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http://dx.doi.org/10.13103/JFHS.2019.34.6.519

Analytical Method Development for Determination of Coenzyme Q10 by LC-MS/MS in Related Health Functional Foods  

Lee, Jin Hee (Imported Food Analysis Division, Gyeongin Regional Office of Food and Drug Safety)
Oh, Mihyune (Imported Food Analysis Division, Gyeongin Regional Office of Food and Drug Safety)
Publication Information
Journal of Food Hygiene and Safety / v.34, no.6, 2019 , pp. 519-525 More about this Journal
Abstract
The Ministry of Food and Drug Safety (MFDS) is amending its test methods for health functional foods (dietary food supplements) to establish regulatory standards and specifications in Korea. In this regard, we are continuing our research on analytical method development for the items listed in the Korean Health Functional Food Codex. In this study, we have developed a sensitive and selective test method that could simultaneously separate and determine coenzyme Q10 based on liquid chromatographic-tandem mass spectrometry (LC-MS/MS). Calibration curves showed linearity with a correlation coefficient (R2) of > 0.999 and the limits of detection (LODs) and limits of quantitation (LOQs) were in the range of 26.0 ㎍/L and 78.9 ㎍/L, respectively. The recovery results ranged between 98.6-107.0% at 3 different concentration levels with relative standard deviations (RSDs) less than 5%. The proposed analytical method was characterized with high resolution of the coenzyme Q10 and the assay was fully validated as well.
Keywords
Coenzyme Q10; Analytical method; LC-MS/MS; Health functional food;
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