• Title/Summary/Keyword: Media

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EFFECT OF UNREACTED RESIN MONOMES ON THE ATIVITY OF CARIOGENIC BACTERIA (미반응 레진단량체가 우식유발성 세균의 활성에 미치는 영향)

  • Park, Seung-Kyu;Kim, Hwa-Sook;You, So-Young;Han, Jin-Ju;Kook, Joong-Ki;Lee, Nan-Young;Lee, Sang-Ho;Lee, Chang-Seop
    • Journal of the korean academy of Pediatric Dentistry
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    • v.30 no.4
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    • pp.684-695
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    • 2003
  • The aim of this study was to investigate the effect of composite resin components on proliferation and glucan synthesis by cariogenic bacteria, Streptococcus mutans and Streptococcus sobrinus. Light curing pit and fissure sealant was chosen for evluation. Specimens were eluted in deionized water for 10 minutes, 1, 12, and 24 hours. Extracts of specimens were diluted into 1/2, 1/4, and 1/8 with addition of BHI broth and BHI-YS. Bacteria were cultured in media included eluted components, and measured optical density($A_{600}$). The following results were obtained 1. 1/4 concentration of elutes for 10 minutes significantly inhibited the proliferation of S. mutans, whereas 1/2, 1/8 concentration of elutes stimulated it. Also, exacts, especially 1/2, 1/4 concentration, for 1 hours stimulated it. But exacts for 12, 24 hours had not effects on the proliferation of S. mutans. 2. 1/4 concentration of elutes for 10 minutes inhibited growth of S. sobrinus, whereas extracts for 1, 12, 24 hours had not effects on the proliferation of S. sobrinuss. 3. Extracts from composite resin stimulated total growth of S. mutans more than growth control group, where as inhibited it of S. sobrinus. 4. Extracts from composite resin, especially 1/4 concentration of it for 10 minutes increased the formation of water insoluble glucan of S. mutans. But elutes for 1, 12, 24 hours, and 1/8 concentration of it for 10 minutes inhibited it. 5. Except 1/4 concentration of elutes for 10 minutes, extracts decreased the formation of water insoluble glucan of S. sobrinus. 6. Total amount of formated glucan was 3-fold higher in S. mutans than in S. sobrinus.

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A Study on Infant Weaning Practices Based on Maternal Education and Income Levels (양육인의 교육 및 수입정도에 따른 이유기 식생활관리에 대한 실태조사)

  • Kim, Song-Suk
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.7
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    • pp.1000-1007
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    • 2005
  • The aim of the present study was to examine the relationship of maternal factors such as knowledge, attitude and practice of weaning with infant feeding. The subjects were 103 mothers visiting a public health center in Gumi, Kyungbook who filled out self-administered Questionnaires. First of all, about $90\%$ of the participants recognized the importance of complementary foods and proper weaning practices. The response for the recognition of the importance of infant weaning process showed a significant difference by education levels. Concerning an appropriate time for the introduction of weaning foods, $53\%$ of mothers had commenced weaning at age $4\~6$ months, while $38\%$ had done so at age $6\~8$ months. Approximately $76\%$ of mothers fed their babies without the knowledge of age-related weaning method and type of weaning foods. There were no statistical differences in maternal weaning knowledges between levels of education and house income. Mothers with higher levels of education and family income tended to show high perception scores regarding possibility of food allergies caused by baby foods. A demand for reliable sources and education related to nutritious weaning foods and weaning practices were strong in the group with higher education. Knowledge of weaning method and baby foods were obtained by 59 of the 103 mothers from mass media, 35 from friends caring babies, and 9 obtained advice from health professionals or family. Advice from the heath professionals was not the main influence on their decision to introduce weaning foods. Although commercial baby foods are the most commonly used as first weaning foods, those with higher education groups considered commercial baby food are not nutritionally better than home-maid foods. The current findings suggest to us that to improve weaning process, mothers should be educated on the selection and preparation of nutritious, balanced weaning foods and on good weaning practices. It is advised that supportive health professionals from community public health centers should lead the education of infant feeding practices based on maternal characteristics and on basic food and nutritional knowledge.

Studies on the Growth and nutritional Behaviors of Seedlings Sand Cultured at Different pH Levels (pH 수준별(水準別) 사경묘목(砂耕苗木)의 생장(生長)과 영양동태(營養動態)에 관(關)한 연구(硏究))

  • Son, Won Ha
    • Journal of Korean Society of Forest Science
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    • v.11 no.1
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    • pp.1-24
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    • 1970
  • This experiment was carried out to elucidate the behavior of nutrition absorption and the acid tolerance, on varying soil pH levels, of Pinus thunbergii, Pinus densiflora, Pinus rigida, PInus koraiensis, Larix leptolepis and Abies holophylla which are 6 month old seedlings through examinations of the absorption behavior, the acid and alkali tolerance of N,P,K, Ca and Fe in the sand culture media with artificially controlled pH. 1. The increase rate of dry matter conditioned by the behavior of nutrition absorption in a strong acidic condition (pH3) was found in the following decreasing order of Pinus rigida, Pinus thunbergii. This order, therefore, can also be said to apply to the case of acid tolerance. 2. The increase rate of dry matter in a strong alkaline condition (pH9) was found in the following decreasing order of Pinus thunbergii, Pinus rigida, Pinus densiflora, Pinus koraiensis, Abies hoplohylla, Larix leptolepis. This order, therefore can be said to apply to the case of alkali tolerance. 3. Considering the absorption behavior of K and Ca, it is possible to decide the tolerance of acid from the absorption behavior and it can be said that if the absorption quantity is K>Ca the acid tolerance is strong and vice versa. 4. Considering the ratio of K:Fe and Ca:Fe in this experiment, a strong acid tolerance shows a higher ratio of K/Fe and vice versa. And also a strong acid tolerance shows a lower ratio of Ca/Fe and vice versa. It is therefore possible to decide the acid tolerance from the absorption behavior of K, Ca and Fe. 5. Of the tree species subjected to this experiment, Pinus thunbergii was the strongest in both acid and alkali tolerance. 6. In spite of that in all species the dry weight in the root is smailer than that in the top, the quantity of Fe contained in the root was larger than that in the top.

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EFFECT OF NERVE GROWTH FACTOR GENE INJECTION ON THE NERVE REGENERATION IN RAT LINGUAL NERVE CRUSH-INJURY MODEL (백서 설신경 압박손상모델에서 신경성장인자 유전자 주입이 신경재생에 미치는 영향)

  • Gao, En-Feng;Chung, Hun-Jong;Ahn, Kang-Min;Kim, Soung-Min;Kim, Yun-Hee;Jahng, Jeong-Won;Lee, Jong-Ho
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.28 no.5
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    • pp.375-395
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    • 2006
  • Purpose: Lingual nerve (LN) damage may be caused by either tumor resection or injury such as wisdom tooth extraction, Although autologous nerve graft is sometimes used to repair the damaged nerve, it has the disadvantage of necessity of another operation for nerve harvesting. Moreover, the results of nerve grafting is not satisfactory. The nerve growth factor (NGF) is well-known to play a critical role in peripheral nerve regeneration and its local delivery to the injured nerve has been continuously tried to enhance nerve regeneration. However, its application has limitations like repeated administration due to short half life of 30 minutes and an in vivo delivery model must allow for direct and local delivery. The aim of this study was to construct a well-functioning $rhNGF-{\beta}$ adenovirus for the ultimate development of improved method to promote peripheral nerve regeneration with enhanced and extended secretion of hNGF from the injured nerve by injecting $rhNGF-{\beta}$ gene directly into crush-injured LN in rat model. Materials and Methods: $hNGF-{\beta}$ gene was prepared from fetal brain cDNA library and cloned into E1/E3 deleted adenoviral vector which contains green fluorescence protein (GFP) gene as a reporter. After large scale production and purification of $rhNGF-{\beta}$ adenovirus, transfection efficiency and its expression at various cells (primary cultured Schwann cells, HEK293 cells, Schwann cell lines, NIH3T3 and CRH cells) were evaluated by fluorescent microscopy, RT-PCR, ELISA, immunocytochemistry. Furthermore, the function of rhNGF-beta, which was secreted from various cells infected with $rhNGF-{\beta}$ adenovirus, was evaluated using neuritogenesis of PC-12 cells. For in vivo evaluation of efficacy of $rhNGF-{\beta}$ adenovirus, the LNs of 8-week old rats were exposed and crush-injured with a small hemostat for 10 seconds. After the injury, $rhNGF-{\beta}$ adenovirus($2{\mu}l,\;1.5{\times}10^{11}pfu$) or saline was administered into the crushed site in the experimental (n=24) and the control group (n=24), respectively. Sham operation of another group of rats (n=9) was performed without administration of either saline or adenovirus. The taste recovery and the change of fungiform papilla were studied at 1, 2, 3 and 4 weeks. Each of the 6 animals was tested with different solutions (0.1M NaCl, 0.1M sucrose, 0.01M QHCl, or 0.01M HCl) by two-bottle test paradigm and the number of papilla was counted using SEM picture of tongue dorsum. LN was explored at the same interval as taste study and evaluated electro-physiologically (peak voltage and nerve conduction velocity) and histomorphometrically (axon count, myelin thickness). Results: The recombinant adenovirus vector carrying $rhNGF-{\beta}$ was constructed and confirmed by restriction endonuclease analysis and DNA sequence analysis. GFP expression was observed in 90% of $rhNGF-{\beta}$ adenovirus infected cells compared with uninfected cells. Total mRNA isolated from $rhNGF-{\beta}$ adenovirus infected cells showed strong RT-PCR band, however uninfected or LacZ recombinant adenovirus infected cells did not. NGF quantification by ELISA showed a maximal release of $18865.4{\pm}310.9pg/ml$ NGF at the 4th day and stably continued till 14 days by $rhNGF-{\beta}$ adenovirus infected Schwann cells. PC-12 cells exposed to media with $rhNGF-{\beta}$ adenovirus infected Schwann cell revealed at the same level of neurite-extension as the commercial NGF did. $rhNGF-{\beta}$ adenovirus injected experimental groups in comparison to the control group exhibited different taste preference ratio. Salty, sweet and sour taste preference ratio were significantly different after 2 weeks from the beginning of the experiment, which were similar to the sham group, but not to the control group.

Development and Application of Health Belief Model Based Milk Education Program for Elementary, Middle, and High School Students (건강 신념 모델에 근거한 초.중.고등학교 우유 교육 프로그램 개발 및 적용)

  • Yoo, In-Kyung;Jang, Myung-Hee;Kim, Gyu-Tae;Park, Dong-Ho;Seo, Ji-Young;Park, Sun-Young;Kim, Jung-Hyun
    • Journal of Korean Home Economics Education Association
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    • v.19 no.4
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    • pp.17-36
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    • 2007
  • The purpose of this study was to develop health belief model based various milk education program as print education media and apply to elementary, middle, and high schools. The subjects were 432 students(124 elementary, 122 middle, 186 high school students). We designed one group pretest-posttest study model. The data were obtained from pre and post-study with self-administered questionnaires. Before applying this education program, we evaluated the degree of awareness on milk. Their awareness on milk was very low, 35.5% lower elementary, 32.7% higher elementary, 52.5% middle, and 54.3% high school students were answered they don't know the milk well. After they had implemented milk education program, their recognition on milk had changed that milk is nutritious as supplementary food. And their reasons for drinking milk were also changed that 'they want to eat it' in elementary school students, 'they want to be healthier' in middle school students, and 'they want to be taller' in high school students. Their nutrition knowledge score showed a significant increase(p<0.05). As a results. milk nutrition education has improved nutrition knowledge and recognition on milk in elementary, middle, and high school students. To improve their milk eating behaviors, nutrition education programs will have to be continued.

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Story-based Information Retrieval (스토리 기반의 정보 검색 연구)

  • You, Eun-Soon;Park, Seung-Bo
    • Journal of Intelligence and Information Systems
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    • v.19 no.4
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    • pp.81-96
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    • 2013
  • Video information retrieval has become a very important issue because of the explosive increase in video data from Web content development. Meanwhile, content-based video analysis using visual features has been the main source for video information retrieval and browsing. Content in video can be represented with content-based analysis techniques, which can extract various features from audio-visual data such as frames, shots, colors, texture, or shape. Moreover, similarity between videos can be measured through content-based analysis. However, a movie that is one of typical types of video data is organized by story as well as audio-visual data. This causes a semantic gap between significant information recognized by people and information resulting from content-based analysis, when content-based video analysis using only audio-visual data of low level is applied to information retrieval of movie. The reason for this semantic gap is that the story line for a movie is high level information, with relationships in the content that changes as the movie progresses. Information retrieval related to the story line of a movie cannot be executed by only content-based analysis techniques. A formal model is needed, which can determine relationships among movie contents, or track meaning changes, in order to accurately retrieve the story information. Recently, story-based video analysis techniques have emerged using a social network concept for story information retrieval. These approaches represent a story by using the relationships between characters in a movie, but these approaches have problems. First, they do not express dynamic changes in relationships between characters according to story development. Second, they miss profound information, such as emotions indicating the identities and psychological states of the characters. Emotion is essential to understanding a character's motivation, conflict, and resolution. Third, they do not take account of events and background that contribute to the story. As a result, this paper reviews the importance and weaknesses of previous video analysis methods ranging from content-based approaches to story analysis based on social network. Also, we suggest necessary elements, such as character, background, and events, based on narrative structures introduced in the literature. We extract characters' emotional words from the script of the movie Pretty Woman by using the hierarchical attribute of WordNet, which is an extensive English thesaurus. WordNet offers relationships between words (e.g., synonyms, hypernyms, hyponyms, antonyms). We present a method to visualize the emotional pattern of a character over time. Second, a character's inner nature must be predetermined in order to model a character arc that can depict the character's growth and development. To this end, we analyze the amount of the character's dialogue in the script and track the character's inner nature using social network concepts, such as in-degree (incoming links) and out-degree (outgoing links). Additionally, we propose a method that can track a character's inner nature by tracing indices such as degree, in-degree, and out-degree of the character network in a movie through its progression. Finally, the spatial background where characters meet and where events take place is an important element in the story. We take advantage of the movie script to extracting significant spatial background and suggest a scene map describing spatial arrangements and distances in the movie. Important places where main characters first meet or where they stay during long periods of time can be extracted through this scene map. In view of the aforementioned three elements (character, event, background), we extract a variety of information related to the story and evaluate the performance of the proposed method. We can track story information extracted over time and detect a change in the character's emotion or inner nature, spatial movement, and conflicts and resolutions in the story.

Optimization of Microbial Production of Ethanol form Carbon Monoxide (미생물을 이용한 일산화탄소로부터 에탄올 생산공정 최적화)

  • 강환구;이충렬
    • KSBB Journal
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    • v.17 no.1
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    • pp.73-79
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    • 2002
  • The method to optimize the microbial production of ethanol from CO using Clostridium ljungdahlii was developed. The kinetic parameter study on CO conversion with Clostridium ljungdahlii was carried out and maximum CO conversion rate of 37.14 mmol/L-hr-O.D. and $K_{m}$ / of 0.9516 atm were obtained. It was observed that method of two stage fermentation, which consists of cell growth stage and ethanol production stage, was effective to produce ethanol. When pH was shifted from 5.5 to 4.5 and ammonium solution was supplied to culture media as nitrogen source at ethanol production stage, the concentration of ethanol produced was increased 20 times higher than that without shift. Ethanol production from CO in a fermenter with Clostridium ljungdahlii was optimized and the concentration of ethanol produced was 45 g/L and maximun ethanol productivity was 0.75 g ethanol/L-hr.

Nitrite Accumulation Characteristics According to Hydraulic Retention Time and Aeration Rate in a Biological Aerated Filter (생물여과 반응기에서 수리학적 체류시간 및 폭기량에 따른 아질산 축적 특성)

  • Yoon, Jong Moon;Kim, Dong Jin;Yoo, Ik-Keun
    • Korean Chemical Engineering Research
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    • v.44 no.2
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    • pp.200-206
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    • 2006
  • In a biological aerated filter (BAF) packed with ceramic media (void fraction of BAF=0.32), nitrite accumulation was studied with the variation of hydraulic retention time (HRT) and superficial air velocity. Synthetic ammonium wastewater and petrochemical wastewater were fed at a constant load of $1.6kgNH_4^+-N/m^3{\cdot}d$. Ammonium removal rate was mainly affected by the superficial air velocity in BAF, but nitrite ratio($NO_2-N/NO_x-N$) in the effluent was dependent on both HRT and superficial air velocity. For a fixed HRT of 0.23 hr (corresponding to the empty bed contact time of 0.7 hr) ammonium removal rate was 73/90/92% and nitrite ratio was 0.92/0.82/0.48 at the superficial air velocity of 0.23/0.45/0.56 cm/s, respectively. When HRT is increased to 0.9 hr with superficial air velocity ranging from 0.34 to 0.45 cm/s, the ammonium removal rate was 89% on average. However nitrite ratio decreased significantly down to 0.13. When HRT was further increased to 1.4 hr, ammonium removal rate decreased, thereby resulting in the free ammonia ($NH_3-N$, FA) build-up and nitrite ratio gradually increased (>0.95). Although aeration rate and FA concentration at HRT of 0.23 hr were unfavorable for nitrite accumulation compared with those at HRT of 0.9 hr, nitrite ratio at HRT of 0.23 hr was higher. Taken together, HRT and nitrogen load were found to be critical, in addition to FA concentration and aeration condition, for nitrite accumulation in the BAF tested in the present study.

Characterization of Lecanicillium lecanii Btab0l Isolated with Bioactivities to Tabacco Whitefly (Bemisia tabaci) (담배가루이(Bemisia tabaci) 병원성곰팡이 Lecanicijjium lecanii Btab0l 균주의 특성)

  • Yoon, Yeo-Jun;Yu, Yong-Man;Lee, Min-Ho;Han, Eun-Jung;Hong, Sung-Jun;Ahn, Nan-Hee;Kim, Yong-Ki;Jee, Hyung-Jin;Park, Jong-Ho
    • Korean journal of applied entomology
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    • v.49 no.4
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    • pp.417-422
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    • 2010
  • Cultural characteristics Lecanicillium lecani Btab01 and its insecticidal activity against tobacco whitefly (Bemisia tabaci) were investigated. On potato dextrose agar, tryptic soy agar and SDA+Y media, mycelial growth of L. lecani Btab01 was best at $20{\sim}25^{\circ}C$ and suppressed above $28^{\circ}C$. Both solid culture and liquid culture of L. lecani Btab01 showed high insecticidal activity, 93.9 and 98.3% respectively, against nymph of tobacco whitefly, but there is no significant difference. When culture of L. lecani Btab01 was treated at the concentration of $10^5$, $10^6$, $10^7$ and $10^8$ cfu/ml, their insecticidal activity were 5.8%, 33.8%, 77.3% and 98.5% respectively, and $LT_{50}$ values were 16.1 days, 7.3 days, 5.1 days and 3.5 days respectively. When nymphs were treated by the cultures of L. lecani Btab01 and maintained under saturated condition for zero hour, 24 hours and 168 hours, their control activities were 0%, 20.3% and 100% respectively. Spore germination of L. lecani Btab01 was increased about two times by adding edible oil. When L. lecani Btab01 was treated to control nymph with 0.1% edible oil, it showed high control activity(98.6%) compared to single treatment of L. lecani Btab01 (79.9%).

The Method for Real-time Complex Event Detection of Unstructured Big data (비정형 빅데이터의 실시간 복합 이벤트 탐지를 위한 기법)

  • Lee, Jun Heui;Baek, Sung Ha;Lee, Soon Jo;Bae, Hae Young
    • Spatial Information Research
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    • v.20 no.5
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    • pp.99-109
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    • 2012
  • Recently, due to the growth of social media and spread of smart-phone, the amount of data has considerably increased by full use of SNS (Social Network Service). According to it, the Big Data concept is come up and many researchers are seeking solutions to make the best use of big data. To maximize the creative value of the big data held by many companies, it is required to combine them with existing data. The physical and theoretical storage structures of data sources are so different that a system which can integrate and manage them is needed. In order to process big data, MapReduce is developed as a system which has advantages over processing data fast by distributed processing. However, it is difficult to construct and store a system for all key words. Due to the process of storage and search, it is to some extent difficult to do real-time processing. And it makes extra expenses to process complex event without structure of processing different data. In order to solve this problem, the existing Complex Event Processing System is supposed to be used. When it comes to complex event processing system, it gets data from different sources and combines them with each other to make it possible to do complex event processing that is useful for real-time processing specially in stream data. Nevertheless, unstructured data based on text of SNS and internet articles is managed as text type and there is a need to compare strings every time the query processing should be done. And it results in poor performance. Therefore, we try to make it possible to manage unstructured data and do query process fast in complex event processing system. And we extend the data complex function for giving theoretical schema of string. It is completed by changing the string key word into integer type with filtering which uses keyword set. In addition, by using the Complex Event Processing System and processing stream data at real-time of in-memory, we try to reduce the time of reading the query processing after it is stored in the disk.