• Archives of Pharmacal Research
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• 제28권9호
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• pp.1086-1091
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• 2005

An analytical method was developed for evaluating the cannabidiol (CBO), cannabinol (CBN), ${\Delta}^9-tetrahydrocannabinol$ $({\Delta}^9-THC)$ level in human hair using gas chromatography-mass spectrometry (GC-MS). Hair samples (50mg) were washed with isopropyl alcohol and cut into small fragments (< 1mm). After adding a deuterated internal standard, the hair samples were incubated in 1.0M NaOH for 10 min at $95^{\circ}C$. The analytes from the resulting hydrolyzed samples were extracted using a mixture of n-hexane-ethyl acetate (75:25, v/v). The extracts were then evaporated, derivatized, and injected into the GC-MS. The recovery ranges of CBD, CBN, and ${\Delta}^9-THC$ at three concentration levels were $37.9-94.5\%$ with good correlation coefficients $(r^2>0.9989)$. The intra-day precision and accuracy ranged from $-9.4\%\;to\;17.7\%$, and the inter-day precision and accuracy ranged from $-15.5\%\;to\;14.5\%$, respectively. The limits of detection (LOD) for CBD, CBN, and ${\Delta}^9-THC$ were 0.005, 0.002, and 0.006 ng/mg, respectively. The applicability of this method of analyzing the hair samples from cannabis abusers was demonstrated.

• 한국식품위생안전성학회지
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• 제34권6호
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• pp.519-525
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• 2019

건강기능식품의 경우 단성분보다는 복합제품 개발이 우선시되고, 12가지 기본제형이외에도 일반식품형태의 제품으로 범위가 확대되고 있다. 이러한 제품에서 기능성(지표)성분을 효율적으로 분석할 수 있는 방법에 대한 연구가 필요한 실정이다. 복합제품 등에서 정성·정량 가능한 코엔자임Q10을 효과적으로 분석할 수 방법을 확립하기 위하여 본 연구를 수행하였다. 액체크로마토그라프-질량분석기(LC-MS/MS)를 이용하여 건강기능식품 중 코엔자임Q10의 정성·정량시험법을 확립하였다. 확립된 시험법에 대해 특이성, 검출한계, 정량한계, 정확도, 정밀도에 대한 검증을 통하여 유효성을 확인하였다. 또한 시중 유통검체 14건을 확보하여 시험의 적용가능성도 검토하였다. 직선성에서는 R²>0.999 이상임을 확인하였고, 검출한계 및 정량한계는 각각 26.0 및 78.9 ㎍/L이었다. 또한 평균 회수율은 98.6-107.0%로 나타났으며, 반복정밀도는 상대표준편차 4.0%이하, 실험실간 재현성은 2.4%로 나타나 정확성, 재현성이 우수하였으며 이는 AOAC 가이드라인에서 제시한 기준에 모두 적합한 수준이었다. 시중 유통 제품에 대하여 실험한 결과 84.0-118.7%로 모두 적합하였다. 따라서 본 연구에서 개발된 분석법은 건강기능식품 중 코엔자임Q10의 정성·정량분석에 이용될 수 있으며, 우수한 품질의 건강기능식품유통에 기여할 것으로 판단된다.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.285.2-286
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• 2003

Analysis of illicit methamphetamine samples seized in Korea is discussed. The samples are extracted with the small portion of ethyl acetate under neutral conditions and the extracts are analyzed by GC-MS. Several impuritiy peaks are found in each chromatrogram. Eight compunds (1,2-Dimethyl-3-phenylaziridine, amphetamine oxime, ephedrine, N-formylmethamphetamine, N-acetylmethamphetamine, acetylephedrine, 3,4-dimethyl-5-phenyl-2-oxazolidone, methamphetamine dimer) are identified impurities in illicit methamphetamine and the identity of the impurity is conformed synthesis. (omitted)

• 한국산업보건학회지
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• 제17권3호
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• pp.192-202
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• 2007

Objectives: The objectives of this research are to examine the missions, organization, and programs of the environmental, health and safety(EHS) offices of the major research universities in the U.S.A., particularly the case of Massachusetts Institute of Technology(MIT) EHS Office, and to suggest ways to address the EHS issues of the universities and research institutes in South Korea. Methods: The top 30 research universities in the U.S.A. were selected by the total amounts of research funds they annually received. The web sites of the 30 universities were searched to identify the names of the departments that address environment, health, and/or safety related issues, the missions of those departments, the major programs of those departments, and the number of staff in those departments. Also, a case study was conducted for the Environment Health and Safety Management System and the Environment, Health and Safety Office of the MIT, using literature and web searches and a meeting with the Director of the MIT Environment, Health and Safety Office. Results: All the top 30 research universities in the U.S.A. had designated departments that dealt with EHS issues. Most of them were by the name of or similar to environmental, health and safety. The mission statements of those departments were mostly about improving the safety and health of their community members, improving the environment, and complying to EHS regulations. Most of those departments had an environmental management program, industrial hygiene program, radiation protection program, safety program, and biosafety program as their major programs. The components of the environment, health and safety management system(EHS-MS) of the MIT were examined in detail. In contrast, not many universities in South Korea had designated departments that dealt specifically with EHS issues. Also, the number of fulltime staff for EHS was only 1-2 in most of the Korean universities and their work duties included only general safety, while neglecting other health/environment related issues. Conclusions: Well organized and functioning environmental, health and safety offices were present in all of the top 30 research universities in the U.S.A., whereas similar organizations of the universities in South Korea were virtually non-existent and/or had very limited EHS activities. Therefore, in order to reduce potential risks of accidents and health problems in the Korean universities and research institutions, well established and functioning EHS-MSs and EHS offices are warranted. The case of the EHS-MS and EHS Office in the MIT demonstrate a successful case to follow.

• 한국대기환경학회지
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• 제30권5호
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• pp.477-491
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• 2014

This study was carried out to evaluate the concentration variations of volatile organic compounds (VOCs) in the office environments located in a large urban area with respect to seasonality, smoking status, types of ventilation and heating. Indoor air sampling was undertaken in 37 and 30 offices in Daegu city during summer and winter, respectively. The VOC samples were collected using adsorbent tubes, and were determined by thermal desorption coupled with GC/MS analysis. The analytical method was validated for repeatability, method detection limits (MDL), and duplication precision. A total of 34 VOCs of environmental concern were determined, including 15 aromatics and 19 halogenated hydrocarbons. Average concentrations of BTEX appeared to 1.91 ppb, 22.98 ppb, 3.44 ppb, and 3.70 ppb, respectively. These values were relatively higher levels than those measured at homes and outdoor roadsides reported by other researches. In general, the concentrations of VOCs were higher in winter than summer, in smoking offices than non-smoking offices, in forced ventilation type than natural ventilation type, and in combustion heating than non-combustion heating offices. However, such differences were not always significant at a level of 0.05 by statistical tests (t-test and/or Mann-Whitney test) with some exceptions for BTEX and styrene. This study demonstrated that smoking status, ventilation type and presence of combustion sources indoors could be important factors on the elevated concentrations of some VOCs in the office environment.

• 분석과학
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• 제33권2호
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• pp.98-107
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• 2020

Methamphetamine (MA) is currently the most abused illicit drug in Korea. MA is produced by chemical synthesis, and the final target drug that is produced contains small amounts of the precursor chemicals, intermediates, and by-products. To identify and quantify these trace compounds in MA seizures, a practical and feasible approach for conducting chromatographic fingerprinting with a suite of traditional chemometric methods and recently introduced machine learning approaches was examined. This was achieved using gas chromatography (GC) coupled with a flame ionization detector (FID) and mass spectrometry (MS). Following appropriate examination of all the peaks in 71 samples, 166 impurities were selected as the characteristic components. Unsupervised (principal component analysis (PCA), hierarchical cluster analysis (HCA), and K-means clustering) and supervised (partial least squares-discriminant analysis (PLS-DA), orthogonal partial least squares-discriminant analysis (OPLS-DA), support vector machines (SVM), and deep neural network (DNN) with Keras) chemometric techniques were employed for classifying the 71 MA seizures. The results of the PCA, HCA, K-means clustering, PLS-DA, OPLS-DA, SVM, and DNN methods for quality evaluation were in good agreement. However, the tested MA seizures possessed distinct features, such as chirality, cutting agents, and boiling points. The study indicated that the established qualitative and semi-quantitative methods will be practical and useful analytical tools for characterizing trace compounds in illicit MA seizures. Moreover, they will provide a statistical basis for identifying the synthesis route, sources of supply, trafficking routes, and connections between seizures, which will support drug law enforcement agencies in their effort to eliminate organized MA crime.

• 분석과학
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• 제31권4호
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• pp.161-170
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• 2018

메스암페타민(methamphetamine, MA)과 암페타민(amphetamine, AP)은 전세계적으로 널리 퍼진합성 흥분제로 보건 및 사회 문제, 경제적 비용 발생 등의 다양한 문제를 일으킨다. 또한 이러한 마약은 중독 및 남용 가능성이 매우 높기 때문에 확산 방지를 위한 방안의 하나로 복용자의 생체시료에서 이들 마약을 검출하기 위한 분석법 개발이 필요하다. 따라서 본 연구에서는 액체크로마토그래피-질량분석법(liquid chromatography-tandem mass spectrometry, LC-MS/MS)를 이용하여 소변에서 MA와 4-하이드록시메스암페타민(4-hydroxymethamphetamine, 4HMA), AP, 4-하이드록시암페타민(4-hydroxyamphetamine, 4HA)을 동시에 분석할 수 있는 분석법을 개발하였고 유효성 평가를 수행하였다. 전처리 시간 단축을 위해 50000 g에서 3 min 동안 초고속원심분리 후 시료 희석 주입법을 이용하여 LC-MS/MS에 주입하였다. 분리관은 역상 C18 컬럼을 사용하였고, 정량분석을 위해 MRM (multiple reaction monitoring) 모드를 적용하였다. 가중계수 $1/x^2$에서 정량 범위는 MA는 10-2500 ng/mL, AP는 1.0-800 ng/mL, 4HA와 4HMA는 2.0-200 ng/mL로 설정하였고, 검량선의 직선성은 결정계수($r^2$)를 구하여 평가하였다. 초고속원심분리법의 효율성을 확인하기 위해 전처리 과정으로 시린지 필터(membrane-filtration)를 적용한 결과와 비교하였고, 그 결과 분석물질에 따라 6-15 % 성능이 우수한 것으로 나타났다. 일내(intra-day)와 일간(inter-day) 정밀도는 6.6 % 미만이었고, 정확도는 -14.9-11.3 % 였다. 최저정량한계(LLOQ)는 2.0 ng/mL (4HA 및 4HMA), 1.0 ng/mL (AP), 10 ng/mL (MA)로 확인되었다. 선택성, 검출한계, 희석무결성, 기질효과, 효율성, 안정성을 평가한 결과 만족스러운 측정값을 얻었다. 또한 개발된 분석법을 마약 복용자의 소변에 적용하여 분석법의 유용성을 확인하였다.

• 분석과학
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• 제34권6호
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• pp.241-250
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• 2021

검량선 작성은 기기분석을 통해 생체시료에서 분석물질의 농도를 측정하는 정량분석법 개발과 측정값의 정확도 향상에 있어서 필수적인 요소이다. 본 연구에서는 R 기반 통계분석기법을 이용하여 개별 분석물질 정량에 적합한 가중계수와 회귀모델 선정하기 위한 단계별 선택 기준을 적용하여 분석 프로그램을 설정하였다. 국내에서 남용빈도가 가장 높은 필로폰과 대마 복용여부 확인을 위해 액체크로마토그래피-질량분석법(LC-MS/MS)이 적용되었으며, 분석물질로 마약의 복용 여부를 확인에 일반적으로 사용되는 대상 마약의 모체와 대사체를 소변 시료에서 분석하였다. 검량선 작성에 있어서 가중계수 적용여부는 원본데이터의 이분산성 검정을 통해 확인하였고, 가중계수가 필요하다고 판단된 경우 분산분석을 통해 적정 가중계수를 선정하였다. 다음으로 편분산분석을 이용하여 회귀모델에 적합한 차수를 결정하였다. 분석물질인 메트암페타민, 암페타민, 대마 대사체에 대해 R 기반 프로그램에 적용한 결과, 단계별 결과 및 최종 모델식을 직관적으로 이해하기 쉽고 신속하게 얻을 수 있었다. 이러한 결과는 문서 파일로 저장이 가능하여 보관의 편의성을 제고하였으며, 본 연구를 통해 제작된 R 기반 프로그램을 활용하여 검량선 작성을 필요로 하는 다양한 약물분석 분야에 확대 적용할 수 있을 것으로 판단된다.

• 약학회지
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• 제55권4호
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• pp.289-294
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• 2011

Illegal addition of steroids into cosmetics, ointments or drugs have been increased and their careless usage induced detrimental effect on health. We developed simultaneous analytical method using TLC, HPLC and LC/MS for the identification of 40 corticosteroids. 34 corticosteroids were well separated in HPLC with isocratic mode and remaining 6 drugs were also separated with gradient mode. All of the 40 corticosteroids were detected in negative mode in LC/MS. Halcinonide, prednisolone, triamcinolone acetonide and methylprednisolone hemisuccinate were detected in real samples.

• 한국수학교육학회지시리즈D:수학교육연구
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• 제12권3호
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• pp.193-200
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• 2008

In this study, an innovative computer support has been suggested to improve differential perception of the students. Research has been conducted on a calculus class which has 35 students. A semi-structured interview has been reported in the study. By this interview, it was tried to make differential concept more understandable by using Micro Soft Excel component of the well-known MS Office software. By this aim, students have been asked to integrate a simple function by using MS Excel. At the end of the study, it was observed that differential concept made more sense in students' minds than previous.