• KSBB Journal
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• v.22 no.5
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• pp.297-304
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• 2007

For large and rapid screening of high-yielding mutants of lovastatin produced by filamentous fungal cells of Aspergillus terreus, one of the most important stage is to test as large amounts of mutated strains as possible. For this purpose, we intended to develop a miniaturized cultivation method using $7m{\ell}$ culture tube instead of traditional $250m{\ell}$ flask (working volume $50m{\ell}$). For obtaining large amounts of conidiospores to be used as inoculums for miniaturized cultures, 4 components i.e., glucose, sucrose, yeast extract and $KH_2PO_4$ were intensively investigated, which had been observed to show positive effect on enhancement of spore production through Plackett-Burman design experimet. When optimum concentrations of these components that were determined through application of response surface method (RSM) based on central composite design (CCD) were used, maximum spore numbers amounting to $1.9\times10^{10}$ spores/plate were obtained, resulting in approximately 190 fold increase as compared to the commonly used PDA sporulation medium. Using the miniaturized cultures, intensive strain development programs were carried out for screening of lovastatin high-yielding as well as highly reproducible mutants. It was observed that, for maximum production of lovastatin, the producers should be activated through 'PaB' adaptation process during the early solid culture stage. In addition, they should be proliferated in condensed filamentous forms in miniaturized growth cultures, so that optimum amounts of highly active cells could be transferred to the production culture-tube as reproducible inoculums. Under these highly controlled fermentation conditions, compact-pelleted morphology of optimum size (less than 1 mm in diameter) was successfully induced in the miniaturized production cultures, which proved essential for maximal utilization of the producers' physiology leading to significantly enhanced production of lovastatin. As a result of continuous screening in the miniaturized cultures, lovastatin production levels of the 81% of the daughter cells derived from the high-yielding producers turned out to be in the range of 80%$\sim$120% of the lovastatin production level of the parallel flask cultures. These results demonstrate that the miniaturized cultivation method developed in this study is efficient high throughput system for large and rapid screening of highly stable and productive strains.

• Korean Journal of Optics and Photonics
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• v.25 no.6
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• pp.334-339
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• 2014

In this paper, the optical properties of a miniature telecentric camera are studied analytically and experimentally. By means of optical properties, the luminous intensity of retro-reflection of a miniature telecentric optical system is investigated. First, from a simulation the theoretical value of the coefficient of luminous intensity of retro-reflection of a miniature telecentric system is estimated. Second, a miniature telecentric camera device is designed, and the value of the coefficient of luminous intensity of retro-reflection of the actual device is measured through experiment. From simulation and experiment, we found that both the estimated and observed values of the coefficient of luminous intensity of retro-reflection range from 0.002 to $0.03m^2/sr$ (P384200CPH: $0.0042-0.018m^2/sr$, P285200CPH: $0.0045-0.0297m^2/sr$, P321450S: $0.0021-0.00963m^2/sr$) at 1.4 to 4.9 arcminute. The results prove the significance of the mechanisms used to design the miniature telecentric camera, and the experimental method. Accordingly, with these significant results, this study contributes to the development of theory and practice in optical engineering science.

• Reproductive and Developmental Biology
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• v.35 no.3
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• pp.329-334
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• 2011

This study was conducted to establish a freezing method of miniature pig spermatozoa. The semen 더aculated from PWG M-type miniature pig was collected by gloved-hand method. The semen was diluted with same volume extender (m-Modena B). The frozen solution used frozen solution of four different (LEY, TCG, BF-5 and m-Modena+egg yolk) for find optimal frozen solution in miniature pig sperm. The diluted semen for frozen rate assay was added to LEY solution (solution I: 11% lactose+egg yolk; solution II: solution I+glycerol+OEP), and frozen depending on freezing rate by the three different freezing methods (A: until $5^{\circ}C$ for 1 hrs, holding at $-102^{\circ}C$ for 10 min; B: until $5^{\circ}C$ for 2 hrs, holding at $-102^{\circ}C$ for 10 min; C: until $5^{\circ}C$ for 3 hrs, holding at -80 and $-102^{\circ}C$ for 10 min). Semen cooled until $5^{\circ}C$ was added with glycerol 1, 3 and 5%, and take a equilibrium time for 0, 10 and 30min. Frozen-thawed sperm were evaluated for viability, acrosomal status and morphological abnormality. The results of frozen-thawed sperm ability by frozen solution, viability was higher in LEY solution compared to other three different frozen solution. AR pattern of LEY solution were lower than other three different frozen solution. The results of freezing rate, viability was higher in B method compared to other methods (p<0.05). Acrosomal statute was intacted in A and B methods than C method. The experiment for glycerol condition was showed that sperm viability was higher in extender with 1% and 3% glycerol and equilibrium time of 0 min. The acrosome damage was lower in extender with 1% glycerol and equilibrium time of 10 min than other conditions. In conclusion, the optimal conditions for cryopreservation of miniature pig spermatozoa obtained in LEY frozen solution, cooling rate of 1~2 hrs, 1~3% glycerol concentrations and glycerol equilibrium time of 0~10 min.

• Reproductive and Developmental Biology
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• v.31 no.3
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• pp.175-179
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• 2007

The objective of this study was to investigate the effect of thawing temperature on the sperm viability and acrosomal morphology for semen storage of miniature pig by the 0.5ml straw method. In this present study, sperm viability (SYBR-14/PI staining), membrane integrity (Hypoosmotic Swelling Test), acrosome intactness, intensity and capacitation status (chlorotetracycline staining) in frozen miniature pig sperm were evaluated after thawing at 37, 50 and $70^{\circ}C$ for 5, 10 and 45 sec, respectively. Interestingly, the results indicated that sperm thawed at $70^{\circ}C$ for 5 sec significantly (p<0.05) increased sperm viability, but lower the percentage of AR (acrosome reacted spermatozoa) pattern compared to sperm thawed at $37^{\circ}C$ for 45 sec and $50^{\circ}C$ for 10 sec. In terms of thawing condition, high temperature for a short time using the 0.5ml straw was improved cryosurvival of miniature pig semen. Therefore, appropriate thawing method for cryopreservation of miniature pig is required for increasing post-thawing viability.

• Proceedings of the Korean Geotechical Society Conference
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• 2000.03b
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• pp.559-566
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• 2000

본 연구에서는 비등방성 응력조건 하에서 콘 관입속도가 콘 관입시험 결과에 미치는 영향을 연구하기 위하여 유한요소해석 및 Calibration Chamber를 이용한 Miniature Piezocone의 관입시험이 수행되었으며 그 결과를 비교 분석하였다. 비등방성을 고려하기 위하여 Anisotropic Soil Model이 유한요소해석에 이용되었으며 LSU/CALCHAS(Louisiana State University Calibration Chamber System)가 Miniature Piezocone의 관입시험에 이용되었다. 콘 관입속도의 영향이외에도 OCR 및 필터위치의 영향을 고찰하였다.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 1997.04a
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• pp.6-11
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• 1997

In most machining operations, undesirable burr are formed on the edge of the workpiece adjacent to the machined surface. Such burrs are often the cause of various problems during automatic maching process. Therefore, it is very important to know characteristics of burr formation in maching process. This paper describes characteristic of exit burrs generated during miniature drilling process. In particular, the effect of spindle speed, feedrate and drill diameter on burr formaton is investigated. The result showed that exit burr height increased significantly with increasing feedrate.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1995.10a
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• pp.478-481
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• 1995

This paper presents the system demonstrator for an optical fiber sensor system developed as a technological evaluator suitable for generic sensric sensing applications. The new type of fiber-optic sensor employed a diaphragm displacement transforms pressure into optical intensity. Form this sensing technique, we can know the variation of source intensity, the loss of a optical fiber, and the reflectivity of the diaphragm surface. Experimental results are applied to the low-pressure transducer suitable for measuring miniature pressure.

• 한국ITS학회:학술대회논문집
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• v.2007 no.10
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• pp.330-333
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• 2007

• Current Optics and Photonics
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• v.2 no.3
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• pp.221-225
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• 2018

We suggested the use of miniature hollow glass tubes having high ultraviolet (UV) transmission characteristics for the protection of optical-fiber-type UV sensors. We have recently proposed a highly sensitive optical sensor in the UV spectral range, using a fiber Bragg grating (FBG) coated with an azobenzene polymer as the photoresponsive material. In this study, we used UV-transparent miniature glass tubes to protect the etched FBG with the azobenzene polymer coating. This technique will be very useful for protecting various fiber-based UV sensors.

• The Korea Swine Journal
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• v.12 no.4 s.128
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• pp.60-63
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• 1990