• Bulletin of the Korean Chemical Society
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• 제28권5호
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• pp.730-736
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• 2007

The interaction of a polyoxometal (POM), K6SiW11Co(H2O)O39.10H2O (K6) as a Keggin, with human serum albumin (HSA) was studied by different methods and techniques. Binding studies show two sets of binding sites for interaction of POM to HSA. Binding analysis and isothermal calorimetery revealed that, the first set of binding site has lower number of bound ligand per mole of protein (ν), lower Hill constant (n), higher binding constant (K), more negative entropy (ΔS) and more electrostatic interaction in comparison to the second set of binding site. In addition, differential scanning calorimetery (DSC) and spectrophotometery data showed that, there are two energetic domains. The first domain is less stable (lower Tm and Cp) which corresponds to the tail segment of HSA and another with more stability is related to the head segment of HSA. Polyoxometal also decreases the stability of protein as Tm, secondary and tertiary structure as well as quenching of the fluorescence decrease. On other hand, perturbations in tertiary structure are more than secondary structure.

• The Korean Journal of Physiology and Pharmacology
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• 제9권4호
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• pp.223-230
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• 2005

The purpose of the present study was to examine the effect of naltrexone, an opioid antagonist, on secretion of catecholamines (CA) evoked by cholinergic nicotinic stimulation and membrane-depolarization from the isolated perfused rat adrenal gland and to establish the mechanism of its action. Naltrexone $(3{\times}10^{-6}M)$ perfused into an adrenal vein for 60 min produced time-dependent inhibition in CA secretory responses evoked by ACh $(5.32{\times}10^{-3}M)$ , high $K^+$ $(5.6{\times}10^{-2}M)$ , DMPP ($10^{-4}$ M) and McN-A-343 $(10^{-4}M)$ . Naltrexone itself did also fail to affect basal CA output. In adrenal glands loaded with naltrexone $(3{\times}10^{-6}M)$ , the CA secretory responses evoked by Bay-K-8644, an activator of L-type $Ca^{2+}$ channels and cyclopiazonic acid, an inhibitor of cytoplasmic $Ca^{2+}-ATPase$, were also inhibited. However, in the presence of met-enkephalin $(5{\times}10^{-6}M)$ , a well-known opioid agonist, the CA secretory responses evoked by ACh, high $K^+$, DMPP, McN-A-343, Bay-K-8644 and cyclopiazonic acid were also significantly inhibited. Collectively, these experimental results demonstrate that naltrexone inhibits greatly CA secretion evoked by stimulation of cholinergic (both nicotinic and muscarinic) receptors as well as that by membrane depolarization. It seems that this inhibitory effect of naltrexone does not involve opioid receptors, but might be mediated by blocking both the calcium influx into the rat adrenal medullary chromaffin cells and the uptake of $Ca^{2+}$ into the cytoplasmic calcium store, which are at least partly relevant to the direct interaction with the nicotinic receptor itself.

• Archives of Pharmacal Research
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• 제28권6호
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• pp.699-708
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• 2005

The present study was designed to investigate the effect of naloxone, a well known opioid antagonist, on the secretion of catecholamines (CA) evoked by cholinergic stimulation and membrane-depolarization in the isolated perfused rat adrenal glands, and to establish its mechanism of action. Naloxone ($10^{-6}\~10^{-5}$ M), perfused into an adrenal vein for 60 min, produced dose- and time-dependent inhibition of CA secretory responses evoked by ACh ($5.32\times10^{-3}$ M), high K+ ($5.6\times10^{-2}$ M), DMPP ($10^{-4}$ M) and McN-A-343 ($10^{-4}$ M). Naloxone itself also failed to affect the basal CA output. In adrenal glands loaded with naloxone ($3\times10^{-6}$ M), the CA secretory responses evoked by Bay-K-8644, an activator of L-type $Ca^{2+}$ channels, and cyclopiazonic acid, an inhibitor of cytoplasmic $Ca^{2+}$-ATPase, were also inhibited. In the presence of met-enkephalin ($5\times10^{-6}$ M), a well known opioid agonist, the CA secretory responses evoked by ACh, high $K^+$, DMPP, McN-A-343, Bay-K-8644 and cyclopiazonic acid were also significantly inhibited. Taken together, these results suggest that naloxone greatly inhibits the CA secretion evoked by stimulation of cholinergic (both nicotinic and muscarinic) receptors as well as that by membrane depolarization. It seems that these inhibitory effects of naloxone does not involve opioid receptors, but might be mediated by blocking both the calcium influx into the rat adrenal medullary chromaffin cells and the uptake of $Ca^{2+}$ into the cytoplasmic calcium store, which are at least partly relevant to the direct interaction with the nicotinic receptor itself.

• Communications for Statistical Applications and Methods
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• 제19권1호
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• pp.193-211
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• 2012

This paper deals with robust nonparametric regression analysis when the regressors are functional random fields. More precisely, we consider $Z_i=(X_i,Y_i)$, $i{\in}\mathbb{N}^N$ be a $\mathcal{F}{\times}\mathbb{R}$-valued measurable strictly stationary spatial process, where $\mathcal{F}$ is a semi-metric space and we study the spatial interaction of $X_i$ and $Y_i$ via the robust estimation for the regression function. We propose a family of robust nonparametric estimators for regression function based on the kernel method. The main result of this work is the establishment of the asymptotic normality of these estimators, under some general mixing and small ball probability conditions.

• Coupled systems mechanics
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• 제1권1호
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• pp.79-98
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• 2012

In the present paper, the three-dimensional model of a typical rectangular concrete tank is excited using an artificial and a natural three components earthquake ground motion and the staggered displacement method is utilized for solving the coupled problem of the tank-contained liquid system in time domain. In the proposed method, surface sloshing of the liquid is taken into account in addition to the impulsive term and the appropriate damping values are applied on both of them. The resulted responses are compared with those obtained from the ABAQUS finite element software. It is found that the convective term affects responses extensively and must be considered in seismic design/safety assessment of storage tanks. In addition, the utilized method for solving the coupled problem is stable during the conducted general dynamic analyses and is able to capture the expected phenomena.

• Journal of Magnetics
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• 제14권1호
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• pp.23-26
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• 2009

Spin injection from a ferromagnet into an n-doped $In_{0.53}Ga_{0.47}As$ channel was electrically detected by a ferromagnetic detector. At T = 20 K, using non-local and local spin-valve measurements, a non-local signal of $2\;{\mu}V$ and a local spin valve signal of 0.041% were observed when the bias current was 1 mA. The band calculation and Shubnikov-de Haas oscillation measurement in a bulk channel showed that the gate controlled spin-orbit interaction was not large enough to control the spin precession but it could be a worthy candidate for a logic device using spin accumulation and diffusion.

• Macromolecular Research
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• 제15권3호
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• pp.225-233
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• 2007

To improve the poor mechanical and low-temperature properties of glycidyl azide polymer (GAP)-based propellants, the addition of binders was investigated using GAP and flexible polymer backbone-structural polycaprolactone (PCP) at various weight(wt) ratios, and varying the ratio of Desmodur N-100 pluriisocyanate (N-100) to isophorone diisocyanate (IPDI). Using Gee's theory, the solubility parameter of the PCP network was determined, in order to elucidate the physical and chemical interaction between GAP and PCP. The structure of the binder networks was characterized by measuring the cross-link densities and molecular weights between cross-links ($M_c$) obtained by a swelling experiment using Flory-Rhener theory. The thermal and mechanical properties of the segmented block copolyurethane (GAP-b-PCP) binders prepared by the incorporation of PCP into the binder recipes were investigated, along with the effect of the different curatives ratios.

• 한국멀티미디어학회논문지
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• 제20권8호
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• pp.1258-1270
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• 2017

'Semantic gap' has been a year-old problem in automatic video summarization, which refers to the gap between semantics implied in video summarization algorithms and what people actually infer from watching videos. Using the external EEG bio-feedback obtained from video watchers as a solution of this semantic gap problem has several another issues: First, how to define and measure noises against ERP waveforms as signals. Second, whether individual differences among subjects in terms of noise and SNR for conventional ERP studies using still images captured from videos are the same with those differently conceptualized and measured from videos. Third, whether individual differences of subjects by noise and SNR levels help to detect topic-irrelevant shots as signals which are not matched with subject's own semantic topical expectations (mis-match negativity at around 400m after stimulus on-sets). The result of repeated measures ANOVA test clearly shows a 2-way interaction effect between topic-relevance and noise level, implying that subjects of low noise level for video watching session are sensitive to topic-irrelevant visual shots, while showing another 3-way interaction among topic-relevance, noise and SNR levels, implying that subjects of high noise level are sensitive to topic-irrelevant visual shots only if they are of low SNR level.

• 대기
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• 제31권1호
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• pp.45-60
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• 2021

The synoptic structures and precipitation impact of extratropical cyclones (ETCs) influencing on the three adjacent megacities in East Asia, i.e., Beijing (Beijing ETCs), Seoul (Seoul ETCs) and Tokyo (Tokyo ETCs), are analyzed using ERA-interim reanalysis data from 1979 to 2018. Individual ETC tracks are identified with the automated tracking algorithm applied to 850-hPa relative vorticity field. Among four seasons, ETCs are the most frequent in spring. In this season, Beijing ETCs are mainly generated at the leeside of Altai-Sayan Mountains and primarily develop through interaction between the upper-level trough and lower-level cyclonic circulation. For Seoul ETCs, the leesides of Altai-Sayan Mountains (Seoul-N ETCs) and Tibetan Plateau (Seoul-S ETCs) are main genesis regions and the features of ETCs are different according to the genesis regions. While Seoul-N ETCs mainly develope by the same mechanism of Beijing ETCs, strong diabatic heating due to vapor transport is responsible for the genesis of Seoul-S ETCs. Tokyo ETCs are originated from the leesides of Tibetan Plateau and Kuroshio-Oyashio Extension regions, and strong diabatic heating as well as interaction between upper and lower levels determines the genesis of these ETCs. The precipitation impact resulting from ETCs become strong in the order of Beijing ETCs, Seoul-N ETCs, Seoul-S ETCs, and Tokyo ETCs and accounts for up to 40%, 27%, 52%, and 70% of regional precipitation, respectively.

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 1998년도 가을 학술발표논문집 Vol.25 No.2 (1)
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• pp.608-610
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• 1998

분산 M/VC 응용 프로그램의 조립식 작성기, DOC(Distributed Object Composer)는 M/VC(Model/View.Controller) 모델 기반 실시간 클라이언트/서버 응용의 신속한 개발을 지원한다. 본 논문에서는 DOC를 이용한 분산 M/VC 응용 프로그램의 조립식 작성 과정과 함께 그 구현을 소개한다. DOC는 개방화 및 표준화를 주도하는 CORBA 미들웨어를 기반으로 하여, 이질적인 통신 환경에서 클라이언트와 서버 객체간의 네트워크 투과성을 제공하고, 분산된 객체들 간의 상호작용(interaction) 패턴을 추상화하여 조립식 분산 응용 프로그램의 작성을 가능하게 한다. 분산 객체들 간의 상호작용을 처리하기 위해 자바의 관찰자/피관찰자(Observer/Observable) 패턴을 분산 관찰자/피관찰자 패턴으로 확장하여, serverDOC와 clientDOC로 구성된 DOC의 구현에 사용한다. 분산 피관찰자는 M/VC의 모델에 해당하고, 분산 관찰자는 뷰.콘트롤러에 해당한다. 개발자는 분산 환경을 고려하지 않고 필요한 관찰자/피관찰자 객체들을 생성하여 로컬상의 DOC에 조립식으로 연결하기만 하면 피관찰자의 상태 변화는 곧 관찰자에게 전달되고, 관찰자를 통한 사용자의 입력은 피관찰자에게 전달되어 반영된다. DOC를 이용한 응용 프로그램의 작성은 구성 객체들을 DOC에 plug-and-play 방식으로 조립하는 과정이다. 따라서 DOC는 분산 환경하의 컴포넌트 재사용성을 개선하고, 분산 응용의 생산성을 높인다.