• Journal of Plant Biotechnology
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• v.42 no.1
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• pp.60-70
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• 2015

The aim of this study was to investigate whether fourier transform infrared (FT-IR) spectroscopy can be applied to simultaneous determination of fatty acids contents in different soybean cultivars. Total 153 lines of soybean (Glycine max Merrill) were examined by FT-IR spectroscopy. Quantification of fatty acids from the soybean lines was confirmed by quantitative gas chromatography (GC) analysis. The quantitative spectral variation among different soybean lines was observed in the amide bond region ($1,700{\sim}1,500cm^{-1}$), phosphodiester groups ($1,500{\sim}1,300cm^{-1}$) and sugar region ($1,200{\sim}1,000cm^{-1}$) of FT-IR spectra. The quantitative prediction modeling of 5 individual fatty acids contents (palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid) from soybean lines were established using partial least square regression algorithm from FT-IR spectra. In cross validation, there were high correlations ($R^2{\geq}0.97$) between predicted content of 5 individual fatty acids by PLS regression modeling from FT-IR spectra and measured content by GC. In external validation, palmitic acid ($R^2=0.8002$), oleic acid ($R^2=0.8909$) and linoleic acid ($R^2=0.815$) were predicted with good accuracy, while prediction for stearic acid ($R^2=0.4598$), linolenic acid ($R^2=0.6868$) had relatively lower accuracy. These results clearly show that FT-IR spectra combined with multivariate analysis can be used to accurately predict fatty acids contents in soybean lines. Therefore, we suggest that the PLS prediction system for fatty acid contents using FT-IR analysis could be applied as a rapid and high throughput screening tool for the breeding for modified Fatty acid composition in soybean and contribute to accelerating the conventional breeding.

• Journal of Plant Biotechnology
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• v.38 no.2
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• pp.176-185
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• 2011

Rice is one of the most important crop in the world, in particular for food resources. With its small genome size of 383 Mb, the Oryza sativa is a model plant for genome research. Indeed, it's grain provides human with a source of carbohydrates and proteins. Rice grain has relatively low protein contents (around 8%) compared to other legume seeds (around 40%). Osborne classified seed proteins into water soluble albumin, salt soluble globulin, alcohol soluble prolamin and acidic/alkaline solution soluble glutelin. Glutelin and prolamin are the major storage proteins in rice. For the gene expression study of seed storage proteins, we analyzed 33,192 EST clones at immature stages in a rice cultivar (Oryza sativa L. cv. 'Ilpum'). Based on the expression analysis, we cloned 11 glutelin genes and figured out the 8 genes are located on Chromosome 2. The expression of glutelin genes appears to be about 28.2% of total level in immature seeds. Interestingly, glu-04 is duplicated as inverted sequences on the same chromosomes as far 4.5 kb. Our results indicate that glutelin genes, evolutionarily, were replicated on the chromosome and thus expressed as specific manners. In a whole protein composition analysis, glu05 (type B7) contains the highest lysin contents (4.51%) among the 11 rice glutelin genes. It will be an interesting future work to increase lysin contents by the gene overexpressor strategy with the aim of improved diet nutritionally fortified.

• Economic and Environmental Geology
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• v.30 no.3
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• pp.249-262
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• 1997

The Sr and Nd isotopic compositions of two foliated granitic plutons located in the Chonju and Sunchang area were determined in order to reconfirm the intrusion ages of granitoids and to study the sources of granitic magmas. The best defined Rb-Sr isochron for the whole rock samples of the Chonju foliated granite (CFGR) give an age of $284{\pm}12Ma$, suggesting early Permian intrusion age. In contrast, the whole rock Rb-Sr data of the Sunchang foliated granite (SFGR) scatter widely on the isochron diagram with very little variation in the $^{87}Rb/^{86}Sr$ ratios and, therefore, yield no reliable age information. Futhermore they show the concordance of mineral and whole rock Rb-Sr isochron and divide into two linear groups with roughly the same slopes and significantly different $^{87}Sr/^{86}Sr$ ratios, indicating some kind of Rb-Sr distortion in whole rock scale and a difference in source material and/or magmatic evolution between two subsets. The reconstructed isochrons of 243 Ma, which was defined from the proposed data by the omission of one sample point with significantly higher $^{87}Rb/^{86}Sr$ ratio than the others, and 252 Ma, from the combined data of it and some of this study, strongly suggest the possibility that the SFGR was intruded appreciably earlier than had previously been proposed, although the reliability of these ages still questionable owing to high scatter of data points and, therefore, further study is necessary. All mineral isochrons for the investigated granites show the Jurassic to early Cretaceous thermal episode ranging from 160 Ma to 120 Ma Their corresponding initial $^{87}Sr/^{86}Sr$ ratios correlate well with their whole rock data, indicating that the mineral Rb-Sr system of the investigated granites was redistributed by the postmagmatic thermal event during Jurassic to early Cretaceous. The initial ${\varepsilon}Sr$ values for the CFGR (64.27 to 94.81) tend to be significantly lower than those for the SFGR (125.43 to 167.09). Thus it is likely that there is a marked difference in the magma source characteristics between the CFGR and the SFGR, although the possibility of an isotopic resetting event giving rise to a high apparent initial ${\varepsilon}Sr$ in the SFGR can not be ruled out. In contrast to ${\varepsilon}Sr$, both batholiths show a highly resticted and negative values of initial ${\varepsilon}Nd$, which is -14.73 to -19.53 with an average $-16.13{\pm}1.47$ in the CFGR and -14.78 to -18.59 with an average $-17.17{\pm}1.01$ in the SFGR. The highly negative initial ${\varepsilon}Nd$ values in the investigated granitoids strongly suggest that large amounts of recycled old continental components have taken part in their evolution. Furthermore, this highly resticted variation in ${\varepsilon}Nd$ is significant because it requires that the old crustal source material, from which the granitoid-producing melts were generated, should have a reasonably uniform Nd isotopic composition and also quit similar age. Calculated T2DM model ages give an average of $1.83{\pm}0.25Ga$ for CFGR and $1.96{\pm}0.19Ga$ for SFGR, suggesting the importance of a mid-Proterozoic episode for the genesis of two foliated granites. Although it is not possible to determine precisely the source rock compositions for the investigated foliatic granites, the Sr-Nd isotopic evidences indicate that midcrustal or less probably, a lower crustal granulitic source could be the most likely candidate.

• Economic and Environmental Geology
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• v.42 no.4
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• pp.289-300
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• 2009

The Geodo mine area, had been developed for Fe and Cu ores since 1963 and abandoned in recent decades, is located in the central part of the Taebaeksan mineralized district. This area comprises of the Jangsan, Myobong, Pungchon, Hwajeol, Dongjeom, and Dumugol Formations in ascending stratigraphic order. These Formations were intruded by the Cretaceous Eopyeong granitoids that appears to produce the Geodo skarn. Their compositions are relatively oxidized quartz monzodiorite to granodiorite (magnetite series, $Fe_2O_3/FeO=0.3{\sim}1.1$). Mineralizations related skarn deposit occur in the Myobong, Pungchon, and Hwajeol Formations. The proximal skarn is zoned from andraditic garnet ($Ad_{44-95}Gr_{1-53}$) predominant adjacent to the Eopyeong granitoids to diopsidic pyroxene ($Hd_{10-100}Di_{0-89}$) predominant away from the one. The differential proportion of garnet and pyroxene is generated by water/rock ratio and their source, such as magmatic and meteoric water. This is useful tool for assessment the overall oxidation state of the entire skarn system. Gold occurs in proximal red to brownish garnet skarn, and genetically associated with Bi- and Te-bearing minerals. Skarn deposit developed in the Geodo mine area is considered as oxidized Au skarn category, based on chemical composition of the Eopyeong granitoids, zonation of skarn, and gold occurrences. Garnet-rich skarn zone will be the main target for exploration of gold in the study area. However, it is needed to the detailed survey on vertical zonation of this area as well as lateral zonation. The result of this survey would provide an important basis for the exploration of the skarn Au deposit in the Geodo mine area.

• Korean Journal of Ecology and Environment
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• v.49 no.1
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• pp.11-21
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• 2016

Cymbidium kanran Makino is being threatened in its own habitats due illegal collecting and habitat changes by vegetation growth along historical landuse change. In this study, we established habitat restoration model for conservation of C. kanran based on ecological diagnosis. Through exploration to Jeju Island in 2014 and 2015, we identified 27 unknown habitats of C. kanran and in there, abiotic variables and vegetation structure and composition were quantified. Altitudinal distribution of C. kanran was between 200 m~700 m a.s.l. and compared to distribution in 2004, Area of Occupation (AOO) decreased at 82%. Specific habitat affinity was not observed by evenly found in mountain slope and valley and summergreen and evergreen broadleaved forests, but likely more abundant in valley habitats with higher soil and ambient moisture. Total of 96 individual of C. kanran was observed with an average density of $942.6individuals\;ha^{-1}$. The plants showed relatively short leaf length (average=$10.7cm{\pm}1.1cm$) and small number of pseudo bulbs ($1.2{\pm}0.2$). Flowering and fruiting individuals were not observed in field. C. kanran was classified into endangered plant species as CR (Critically Endangered) category by IUCN criteria. Phenotypic plasticity of C. kanran was likely support to sustain in more shaded habitat environment and recent habatat changes to closed canopy and low light availability may exhibit negatively effects to C. kanran's life history. Restoring C. kanran habitat should create open environment as grassland and low woody species density.

• Korean Journal of Environment and Ecology
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• v.30 no.2
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• pp.185-198
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• 2016

Korea has put in significant efforts to increase the number of wildlife crossings between fragmented habitats to prevent loss of biodiversity and to encourage the habitat connectivity in Korea. However, there is a lack of biological data on the effect of vegetation structure in these wildlife crossings and guidelines for design and management of wildlife crossing structures in Korea. Therefore, we selected ground beetle assemblages as model organisms to compare the effect of vegetation structure in wildlife crossings, i.e. bare ground- and shrub-type corridors, in agro-forested landscapes. For this study, 4,207 ground beetles belonging to 33 species were collected through pitfall trapping along the northern forest-corridor-southern forest transects from late April to early September in 2015. Dominant species, abundance, and species richness of ground beetles were significantly higher in the shrub-type corridors than the bare ground-type corridors. Also, the species composition of bare ground-type corridor was significantly different compared to the other habitats such as shrub-type corridor and forests. Similarly, environmental variables were also influenced by vegetation management regimes or trap locations. Collectively, our study clearly indicates that the movement of forest associated ground beetles between forest patches can increase as the vegetation in wildlife crossings becomes complex. Although further studies are needed to verify this, there are indications that the current wildlife crossings that comply with the guidelines may be unfriendly to the movement of ground dwelling arthropods as well as ground beetles. To enhance the ecological function of wildlife crossings, the guidelines need to be rectified as follows: 1) Shrubs or trees should be planted along the corridor verges to provide refuge or movement paths for small mammals and ground dwelling arthropods, and 2) Open spaces should be provided in the middle of the corridors to be used as a path for the movement of large mammals.

• Journal of Korean Home Economics Education Association
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• v.18 no.3 s.41
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• pp.61-79
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• 2006

The purpose of this research was to evaluate concretely how the 7th Technology Home Economics(HE) curriculum was operated In school fields by using context evaluation, input evaluation using the CIPP model focusing HE. In this research. a questionnaire survey was done targeting HE teachers in charge of technology and home economics subject for the 7th curriculum implementation at 250 randomly selected middle schools in Gpoungsangnamdo, and 109 returned questionnaire papers were used for the final analysis. The frequency, percent, mean and standard deviation of these data were obtained by using the SPSS 10.0 for Windows Program. The results of this research were outlined below: First, with a regard to the context evaluation (evaluating educational contexts), the requirement degree of seeking for career appeared to be the highest at the requirement survey of the education goal and character of HE area, and the requirement degree of elevating understanding home living' were very low. Interest and concern of students towards these subject as well as the self-confidence of HE teachers in charge were low. Second, as for the input evaluation (evaluating education plans), time allocation, content level, and content amount were not regarded to be not proper, and the composition of HE educational curriculums and the class guideline of teachers also were not so faithful from the aspect of school level. Among surveyed items, establishing plans in school level towards evaluation was relatively sincere, but discussions about curriculums were not so faithful, suggesting various kinds of content are not fully discussed. As shown by the results above, it is difficult to say that the operation of the 7th HE educational curriculums has secured its position perfectly. It is necessary to examine systematically the educational goals. character of the 7th HE educational curriculum and the appropriateness of curriculum content, and to seek various methods to elevate the self confidence of teachers and the interests of students. Because how to operate national level educational curriculums in school levels with self-regulation and elasticity will be an important variable in realizing educational goals, teachers, the main body of educational curriculums and direct performers, should establish and perform educational plans in school levels to elevated the satisfaction of teachers and educational effects for normally operating educational curriculums. In addition, educational curriculums should be operated and evaluated mainly by teachers at school fields to make it possible to confirm and check the achievement of the goals of educational curriculums qualitatively.

• Journal of the Korean Institute of Landscape Architecture
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• v.38 no.3
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• pp.41-52
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• 2010

The purpose of this paper is to evaluate visitor satisfaction(VS) in waterfront parks in terms of resources, facilities, embodiment of theme(ET), site composition(SC), relaxation activity space(RAS), and dynamic activity space(DAS), which are supply-side components in the planning process of waterfront parks, in order to answer the research question; 'How is visitor satisfaction of waterfront parks determined?' After reviewing the literature on parks and the building process of waterfront parks in Korea, we constructed a conceptual framework and have ascertained a research hypothesis. We had obtained data through a questionnaire survey from 327 visitors at waterfront parks, based on the quota sampling method. We have analyzed the data using the path analysis method. We found that: 1) The direct effects of resources and facilities on VS turned out to be 0.273 and 0.306, respectively while the indirect effects are 0.114, 0.170, respectively. 2) The direct effects of SC, as a component of the planning process on VS, turned out to be 0.243 while that of ET had no affect on VS. The indirect effect of ET and SC on VS turned out to be 0.059 and 0.018, respectively. 3) The direct effects of RAS on VS turned out to be 0.129 while the indirect effects of RAS and DAS on VS turned out to be 0.002 and 0.017, respectively. 4) The size of causal effect, in order, were facilities, resources, SC, RAS, ET, and DAS. 5) Resources and facilities, as a park foundation, compose 64.84 percent of total causal effect while ET and SC have 24.04 percent and RAS and DAS have 11.12 percent, respectively. These research results imply that: 1) Existing waterfront parks should be regenerated with the embodiment of water related theme and with improved facilities for RAS and visitor programs and/or facilities for DAS. 2) The relationship among ET, SC, RAS and DAS should be increased for a significant improvement of VS, and 3) A process-oriented approach turned out to be highly useful for the development of substantive theory and methodology. It is recommended that a structural equation model on waterfront parks be developed using more empirical data and this approach be widely applied for testing its validity.

• Management & Information Systems Review
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• v.32 no.5
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• pp.69-88
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• 2013

This study aimed to analyze the effects that the exchange relationship among employees engaged in the wedding industry in Busan, Ulsan and Gyeongnam area exerted on job satisfaction and voluntary service intentions. For this purpose, a theoretical model was presented based on previous studies and the relationship among variables was analyzed based on the analysis results through a survey. There have recently been great difficulties in the whole wedding industry due to the global economic depression and the low birthrate. Moreover, it is hard to optimize the future business environment. In particular, the low birthrate has weakened the demand base of the wedding industry. As a result, it is expected that the competition among companies will be more fierce in the wedding industry. Employees' voluntary service intention become much more important to overcome difficulties in this industry. Employees' voluntary service intentions can be possible when their job satisfaction is achieved. Many studies report the result that this job satisfaction can naturally be achieved when the high quality exchange relationship among a leader, team members (co-workers) and customers is formed. It is viewed that employees' voluntary service intentions should be induced through job satisfaction to improve the profitability through the efficient operation and management of the system. A leader of the organization should make an effort to form the high-quality exchange relationship with members, support so that team members(coworkers) and other employees can get along together and the teamwork can be improved and induce them to improve a high level of service quality with friendly customer services. In spite of the significance of the study discussed above, there are the following limitations in this study. There is a limit to a certain extent in generalizing study conclusions because the study was limited to the Busan, Ulsan and Gyeongnam area. If practical surveys which full- and part-time employees are included in the composition of samples are conducted for wedding suppliers in the country in the future, the quality of study will be able to improve.

• Korean Journal of Food Science and Technology
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• v.11 no.1
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• pp.26-31
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• 1979

Free amino acids and total free sugar of kimchi were identified. Free amino acids of kimchis were extracted by 80% ethanol and isolated by ion exchange chromatography. Identification and quantitative determination of individual free amino acids were performed by amino acid autoanalyzer. Free sugar of kimchis was extracted by aqueous ethanol and isolated by ion exchange chromatography. Quantitative determination of it was perfermed by spectrophotometer. The results are summarized as follows: 1. Lysine, histidine, arginine, tryptophan, aspartic acid, threonine, glutamic acid, proline, glycine, alanine, cysteine, valine, methionine, leucine, isoleucine, tyrosine and phenylalanine were found in all kimchis. 2. The change of free amino acid composition during fermentation of kimchis was not observed, but the amount of total free amino acids of fermented kimchi decreased as compared with those of raw kimchi. 3. In kimchi containing 10 ml of fermented anchovy solution/100 g of chinese cabbage, the amount of total free amino acids was more than that of fermented salt kimchi and the characteristic flavor of it was attributed to such amino acids as lysine, aspartic acid, glutamic acid, valine, methionine, isoleucine and leucine. 4. Large amount of free sugar in raw salt kimchi decreased during fermentation, but, after fermentation, significant difference of free sugar content between salt kimchi and kimchi containing fermented anchovy solution was not observed.