• 수산경영론집
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• 제55권1호
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• pp.1-20
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• 2024

Among Japan's agriculture, forestry, fisheries and regional revitalization plans established in June 2018, the key matters related to aquaculture include ① a new perspective on the sponge use system for the development of aquaculture and coastal fisheries, ② a comprehensive strategy for growth and industrialization of aquaculture, and ③ expansion of suitable aquaculture areas. There are four countermeasures including ④ research and development trends. In accordance with these key points, the Japanese government established a comprehensive strategy for aquaculture growth and industrialization by strategically setting aquaculture items in consideration of domestic and international demand, establishing a comprehensive strategy from production to sales and export, and then working in earnest to promote the aquaculture industry. This study analyzes environmental changes surrounding aquaculture in Japan and trends in fish farming and marine products, and summarizes the key points of the June 2018 Agriculture, Forestry, Fisheries and Regional Revitalization Plan to suggest measures that can be utilized in Korea's aquaculture policy.

• 미생물학회지
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• 제51권1호
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• pp.31-38
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• 2015

2012년 2월 남태평양 미크로네시아 축(Chuuk)에서 채집한 두 종의 해양해면 Cinachyrella sp.와 Plakortis sp.의 공생세균의 군집구조를 PCR-DGGE 방법을 사용하여 조사하였다. Cinachyrella sp.와 Plakortis sp. 해면의 total genomic DNA에서 16S rRNA gene-V3 부분을 증폭하여 DGGE를 수행하였으며, 두 종의 해면에서 서로 다른 밴드 패턴이 나타났다. DGGE밴드로부터 16S rRNA gene의 부분 염기서열을 분석한 결과, 알려진 균주의 염기서열들과 87-100%의 유사도를 나타내었다. Cinachyrella sp.의 공생세균 군집구조는 Actinobacteria, Bacteroidetes, Chloroflexi, 그리고 Proteobacteria (Alpha-, Gamma-, Delta-), 6강으로 구성되었다. Plakortis sp.의 공생세균 군집구조는 Actinobacteria, Chloroflexi, Firmicutes, Spirochaetes 그리고 Proteobacteria (Alpha-, Gamma-, Delta-), 7강으로 구성되었다. 두 종의 해면에서 Actinobacteria, Chloroflexi와 Proteobacteria가 공통적으로 존재하였으나 주요 세균군집은 서로 다른 것으로 나타났다. 즉 Cinachyrella sp.의 경우 Proteobacteria가, Plakortis sp.의 경우, Chloroflexi가 주요 세균 군집이었다. 동일지역에서 채집한 서로 다른 두 종의 해면은 각각 다른 공생세균 군집구조를 나타내어 해면 종에 따른 숙주 특이적 분포를 보이는 것으로 나타났다.

• 생약학회지
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• 제28권4호
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• pp.280-285
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• 1997

Pectenotoxin 2 (PTX2), isolated from marine sponges, was examined for its hepatotoxic potential using male ICR mice. PTX2 $(20\;or\;100\;{\mu}g/kg/day,\;ip)$ was administered to mice repeatedly for one or two week. Histopathological examination revealed an increase in granularity in the liver from the mice treated with PTX2. PTX2 did not alter the parameters for hepatotoxicity and nephrotoxicity such as sorbitol dehydrogenase (SDH), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and blood urea nitrogen (BUN). Cytochrome P-450, cytochrome $b_5$, or NADPH cytochrome c reductase was net changed by repeated administration of PTX2. Hepatic microsomal activity of p-nitroanisole O-demethylase, but not aminopyrine N-demethylase, was slightly depressed by PTX2 administerd repeatedly $(100\;{\mu}g/kg/day,\;ip)$ fur 2 weeks. The toxicity of PTX2 $(200\;{\mu}g/kg/day,\;ip)$ was determined in mice pretreated with a metabolic inducer or inhibitor such as phenobarbital, 3-methyl-cholanthrene, $CoCl_2$, or SKF 525-A. Significant alterations in lethality and hepatotoxicity of PTX2 were observed in mice pretreated with a metabolic modulator. The results suggest that liver seems to be the target organ for PTX2 toxicity and also that induction of the PTX2 toxicity may be associated with hepatic drug metabolizing activity.

• Fisheries and Aquatic Sciences
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• 제22권12호
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• pp.30.1-30.11
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• 2019

Marine natural products isolated from the sponge Fascaplysinopsis cf reticulata, in French Polynesia, were investigated as an alternative to antibiotics to control pathogens in aquaculture. The overuse of antibiotics in aquaculture is largely considered to be an environmental pollution, because it supports the transfer of antibiotic resistance genes within the aquatic environment. One environmentally friendly alternative to antibiotics is the use of quorum sensing inhibitors (QSIs). Quorum sensing (QS) is a regulatory mechanism in bacteria which control virulence factors through the secretion of autoinducers (AIs), such as acyl-homoserine lactone (AHL) in gram-negative bacteria. Vibrio harveyi QS is controlled through three parallel pathways: HAI-1, AI-2, and CAI-1. Bioassay-guided purification of F. cf reticulata extract was conducted on two bacterial species, i.e., Tenacibaculum maritimum and V. harveyi for antibiotic and QS inhibition bioactivities. Toxicity bioassay of fractions was also evaluated on the freshwater fish Poecilia reticulata and the marine fish Acanthurus triostegus. Cyclohexanic and dichloromethane fractions of F. cf reticulata exhibited QS inhibition on V. harveyi and antibiotic bioactivities on V. harveyi and T. maritimum, respectively. Palauolide (1) and fascaplysin (2) were purified as major molecules from the cyclohexanic and dichloromethane fractions, respectively. Palauolide inhibited QS of V. harveyi through HAI-1 QS pathway at 50 ㎍ ml^-1 (26 μM), while fascaplysin affected the bacterial growth of V. harveyi (50 ㎍ ml^-1) and T. maritimum (0.25 ㎍). The toxicity of fascaplysin-enriched fraction (FEF) was evaluated and exhibited a toxic effect against fish at 50 ㎍ ml^-1. This study demonstrated for the first time the QSI potential of palauolide (1). Future research may assess the toxicity of both the cyclohexanic fraction of the sponge and palauolide (1) on fish, to confirm their potential as alternative to antibiotics in fish farming.

• 미생물학회지
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• 제54권4호
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• pp.448-452
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• 2018

제주도 성산리 앞 바다 속 해면체로부터 분리한 Bacillus safensis KCTC 12796BP의 유전체를 분석하였다. 그 결과 3,935,874 bp의 환형 염색체와 36,690 bp의 plasmid 염기 서열을 확인하였다. 염색체는 G + C 함량이 41.4%로 75개의 위유 전자를 포함한 3,980개의 코딩 서열을, plasmid는 G + C 함량이 37.3%로 36개의 코딩 서열을 포함하고 있었다. 염색체 코딩 서열 중에는 81개의 tRNA 유전자, 24개 rRNA 유전자와 1개의 tmRNA 유전자가 있었다. 또한 포자 생성에 필요한 30개의 유전자, 포자피를 지령하는 16개의 유전자, 그리고 발아에 필요한 20개의 유전자도 발견되었다. 이외에 협막 다당체 생합성에 필요한 유전자와 편모 생합성 및 주화성에 필요한 유전자, 그리고 염 내성에 필요한 glycine-choline betaine 수송체에 관한 유전자도 존재하였다. 무엇보다도 항알러지활성을 보이는 이차대사산물 seongsanamide의 생합성을 지령하는 비리보좀성 펩타이드 합성효소 유전자를 확인할 수 있었다.

• Biomolecules & Therapeutics
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• 제24권4호
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• pp.347-362
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• 2016

Marine sponges have been considered as a drug treasure house with respect to great potential regarding their secondary metabolites. Most of the studies have been conducted on sponge's derived compounds to examine its pharmacological properties. Such compounds proved to have antibacterial, antiviral, antifungal, antimalarial, antitumor, immunosuppressive, and cardiovascular activity. Although, the mode of action of many compounds by which they interfere with human pathogenesis have not been clear till now, in this review not only the capability of the medicinal substances have been examined in vitro and in vivo against serious pathogenic microbes but, the mode of actions of medicinal compounds were explained with diagrammatic illustrations. This knowledge is one of the basic components to be known especially for transforming medicinal molecules to medicines. Sponges produce a different kind of chemical substances with numerous carbon skeletons, which have been found to be the main component interfering with human pathogenesis at different sites. The fact that different diseases have the capability to fight at different sites inside the body can increase the chances to produce targeted medicines.

• Natural Product Sciences
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• 제10권6호
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• pp.272-276
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• 2004

During the course of our screening for bioactive metabolites from marine sponges, EZZ, the inseparable 1:1 mixture of (8E,13Z,20Z)-strobilinin and (7E,13Z,20Z)-felixinin has been found to deliver significant cytotoxicity against some cancer cell lines. In this study, the antioxidant activity of EZZ was first time evaluated by a series of antioxidant assays. It was found that EZZ was weak in scavenging the stable free radical 1,1-diphenyl-2-picrylhyrazyl (DPPH), but it was comparable to ascorbic acid in scavenging ABTS and superoxide radicals. In addition, EZZ could protect DNA from hydroxyl radical-induced strand cleavage. The findings of the present study suggest that EZZ possess certain antioxidant activity, which might help to prevent occurrence of cancer by alleviating the oxidative stress in cells.

• 한국해양환경ㆍ에너지학회지
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• 제3권2호
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• pp.49-59
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• 2000

장전항 시설물의 최적설계를 위한 항내 파랑분포 수치 시뮬레이션을 수행하였다. 장전항 인근 해역의 바람자료에 기초한 극치동계해석에 의해 추정된 심해 설계파를 수치 시뮬레이션의 외해 경계조건으로 적용하였다. 파랑 시뮬레이션을 위해 Boussinesq 천해파 이론을 사용하였으며, 파의 분산성과 비선형성을 포함하였다. 해안 경계에 대해서는 파랑의 부분적인 반사가 가능토록 유공을 두거나, 파랑 에너지를 모두 흡수하는 해면층을 두어 모사하였다. 방파제 설계 파고를 산정하기 위해서 광역모델에 대한 시뮬레이션을 수행하였으며, 설계된 방파제 및 부두 배치에 대한 항내 정온도 해석을 위하려 상세모델에 대한 시뮬레이션을 수행하였다.

• Animal cells and systems
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• 제8권3호
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• pp.139-143
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• 2004

Two new species of the genus Psammocinia (Dictyoceratida, Irciniidae), P. conulosa n. sp. and P. ulleungensis n. sp., are described from Namhaedo Island and Ulleungdo Island, Korea. Psammocinia conulosa n. sp. seems to be close to both P. amodes Cook and Bergquist, 1998 and P. hawere Cook and Bergquist, 1996 on the basis of the skeletal structure. However, these three species are clearly separated by the following differences: Shape of P. amodes is spatulate and thin, with a broad blade narrowing to a semi-cylindrical stalk; P. hawere forms cups with a shallow excavated bowl, and attached to the substratum by a narrow base. The whole surface of the new species is very finely conulose. Psammocinia ulleungensis n. sp. is similar to P. gageoensis Sim and Lee, 2001 in shape, but this species is easily distinguished from P. gageoensis by the simple skeletal structure.

• Toxicological Research
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• 제13권3호
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• pp.183-186
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• 1997

Acute toxicity of pectenotoxin 2 (PTX2) was examined in mice. Treatment of mice with a toxic dose of PTX2 resulted in clinical signs such as ataxia, cyanosis and an abrupt decrease in body temperature. Histopathological studies revealed that the liver is the major target organ for PTX2. Activities of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and sorbitol dehydrogenase (SDH) were significantly elevated by PTX2 administration. Glucose-6-phosphatase activities were not changed by the treatment. The PTX2 treatment decreased relative liver weight without changing the body weight. The effect of PTX2 on hepatic drug metabolizing enzyme system was determined. An ip dose of PTX2 (200 $\mu$g/kg) induced a significant decrease in the hepatic microsomal protein content. Cytochrome P-450 content, cytochrome b$_5$ content, NADPH cytochrome c reductase, aminopyrine N-demethylase activities, or hepatic glutathione content were not altered by PTX2 treatment.