• Archives of Pharmacal Research
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• v.21 no.4
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• pp.423-428
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• 1998

The ligand binding signals to a wide variety of seven transmembrane cell surface receptors are transduced into intracellular signals through heterotrimeric G-proteins. Recently, there have been reports which show diverse coupling patterns of ligand-activated receptors to the members of Gq family $\alpha$ subunits. In order to shed some light on these complex signal processing networks, interactions between G$\alpha$q family of G protein and neurokinin-2 receptor as well as muscarinic M$_{1}$ receptor, which are considered to be new thearpeutic targets in asthma, were studied. Using washed membranes from Cos-7 cells co-transfected with different G.alpha.q and receptor cDNAs, the receptors were stimulated with various concentrations of carbachol and neurokinin A and the agonist-dependent release of [$^3H$]inositol phosphates through phospholipase C beta-1 activation was measured. Differential coupling of Gaq family of G-protein to muscarinic M$_{1}$ receptor and neurokinin-2 receptor was observed. The neurokinin-2 receptor shows a ligand-mediated response in membranes co-transfected with G$\alpha$q, G$\alpha$11 and G$\alpha$14 but not G$\alpha$16 and the ability of the muscarinic $M_1$ receptor to activate phospholipase C through G$\alpha$/11 but not G$\alpha$14 and G$\alpha$16 was demonstrated. Clearly G$\alpha$/11 can couple $\M_1$ and neurokinin-2 receptor to activate phospholipase C. But, there are differences in the relative coupling of the G$\alpha$14 and G$\alpha$16 subunits to these receptors.

• Journal of Food Hygiene and Safety
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• v.29 no.1
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• pp.16-20
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• 2014

The prevalence of Bacillus cereus was determined in salad and Kimbab obtained from commercial retailers. Among the 100 salad samples analyzed, 54 samples were negative for B. cereus, whereas the bacterial count was < 10 colony forming units (CFU)/g in 8 samples, < 100 CFU/g in 25 samples, < 1,000 CFU/g in 11 samples, and > 1,000 CFU/g in 2 samples. The mean (standard deviation) was 1.18 log CFU/g (${\pm}0.71$ log CFU/g). In Kimbab, B. cereus was isolated from 20 samples; the mean bacterial count was 1.01 log CFU/g (${\pm}0.71$ log CFU/g). On the basis of the monitoring data, a statistical sampling plan was determined with the NEW sampleplan program (ICMSF), which was used as an analytical tool. To identify the most suitable sampling plan, the microbial limits (m, M) and the maximum allowable number of sample units yielding unsatisfactory test results (c) were varied, but the number of samples units, n = 5, was fixed. Sampling plans showing an acceptable probability (Pa) over 0.95 were considered suitable. Two plans (A and B) were finally suggested. Parameters for plan A are n = 5, c = 0, m = 1,000, and M = 10,000 and for plan B are n = 5, c = 2, m = 100, and M = 1,000. Interestingly, the latter plan was identical to the microbial sampling plan used in New Zealand. Thus, it was concluded that the suggested plan can be used as a sampling plan that is in line with international standards.

• Analytical Science and Technology
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• v.8 no.4
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• pp.435-442
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• 1995

A matrix modification was studied for the determination of trace bismuth in water samples by graphite furnace atomic absorption spectrophotometry. The type and quantity of modifiers as well as the use of auxiliary modifiers were investigated to realize the efficient modification. Palladium was chosen as a single modifier. By the addition of palladium($5{\mu}g/mL$) to 100 ng/mL bismuth solution, the temperatures could be raised from $500^{\circ}C$ to $1,300^{\circ}C$ for the charring and from $2,000^{\circ}C$ to $2,200^{\circ}C$ for the atomization as well as the sensitivity and reproducibility were improved. The absorbance of bismuth was maximum and not changed in the range of Pd $3-25{\mu}g/mL$. And several materials were examined as an auxiliary modifier. The mixed solution of $1{\mu}g/mL$ palladium and $200{\mu}g/mL$ nickel have raised the temperatures as with $5{\mu}g/mL$ palladium only. The maximum absorbance of bismuth was shown in the nickel concentration range of $100-300{\mu}g/mL$ in $1{\mu}g/mL$ palladium modified system. With such optimum conditions, the trace amount of bismuth in several water samples could be determined by a calibration curve method, and good recoveries were also obtained.

• Bulletin of the Korean Mathematical Society
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• v.47 no.6
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• pp.1275-1283
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• 2010

If the Wiener-Hopf $C^*$-algebra W(G,M) for a discrete group G with a semigroup M has the uniqueness property, then the structure of it is to some extent independent of the choice of isometries on a Hilbert space. In this paper we show that if the Wiener-Hopf $C^*$-algebra W(G,M) of a partially ordered group G with the positive cone M has the uniqueness property, then (G,M) is weakly unperforated. We also prove that the Wiener-Hopf $C^*$-algebra W($\mathbb{Z}$, M) of subsemigroup generating the integer group $\mathbb{Z}$ is isomorphic to the Toeplitz algebra, but W($\mathbb{Z}$, M) does not have the uniqueness property except the case M = $\mathbb{N}$.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.24 no.9 s.180
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• pp.2326-2333
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• 2000

Creep tests for type 316L(N) stainless steel were carried out using constant-load creep machines at 55$0^{\circ}C$, 575$^{\circ}C$ and $600^{\circ}C$. Material constants necessary to predict creep rupture time were obtained from the experimental creep data. And the applicability of Monkman-Grant(M-G) and modified M-G relationships was discussed. The log-log plot of M-G relationship between the rupture time($t_r$,) and the minimum creep rate ($ $\varepsilon$ _m$) was dependent on test temperatures. The slope of m was 1,05 at 55$0^{\circ}C$ and m was 1.30 at $600^{\circ}C$. On the other hand, the log-log plot of modified M-G relationship between $t_r/$\varepsilon$_r$, and $ $\varepsilon$ _m$ was independent on stresses and temperatures. That is, the slope of m＇ was approximately 1.35 in all the data. Thus, modified M-G relationship for creep life prediction could be utilized more reasonably than that of M-G relationship for type 316L(N) stainless steel. It was analyzed that the constant slopes regardless of temperatures or applied stresses in the modified relationship were due to an intergranular fracture grown by wedge-type cavities.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.11
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• pp.1749-1756
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• 2014

This study was performed to establish extraction conditions for useful components from sweet potato leaves. Extraction conditions were heating temperature ($40{\sim}60^{\circ}C$), ratio of solvent to sample (10~30 mL/g), and ethanol concentrations (50~90%). The maximum lutein content was $381.88{\mu}g/g$ under the following conditions: 81.23% ethanol concentration, $46.75^{\circ}C$ temperature, and 25.78 mL/g ratio of solvent. The maximum total polyphenol content was 58.44 mg GAE/g under 67.0%, $53.90^{\circ}C$, and 29.10 mL/g, whereas maximum total chlorophyll content was 769.14 mg/100 g under 93.10%, $69.90^{\circ}C$, and 25.98 mL/g, respectively. The highest antioxidant activity of electron-donating ability ($IC_{50}$) was 0.582 mg/mL under 64.56%, $54.63^{\circ}C$, and 19.57 mL/g, whereas total antioxidant activity was 32.54 mg AA eq/g under 64.42%, $44.51^{\circ}C$, and 26.01 mL/g, respectively. Under these conditions, experimental data matched well with the predicted data. Consequently, the present study was carried out to simulate and optimize sweet potato leaves for useful component extraction.

• Journal of Mechanical Science and Technology
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• v.16 no.11
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• pp.1420-1427
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• 2002

The Monkman-Grant (M-G) and its modified parameters were evaluated for type 316LN and modified 9Cr-Mo stainless steels prepared with minor element variations. Several sets of creep data for the two alloy systems were obtained by constant-load creep tests in 550～650$\^{C}$ temperature range. The M-G parameters, m, m', C, and C' were proposed and discussed for the two alloy systems. The m value of the M-C relation was 0.90 in type 316LN steel and 0.84 in modified 9Cr-Mo steel. The m' value of the modified relation was 0.94 in type 316LN steel and 0.89 in 9Cr-Mo steel. Although creep fracture modes and creep properties between type 316LN and modified 9Cr-Mo steels showed a basic difference, the M-G and its modified relations demonstrated linearity quite well. The m' of modified relation almost overlapped regardless of the creep testing conditions and chemical variations in the two alloy systems, and the parameter m' was closer to unity than that of the M-G relation.

• Kyungpook Mathematical Journal
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• v.45 no.1
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• pp.45-53
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• 2005

A basis of the cycle space C (G) is d-fold if each edge occurs in at most d cycles of C(G). The basis number, b(G), of a graph G is defined to be the least integer d such that G has a d-fold basis for its cycle space. MacLane proved that a graph G is planar if and only if $b(G)\;{\leq}\;2$. Schmeichel showed that for $n\;{\geq}\;5,\;b(K_{n}\;{\bullet}\;P_{2})\;{\leq}\;1\;+\;b(K_n)$. Ali proved that for n, $m\;{\geq}\;5,\;b(K_n\;{\bullet}\;K_m)\;{\leq}\;3\;+\;b(K_n)\;+\;b(K_m)$. In this paper, we give an upper bound for the basis number of the semi-strong product of a bipartite graph with a cycle.

• Journal of the Chungcheong Mathematical Society
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• v.30 no.1
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• pp.77-102
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• 2017

As an analogy of $Poincar{\acute{e}}$ series in the space of modular forms, T. Ono associated a module $M_c/P_c$ for ${\gamma}=[c]{\in}H^1(G,R^{\times})$ where finite group G is acting on a ring R. $M_c/P_c$ is regarded as the 0-dimensional twisted Tate cohomology ${\hat{H}}^0(G,R^+)_{\gamma}$. In the case that G is the Galois group of a Galois extension K of a number field k and R is the ring of integers of K, the vanishing properties of $M_c/P_c$ are related to the ramification of K/k. We generalize this to arbitrary n-dimensional twisted cohomology of the ring of integers and obtain the extended version of theorems. Moreover, some explicit examples on quadratic and biquadratic number fields are given.

• The korean journal of orthodontics
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• v.32 no.3 s.92
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• pp.227-234
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• 2002

In order to investigate the action mechanism of protein kinase C on $K^+$ channel in osteoblastic cell, effects of phorbol 12, 13-dibutyrate on human osteoblast-like cells (G292) were studied by patch clamp technique with cell-attacked configuration. 111 this experiment, 45pS ion channel was dominant in G292 cell line according to their approximate conductances in symmetrical 140mM KCl saline at holding potential of 60mV. In torrent-voltage relationship, reversal potential was 5.5mV at the condition of potassium enriched saline in the pipette and -27 mV at the condition of standard extracellular saline In the pipette. Phorbol 12, 13-dibutyrate 10nM increased the open probability of 45pS channel and staurosporine, an inhibitor of protein kinase C, suppressed this effect. Phorbol 12,13-dibutyrate moved the reversal potential of 45pS channel to more negative potential and increased the single channel current at the same membrame potential. In order to check the activation of protein kinase C in G292 cell by phorbol 12,13-dibutyrate, western blot of protein kinase C was performed. Phorbol 12,13-dibutyrate $0.1{\mu}M$ translocated protein kinase C from cellular compartment to membrane compartment of the cell. These findings suggest that phorbol 12,13-dibutyrate, one of phorbol esters, activate 45pS channel In G292 cell and affect cell membrane potential, that regulate cellular function.