• 약학회지
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• 제54권5호
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• pp.377-386
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• 2010

Single-dimensional (1-D) and two-dimensional (2-D) LC methods were utilized to separate peptides from various sources followed by MS/MS analysis. Two-dimensional ultra-high performance liquid chromatography is a useful tool for proteome analysis, providing a greater peak capacity than 1-D LC. The most popular 2-D LC approach used today for proteomic research combines strong cation exchange and reversed-phase LC. We have evaluated an alternative mode for 2-D LC of peptides using 2-D RP-RP nano UPLC Q-TOF Mass Spectrometry, employing reversed-phase columns in both separation dimensions. As control experiments, we identified 129 proteins in 1-D LC and 322 proteins in 2-D LC from E. coli extract peptides. Furthermore, we applied this method to rat primary hepatocyte and a total of 170 proteins were identified from 1-D LC, and 527 proteins were identified from all 2-D LC system. The in-depth protein profiling established by this 2-D LC MS/MS from rat primary hepatocyte could be a very useful reference for future applications in regards to drug induced liver toxicity.

• 한국환경보건학회지
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• 제36권1호
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• pp.52-60
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• 2010

High performance liquid chromatography (HPLC) and liquid chromatography mass spectrometry (LC/MS) have been widely used to quantify aflatoxins in food, but these methods are expensive, time-consuming, unsuitable for analysis of the routine screening of large sample numbers and require derivatization and high level techniques to perform. The objective of this study is to detect aflatoxins in a large number of foods by a high efficient analytical system of combined enzyme linked immunosorbent assay (ELISA) for screening and LC/MS/MS for confirmation. The samples spiked individually with aflatoxin $B_1$ (0.5 and 1.0 ng/g) and total aflatoxins (10 ng/g) were analyzed by ELISA and LC/MS/ MS, and the recoveries for ELISA and LC/MS/MS were 71.8~119.2% and 70.8~135.3%, respectively. A total of 378 samples (grains, nuts, soybean and fermented soybean foods, pepper and fermented pepper foods) were purchased from the six major cities in Korea and analyzed by ELISA-LC/MS/MS system. Twenty two (5.8%; peanut: 11, pistachio: 2, walnut: 6, almond: 1, pepper powder: 1, pepper paste: 1) out of 378 samples were screened as aflatoxin B1 positive by ELISA, but, 4 (1.1%; peanut: 2, pistachio:1, pepper powder: 1) out of the 22 samples screened were confirmed as aflatoxins positive at levels of 1.02~52.79 ng/g by LC/MS/MS. ELISA-LC/MS/MS system provides a more rapid, accurate and cost-effective method for the detection of aflatoxins in large number of samples.

• 한국안전학회지
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• 제34권5호
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• pp.31-36
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• 2019

This study made a testing device to evaluate the distribution board management system. Power was supplied to the testing device using a loading-back method and the voltage applied to it was 440 V at the same turn ratio. When the human body electric shock current is 30 mA, the breaking time is set to be less than 240 ms while 30~45 mA current is flowing. The test result shows that in the case of the R-phase it was measured to be 5.19 Hz (193 ms). And the S-phase and T-phase were perfectly cut off at 5.39 Hz (186 ms) and 5.71 Hz (175 ms), respectively. When the human body electric shock current is 60mA, the breaking time is set to be less than 120 ms while 45~75 mA current is flowing. The test result shows that the R-phase, S-phase, and T-phase were accurately cut off at 8.39 Hz (11 ms), 8.87Hz (113 ms) and 9.69 Hz (103 ms), respectively. When the human body electric shock current is 90 mA, the breaking time is set to be less than 48 ms while 75 mA current is flowing. The test result shows that the R-phase, S-phase, and T-phase were accurately cut off at 19.8 Hz (50.4 ms), 16.9 Hz (59.2 ms), and 17.9 Hz (56.0 ms), respectively. That is, the developed testing device satisfied all the requirements of the distribution board evaluation criteria, and it becomes available for the performance evaluation of the distribution board management system.

• Food Science and Biotechnology
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• 제14권4호
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• pp.446-449
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• 2005

Two single-magnetron heating systems (SM-HS), each with a helical glass heat exchanger and a cylindrical cavity, were combined to make a two-magnetron-in-series heating system (2MS-HS) in order to increase the heating capacity. A comparison using water showed that the heating performance of the 2MS-HS was increased by two-fold as compared to that of the SM-HS, resulting in energy saving of 7.0% in 2MS-HS. Pasteurization test of 2MS-HS conducted with model food (LB broth contaminated with Bacillus subtilis) showed two-fold higher treatment capacity compared to SM-HS. Relationships between outlet temperature of the processed food, flow rate, and residence time in the 2MS-HS were established for water. Optimum pasteurization capacity was 17 s, $73^{\circ}C$, at flow rate of 280 ml/min. The 2MS-HS could be applied to the small-scale pasteurization of liquid food.

• Journal of Nutrition and Health
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• 제36권10호
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• pp.990-996
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• 2003

This study was conducted to investigate the effects of Forsythia viridissima Lindl. (FVL) on antioxidative defense system and lipid peroxidation of liver in rats fed high-cholesterol diet. Sprague-Dawley male rats weighing 100 $\pm$ 10 g were randomly assigned into five experimental groups fed 0.5％ cholesterol ; HC group which was not supplemented FVL extract, 0.05％ methanol extract diet group (MSI group), 0.1％ methanol extract diet group (MS2 group), 0.025％ ethylacetate-souble fraction diet group (ES1 group) and 0.05％ ethylacetate-souble fraction diet group (ES2 group). Experimental diets were fed ad libitum to the rats for 3 weeks. The hepatic xanthine oxidase (XOD) activity in the MS2 group was decreased to 20％ as compared to HC group. The activities of hepatic superoxide dismutase (SOD) and catalase (CAT) were not significantly different among all the high cholesterol diet groups. The hepatic glutathione peroxidase (GSHpx) activity in MS2, ES2 groups were significantly increased as compared to HC group. The hepatic glutathione S-transferase (GST) activity in the MS2 group was increased to 20％ as compared to HC group. The levels of hepatic TBARS in the MS1, MS2, ES1 and ES2 groups were reduced by 13％, 21％, 13％ and 21％, respectively, as compared with HC group. The contents of lipofuscin in liver tissue was not significantly different among all the experimental groups. The results indicate that FVL extract may reduce oxidative damage by activating antioxidative defense system of liver in rats fed high-cholesterol diets.

• 천문학논총
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• 제13권1호
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• pp.85-98
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• 1998

In this paper we describe MS-TCS, the telescope control system which was developed in Korea Astronomy Observatory. MS-TCS can control an equatorial type telescope equipped with stepping motors and incremental type optical encoders. MS-TCS consists of (1) POINT_TEL which is the program roning in a PC and (2) TCS-196 which is the electroics board to control the telescope. The communication between the PC and TCS-l96 is done through RS-232 or RS-422 serial line. MS-TCS can control the secondary mirror and dome. It also provide network function using TCP/IP for remote control of the telescope. MS-TCS is suitable for controlling medium to small size telescope for research and education.

• 한국통신학회논문지
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• 제31권6A호
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• pp.627-633
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• 2006

MBC(meteor burst communications)는 그 전송로의 많은 장점에도 불구하고 도통율이 수%이하로 낮다는 큰 결점이 있다. 이를 위해 큰 경제적 부담 없이 전송 채널의 도통율을 향상시키는 방법으로서 간단한 구조의 DS(Direct Sequence)시스템을 제안하였다. 제안된 DS 간이 수신방식은 동기신호의 포착이나 유지에 소요되는 시스템을 생략할 수가 있어서 MBC가 가지는 경제성을 만족시킬 수 있으나, 잡음의 영향을 크게 받아 도통율이 떨어지는 단점이 있다. 이를 위해 제안된 DS 시스템에 Transversal Filter회로를 적용하여 SNR을 6dB이상 향상시켰다. 이 방법으로 개선되는 효과는 수신신호가 약한 환경에서 보다 더 큰 것으로 나타나는데 시뮬레이션에 의하면 SNR이 약 6dB 개선되었을 때 평균 버스트 길이가 100ms인 것은 70%로 개선되어 170ms로 되고, 50ms환경에서는 130%개선되어 115ms가 되어 수신신호가 약한 환경에서 도통율이 크게 개선됨을 확인할 수 있었다.

• ETRI Journal
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• 제32권2호
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• pp.255-264
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• 2010

Interference scenarios and methodologies between a terrestrial mobile service (MS) system and mobile-satellite service (MSS) system in a co-channel environment are established. Taking into account a practical deployment situation for both systems, we perform computational simulation of interference in terms of carrier-to-interference ratio (C/I) and interference-to-noise ratio (I/N) to evaluate the cofrequency interference from an MS system into an MSS system, and from an MSS system into an MS system, respectively. The methodology and results can be used as a guide when planning the deployment of MSS and MS systems with no unacceptable interference impact between them.

• 대한한의학방제학회지
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• 제32권3호
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• pp.223-233
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• 2024

Objectives : Natural products containing bioactive compounds with high antioxidant activity are potentially important sources that can contribute to the improvement of various diseases. Therefore, the aim of this study was to investigate phenolic compounds of Cinnamomum cassia (C. cassia) ethanolic extract (CCEE). And then we evaluated the antioxidant effect. Methods : We used liquid chromatography with tandem mass spectrometry (LC-MS/MS) to identify the compounds in CCEE. LC-MS/MS was performed in positive ion mode using Shimadzu, Nexera HPLC system and IDA TOF mass system. Solvent A was distilled water and solvent B was acetonitrile as mobile phase. The analysis was performed at a flow rate of 0.5 ml/min, column temperature of 35 ℃ and wavelength of 284 nm. The antioxidant effect of CCEE was analyzed using DPPH (2,2-diphenyl-2-picrylhydrazyl free radical) and ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)). In addition, total phenolics and total flavonoids contents were measured to determine antioxidant effects. Results : Analysis using LC-MS/MS identified four compounds: Coumarin, Trans-cinnamaldehyde, Trans-cinnamic acid, and 2-Methoxycinnamaldehyde. Free radicals decreased in a concentration-dependent manner starting from 10 ㎍/ml of CCEE, and decreased to a level similar to Ascorbic acid (AA) from a concentration of 60 ㎍/ml onwards. Conclusions : Based on the findings, CCEE exhibits strong antioxidant activity as evidenced by the presence of Coumarin, Trans-cinnamaldehyde, Trans-cinnamic acid, and 2-Methoxycinnamaldehyde. Consequently, this study suggests that CCEE can serve as an important source of natural antioxidants and can be efficiently used in the management of oxidative stress diseases.

• Communications for Statistical Applications and Methods
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• 제29권2호
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• pp.151-160
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• 2022

The total system is composed of the main system (MS) and the failure detection equipment (FDE) which detects failures of MS. The analysis of system reliability is performed when the failure of FDE is possible. Several repair policies are considered to determine the order of repair of failed systems, which are sequential repair (SQ), priority repair (PR), independent repair (ID), and simultaneous repair (SM). The states of MS-FDE systems are represented by Markov models according to repair policies and the main purpose of this paper is to derive the system availabilities of the Markov models. Analytical solutions of the stationary equations are derived for the Markov models and the system availabilities are immediately determined using the stationary solutions. A simple illustrative example is discussed for the comparison of availability values of the repair policies considered in this paper.