Browse > Article
http://dx.doi.org/10.5010/JPB.2011.38.2.176

Structural and expression analysis of glutelin genes in Oryza sativa L.  

Yoon, Ung-Han (Genomics Division, National Academy of Agricultural Science, RDA)
Kim, Chang-Kug (Genomics Division, National Academy of Agricultural Science, RDA)
Lee, Gang-Seob (Genomics Division, National Academy of Agricultural Science, RDA)
Hahn, Jang-Ho (Genomics Division, National Academy of Agricultural Science, RDA)
Lee, Jeong-Hwa (Genomics Division, National Academy of Agricultural Science, RDA)
Kim, Yeon-Ki (Bioscience and Bioinformatics Division, MyongJi University)
Ji, Hyeon-So (Genomics Division, National Academy of Agricultural Science, RDA)
Mun, Jeong-Hwan (Genomics Division, National Academy of Agricultural Science, RDA)
Lee, Tae-Ho (Bioscience and Bioinformatics Division, MyongJi University)
Kim, Tae-Ho (Genomics Division, National Academy of Agricultural Science, RDA)
Publication Information
Journal of Plant Biotechnology / v.38, no.2, 2011 , pp. 176-185 More about this Journal
Abstract
Rice is one of the most important crop in the world, in particular for food resources. With its small genome size of 383 Mb, the Oryza sativa is a model plant for genome research. Indeed, it's grain provides human with a source of carbohydrates and proteins. Rice grain has relatively low protein contents (around 8%) compared to other legume seeds (around 40%). Osborne classified seed proteins into water soluble albumin, salt soluble globulin, alcohol soluble prolamin and acidic/alkaline solution soluble glutelin. Glutelin and prolamin are the major storage proteins in rice. For the gene expression study of seed storage proteins, we analyzed 33,192 EST clones at immature stages in a rice cultivar (Oryza sativa L. cv. 'Ilpum'). Based on the expression analysis, we cloned 11 glutelin genes and figured out the 8 genes are located on Chromosome 2. The expression of glutelin genes appears to be about 28.2% of total level in immature seeds. Interestingly, glu-04 is duplicated as inverted sequences on the same chromosomes as far 4.5 kb. Our results indicate that glutelin genes, evolutionarily, were replicated on the chromosome and thus expressed as specific manners. In a whole protein composition analysis, glu05 (type B7) contains the highest lysin contents (4.51%) among the 11 rice glutelin genes. It will be an interesting future work to increase lysin contents by the gene overexpressor strategy with the aim of improved diet nutritionally fortified.
Keywords
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Tanaka K, Sugimoto T, Ogawa M, Kasi Z. (1980) Isolation and characterization of two types of protein bodies in the rice endosperm. Agric. Biol. Chem 44:1633-39   DOI
2 Wakasa Y, Yang L, Hirose S, Takaiwa F. (2009) Expression of unprocessed glutelin precursor alters polymerization without affecting trafficking and accumulation. J Exp Bot 60: 3503-11   DOI
3 Wen TN, Luthe DS. (1985) Biochemical characterization of rice glutelin. Plant Physiol 78:172-77   DOI
4 Wu CY, Suzuki A, Washida H, Takaiwa F. (1998) The GCN4 motif in a rice glutelin gene is essential for endosperm-specific gene expression and is activated by Opaque-2 in transgenic rice plants. Plant J 14:673-83   DOI
5 Xu JH, Messing J. (2008) Organization of the prolamin gene family provides insight into the evolution of the maize genome and gene duplications in grass species. Proc Natl Acad Sci U S A 105:14330-5   DOI
6 Xu JH, Messing J. (2009) Amplification of prolamin storage protein genes in different subfamilies of the Poaceae. Theor Appl Genet 119:1397-412   DOI
7 Yoon UH, Lee GS, Lee JS, Hahn JH, Kim CK, Kikuchi S, Satoh K, Kim JA, Lee JH, Lee TH, Kim YH. (2009a) Structural analysis of seed developmental stage ESTs in Oryza sativa L. Korean J of Plant Biotechnology 36:130-136   DOI
8 Yoon UH, Lee GS, Kim CK, Lee JS, Hahn JH, Yun DW,, Ji HS, Lee TH, Lee JH, Park SH, Kim GW, Seo MS, Kim YH (2009b) Analysis of germination seed stage expressed sequence tags in Oryza sativa L. Korean J of Plant Biotechnology 36:281-288   DOI
9 Yoon UH, Kim YK, Kim CK, Hahn JH, Kim DH, Lee TH, Lee GS, Park SC, Nahm BH (2010) Current status on expression profiling using rice microarray. Korean J of Plant Biotechnology 37: 144-152   과학기술학회마을   DOI
10 Ouyang S, Zhu W, Hamilton J, Lin H, Campbell M, Childs K, Thibaud-Nissen F, Malek RL, Lee Y, Zheng L, Orvis J, Haas B, Wortman J, Buell CR. (2007) The TIGR Rice Genome Annotation Resource: improvements and new features. Nucleic Acids Res 35(Database issue):D883-7   DOI
11 Paterson AH, Freeling M, Sasaki T. (2008) Grains of knowledge: genomics of model cereals. Genome Res 15:1643-50
12 Qu le Q, Takaiwa F. (2004) Evaluation of tissue specificity and expression strength of rice seed component gene promoters in transgenic rice. Plant Biotechnol J 2:113-25   DOI
13 Qu le Q, Xing YP, Liu WX, Xu XP, Song YR. (2008) Expression pattern and activity of six glutelin gene promoters in transgenic rice. J Exp Bot 59:2417-24   DOI
14 Shewry PR, Napier JA, Tatham AS. (1995) Seed storage proteins: structures and biosynthesis. Plant Cell 7:945-56   DOI
15 Shewry PR, Halford NG. (2002) Cereal seed storage proteins: structures, properties and role in grain utilization. J Exp Bot 53:947-58   DOI
16 Takaiwa F, Yamanouchi U, Yoshihara T, Washida H, Tanabe F, Kato A, Yamada K. (1996) Characterization of common cisregulatory elements responsible for the endosperm-specific expression of members of the rice glutelin multigene family. Plant Mol Biol 30:1207-21   DOI
17 Takaiwa F, Kikuchi S, Oono K. (1986) The structure of rice storage protein glutelin precursor deduced from cDNA. FEBS Lett 206:33-35   DOI
18 Takaiwa F, Kikuchi S, Oono K. (1989) The complete nucleotide sequence of new type cDNA coding for rice storage protein glutelin. Nucleic Acids Res 17:3289   DOI
19 Takaiwa F, Oono K, Wing D, Kato A. (1991) Sequence of three members and expression of a new major subfamily of glutelin genes from rice. Plant Mol Biol 17:875-85   DOI
20 Katsube-Tanaka T, Duldulao JB, Kimura Y, Iida S, Yamaguchi T, Nakano J, Utsumi S. (2004) The two subfamilies of rice glutelin differ in both primary and higher-order structures. Biochim Biophys Acta 1699:95-102   DOI
21 Kawakatsu T, Hirose S, Yasuda H, Takaiwa F. (2010) Reducing rice seed storage protein accumulation leads to changes in nutrient quality and storage organelle formation. Plant Physiol 154:1842-54   DOI
22 Kikuchi S, Satoh K, Nagata T, Kawagashira N, Doi K, Kishimoto N, Yazaki J, Ishikawa M, Yamada H, Ooka H, Hotta I, Kojima K, Namiki T, Ohneda E, Yahagi W, Suzuki K, Li CJ, Ohtsuki K, Shishiki T; Foundation of Advancement of International Science Genome Sequencing & Analysis Group, Otomo Y, Murakami K, Iida Y, Sugano S, Fujimura T, Suzuki Y, Tsunoda Y, Kurosaki T, Kodama T, Masuda H, Kobayashi M, Xie Q, Lu M, Narikawa R, Sugiyama A, Mizuno K, Yokomizo S, Niikura J, Ikeda R, Ishibiki J, Kawamata M, Yoshimura A, Miura J, Kusumegi T, Oka M, Ryu R, Ueda M, Matsubara K; RIKEN, Kawai J, Carninci P, Adachi J, Aizawa K, Arakawa T, Fukuda S, Hara A, Hashizume W, Hayatsu N, Imotani K, Ishii Y, Itoh M, Kagawa I, Kondo S, Konno H, Miyazaki A, Osato N, Ota Y, Saito R, Sasaki D, Sato K, Shibata K, Shinagawa A, Shiraki T, Yoshino M, Hayashizaki Y, Yasunishi A. (2003) Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice. Science 301:376-9   DOI
23 Okita TW, Hwang YS, Hnilo J, Kim WT, Aryan AP, Larson R, Krishnan HB. (1989) Structure and expression of the rice glutelin multigene family. J Biol Chem 264:12573-81
24 Kumar S, Nei M, Dudley J, Tamura K. (2008) MEGA: a biologist- centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform. 9:299-306   DOI
25 Liu WX, Liu HL, Chai ZJ, Xu XP, Song YR, Qu le Q. (2010) Evaluation of seed storage-protein gene 5' untranslated regions in enhancing gene expression in transgenic rice seed. Theor Appl Genet 121:1267-74   DOI
26 Matsuo T, Kumazawa K, Ishii R, Ishihara K, Hirata H. (1995) Science of the rice plant volume two physiology, Nobunkyo press, Tokyo, pp 1063-97
27 Osborn T. (1924) The vegetable proteins. London, FL: Longmans, Green and Co
28 Abe T, Gusti RS, Ono M, Sasahara T. (1996) Variations in glutelin and high molecular weight endosperm proteins among subspecies of rice (Oryza sativa L.) detected by twodimensional gel electrophoresis. Genes Genet Syst 71:63-8   DOI
29 Choi H. (2002) Current status and perspectives in varietal improvement of rice cultivars for high-quality and value-added products. Korean J Crop Sci 47:15-32   과학기술학회마을
30 Bewley DJ, Black M. (1985) Seeds, Physiology of development and germination, Plenum press, New York, pp 1-27
31 Duan M, Sun SS. (2005) Profiling the expression of genes controlling rice grain quality. Plant Mol Biol 59:165-78   DOI
32 International Rice Genome Sequencing Project. (2005) The mapbased sequence of the rice genome. Nature 436:793-800   DOI
33 Jain M, Tyagi AK, Khurana JP. (2008) Genome-wide identification, classification, evolutionary expansion and expression analyses of homeobox genes in rice. FEBS J 275:2845-61   DOI