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Effect of Thawing Temperature on Sperm Characteristics of Frozen Semen in Miniature Pig  

Choi, Won-Cheol (College of Animal Life Sciences Kangwon National University)
Yang, Mi-Hye (College of Animal Life Sciences Kangwon National University)
Lee, Yong-Seung (College of Animal Life Sciences Kangwon National University)
Cheong, Hee-Tae (School of Veterinary Medicine, Kangwon National University)
Yang, Boo-Keun (College of Animal Life Sciences Kangwon National University)
Lee, Dong-Seok (College of Animal Life Sciences Kangwon National University)
Park, Choon-Keun (College of Animal Life Sciences Kangwon National University)
Publication Information
Reproductive and Developmental Biology / v.31, no.3, 2007 , pp. 175-179 More about this Journal
Abstract
The objective of this study was to investigate the effect of thawing temperature on the sperm viability and acrosomal morphology for semen storage of miniature pig by the 0.5ml straw method. In this present study, sperm viability (SYBR-14/PI staining), membrane integrity (Hypoosmotic Swelling Test), acrosome intactness, intensity and capacitation status (chlorotetracycline staining) in frozen miniature pig sperm were evaluated after thawing at 37, 50 and $70^{\circ}C$ for 5, 10 and 45 sec, respectively. Interestingly, the results indicated that sperm thawed at $70^{\circ}C$ for 5 sec significantly (p<0.05) increased sperm viability, but lower the percentage of AR (acrosome reacted spermatozoa) pattern compared to sperm thawed at $37^{\circ}C$ for 45 sec and $50^{\circ}C$ for 10 sec. In terms of thawing condition, high temperature for a short time using the 0.5ml straw was improved cryosurvival of miniature pig semen. Therefore, appropriate thawing method for cryopreservation of miniature pig is required for increasing post-thawing viability.
Keywords
Miniature pig; Thawing temperature; CTC pattern; Sperm viability; HOST;
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