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http://dx.doi.org/10.11626/KJEB.2017.35.4.622

Isolation, Regeneration and the Maturation Induction of Free-Living Gametophytes of Undaria pinnatifida (Phaeophyceae)  

Yoo, Hyun Il (Seaweed Research Center, National Institute of Fisheries Science)
Kim, Su Hong (Seaweed Research Center, National Institute of Fisheries Science)
Lee, Gi hyun (Seaweed Research Center, National Institute of Fisheries Science)
Ha, Dong Soo (Seaweed Research Center, National Institute of Fisheries Science)
Hwang, Eun Kyoung (Seaweed Research Center, National Institute of Fisheries Science)
Publication Information
Korean Journal of Environmental Biology / v.35, no.4, 2017 , pp. 622-630 More about this Journal
Abstract
The induction of regeneration and the maturation of the free-living gametophytes of Undaria pinnatifida were studied at temperatures(5, 10, 15 and $20^{\circ}C$), irradiance (5, 10, 20 and $40{\mu}mol\;photons\;m^{-2}\;s^{-1}$) and photoperiods (14 : 10, 12 : 12 and 10 : 14 h L: D). Female gametophyte fragments were maintained in active regeneration without them reaching sexual maturity under conditions of $15^{\circ}C$, $20{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 10 : 14 h (L : D); by contrast, the conditions for male gametophytes were slightly different at $15^{\circ}C$, $5{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 14 : 10 h (L: D). The sexual maturation of female and male gametophytes took place under $5^{\circ}C$, $20-40{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 10 : 14 h (L : D) and $15^{\circ}C$, $40{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 10 : 14 h (L : D), respectively. These results provide basic information for controlling the regeneration and maturation of free-living gametophytes for srain improvement as well as the cross breeding of U. pinnatifida.
Keywords
free-living gametophyte; maturation; regeneration; Undaria pinnatifida;
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Times Cited By KSCI : 6  (Citation Analysis)
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