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http://dx.doi.org/10.1186/s41240-019-0145-0

Potential of fascaplysin and palauolide from Fascaplysinopsis cf reticulata to reduce the risk of bacterial infection in fish farming  

Mai, Tepoerau (EIO, ILM, IFREMER, IRD, UPF)
Toullec, Jordan (EIO, ILM, IFREMER, IRD, UPF)
Wynsberge, Simon Van (EIO, ILM, IFREMER, IRD, UPF)
Besson, Marc (PSL Research University: EPHE-UPVD-CNRS)
Soulet, Stephanie (EIO, ILM, IFREMER, IRD, UPF)
Petek, Sylvain (EIO, ILM, IFREMER, IRD, UPF)
Aliotti, Emmanuelle (EIO, ILM, IFREMER, IRD, UPF)
Ekins, Merrick (Queensland Museum)
Hall, Kathryn (Queensland Museum)
Erpenbeck, Dirk (Department of Earth and Environmental Sciences & GeoBio-Center, Ludwig-Maximilians-Universitat Munchen)
Lecchini, David (PSL Research University: EPHE-UPVD-CNRS)
Beniddir, Mehdi A. (Biocis, Equipe "Pharmacognosie-Chimie des substances naturelles", CNRS, Universite de Paris Sus, Univ. Paris-Saclay)
Saulnier, Denis (EIO, ILM, IFREMER, IRD, UPF)
Debitus, Cecile (EIO, ILM, IFREMER, IRD, UPF)
Publication Information
Fisheries and Aquatic Sciences / v.22, no.12, 2019 , pp. 30.1-30.11 More about this Journal
Abstract
Marine natural products isolated from the sponge Fascaplysinopsis cf reticulata, in French Polynesia, were investigated as an alternative to antibiotics to control pathogens in aquaculture. The overuse of antibiotics in aquaculture is largely considered to be an environmental pollution, because it supports the transfer of antibiotic resistance genes within the aquatic environment. One environmentally friendly alternative to antibiotics is the use of quorum sensing inhibitors (QSIs). Quorum sensing (QS) is a regulatory mechanism in bacteria which control virulence factors through the secretion of autoinducers (AIs), such as acyl-homoserine lactone (AHL) in gram-negative bacteria. Vibrio harveyi QS is controlled through three parallel pathways: HAI-1, AI-2, and CAI-1. Bioassay-guided purification of F. cf reticulata extract was conducted on two bacterial species, i.e., Tenacibaculum maritimum and V. harveyi for antibiotic and QS inhibition bioactivities. Toxicity bioassay of fractions was also evaluated on the freshwater fish Poecilia reticulata and the marine fish Acanthurus triostegus. Cyclohexanic and dichloromethane fractions of F. cf reticulata exhibited QS inhibition on V. harveyi and antibiotic bioactivities on V. harveyi and T. maritimum, respectively. Palauolide (1) and fascaplysin (2) were purified as major molecules from the cyclohexanic and dichloromethane fractions, respectively. Palauolide inhibited QS of V. harveyi through HAI-1 QS pathway at 50 ㎍ ml-1 (26 μM), while fascaplysin affected the bacterial growth of V. harveyi (50 ㎍ ml-1) and T. maritimum (0.25 ㎍). The toxicity of fascaplysin-enriched fraction (FEF) was evaluated and exhibited a toxic effect against fish at 50 ㎍ ml-1. This study demonstrated for the first time the QSI potential of palauolide (1). Future research may assess the toxicity of both the cyclohexanic fraction of the sponge and palauolide (1) on fish, to confirm their potential as alternative to antibiotics in fish farming.
Keywords
Porifera; Marine natural products; Quorum sensing inhibitors; Antibiotic; Fascaplysinopsis cf reticulata;
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