• Journal of Applied Biological Chemistry
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• v.57 no.4
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• pp.295-299
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• 2014

The mulberry (Morus alba L.) root barks were extracted with 80% aqueous methanol at room temperature. The concentrated extract was partitioned as ethyl acetate (EtOAc), n-BuOH, and $H_2O$ fractions. From the EtOAc fraction, five triterpenoids were isolated through the repeated silica gel and octadecyl $SiO_2$ column chromatographies. According to the results of physico-chemical and spectroscopic data including nuclear magnetic resonance, mass spectrometry, and infrared, the chemical structures of the triterpenoids were respectively determined as ${\alpha}$-amyrin (1), ${\alpha}$-acetyl amyrin (2), lupeol (3), betulinic acid (4), and glutinol (5). Compounds 1, 3, and 5 were isolated for the first time from the mulberry root bark.

• Journal of Sericultural and Entomological Science
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• v.47 no.2
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• pp.56-61
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• 2005

New mulberry cultivar, Shinsungppong which was resistant to main mulberry disease was bred and resistered as a commercial cultivar. Local adaptability test has been carried out at three places (Suwon, Kongju, and Jinju) for four years since 2001. Leaf quality was tested by silkworm rearing for two silkworm rearing seasons (spring and autumn) at Suwon in 2000. Shinsungppong was recognized to be superior by 7% in productivity to control cultivar, Sangilppong. The new cultivar, Shinsungppong, has following agronomic characteristics as compared with Sangilppong, which is known as a resistant cultivar to mulberry disease in Korea. It is belonging to Morus alba L. with mixed shapes from the ovate to 5 lobated leaf shape, sprouting in spring nearly the same to the Sangilppong (Morus alba L.), high leaf yielding by 7% compared to Sangilppong (Morus alba L.). It is adaptable to everywhere except the places where cold damage frequently in Korea. Sangilppong is recommended to rear silkworms not only in spring but also in autumn besides for young silkworms.

• Journal of Sericultural and Entomological Science
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• v.46 no.1
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• pp.12-17
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• 2004

New triploid mulberry cultivar, Suilppong was bred among selected lines from F$_1$ seedlings obtained by crossing the female, artificial tetraploid No. 411 which obtained from Chungilppong seedlings, and the male, Kugsang 21. Local adaptability test has been carried out at four places (Suwon, Kongju, Jeonju and Jangsung) for five years since 1997. Leaf quality was tested by silkworm rearing for two silkworm rearing seasons (spring and autumn) at Suwon in 2000. Suilppong was recognized to be superior in productivity to control cultivar, Kaeryangppong. The new cultivar, Suilppong, has following agronomic characteristics as compared with Kaeryangnppong, which is leading cultivar widely cultivating in Korea. It is belonging to Morus alba L. with mixed shapes from the ovate to 5 lobated leaf shape, sprouting in spring nearly the same to the Kaeryangppong (Morus alba L.), high leaf yielding of 20% compared to Kaeryangppong (Morus alba L.). It is adaptable to everywhere except the places where cold damage occurs frequently in Korea. Suilppong is recommended to rear silkworms not only in spring but also in autumn besides for young silkworms.

• Natural Product Sciences
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• v.15 no.1
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• pp.27-31
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• 2009

The present study was performed to investigate the binding affinity of the ethanol extract from the leaves of Morus alba (EMA) and some EMA related plant materials (EMA-D, EMA-DM) for melanin-concentrating hormone-1 receptor (MCH-1) and also to examine the antagonistic effect of them for the recombinant MCH-1 receptor expressed in CHO cells. EMA, dichloromethane fraction (EMA-D) and EMA-DM exhibited high affinity for mammalian MCH receptor in receptor binding assays ($IC_{50}$ value: 2.3, 1.6 and $1.0{\mu}g/ml$, respectively). Other plant materials (MMA-D, MMA-DM) obtained from methanol extracts from the leaves of Morus alba (MMA) also exhibited high affinity for mammalian MCH receptor, even though the $IC_{50}$ values of them were lower than those of EMA-D and EMA-DM. In Chinese hamster ovary (CHO) cells expressing human MCH-1, EMA-DM and EMA-D significantly inhibited MCH-induced intracellular $Ca^{2+}$ increase ($IC_{50}$ values: 16.5 and $22.7{\mu}g/ml$, respectively). These results clearly indicate that the ethanol extract from the leaves of Morus alba (EMA) and some EMA related plant materials (EMA-D, EMA-DM) are novel selective MCH-1 receptor antagonist, respectively.

• Journal of Sericultural and Entomological Science
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• v.44 no.2
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• pp.45-50
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• 2002

New mulberry cultivar, Hansungppong, was bred among selected lines from F1 seedlings obtained by crossing the female, Yongcheonppong, and the male, Kugsang 21. Local adaptability test has been carried out at two places (Suwon and Chunchun) for four years since 1995. Leaf quality was tested by silkworm rearing for two silkworm rearing seasons (spring and autumn) at Suwon in 1998. Hansungppong was recognized to be superior in productivity and cold resistance to control cultivar, Yongcheonppong. The new cultivar, Hansungppong, has following agronomic characteristics as compared with Yongcheonppong, which was strongest leading cultivar against cold injury in Korea. It is belonging to Morus alba L. with the ovate leaf shape, sprouting in spring nearly the same to the Yongcheonppong (Morus alba L.), high leaf yielding of 10% compared to Yongcheonppong (Morus alba L.) and the high degree of cold resistance. It is adaptable to everywhere in Korea. Hansungppong is recommended to rear silkworms not only in spring but also in autumn besides for young silkworms.

• Korean Journal of Plant Resources
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• v.26 no.3
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• pp.397-402
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• 2013

Morus Folium (Sang-yeop in Korean) is one of the most important Oriental medicinal plants. In Korea, both M. alba and M. cathayana are regarded as the botanical sources for Morus Folium. In order to discriminate M. alba and M. cathayana from their adulterant, M. tricuspidata, mitochondrial NADH dehydrogenase subunit 7 (nad7) intron 2 region was targeted for molecular analysis with universal primers. DNA polymorphisms, including SNP sites, insertions, and deletions, were detected among these three species sequencing data. Based on these DNA polymorphisms, specific primers were designed for the three species respectively. Multiplex PCR was conducted for molecular authentication of M. alba, M. cathayana, and M. tricuspidata with specific primers. The present results indicate that it is possible to identify Morus Folium from its adulterant using mitochondrial nad7 intron 2 region. The established multiplex-PCR system was proved to be effective for identification of Morus Folium. The results indicate that mitochondrial introns can be used for inter-specific polymorphic study, and the described method can be applied for molecular identification of medicinal materials.

• The Korean Journal of Food And Nutrition
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• v.11 no.5
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• pp.576-579
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• 1998

Water extract of mulberry tree barks(Morus alba Linne) was studied for its proteolytic activity. Protein content of the extract was 1.12mg/ml and its specific activity was 5.14U/ml. The enzyme was active on various proteins : the relative acitities were 100 for casein, 63 for albumin, 58 for collagen, 45 for hemoglobin and 36 gelatin, respectively. There suggested that the ability of the enzyme to hydrolyze meat was relatively high since those are major meat proteins. Optimum pH and temperature for proteolytic activity were : pH 6.0 and 6$0^{\circ}C$. And the enzyme was stable at the pH range of 6.0 to 7.0 and temperature between 50 and 8$0^{\circ}C$. Apparent proteolytic activities could support some scientific grounds of traditional application of mulberry tree barks to home cooking for meat tenderization.

• YAKHAK HOEJI
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• v.54 no.5
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• pp.398-402
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• 2010

[ ${\alpha}$ ]Glucosidase inhibitor is a target in the treatment of type II diabetes through the mainly inhibition of glucose levels after meals. In this study, we purified steppogenin and oxyresveratrol from the stem of Morus alba L. and examined their inhibitory activity against yeast ${\alpha}$-glucosidase. Steppogenin and oxyresveratrol were inhibited yeast ${\alpha}$-glucosidase in a dose dependent manner. The $IC_{50}$ activities (50% inhibition) were 34.4 and 9.3 ${\mu}M$, respectively. The kinetic inhibition of steppogenin showed noncompetitive inhibition ($K_m:1.1{\times}10^{-3}M$; $K_i:1{\times}10^{-5}M$), meanwhile oxyresveratrol showed competitive inhibition ($K_m:4.3{\times}10^{-3}M$; $K_i:3.4{\times}10^{-6}M$) against yeast ${\alpha}$-glucosidase. These results indicate that steppogenin and oxyresveratrol are noncompetitive and competitive inhibitors, respectively, against yeast ${\alpha}$-glucosidase.

• Natural Product Sciences
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• v.8 no.4
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• pp.129-132
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• 2002

The MeOH extract of the twig of Morus alba L. (Moraceae) inhibited strong lipid peroxidation activity. In order to find out active principle from the plant, acivity-guided fractionation was performed and five antioxidant compounds were isolated. Their chemical structures were identified as 6-geranylapigenin (1), 6-geranylnorartocarpetin (2), resveratrol (3), oxyresveratrol (4) and quercetin (5) by physicochemical and spectrometric methods. Compounds 1-5 significantly inhibited lipid peroxidation in rat brain homogenate $(IC_{50}\;values\;of\;3.37,\;3.74,\;0.23,\;0.29\;and\;0.06\; {\mu}M,\;respectively)$.

• Journal of Sericultural and Entomological Science
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• v.3
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• pp.73-74
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• 1963

우리나라 Morus(뽕나무속)에 대한 분류학적인 연구는 지금으로부터 약 40 여년전부터 시작되어 현재는 거의 정리되었다고 볼 수 있다. 우선 우리나라에 자생되는 각종에 대하여 개별적인 분류지리학적면을 탐견 약기하여 보기로 한다. Morus alba L. 즉 재배종이며 보편적인 "뽕나무"로 알려져 있는 것이며 수많은 변, 품종들이 여기서 파생되고 있다. 한국산 야생 Morus를 크게 나누어 세가지 계통으로 구분할 수있다. 즉 I. Morus bombycis Koidzumi. 산뽕나무(Yamaguwa). II. Morus mongolica Schneider. 몽고뽕나무(Mongoguwa). III. Morus tiliaefolia Makino. 참털뽕나무(Keguwa). (중략)