• Journal of Microbiology and Biotechnology
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• v.7 no.4
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• pp.250-257
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• 1997

A new sensing system based on the immobilization of luminescent bacteria, photobacterium phosphoreum, was proposed for continuous real-time monitoring of pollutants. The response curves demonstrate that Photobacterium phosphoreum immobilized on the strontium alginate were very sensitive to seven reference chemicals used. The significant inhibitory concentrations for bioluminescence emission were 5 ppm for Pb$(NO_3)_2$), $NiCl_2$, $CdCl_2$, 50 ppm for $NaASO_2$, 0.1 ppm for $HgCl_2$, 0.5 ppm for pentachlorophenol and less than 5 ppm for SDS, respectively. The alginate mixed-cells (AMC) retained their luminescence during experimental period (29 days) under storage condition of $-80^{\circ}C$. The variables affecting performance of continuous flow through monitoring (CFTM) was optimized in order to ensure stability and efficiency. The flow through cell with strontium-alginate immobilized luminescent bacteria was tested with salicylate and 4-nitrophenol. A rapid response of luminescence was recorded by time drive mode in bioluminescence spectrometer after exposure to both toxicants.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.13 no.2
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• pp.156-163
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• 2008

Luminescent bacterial toxicity test was first introduced in the early 1980s, registered as international standard method in 1998 and now widely used as a common toxicity test method. This toxicity test uses luminescent bacterium, Vibrio fischeri, originated from marine environment as a test organism. The degree of toxicity can be evaluated from the comparison of luminescent emission intensity between control and treatment groups to toxicants and materials from various environmental matrix for 30 min. This test can be carried out by using commercial products and its results are sensitive and precise. This research is on the feasibility of adopting luminescent bacterial test as a domestic standard test protocol. Using commercial products, a series of experiments were conducted to identify the precision and accuracy of injection volume and light emission, and to evaluate concentration-response relationship between chemical concentrations and light emissions. Also, the feasibility of the application to environmental media and quality assurance/quality control were checked. The results of serial toxicity tests revealed that the preliminary luminescent bacterial toxicity test was robust and suitable as a standard method.

• Journal of Wetlands Research
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• v.9 no.1
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• pp.21-29
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• 2007

In Korea, we assign the chemical substances of 535 types as toxic substance. Only 10% of the 535 toxic substances are being managed by the Ministry of Environment related with water quality standard. Tar color is also one of chemical substances, but we have the lacks for the information of tar colors about the environmental effects of aquatic ecosystem. This study performed the test of bioassay using Water Fleas and Luminescent Bacteria. The tar has 7 types of colors allowed as the edible color and we evaluate the toxicities of 5 tar colors out of 7 colors and we would like to provide the informations for further study as we perform the toxicity test for the samples of 5 tar colors. We did the toxicity test of using Water Fleas From the results, we obtained the magnitudes of toxicity in order of Red No.2, Yellow No.5, Red No.3, Yellow No.4, Blue No.1. As the result based on Microtox Acute Toxicity Test using Luminescent Bacteria with the standard of 15min-EC50, we obtained in order of Yellow No.5, Food Red No.3, Red No.2, Yellow No.4, Blue No.1. We could expect the tar colors may have different effects on the aquatic ecosystem, respectively and it may influence to the aquatic ecosystem and the human, because of bioconcentration by food chain when toxicity of the tar colors overflow in the aquatic ecosystem.

• Food Science of Animal Resources
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• v.37 no.1
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• pp.147-152
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• 2017

A bioluminescent Lactobacillus plantarum (pLuc2) strain was constructed. The luminescent signal started to increase during the early exponential phase and reached its maximum in the mid-exponential phase in a batch culture of the strain. The signal detection sensitivity of the strain was the highest in PBS (phosphate buffered saline), followed by milk and MRS broth, indicating that the sensitivity was influenced by the matrix effect. The strain was used in millet seed fermentation which has a complex matrix and native lactic acid bacteria (LAB). The luminescent signal was gradually increased until 9 h during fermentation and abolished at 24 h, indicating that the strain could be specifically tracked in the complex matrix and microflora. Therefore, the bioluminescent labeling system can be used for monitoring LAB in food and dairy sciences and industries.

• Biotechnology and Bioprocess Engineering:BBE
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• v.2 no.2
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• pp.141-146
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• 1997

A new sensing system based on the immobilization of luminescent batcteria, Photobacterium phosphoreum, was proposed for continuous real-time monitoring of polluants. The response curves demonstrate that Photobacterium phosphoreum immobilized on the strontium alginate was very sensitive to seven reference chemicals used. The significant inhibitory concentrations for bioluminescence emission were 5 ppm for Pb(NO3)2, NiCl2, CdCl2, 50 ppm for NaAsO2, 0.1ppm for HgCl2, 0.5ppm for pentachlorophenol and less than 5ppm for SDS, respectively. The alginate mixed-cells (AMC) retained their luminescence during experimental period (29 days) under storage condition of -8$0^{\circ}C$. The variables affecting performance of continuous flow through monitoring (CFTM) were optimized in order to ensure stability and efficiency. The flow through cell with strontium-alginate immobilized luminescent bacteria was tested with salicylate and 4-nitrophenol and a rapid response of luminescence was recorded by time drive mode in bioluminescence spectrometer after exposure to both toxicants.

• Journal of Microbiology and Biotechnology
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• v.7 no.4
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• pp.242-249
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• 1997

Stability of bioluminescence was investigated with Photobacterium phosphoreum immobilized on the strontium alginate in order to develope continuous real time monitoring of pollutants. The stability of bioluminescence emission was improved by prolonged aging time. The aging time of ${\geq}40$ min and the cell concentration of ${\leq}0.6\;of\;OD_660$ were selected for the immobilization of P. phosphoreum to give linearity between cell concentrations and bioluminescence intensity. In sensitivity tests using phenol, it was found that this compound quenched bioluminescence proportional to the concentration without lowering of cell growth. The lower value for maximum quenching ($q_s$) and higher dissociation constant ($K_s$) were observed with strontium-alginate immobilized cells compared to free cells. The response of bioluminescence to toxicants was evaluated with the immobilized luminescent bacteria. The sensitivity of the immobilized cells was found to be good in response to toxicants, 4-nitrophenol, salicylate and cadmium, when evaluated with a specific rate of bioluminescence quenching.

• Microbiology and Biotechnology Letters
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• v.12 no.4
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• pp.265-270
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• 1984

Luminous marine bacteria which have ability toproduce pyruvic acid were isolated from fresh fishes. Among them, newly isolated bacterium BL-1980 having the highest ability to produce pyruvic acid was selected and identified as Beneckea sp. Optimal conditions for the production of pyruvic acid from glucose by the bacterium BL-1980 were investigated. At the optimal conditions, 10.6g/L of pyruvic acid was produced at the conversion ratio of 35.33%.

• Journal of Marine Life Science
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• v.7 no.2
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• pp.74-85
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• 2022

Organisms constituting a large proportion of marine ecosystems, ranging from bacteria to fish, exhibit fluorescence and bioluminescence. A variety of marine organisms utilize these biochemically generated light sources for feeding, reproduction, communication, and defense. Since the discovery of green fluorescent protein and the luciferin-luciferase system more than a century ago, numerous studies have been conducted to characterize their function and regulatory mechanism. The unique properties of fluorescent and bioluminescent proteins offer great potential for their use in a broad range of applications. This short review briefly describes the functions and characteristics of fluorescent and bioluminescent proteins, in addition to summarizing the recent status of their applications.

• Journal of the Korean Society of Marine Environment & Safety
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• v.25 no.1
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• pp.81-88
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• 2019

In order to understand the effects of the main components of antifoaming agents on the marine benthic ecosystem when silicone-based antifoaming agents are discharged into marine environments, eco-toxicity testing was performed on silicone and alcohol-based antifoaming agent by using benthic amphipod (Monocorophium acherusicum) and luminescent bacteria (Vibrio fischeri). The toxic effects of Polydimethylsiloxane (PDMS) as a main component of silicone-based antifoaming agents on aquatic organisms were also researched. In the results of the eco-toxicity test, luminescent bacteria showed a maximum of 9 times more toxic effects than benthic amphipod for alcohol-based antifoaming agents, and silicone-based antifoaming agents showed a maximum of 400 times more toxic effects than alcohol-based. The $LC_{50}$ and $EC_{50}$ values of PDMS ranged from 10 to $44,500{\mu}g/L$ in phytoplankton, invertebrate, and fish. In the results of applying PBT (P: persistency, B: bioaccumulation, T: toxicity) characteristics as an index showing the qualitative characteristics of PDMS, persistency (P) and bioaccumulation (B) were confirmed. Thus, when PDMS is discharged to marine environments, it could accumulate in the upper trophic level through bioaccumulation and the food chain, which could have negative effects on benthic organisms. The results of this study may be used for objective and scientific risk assessment, considering the major components of antifoaming agents when investigating the effects of various discharged antifoaming agents in marine ecosystem.

• Journal of Environmental Health Sciences
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• v.49 no.2
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• pp.89-98
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• 2023

Background: Organophosphorus flame retardants (OPFRs) are a group of chemical substances used in building materials and plastic products to suppress or mitigate the combustion of materials. Although OPFRs are generally used in mixed form, information on their mixture toxicity is quite scarce. Objectives: This study aims to elucidate the toxicity and determine the types of interaction (e.g., synergistic, additive, and antagonistic effect) of OPFRs mixtures. Methods: Nine organophosphorus flame retardants, including TEHP (tris(2-ethylhexyl) phosphate) and TDCPP (tris(1,3-dichloro-2-propyl) phosphate), were selected based on indoor dust measurement data in South Korea. Nine OPFRs were exposed to the luminescent bacteria Aliivibrio fischeri for 30 minutes and the human hepatocyte cell line HepG2 for 48 hours. Chemicals with significant toxicity were only used for mixture toxicity tests in HepG2. In addition, the observed EC_x values were compared with the predicted toxicity values in the CA (concentration addition) prediction model, and the MDR (model deviation ratio) was calculated to determine the type of interaction. Results: Only four chemicals showed significant toxicity in the luminescent bacteria assays. However, EC₅₀ values were derived for seven out of nine OPFRs in the HepG2 assays. In the HepG2 assays, the highest to lowest EC₅₀ were in the order of the molecular weight of the target chemicals. In the further mixture tests, most binary mixtures show additive interactions except for the two combinations that have TPhP (triphenyl phosphate), i.e., TPhP and TDCPP, and TPhP and TBOEP (tris(2-butoxyethyl) phosphate). Conclusions: Our data shows OPFR mixtures usually have additivity; however, more research is needed to find out the reason for the synergistic effect of TPhP. Also, the mixture experimental dataset can be used as a training and validation set for developing the mixture toxicity prediction model as a further step.