• BMB Reports
• /
• v.43 no.9
• /
• pp.635-641
• /
• 2010

Piwi proteins and Piwi-interacting RNAs (piRNAs) have been implicated in transposon control in germline from Drosophila to mammals. To examine the profile of small RNA expression in human cancer cells and explore difference in small RNA transcriptome, small RNA libraries prepared from wildtype, HILI overexpressed and HILI knockdowned Hela cells were sequenced using Solexa technology. piRNAs and other repeat-associated small RNAs were observed in Hela cells. By using in situ hybridization, piR-49322 was localized in the nucleolus and around the periphery of nuclear membrane in Hela cells. Following the overexpression of HILI, the retrotransposon elements LINE1 was significantly repressed, while LINE1-associated small RNAs decreased in abundance. The present study demonstrated that HILI along with piRNAs plays a role in LINE1 suppression in Hela cancer cell line.

• Journal of Power Electronics
• /
• v.18 no.3
• /
• pp.931-943
• /
• 2018

Wireless power transfer (WPT) technology has been the focus of a lot of research due to its safety and convenience. The Z-source inverter (ZSI) was introduced into WPT systems to realize improved system performance. The ZSI regulates the dc-rail voltage in WPT systems without front-end converters and makes the inverter bridge immune to shoot-through states. However, when the WPT system is combined with a ZSI, the system parameters must be configured to prevent the ZSI from entering an "accidental shoot-through" (AST) state. This state can increase the THD and decrease system power and efficiency. This paper presents a mathematical analysis for the characteristics of a WPT system and a ZSI while addressing the causes of the AST state. To deal with this issue, the impact of the system parameters on the output are analyzed under two control algorithms and the primary compensation capacitance range is derived in detail. To validate the analysis, both simulations and experiments are carried out and the obtained results are presented.

• Herbal Formula Science
• /
• v.17 no.2
• /
• pp.65-72
• /
• 2009

Objectives : Yukmijihwang-tang (YJT) is known as a tonifying formula for reinforcement of yin deficiency conditions. The present study was carried out to investigate the potential acute toxicity of YJT in ICR male and female mice. Methods : We investigated the acute toxicity about boiling water-extracted YJT. The test article was orally administered once by gavage to 20 male and 20 female mice at dose levels of 0 (control group), 1250, 2500 and 5000 mg/kg body weight. Mortalities, clinical findings, autopsy and body weight changes were monitored daily for the 14 days following the administration according to the Regulation of Korean Food and Drug Administration. Results : We observed survival rates, general toxicity, change of body weight, and autopsy. Single oral administration of YJT with different dosages, no animals died of the test drug. Autopsy of animal revealed no abnormal gross finding. Therefore, $LD_{50}$ value of YJT for ICR mice was more than 5000 mg/kg on oral route. Conclusions : These results suggest that no toxic dose level of YJT in mice is considered to be more than 5000 mg/kg. Consequently, it was concluded that YJT have no effect on acute toxicity and side effect in ICR mice.

• Journal of Korean Society on Water Environment
• /
• v.31 no.6
• /
• pp.637-643
• /
• 2015

This study has been carried out to provide the basic data obtained from the evaluation of the variation of water quality around the Chudong intake tower in Daechung resevoir, which may be able to help the establishment of a pure water supply policy. Five sites around the Chudong intake tower were selected, and the average data for recent ten years(from year 2004 to year 2013) were analysed. The average water quality around Chudong intake tower are as followed; pH 7.5, DO 8.7 mg/L, BOD 1.0 mg/L, COD 3.0 mg/L, SS 3.0 mg/L, TN 1.495 mg/L, TP 0.017 mg/L, and Chlorophyll-a 6.5 mg/m³ were matched the good class by comparing with the living environment standard of reservoir. COD values of higher than 3.0 mg/L after July were likely due to non-point pollutants and algae outbreak during rainy summer season. Total phosphorus rose sharply in the summer season, and then algae watch was issued consistently for average 40 days. Total nitrogen to total phosphorus ratio was average 90, and it is important to control the inflow phosphrous from small stream for proper management to block an algae growth according to eutrophication. It was recommended to operate the algae removing boats around intake tower from July to October, and was required advanced water treatment processes to remove NBD COD and bad odor and taste due to algae growth.

• Molecules and Cells
• /
• v.40 no.10
• /
• pp.761-772
• /
• 2017

Glioblastoma is the most frequent and most aggressive brain tumor in adults. Solute carrier family 8 member 2 (SLC8A2) is only expressed in normal brain, but not present in other human normal tissues or in gliomas. Therefore, we hypothesized that SLC8A2 might be a glioma tumor suppressor gene and detected the role of SLC8A2 in glioblastoma and explored the underlying molecular mechanism. The glioblastoma U87MG cells stably transfected with the lentivirus plasmid containg SLC8A2 (U87MG-SLC8A2) and negative control (U87MG-NC) were constructed. In the present study, we found that the tumorigenicity of U87MG in nude mice was totally inhibited by SLC8A2. Overexpression of SLC8A2 had no effect on cell proliferation or cell cycle, but impaired the invasion and migration of U87MG cells, most likely through inactivating the extracellular signal-related kinases (ERK)1/2 signaling pathway, inhibiting the nuclear translocation and DNA binding activity of nuclear factor kappa B ($NF-{\kappa}B$), reducing the level of matrix metalloproteinases (MMPs) and urokinase-type plasminogen activator (uPA)-its receptor (uPAR) system (ERK1/2-$NF-{\kappa}B$-MMPs/uPA-uPAR), and altering the protein levels of epithelial to mesenchymal transitions (EMT)-associated proteins E-cardherin, vimentin and Snail. In addition, SLC8A2 inhibited the angiogenesis of U87MG cells, probably through combined inhibition of endothelium-dependent and endothelium-nondependent angiogenesis (vascular mimicry pattern). Totally, SLC8A2 serves as a tumor suppressor gene and inhibits invasion, angiogenesis and growth of glioblastoma.

• Biomolecules & Therapeutics
• /
• v.15 no.3
• /
• pp.175-181
• /
• 2007

Zizyphi Spinosi Semen (ZSS), a traditional Chinese folk medicine, has been used for treatment of insomnia and anxiety. This experiment was performed to investigate the anxiolytic-like effect of methanol extract of ZSS (MEZSS) in mice by using the experimental paradigms of anxiety and compared with that of a known anxiolytic, diazepam. In the elevated plus-maze test, it showed that MEZSS (100 mg/kg, p.o.) and diazepam (2.0 mg/kg, p.o.) increased the percentage of time spent on the open arms and the number of open arms entries. MEZSS (50, 100 and 200 mg/kg, p.o.) and diazepam (0.5 mg/kg, p.o.) significantly increased the number of head dips compared with that of control group in the hole-board test. However, MEZSS has no effect on decreasing the locomotor activity, while diazepam (2.0 mg/kg, p.o.) significantly inhibited locomotor activity. MEZSS did not decrease the strength force in the grip strength test, either. In addition, GABAergic involvements were also investigated to understand the possible mechanisms. $GABA_{A}$ receptors subunits and glutamic acid decarboxylase (GAD) were not over expressed, compared with that of the saline group. We also found that MEZSS did not increase chloride influx in cultured cerebellar granule cells. It is concluded that MEZSS might have anxiolytic-like effects, but these effects might not be mediated by GABAergic transmission.

• Korean Journal of Organic Agriculture
• /
• v.19 no.1
• /
• pp.83-92
• /
• 2011

This study was established to investigate the effects of Ca application on fruit mineral nutrients, quality, and browning of 'Fuyu' sweet persimmon (Diospyros kaki). Ca foliar application at 40ml/20L/tree had a greater fruit Ca concentration than did control, Ca fertigation (40ml/20L/tree), and Ca foliar application (40ml/20L/tree) coupled with IBA fertigation (40ml/20L/tree). Fruit mineral nutrient concentrations for Mg, B, and Mn were similar or lower in the foliar treatment compared to other treatments. Fruit color was not affected. Ca treatment, whether foliar applied or fertigated, was effective in maintaining fruit firmness and in decreasing the browning symptom in fruit after MA storage at $0^{\circ}C$ for 60 and 100 days. Although Ca foliar application + IBA fertigation treatment improved fruit firmness, the positive effect on the browning and decay occurrences in fruit was not shown.

• Journal of Microbiology
• /
• v.45 no.1
• /
• pp.41-47
• /
• 2007

The Hantaan virus (HTNV) is an enveloped virus that is capable of inducing low pH-dependent cell fusion. We molecularly cloned the viral glycoprotein (GP) and nucleocapsid (NP) cDNA of HTNV and expressed them in Vero E6 cells under the control of a CMV promoter. The viral gene expression was assessed using an indirect immunofluorescence assay and immunoprecipitation. The transfected Vero E6 cells expressing GPs, but not those expressing NP, fused and formed a syncytium following exposure to a low pH. Monoclonal antibodies (MAbs) against envelope GPs inhibited cell fusion, whereas MAbs against NP did not. We also investigated the N-linked glycosylation of HTNV GPs and its role in cell fusion. The envelope GPs of HTNV are modified by N-linked glycosylation at five sites: four sites on G1 (N134, N235, N347, and N399) and one site on G2 (N928). Site-directed mutagenesis was used to construct eight GP gene mutants, including five single N-glycosylation site mutants and three double-site mutants, which were then expressed in Vero E6 cells. The oligosaccharide chain on residue N928 of G2 was found to be crucial for cell fusion after exposure to a low pH. These results suggest that G2 is likely to be the fusion protein of HTNV.

• Proceedings of the Korean Institute of Surface Engineering Conference
• /
• 2018.06a
• /
• pp.72-72
• /
• 2018

A dynamic fatigue characteristic of dental implant system has been evaluated with applying single axial compressive shear loading based on the ISO 14801 standard. For the advanced dynamic fatigue test, multi-directional force and motion needed to be accompanied for more information of mechanical properties as based on mastication in oral environment. In this study, we have prepared loading and motion protocol for the multi-directional fatigue test of dental implant system with single (Apical/Occlusal; AO), and additional mastication motion (Lingual/Facial; LF, Mesial/Distal; MD). As following the prepared protocol (with modification of ISO 14801), fatigue test was conducted to verify the worst case results for the development of highly stabilized dental implant system. Mechanical testing was performed using an universal testing machine (MTS Bionix 858, MN, USA) for static compression and single directional loading fatigue, while the multi-directional loading was performed with joint simulator (ADL-Force 5, MA, USA) under load control. Basically, all mechanical test was performed according to the ISO 14801:2016 standard. Static compression test was performed to identify the maximum fracture force with loading speed of 1.0 mm/min. A dynamic fatigue test was performed with 40 % value of maximum fracture force and 5 Hz loading frequency. A single directional fatigue test was performed with only apical/occlusal (AO) force application, while multi directional fatigue tests were applied $2^{\circ}$ of facial/lingual (FL) or mesial/distal (MD) movement. Fatigue failure cycles were entirely different between applying single-directional loading and multi-directional loading. As a comparison of these loading factor, the failure cycle was around 5 times lower than single-directional loading while applied multi-directional loading. Also, the displacement change with accumulated multi-directional fatigue cycles was higher than that of single directional cycles.

• Restorative Dentistry and Endodontics
• /
• v.23 no.2
• /
• pp.690-700
• /
• 1998

Rubber-toughened particles which are used in the field of chemical engineering are used to increase the fracture toughness of thermoset resin. The application of Core-Shell particles, one of rubber-toughened particles, as a filler for dental composite or restoration have not been examined. The purpose of this study was to evaluate possible use of Core-Shell particles for dental composite, and the hypothesis was that additional toughening mechanisms are activated by the addition of Core-Shell particles. After blending 50vol% quartz with Bis-GMA/TEGDMA resin matrix, the experimental resins were made by the addition of Core-Shell particles with varied content level as 0, 2.5, 5, 7.5, 10, 12.5, 15, and 20wt%. Fracture toughness was determined on three-point bending specimen with single-edge notch according to ASTM-E 399. Also, flexural properties, that is, strength and modulus were measured by three-point bending testing. Fractogragh of fracture toughness specimen was observed using SEM (JEOL 6400 SEM, MA). The following results from this study were obtained ; 1. Fracture toughness of composite resin added 2.5wt% Core-Shell particles was significantly higher than control group ($p{\leq}0.05$). 2. Flexural properties were decreased with increasing Core-Shell particle content, which showed a correlation statistically ($p{\leq}0.05$). 3. A toughening mechanism such as lamination and microcrack was observed in specimen determined high fracture toughness. 4. The dispersion of Core-Shell itself and quartz filler particles was limited present high content of Core-Shell particles, which decreased a resulting mechanical properties of composites. These results suggest that adequate Core-Shell particles can be used to enhance mechanical properties included toughening for dental composites.