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http://dx.doi.org/10.3839/jabc.2014.046

Isolation and Identification of Triterpenoids from the Mulberry (Morus alba) Root Bark  

Jung, Jae-Woo (Graduate School of Biotechnology & Department of Oriental Medicinal Materials and Processing, Kyung Hee University)
Park, Ji-Hae (Graduate School of Biotechnology & Department of Oriental Medicinal Materials and Processing, Kyung Hee University)
Jung, Ye-Jin (Graduate School of Biotechnology & Department of Oriental Medicinal Materials and Processing, Kyung Hee University)
Lee, Chang-Ho (Division of Matabolism and Functionality Research, Korea Food Research Institute)
Han, Daeseok (Division of Matabolism and Functionality Research, Korea Food Research Institute)
Baek, Nam-In (Graduate School of Biotechnology & Department of Oriental Medicinal Materials and Processing, Kyung Hee University)
Publication Information
Journal of Applied Biological Chemistry / v.57, no.4, 2014 , pp. 295-299 More about this Journal
Abstract
The mulberry (Morus alba L.) root barks were extracted with 80% aqueous methanol at room temperature. The concentrated extract was partitioned as ethyl acetate (EtOAc), n-BuOH, and $H_2O$ fractions. From the EtOAc fraction, five triterpenoids were isolated through the repeated silica gel and octadecyl $SiO_2$ column chromatographies. According to the results of physico-chemical and spectroscopic data including nuclear magnetic resonance, mass spectrometry, and infrared, the chemical structures of the triterpenoids were respectively determined as ${\alpha}$-amyrin (1), ${\alpha}$-acetyl amyrin (2), lupeol (3), betulinic acid (4), and glutinol (5). Compounds 1, 3, and 5 were isolated for the first time from the mulberry root bark.
Keywords
Morus alba L.; mulberry root bark; nuclear magnetic resonance; triterpenoid;
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Times Cited By KSCI : 5  (Citation Analysis)
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