cross breeding of U. pinnatifida.

Syrosingopine and Reserpine on Pressor Action of Norepinephrine in Rabbits (Syrosingopine 및 Reserpine의 Norepinephrine에 대한 가토혈압반응(家兎血壓反應)에 미치는 영향(影響))

  • Shim, Chang-Sub
    • The Korean Journal of Pharmacology
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    • v.6 no.1
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    • pp.27-35
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    • 1970
  • 1) In whole anesthetized rabbits and spinal rabbits, the potentiating effect of syrosingopine and reserpine on pressor action of norepinephrine (NE) was compared. 2) The doses of syrosingopine and reserpine were 8, 40, $200\;{\mu}g$ and 1 mg per kg of body weight. The pressor responses to NE(0.1, 0.5, 0.25, 1.2, 6.0, 30.0, $150.0\;{\mu}g/kg$) were examine at 4, 10 and 24 hours after administration of the drugs. 3) In whole rabbits, potentiation by syrosingopine of pressor effect of NE was observed after administration of above the dose of $40\;{\mu}g/kg$, potentiation by reserpine was above $8\;{\mu}g/kg$. The maximal potentiation was achieved 10 hours after administration of $200\;{\mu}g/kg$ of each agent. 4) In spinal rabbits, syrosingopine $(200\;{\mu}g/kg)$ produced slight potentiation of pressor effect of NE. The same dose of reserpine produced more pronounced potentiation. 5) In the whole rabbits carbachol inhibited the potentiation observed 4 hours after administration of $40\;{\mu}g/kg$ of reserpine and syrosingopine. 6) In spinal rabbits, the potentiation observed 10 hours after $200\;{\mu}g/kg$ of reserpine and syrosingopine was inhibited by administration of carbachol. 7) The onset of potentiation of the pressor effect of NE was within 15 min after administration of syrosingopine and reserpine (1 mg/kg, each). 8) The above data suggest that the development of NE supersensitivity by syrosingopine and reserpine in rabbits has more intimate relationship with the decrease of central catecholamine contents than with that of peripheral ones. The depression of central sympathetic tone produced by these agents seems to play an important role in development of supersensitivity.

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SOME RESULTS RELATED WITH POISSON-SZEGÖKERNEL AND BEREZIN TRANSFORM

  • Yang, Gye Tak;Choi, Ki Seong
    • Journal of the Chungcheong Mathematical Society
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    • v.24 no.3
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    • pp.417-426
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    • 2011
  • Let ${\mu}$ be a finite positive Borel measure on the unit ball $B{\subset}{\mathbb{C}}^n$ and ${\nu}$ be the Euclidean volume measure such that ${\nu}(B)=1$. For the unit sphere $S=\{z:{\mid}z{\mid}=1\}$, ${\sigma}$ is the rotation-invariant measure on S such that ${\sigma}(S) =1$. Let ${\mathcal{P}}[f]$ be the Poisson-$Szeg{\ddot{o}}$ integral of f and $\tilde{\mu}$ be the Berezin transform of ${\mu}$. In this paper, we show that if there is a constant M > 0 such that ${\int_B}{\mid}{\mathcal{P}}[f](z){\mid}^pd{\mu}(z){\leq}M{\int_B}{\mid}{\mathcal{P}}[f](z){\mid}^pd{\nu}(z)$ for all $f{\in}L^p(\sigma)$, then ${\parallel}{\tilde{\mu}}{\parallel}_{\infty}{\equiv}{\sup}_{z{\in}B}{\mid}{\tilde{\mu}}(z){\mid}<{\infty}$, and we show that if ${\parallel}{\tilde{\mu}{\parallel}_{\infty}<{\infty}$, then ${\int_B}{\mid}{\mathcal{P}}[f](z){\mid}^pd{\mu}(z){\leq}C{\mid}{\mid}{\tilde{\mu}}{\mid}{\mid}_{\infty}{\int_S}{\mid}f(\zeta){\mid}^pd{\sigma}(\zeta)$ for some constant C.

Temperature-Dependence of Poly-Si Thin film Transistors (다결정 실리콘 박막 트랜지스터의 온도 의존성)

  • 이정석;이용재
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 1999.05a
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    • pp.403-406
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    • 1999
  • The influence of temperature variation (25~125$^{\circ}C$) on poly-Si thin-film transistors (TFT's) was investigated by examining the electrical properties change of poly-Si films formed by solid phase crystallization (SPC). The n-channel poly-Si TFT's fabricated by SPC with channel length of 1.5 and loon ,respectively, exhibit good characteristics with a high ${\mu}$$\sub$FE/ ($\geq$82 and $\geq$60$\textrm{cm}^2$/V-s in 1.5 and 10$\mu\textrm{m}$, respectively), low V$\sub$t/, ($\leq$1.52 and $\leq$ 2.75V in 1.5 and 10$\mu\textrm{m}$, respectively), low S$\sub$t/, and good ON-OFF characteristics in spite of temperature variation. Thus, poly-Si films formed by SPC can be applied for the application to poly-Si TFT liquid crystal display with peripheral integrated circuits.

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Explosion Hazards and Flame Velocity in Aluminum Powders (알루미늄 분체의 폭발위험성과 화염전파속도)

  • Han, Ou-Sup;Lee, Su-Hee
    • Journal of the Korean Institute of Gas
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    • v.16 no.5
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    • pp.7-13
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    • 2012
  • An experimental study has been done to investigate the explosion characteristics of aluminum powders with different sizes and concentrations in a 20 L spherical explosion vessel. Two different sizes of aluminum powder were used : $15.1{\mu}m$ and $34.8{\mu}m$ with a volume mean diameter. The results revealed that $15.1{\mu}m$ Al powder has a Lower explosion limit (LEL) of $40g/m^3$, a maximun explosion pressure ($P_{max}$) of 9.8 bar and a maximum rate of pressure rise ($[dP/dt]_{max}$) of 1852 bar/s, in $34.8{\mu}m$ Al powder, LEL of $70g/m^3$, $P_{max}$ of 7.9 bar and $[dP/dt]_{max}$ of 322 bar/s. The LEL of Al powders tended to increase with the increase of particle size. Also, it was found that the flame velocity calculated from the powder with $15.1{\mu}m$ was about 5 times higher than that of the powder of $34.8{\mu}m$.

Variations of pH Values and EC of Stemflow of 5 Tree Species in Chunchon Districts, Kangwon-do (강원도(江原道) 춘천시(春川市) 지역(地域)에 있어서 5개(個) 수종(樹種) 수간류(樹幹流)의 pH 및 전기전도도(電氣傳導度) 변화(變化))

  • Kim, Min Sik;Seomun, Won;Chun, Kun-Woo
    • Journal of Korean Society of Forest Science
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    • v.90 no.4
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    • pp.413-419
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    • 2001
  • This study investigated pH values and EC changes of rainfall and stemflow were investigated for 5 tree species such as Pinus koraiensis, P. densiflora, Larix leptolepis, Quercus variabilis and Q. mongolica in the Experiment Forests, College of Forest Science, Kangwon National University. The results were as follows; 1. Mean month pH values of stemflow were much lower than those of rainfall. Among tree species, pH values of the coniferous such as P. densiflora, P. koraiensis and L. leptolepis were lower those of the deciduous such as Q. mongolica and Q. variabilis. Mean month pH values of stemflow ranged from 3.23 to 5.03 (average : 4.23) for P. koraiensis, 3.57 to 5.16 (average : 4.36) for P. densiflora, 3.41 to 6.69 (average : 4.45) for L. leptolepis, 3.86 to 6.11 (average : 4.70) for Q. variabilis and 4.39 to 6.13 (average : 5.18) for Q. mongolica. 2. Mean month EC of the coniferous species was 1.5 to 2 times higher than that of the deciduous species. Mean month EC of stemflow for P. koraiensis, P. densiflora, L. leptolepis, Q. variabilis and Q. mongolica was ranged from 29.0 to $946.5{\mu}S/cm$(average : $258.5{\mu}S/cm$), 18.6 to $907.5{\mu}S/cm$(average : $220.3{\mu}S/cm$), 27.0 to $918.0{\mu}S/cm$(average : $216.2{\mu}S/cm$), 16.5 to $301.6{\mu}S/cm$(average : $125.4{\mu}S/cm$) and $25.2{\sim}225.7{\mu}S/cm$(average : $101.5{\mu}S/cm$), respectively. 3. Average pH values and EC were highly variable according to season. Average pH values appeared lower in spring than those in summer and autumn, but EC appeared higher in spring than those lower in summer and autumn. 4. According to precipitation, there was little difference in and pH values of stemflow the coniferous and the deciduous showed low pH valuess in the case of less than 50mm precipitation. However, EC of stemflow showed a difference depending on the precipitation, and EC of the coniferous and the deciduous was high more than $100{\mu}S/cm$ at the precipitation of less than 50mm.

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Power Loss and Electro-Magnetic Characteristics of Ni-Cu-Zn Ferrites (Ni-Cu-Zn페라이트의 損失과 磁性 特性)

  • Otsuki, E.;Kim, Jeong-Su
    • Resources Recycling
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    • v.13 no.6
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    • pp.37-42
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    • 2004
  • The power loss analysis was carried out for Ni-Cu-Zn ferrite sample with different content of NiO and ZnO. The power loss, Pcv decreases monotonically with increasing temperature and attains to a certain value at around 100~120 degrees Celsius. The frequency dependence of Pcv can be explained by Pcv~f$^n$, and n is independent of the frequency, f up to 1 MHz. The Pcv decreases with an increase in ZnO/NiO. The Pcv was separated to hysteresis loss(Ph) and residual loss(Pcv-Ph). The temperature characteristics and compositional dependence of Pcv can be attributed to the Ph, while Pcv-Ph is not affected by both temperature and ZnO/NiO. By analyzing temperature and composition dependence of Ph and initial permeability, ${\mu}_i$ like following equations could be formularized. ${\mu}_i{\mu}_0=I_s^2/(K_I+b{\sigma}_0{\lambda}_s)$ Wh=13.5(I$_s^2/{\mu}_i{\mu}_0)$ Where ${\mu}_0$ is permeability of vacuum, I$_s$ is saturation magnetization, K$_I$ is anisotropy constant, $s_0$ is internal heterogeneous stress, ${\lambda}_s$ is magnetostriction constant, b is unknown constant, and Wh is hysteresis loss per one cycle of excitation (Ph=Wh${\times}$f). Steinmetz constant of Ni-Cu-Zn ferrite, m=1.64~2.2 is smaller than that of Mn-Zn ferrites, which suggests the difference of loss mechanisms between these materials.

Effect of Fusion Procedure on the Development of Embryos Produced by Somatic Cell Nuclear Transfer in Hanwoo (Korean Cattle) (한우에서 융합방법이 체세포 핵이식 수정란의 발달에 미치는 영향)

  • Im, G.S.;Yang, B.S.;Park, S.J.;Chang, W.K.;Park, C.S.
    • Korean Journal of Animal Reproduction
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    • v.24 no.4
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    • pp.365-373
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    • 2000
  • The purpose of this study was to investigate the effects of the fusion pulses and fusion media on fusion rate and the development of embryos produced by somatic cell nuclear transfer in Hanwoo (Korean cattle). Nuclear donor cumulus and fetal fibroblast cells were cultured in Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum at 38.5$^{\circ}C$ in a humidified atmosphere of 5% $CO_2$in air. The in vitro matured oocytes were enucleated and then the isolated donor cells were introduced. The cumulus cell and cytoplast were fused using one pulse of 70 volts for 40$mutextrm{s}$, two pulses of 70 volts for 40$mutextrm{s}$ and one pulse of 180 volts for 15$mutextrm{s}$. The fetal fibroblast cell and cytoplast were fused using one pulse of 180 volts for 15$mutextrm{s}$ or 30$mutextrm{s}$. The cumulus cell and cytoplast were fused using mannitol and Zimmerman cell fusion medium (ZCFM) as a fusion medium. The fused embryos were activated after the fusion with 10 $\mu$M calcium ionophore for 5 min and 2 mM 6-dimethyl- aminopurine for 3 h. The nuclear transfer embryos were cultured in 500 ${mu}ell$ well of modified CR1aa supplemented with 3 mg/$m\ell$ BSA in th $\varepsilon$ four well dish cove red with mineral oil. After 3 days culture, culture medium was changed into modified CRlaa medium containing 1.5 mg/$m\ell$ BSA and 5% FBS for 4 days. The incubation environment was 5% $CO_2$, 5% $O_2$, 90% $N_2$ at 38.5$^{\circ}C$. When the cumulus cells were fused with enucleated oocytes by three different fusion pulses, one pulse of 180 volts for 15 $mutextrm{s}$ yielded the highest fusion rate and developmental rate to blastocyst among the pulses (P<0.05). When the fetal fibroblast cells were fused with enucleated oocytes, one pulse of 180 volts for 30$mutextrm{s}$ yielded significantly higher fusion rate compared with that for 15 $mutextrm{s}$(P<0.05). The present result indicates that the fusion rate between karyoplast and cytoplast was affected by the cell type and the optimal fusion condition was different according to cell type or size. When the fusion was conducted by the use of mannitol and ZCFM, the fusion rate was 71.2% and 65.8%, respectively. The developmental rates to blastocyst were 37.8% and 39.8%, respectively. There was no significant difference between two fusion media in the developmental rate of cumulus cell nuclear transfer embryos. These results indicate that optimal electric current should be selected according to cell type.

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Biological Characteristics and Growth of the Korean Freshwater Rotifer, Brachionus calyciflorus at Various Temperatures (한국 담수산 윤층 Brachionus calyciflorus의 생물학적 특징과 온도별 성장)

  • 강언종;이배익;김응오
    • Journal of Aquaculture
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    • v.10 no.4
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    • pp.449-456
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    • 1997
  • This study was concuted to determine the optimal conditions for raising the freshwater rotifer, Brachinus calyciflorus. The authors presented some biological informatin obtained from incubation experiment under the various controlled temperatures. Lorica size of the rotifer was divided into two groups : the length and the width for the S-type was $141.0\pm16.7\mu m$($110.1-182.5\;\mu m, n=44$)and $107.0\pm20.3\mu m\;(75.3-152.3\mu m, n=44)$, and those for the L-type was $262.8\pm15.2\mu m\;(234.4-288.6\mu m,\;n=20)\;and\;182.6\pm13.4\mu m (159.8-207.0\mu m,\;n=20$), respectively. The number of eggs being attached on the female varied from 1 to 11 at various culture conditions. Egg type was divided into two groups, large and small. Large and small egg was measured in its major axis as 85a.7-107.8$\mu$m and 55.1-65.2$\mu$m for S-type, and 104.9-121.8 $\mu$m and 62.8-89.1$\mu$m for L-type respectively. The maximum density was reached at 4th day after incubation. The density was 583.9 rotifers/$m\ell$ for $25^{\circ}C$-experimental. group and 421.3 rotifers/$m\ell$ for $22^{\circ}C$-experimental. group respectively. In the case of $28^{\circ}C$-experimental. group, it suddenly decreased into 4.7 rotifers/$m\ell$ at 1st day after incubations and did not recover to its initial density. The maximum rate of increase of populatin per day was reached 0.802 for $22^{\circ}C$-experimental. group at day 2 and fluctuated thereafter. For $25^{\circ}C$-experimental. group it increased to 0.964 at day 3 of incubation and then declined. And the egg ratio of female was reached the maximum of 0.614 for 22$^{\circ}C$- at 3rd day and 0.772 for $25^{\circ}C$-experimental. group at 4th day of incubation.

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Studies on High Speed Addressing Driving Scheme using the Priming Effect in Plasma Display Panel (하전 입자 효과를 이용한 Plasma Display Panel의 고속 구동 파형에 관한 연구)

  • Shin, Bhum-Jae;Park, Sang-Sik
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.23 no.2
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    • pp.45-52
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    • 2009
  • This study is related to the realization of high speed address driving method for Full-HD PDP. The new self-priming addressing(SPA) driving scheme was proposed to improve an address discharge time lag, which utilizes the priming effect maintaining the priming discharge during an address period. In this study, the basic characteristics of the priming ramp discharge were investigated and optimize the reset pulse and priming pulse. It is noted that the address discharge time lag is significantly improved from 1.2[${\mu}s$] to 0.8[${\mu}s$] when the slope of the priming ramp pulse is below 0.1[$V/{\mu}s$].